##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936723_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1084419 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.125610119335793 31.0 31.0 34.0 28.0 34.0 2 31.165319862525465 31.0 31.0 34.0 27.0 34.0 3 31.158485788242366 31.0 31.0 34.0 27.0 34.0 4 34.81265544037867 37.0 35.0 37.0 32.0 37.0 5 34.824285631291964 37.0 35.0 37.0 32.0 37.0 6 34.81820218937514 37.0 35.0 37.0 32.0 37.0 7 34.723746079697975 37.0 35.0 37.0 31.0 37.0 8 34.678427803275305 37.0 35.0 37.0 31.0 37.0 9 36.12872238498219 39.0 35.0 39.0 31.0 39.0 10-11 35.96914246246146 39.0 35.0 39.0 30.0 39.0 12-13 35.84743351047888 38.0 35.0 39.0 30.0 39.0 14-15 36.86241941537358 39.0 36.0 41.0 30.5 41.0 16-17 36.748673252681854 39.0 36.0 41.0 30.0 41.0 18-19 36.72007867807554 39.0 36.0 41.0 30.0 41.0 20-21 36.573514019949855 39.0 36.0 41.0 30.0 41.0 22-23 36.657076277711845 39.0 36.0 41.0 30.0 41.0 24-25 36.679829014430766 39.0 36.0 41.0 30.0 41.0 26-27 36.56721433320516 39.0 36.0 41.0 30.0 41.0 28-29 36.39676407366525 39.0 35.5 41.0 30.0 41.0 30-31 36.14401767213595 39.0 35.0 40.5 29.0 41.0 32-33 35.885696395950276 38.5 35.0 40.0 28.0 41.0 34-35 35.64521831506087 38.0 35.0 40.0 27.0 41.0 36-37 35.39082587081192 38.0 34.5 40.0 26.0 41.0 38-39 35.16008387901724 38.0 34.0 40.0 25.5 41.0 40-41 34.91480645396291 38.0 34.0 40.0 25.0 41.0 42-43 34.63440976227824 38.0 33.5 40.0 24.0 41.0 44-45 34.329462597022 38.0 33.0 40.0 23.5 41.0 46-47 34.06197189462745 38.0 33.0 40.0 22.5 41.0 48-49 33.84701761957325 38.0 33.0 40.0 20.5 41.0 50-51 32.87085619119547 37.0 31.5 39.5 17.5 40.5 52-53 32.96015654465663 37.5 32.5 39.5 18.0 40.0 54-55 33.369240579517694 38.0 33.0 40.0 18.0 41.0 56-57 33.26380624094561 38.0 33.0 40.0 16.5 41.0 58-59 33.01625386497285 37.0 32.0 40.0 14.0 41.0 60-61 32.72301112392904 37.0 32.0 40.0 12.5 41.0 62-63 32.32774831499633 36.0 31.0 39.5 8.5 41.0 64-65 31.966062933238906 36.0 31.0 39.0 8.0 41.0 66-67 31.51377373505997 35.0 31.0 39.0 7.0 40.5 68-69 31.11141726583544 35.0 30.0 38.5 4.0 40.0 70-71 30.70998018293667 35.0 30.0 38.0 2.0 40.0 72-73 30.307762497706143 35.0 29.0 37.0 2.0 39.5 74-75 29.872017181550675 34.5 29.0 36.5 2.0 39.0 76-77 29.400140075007908 34.0 29.0 36.0 2.0 39.0 78-79 28.898809408540426 34.0 28.0 35.5 2.0 37.5 80-81 28.450982046607443 34.0 27.0 35.0 2.0 37.0 82-83 28.02936272787548 34.0 26.5 35.0 2.0 37.0 84-85 27.643137016227122 33.5 26.0 35.0 2.0 36.0 86-87 27.251850069023135 33.0 26.0 35.0 2.0 36.0 88-89 26.83038290550055 33.0 25.0 35.0 2.0 35.5 90-91 26.405955631540944 33.0 24.5 35.0 2.0 35.0 92-93 25.93647704438967 32.5 23.5 35.0 2.0 35.0 94-95 25.40362765683744 32.0 20.0 35.0 2.0 35.0 96-97 24.773125978058296 32.0 14.5 35.0 2.0 35.0 98-99 23.965391144935676 32.0 2.0 34.0 2.0 35.0 100-101 21.077294846364737 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7176431795740363 1101 2 0.7366723927360539 1101 3 0.6682792328005824 1101 4 0.5871886764862566 1101 5 0.6619542446371938 1101 6 0.49611747632956593 1101 7 0.7381298230476361 1101 8 0.679420659024423 1101 9 0.7662002675402135 1101 10-11 0.8920343895847296 1101 12-13 0.8936761949800385 1101 14-15 0.8734160883327746 1101 16-17 1.0434231928270066 1101 18-19 0.823694851477029 1101 20-21 0.9657713161068884 1101 22-23 0.9539929209447067 1101 24-25 0.966189352169998 1101 26-27 0.8817764873755038 1101 28-29 0.9102189344589462 1101 30-31 0.8744894739326199 1101 32-33 0.9339120976335664 1101 34-35 0.9223762477000932 1101 36-37 0.8248074232293234 1101 38-39 0.6797452717954542 1101 40-41 0.8311708454470121 1101 42-43 1.026633080980318 1101 44-45 0.6838289425470592 1101 46-47 0.9355253790883182 1101 48-49 1.202736608893126 1101 50-51 1.2458823166823976 1101 52-53 1.4346240657410334 1101 54-55 1.3739920838372583 1101 56-57 1.3839971158535462 1101 58-59 1.3380854519410548 1101 60-61 1.3947586442472826 1101 62-63 1.4439821908837658 1101 64-65 1.348262717672437 1101 66-67 1.3533492773772302 1101 68-69 1.1053589964771717 1101 70-71 1.1300736803198816 1101 72-73 1.069305839553568 1101 74-75 1.0952396306739303 1101 76-77 1.2147385126930104 1101 78-79 1.13778174619544 1101 80-81 1.052113992953057 1101 82-83 0.9189171913187728 1101 84-85 0.8289038391461325 1101 86-87 1.0222177701597417 1101 88-89 1.135164083504236 1101 90-91 0.8707341642896935 1101 92-93 0.9136576495713378 1101 94-95 0.6995064579585417 1101 96-97 0.7081515487159358 1101 98-99 0.8143461642511411 1101 100-101 1.170312553623777 1102 1 0.5539596274007437 1102 2 0.6875423845716888 1102 3 0.6240426010134819 1102 4 0.6432995920187778 1102 5 0.6419725208324536 1102 6 0.6555682916426235 1102 7 0.5774408704084522 1102 8 0.7221157986051239 1102 9 0.8954024816563262 1102 10-11 0.6306158871841134 1102 12-13 0.6906860895923117 1102 14-15 0.90286023732871 1102 16-17 0.650219840193806 1102 18-19 0.6105060299171114 1102 20-21 0.680718472435764 1102 22-23 0.5094248458625046 1102 24-25 0.5522721043852385 1102 26-27 0.4854036380118032 1102 28-29 0.21173009117731567 1102 30-31 0.6244183166150279 1102 32-33 0.5027058687466948 1102 34-35 0.6109966945680512 1102 36-37 0.5254830432342672 1102 38-39 0.3774948279984969 1102 40-41 0.48059334763790673 1102 42-43 0.2809811675974956 1102 44-45 0.5601642657885364 1102 46-47 0.597578305152112 1102 48-49 0.6456725598170507 1102 50-51 0.7051728407747717 1102 52-53 0.6368157111229209 1102 54-55 0.5288041910676 1102 56-57 0.6934617881179221 1102 58-59 0.6682490388690923 1102 60-61 0.5206423057781464 1102 62-63 0.675297028308016 1102 64-65 0.6157127390196315 1102 66-67 0.7615222570711744 1102 68-69 0.5889553622108039 1102 70-71 0.5561427253886784 1102 72-73 0.5033928162663948 1102 74-75 0.4703079791500393 1102 76-77 0.6008549710060578 1102 78-79 0.5632148695522723 1102 80-81 0.7147058420695878 1102 82-83 0.7148350083620727 1102 84-85 0.40998086132474754 1102 86-87 0.3927170698219342 1102 88-89 0.6522178709460889 1102 90-91 0.4144869263171529 1102 92-93 0.5138063309138907 1102 94-95 0.2502251468662422 1102 96-97 0.00755120566276446 1102 98-99 0.10189850233079767 1102 100-101 0.4657719677368952 1103 1 0.353215760609487 1103 2 0.2649070167546377 1103 3 0.44568200328792784 1103 4 0.4916221263423708 1103 5 0.3930725282901264 1103 6 0.591791986878043 1103 7 0.5530052776912342 1103 8 0.5299833560986968 1103 9 0.17188345214878353 1103 10-11 0.5363208643796966 1103 12-13 0.19681747047638254 1103 14-15 0.5955892940494678 1103 16-17 0.7258896785669222 1103 18-19 0.810960363339646 1103 20-21 0.7148516051429468 1103 22-23 0.5977935739190201 1103 24-25 0.463771039107165 1103 26-27 0.43642753328846595 1103 28-29 0.32219621065515724 1103 30-31 0.3874670591189684 1103 32-33 0.3638223415868538 1103 34-35 0.6941084910056148 1103 36-37 0.7671066820253074 1103 38-39 0.7164897816365965 1103 40-41 0.7457507119483822 1103 42-43 0.5520037760591876 1103 44-45 0.8087871544249339 1103 46-47 0.7865668247153579 1103 48-49 0.8378185285035755 1103 50-51 0.7962179246297723 1103 52-53 1.0981710489922705 1103 54-55 1.1767893751479406 1103 56-57 1.0784680540294644 1103 58-59 0.9461795040673167 1103 60-61 0.8712068958471448 1103 62-63 0.9649689489817277 1103 64-65 0.8549086112681543 1103 66-67 0.8683304587618714 1103 68-69 0.8594065809561897 1103 70-71 0.6885238156426645 1103 72-73 0.641082383852698 1103 74-75 0.7220707694328325 1103 76-77 0.8806513179138271 1103 78-79 0.6352867440176198 1103 80-81 0.6846312945385868 1103 82-83 0.6426059377495648 1103 84-85 0.9505700032385036 1103 86-87 0.6448403145258865 1103 88-89 0.49735188248940787 1103 90-91 0.5843070208103143 1103 92-93 0.3818569817059405 1103 94-95 0.38863745115703807 1103 96-97 0.5933177891251447 1103 98-99 0.5113180610409991 1103 100-101 0.7041354315503447 1104 1 0.27016327915368166 1104 2 0.330670901267478 1104 3 0.298470712137064 1104 4 0.38876696189101523 1104 5 0.33470748821034135 1104 6 0.5303218223651172 1104 7 0.36196660741170206 1104 8 0.5209888529005013 1104 9 0.5245363525465052 1104 10-11 0.6835727169880528 1104 12-13 0.5987590605289697 1104 14-15 0.3253366323848965 1104 16-17 0.3439166388985413 1104 18-19 0.3696955267190276 1104 20-21 0.37545324744738906 1104 22-23 0.34049921145459194 1104 24-25 0.3517102345851342 1104 26-27 0.08912512372059211 1104 28-29 0.11006051229913538 1104 30-31 0.4122102724990313 1104 32-33 0.3287145893507173 1104 34-35 0.3948772030562111 1104 36-37 0.0717471300555772 1104 38-39 0.29866874313290737 1104 40-41 0.34305547552941107 1104 42-43 0.36460839383625654 1104 44-45 0.2532679351750531 1104 46-47 0.024695536360333392 1104 48-49 0.3411767998901496 1104 50-51 0.38247041535677795 1104 52-53 0.3581250841692025 1104 54-55 0.19635520820422414 1104 56-57 0.26819608221388336 1104 58-59 0.5904649379409221 1104 60-61 0.45944979523807206 1104 62-63 0.618328560345013 1104 64-65 0.5221145280258987 1104 66-67 0.30893653385416897 1104 68-69 0.22236136724438893 1104 70-71 0.2433151015929731 1104 72-73 0.13667458475359595 1104 74-75 -0.02113572967155619 1104 76-77 -0.041612286652526365 1104 78-79 0.2177170416880152 1104 80-81 0.15334501104731757 1104 82-83 -0.08756476079841136 1104 84-85 0.05031605954215124 1104 86-87 0.018296172433306168 1104 88-89 0.2656738912086709 1104 90-91 0.2867637413730151 1104 92-93 -0.25922952936408095 1104 94-95 -0.12446426176779823 1104 96-97 -0.003233328089500276 1104 98-99 0.004525103379190654 1104 100-101 0.0748997470286703 1105 1 0.3888385535844243 1105 2 0.20009462318613203 1105 3 0.14808626543732828 1105 4 0.16747086766043395 1105 5 0.18092174582599796 1105 6 0.40299075891640257 1105 7 0.2717136219201777 1105 8 0.3791945140520667 1105 9 0.46535267763579213 1105 10-11 0.46721506961682024 1105 12-13 0.12793915475672435 1105 14-15 0.31428807140275694 1105 16-17 0.20438759405102047 1105 18-19 0.35292599487036824 1105 20-21 0.26448668198621306 1105 22-23 0.05933473380702736 1105 24-25 0.030299092375528858 1105 26-27 0.002668580287334521 1105 28-29 -0.14341299735635715 1105 30-31 0.07609608331890882 1105 32-33 -0.17829210800952922 1105 34-35 -0.14626074787501153 1105 36-37 0.19330248833221475 1105 38-39 -0.09112885293638584 1105 40-41 -0.2376592626887657 1105 42-43 0.1769142194732396 1105 44-45 0.1441189017828819 1105 46-47 0.5237498688101496 1105 48-49 0.5002417543264883 1105 50-51 0.567118890459021 1105 52-53 0.7854064983059033 1105 54-55 0.7280922773128182 1105 56-57 0.41352555095211585 1105 58-59 0.4228458943575788 1105 60-61 0.4813184456172195 1105 62-63 0.29292569819989467 1105 64-65 0.22607255987914598 1105 66-67 0.526227489416268 1105 68-69 0.47452160669108423 1105 70-71 0.25641249122566023 1105 72-73 0.33712573668119816 1105 74-75 0.24833396906376137 1105 76-77 0.1208574947139418 1105 78-79 0.2783901959589521 1105 80-81 0.3048061345060198 1105 82-83 0.02533092409019133 1105 84-85 0.014791376489093011 1105 86-87 0.10425975399201093 1105 88-89 0.09220851754286485 1105 90-91 0.1522095152136913 1105 92-93 -0.07403759557634615 1105 94-95 0.16926474056352703 1105 96-97 0.10865030183314417 1105 98-99 0.6628435525126619 1105 100-101 0.6886923682792805 1106 1 0.27204923602864284 1106 2 0.19272742027836642 1106 3 0.24068768672072238 1106 4 0.23057495385018711 1106 5 0.3784732103020616 1106 6 0.16514379136795299 1106 7 0.375007266892716 1106 8 0.5875991560418612 1106 9 0.5347706360078348 1106 10-11 0.41664908262351474 1106 12-13 0.48978067024903993 1106 14-15 0.3049527495766071 1106 16-17 0.3768913835967638 1106 18-19 0.17793251451895742 1106 20-21 0.06992406891105674 1106 22-23 0.4759372226828731 1106 24-25 0.3546668902012229 1106 26-27 0.25944188395627066 1106 28-29 0.3665447471712113 1106 30-31 0.5693013870530379 1106 32-33 0.0909642853208723 1106 34-35 0.2723444955523675 1106 36-37 0.1354996914029769 1106 38-39 0.12027992088070505 1106 40-41 0.21223621919475733 1106 42-43 0.022023668073963165 1106 44-45 -0.16949147586776547 1106 46-47 -0.11097153845599905 1106 48-49 -0.04288266501730931 1106 50-51 -0.30274484888543185 1106 52-53 -0.37032142045927685 1106 54-55 -0.18279431339994545 1106 56-57 -0.10345507754200156 1106 58-59 0.13133508804004634 1106 60-61 0.12671272136575595 1106 62-63 0.21031563838212008 1106 64-65 0.09201432311811075 1106 66-67 0.08978896970248584 1106 68-69 0.07592760965752632 1106 70-71 0.0012191836449382265 1106 72-73 -0.18777524615582664 1106 74-75 0.35055187201879434 1106 76-77 0.1643078964872835 1106 78-79 0.24701948059051304 1106 80-81 0.44637113540246887 1106 82-83 0.30376677889761794 1106 84-85 0.39275883529306554 1106 86-87 0.39615460835936034 1106 88-89 0.262377457936978 1106 90-91 0.34796502024197196 1106 92-93 0.3071020797451567 1106 94-95 0.2691610374031299 1106 96-97 0.44009599316038006 1106 98-99 0.5847941906232634 1106 100-101 0.6454895094133697 1107 1 0.3264052060589293 1107 2 0.13584873384444407 1107 3 0.1730685252349886 1107 4 0.2289290109201687 1107 5 0.27200501326715454 1107 6 0.3485050220390846 1107 7 0.3471936211091986 1107 8 0.4044890014109086 1107 9 0.2627636218200422 1107 10-11 0.5192435350386333 1107 12-13 0.33398400198824163 1107 14-15 0.07069481876200712 1107 16-17 0.22829023582058028 1107 18-19 0.22426999259961633 1107 20-21 0.3682263166394222 1107 22-23 0.3364883943710595 1107 24-25 0.06447280443683923 1107 26-27 0.07155530656108056 1107 28-29 0.1825677824015699 1107 30-31 0.027913693764340053 1107 32-33 -0.19569879454522976 1107 34-35 0.06969225579467064 1107 36-37 0.09536339298116303 1107 38-39 -0.03649713953696221 1107 40-41 -0.07882666937529592 1107 42-43 0.04953044314171251 1107 44-45 0.12388290640547694 1107 46-47 0.12321664901411111 1107 48-49 0.09814435554701362 1107 50-51 0.14959055682157185 1107 52-53 0.12867029004606678 1107 54-55 0.15923900399204882 1107 56-57 0.04435498144093941 1107 58-59 0.16334728316199687 1107 60-61 0.16757435057028403 1107 62-63 0.07863621864674286 1107 64-65 0.22760734081299105 1107 66-67 0.228456672555982 1107 68-69 0.3056004137385102 1107 70-71 0.11980279595164944 1107 72-73 -0.09798567178949469 1107 74-75 -0.05770175463512217 1107 76-77 -0.13545497614144253 1107 78-79 -0.2803298737853872 1107 80-81 -0.20164217561299225 1107 82-83 -0.43288633763354767 1107 84-85 -0.5013887017705443 1107 86-87 -0.5300011384922243 1107 88-89 -0.5643442063384896 1107 90-91 -0.6480875064597669 1107 92-93 -0.5470765429234241 1107 94-95 -0.4061757233174177 1107 96-97 -0.247539508194631 1107 98-99 0.02001059425789009 1107 100-101 0.0702344480392263 1108 1 0.11837037257252092 1108 2 0.1303651811009665 1108 3 0.1365001679196176 1108 4 0.1377870576596365 1108 5 0.05179546807546842 1108 6 -0.05793781226373795 1108 7 0.07627822157863307 1108 8 0.12393656408737286 1108 9 -0.08845248866742139 1108 10-11 0.16999020986938973 1108 12-13 -0.008229489184323313 1108 14-15 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2316 54-55 -1.7449691024093994 2316 56-57 -1.9769480022199701 2316 58-59 -1.4200403164907023 2316 60-61 -1.680310249634939 2316 62-63 -1.284572203486018 2316 64-65 -1.3264251400457958 2316 66-67 -1.5464387414467033 2316 68-69 -1.7483077479159235 2316 70-71 -1.5435092255180791 2316 72-73 -1.309431918533516 2316 74-75 -1.1691114949461152 2316 76-77 -1.142865782045618 2316 78-79 -2.166637001419769 2316 80-81 -2.122692574223773 2316 82-83 -2.0875492944572898 2316 84-85 -1.7379878808691274 2316 86-87 -1.4257249525264832 2316 88-89 -2.0901591877892756 2316 90-91 -2.095230138080261 2316 92-93 -2.8458343375402073 2316 94-95 -2.7201823536749856 2316 96-97 -3.1936688396335775 2316 98-99 -3.8612961387123548 2316 100-101 -4.499435612739049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3552.0 3 1416.0 4 1511.0 5 1627.0 6 1856.0 7 2280.0 8 2833.0 9 3554.0 10 4414.0 11 5783.0 12 7211.0 13 8437.0 14 8779.0 15 9508.0 16 11241.0 17 13662.0 18 11170.0 19 9060.0 20 8933.0 21 9510.0 22 10293.0 23 11723.0 24 13142.0 25 15148.0 26 17611.0 27 20376.0 28 23845.0 29 27964.0 30 33324.0 31 40125.0 32 48927.0 33 60842.0 34 78411.0 35 102914.0 36 138770.0 37 172745.0 38 125634.0 39 16284.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.14941919180274 17.89092149981785 13.710116991694834 22.249542316684575 2 15.864770941394346 20.01385271483698 40.443840245549055 23.677536098219623 3 19.353083372761475 25.974797348584417 29.366447436986455 25.30567184166766 4 12.789857913013803 16.565009062022977 37.03881681800783 33.60631620695539 5 13.623096961780574 36.50318802718679 33.58747149593748 16.286243515095162 6 30.529863463122865 35.204445498268726 19.195614286781876 15.070076751826535 7 28.555843283939964 32.40282711581805 21.129776567843034 17.911553032398952 8 27.07146342430791 32.90590131061321 20.566730218816787 19.455905046262092 9 27.19831005663893 17.010958803018283 20.84497168053429 34.94575945980849 10-11 24.977651698609286 26.33653912682488 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21.675487830574635 36-37 22.45720277089235 25.950096641879135 29.657022766170915 21.9356778210576 38-39 21.986494137869343 26.27271033863326 29.631286090787867 22.109509432709526 40-41 22.277951759676284 26.114483163101575 29.790281112943145 21.817283964278996 42-43 22.1734946492145 26.24962708882133 29.275245106967297 22.30163315499687 44-45 22.113201109371794 26.476038537528908 29.150980152573204 22.259780200526098 46-47 22.40999475290227 26.63773539319574 28.707295863461113 22.244973990440876 48-49 22.422238821110096 26.697927905497 28.456349535692222 22.423483737700685 50-51 22.337439694496688 26.9998049566824 28.35865284548834 22.304102503332565 52-53 22.76966178415991 27.033382624104156 27.927164010083168 22.26979158165277 54-55 22.248709842820308 27.000527498146383 28.183986189139443 22.566776469893874 56-57 22.324638007255533 27.361966924071435 27.908437183584866 22.404957885088166 58-59 22.604701304965104 26.841169225860266 28.009648100697326 22.544481368477307 60-61 22.446915983601833 26.97052120109979 28.485982204733627 22.096580610564747 62-63 22.305605749098476 27.084317928539253 28.382900065334855 22.227176257027416 64-65 22.2040915317585 27.11012112110681 28.164103907145865 22.521683439988823 66-67 22.09364099366737 27.450503861741005 27.83229695653858 22.623558188053046 68-69 22.152261626182547 27.180152840069617 28.16138479542522 22.506200738322615 70-71 22.280518196001797 27.091481468154342 27.974178774114556 22.653821561729302 72-73 22.226346914381843 27.201325148304463 27.94759724640675 22.624730690906944 74-75 22.108239717475897 27.587840246770433 27.70379061779778 22.600129417955888 76-77 22.287420731004264 27.38784734232676 27.712394788423843 22.612337138245127 78-79 22.30497677602939 27.53918180023141 27.67393988156421 22.481901542174988 80-81 22.288237310989828 27.666285343004404 27.468363133954433 22.577114212051335 82-83 22.392869680503857 27.648763214696114 27.62542111500505 22.33294598979498 84-85 22.234309731485126 27.68245093490915 27.380206839367915 22.70303249423781 86-87 22.41090596327631 27.497849752462123 27.441170841490436 22.65007344277113 88-89 22.284717603989783 27.429118523034695 27.377373822715832 22.908790050259693 90-91 22.22845948727221 27.509429314848475 27.555601476586656 22.706509721292655 92-93 22.46828583472384 27.383946862804237 27.589594304040908 22.55817299843101 94-95 22.16743407476407 27.30956789198337 27.514958117271636 23.00803991598092 96-97 22.184985946085543 27.253987322654353 27.596722982242955 22.964303749017148 98-99 22.058829635049225 27.436007560027438 27.310714452117136 23.1944483528062 100-101 22.06140405518327 27.252496365211044 27.39110513733758 23.29499444226811 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 189.0 2 538.0 3 826.5 4 555.0 5 414.0 6 555.0 7 884.0 8 1358.5 9 1618.0 10 1475.0 11 1317.5 12 1407.5 13 1462.5 14 1538.5 15 1718.0 16 1824.5 17 1940.5 18 2057.0 19 2156.0 20 2334.0 21 2692.5 22 3174.0 23 3653.5 24 4286.5 25 5213.0 26 6509.5 27 7968.5 28 9262.5 29 10810.0 30 12932.0 31 15387.5 32 18093.0 33 19906.5 34 21661.0 35 23549.0 36 25252.5 37 27178.5 38 28119.0 39 28490.0 40 29364.5 41 31711.5 42 33810.0 43 34952.0 44 36263.0 45 37887.0 46 38470.0 47 38947.0 48 41817.5 49 42409.5 50 40037.0 51 37885.5 52 36536.5 53 36391.5 54 35794.5 55 33507.5 56 30528.0 57 27691.5 58 25456.0 59 22776.5 60 19188.5 61 15823.0 62 13018.0 63 10211.0 64 7534.5 65 5808.5 66 4474.0 67 3625.5 68 2852.0 69 2079.0 70 1639.0 71 1289.5 72 1085.0 73 937.0 74 679.0 75 536.5 76 385.5 77 212.0 78 138.0 79 97.5 80 51.5 81 22.5 82 18.0 83 14.0 84 10.0 85 9.5 86 8.5 87 5.0 88 2.5 89 3.0 90 3.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014201152875410704 2 0.014293368153822462 3 0.015492166773175314 4 0.020656222364233753 5 0.03347414606346809 6 0.049427389228702184 7 0.005809562539940742 8 0.024898125171174613 9 0.033105284949821054 10-11 0.04094358361482047 12-13 0.03259810091855639 14-15 0.02780290644114498 16-17 0.029462781452556625 18-19 0.02494423281038049 20-21 0.05380761495326068 22-23 0.01387839940096955 24-25 0.002904781269970371 26-27 0.0012910138977646095 28-29 1.8443055682351565E-4 30-31 0.003227534744411524 32-33 0.002628135434735098 34-35 4.610763920587891E-5 36-37 2.766458352352734E-4 38-39 9.221527841175782E-5 40-41 0.0013832291761763673 42-43 0.004195795167734981 44-45 0.001982628485852793 46-47 9.221527841175782E-4 48-49 8.299375057058203E-4 50-51 0.004103579889323223 52-53 0.0034580729404409183 54-55 0.00507184031264668 56-57 4.610763920587891E-4 58-59 0.0055790243439113475 60-61 0.0027203507131468557 62-63 5.07184031264668E-4 64-65 7.838298664999415E-4 66-67 0.01779754873346926 68-69 0.043848364884790846 70-71 0.053669292035643044 72-73 0.015031090381116524 74-75 0.03084601062873299 76-77 0.027111291853056795 78-79 0.057634549007348636 80-81 0.084146441550729 82-83 0.049842357981555105 84-85 0.018074194568704533 86-87 0.02226998973643951 88-89 0.023053819602939455 90-91 0.03988310791308526 92-93 0.026004708512115705 94-95 0.013048461895263732 96-97 0.018581378599969202 98-99 0.05168666354979026 100-101 0.07317282341972982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1084419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.49535722306673 #Duplication Level Percentage of deduplicated Percentage of total 1 86.22457774008588 57.33534098235098 2 8.323388286148612 11.069333547874823 3 2.1316411900018846 4.252327272017351 4 0.9553765560579506 2.5411242151046665 5 0.5410908250089134 1.799001384954579 6 0.3469271469067223 1.3841426738365108 7 0.2298404026949587 1.0698323777046352 8 0.17991483666456695 0.9570801066992066 9 0.13658513206483014 0.8174049433209561 >10 0.8334670736504217 10.519825302585575 >50 0.06699071505784196 3.0472385807958084 >100 0.028390874562856706 3.482547906742756 >500 9.741959739532975E-4 0.3901577302579201 >1k 8.350251205313979E-4 1.334642975754204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4762 0.43912915579679074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3704 0.3415653912371509 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2239 0.20647000836392573 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1542 0.14219595931093057 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1099 0.10134459097452184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 9.221527841175781E-5 0.0 0.0 0.0 0.0 9 9.221527841175781E-5 0.0 0.0 0.0 0.0 10-11 9.221527841175781E-5 0.0 0.0 0.0 0.0 12-13 9.221527841175781E-5 0.0 0.0 0.0 0.0 14-15 1.8443055682351562E-4 0.0 0.0 0.0 0.0 16-17 2.766458352352735E-4 0.0 0.0 0.0 0.0 18-19 3.2275347444115233E-4 0.0 0.0 0.0 9.221527841175781E-5 20-21 3.6886111364703124E-4 0.0 0.0 2.3053819602939456E-4 9.221527841175781E-5 22-23 3.6886111364703124E-4 0.0 0.0 4.6107639205878907E-4 9.221527841175781E-5 24-25 3.6886111364703124E-4 0.0 0.0 6.916145880881836E-4 9.221527841175781E-5 26-27 3.6886111364703124E-4 0.0 0.0 7.377222272940625E-4 9.221527841175781E-5 28-29 3.6886111364703124E-4 0.0 0.0 0.0013371215369704884 9.221527841175781E-5 30-31 3.6886111364703124E-4 0.0 0.0 0.0028125659915586134 9.221527841175781E-5 32-33 3.6886111364703124E-4 0.0 0.0 0.00424190280694086 9.221527841175781E-5 34-35 3.6886111364703124E-4 4.6107639205878905E-5 0.0 0.006270638931999532 9.221527841175781E-5 36-37 3.6886111364703124E-4 9.221527841175781E-5 0.0 0.00977481951164633 9.221527841175781E-5 38-39 5.53291670470547E-4 9.221527841175781E-5 0.0 0.014293368153822462 9.221527841175781E-5 40-41 5.993993096764258E-4 9.221527841175781E-5 0.0 0.0203334688897926 9.221527841175781E-5 42-43 6.455069488823048E-4 9.221527841175781E-5 0.0 0.025543632120056916 9.221527841175781E-5 44-45 6.455069488823048E-4 9.221527841175781E-5 0.0 0.028079552276380256 9.221527841175781E-5 46-47 6.455069488823048E-4 9.221527841175781E-5 0.0 0.030569364793497717 9.221527841175781E-5 48-49 6.916145880881836E-4 9.221527841175781E-5 0.0 0.035410666910115005 9.221527841175781E-5 50-51 7.377222272940625E-4 9.221527841175781E-5 0.0 0.04122022945005575 9.221527841175781E-5 52-53 7.838298664999415E-4 9.221527841175781E-5 0.0 0.05242438577708432 9.221527841175781E-5 54-55 8.299375057058203E-4 1.383229176176367E-4 0.0 0.06289081987681883 9.221527841175781E-5 56-57 9.221527841175781E-4 1.8443055682351562E-4 0.0 0.08534524017008185 9.221527841175781E-5 58-59 9.221527841175781E-4 1.8443055682351562E-4 0.0 0.1200181848529028 9.221527841175781E-5 60-61 9.221527841175781E-4 1.8443055682351562E-4 0.0 0.15031090381116524 9.221527841175781E-5 62-63 9.221527841175781E-4 1.8443055682351562E-4 0.0 0.16838509837986976 9.221527841175781E-5 64-65 9.221527841175781E-4 1.8443055682351562E-4 0.0 0.20033769234954385 9.221527841175781E-5 66-67 0.0011987986193528516 1.8443055682351562E-4 0.0 0.25829499483133367 9.221527841175781E-5 68-69 0.0011987986193528516 1.8443055682351562E-4 0.0 0.34216479054682736 9.221527841175781E-5 70-71 0.0012910138977646095 1.8443055682351562E-4 0.0 0.4206861001144392 9.221527841175781E-5 72-73 0.0013371215369704884 1.8443055682351562E-4 0.0 0.5067229548726092 1.383229176176367E-4 74-75 0.0013832291761763673 1.8443055682351562E-4 0.0 0.6081597611255428 1.8443055682351562E-4 76-77 0.0013832291761763673 1.8443055682351562E-4 0.0 0.6841451505368312 1.8443055682351562E-4 78-79 0.0014293368153822461 1.8443055682351562E-4 0.0 0.772948463647354 1.8443055682351562E-4 80-81 0.0016598750114116406 1.8443055682351562E-4 0.0 0.89707022838958 1.8443055682351562E-4 82-83 0.0016598750114116406 1.8443055682351562E-4 0.0 1.0075902395660719 1.8443055682351562E-4 84-85 0.0016598750114116406 1.8443055682351562E-4 0.0 1.15674845239709 1.8443055682351562E-4 86-87 0.0017059826506175195 1.8443055682351562E-4 0.0 1.3701346066418978 1.8443055682351562E-4 88-89 0.0018443055682351563 1.8443055682351562E-4 0.0 1.6347002404052309 1.8443055682351562E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1705 0.0 52.383125 2 GTACCTG 1750 0.0 51.84814 1 ACCTGGG 2300 0.0 35.940105 3 GTACACG 235 0.0 34.365208 1 GGTATCA 3890 0.0 32.60615 1 GTATCAA 5800 0.0 30.304815 1 CGTATCC 70 0.008383415 27.14813 8 GTACATG 4370 0.0 26.850565 1 TCACGCA 160 6.2322215E-8 26.722706 4 TATCACG 160 6.2322215E-8 26.722706 2 CCTGGGG 1570 0.0 26.022963 4 ATCACGC 165 8.397183E-8 25.912928 3 TACATGG 4430 0.0 25.630205 2 ACATGGG 4470 0.0 24.656897 3 ATCAACG 7080 0.0 24.558722 3 CAACGCA 7170 0.0 24.250454 5 TATCAAC 7165 0.0 24.20107 2 TCAACGC 7195 0.0 24.100164 4 TTCAACG 220 2.1645974E-9 23.75352 2 AACGCAG 7370 0.0 23.656828 6 >>END_MODULE