FastQCFastQC Report
Thu 26 May 2016
SRR936723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084419
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.43912915579679074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.3415653912371509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.20647000836392573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.14219595931093057No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10990.10134459097452184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17050.052.3831252
GTACCTG17500.051.848141
ACCTGGG23000.035.9401053
GTACACG2350.034.3652081
GGTATCA38900.032.606151
GTATCAA58000.030.3048151
CGTATCC700.00838341527.148138
GTACATG43700.026.8505651
TCACGCA1606.2322215E-826.7227064
TATCACG1606.2322215E-826.7227062
CCTGGGG15700.026.0229634
ATCACGC1658.397183E-825.9129283
TACATGG44300.025.6302052
ACATGGG44700.024.6568973
ATCAACG70800.024.5587223
CAACGCA71700.024.2504545
TATCAAC71650.024.201072
TCAACGC71950.024.1001644
TTCAACG2202.1645974E-923.753522
AACGCAG73700.023.6568286
Produced by FastQC (version 0.11.5)