FastQCFastQC Report
Thu 26 May 2016
SRR936723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084419
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55110.5081983993271973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48430.44659859334814317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30190.27839792552509685No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.20204367500016138No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.12947025089010797No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12630.11646789663405013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG16350.053.752261
TACCTGG16300.052.459922
ATAACGC1101.6189006E-938.866243
GGTATCA48900.033.2246171
ACCTGGG25550.032.164583
TATAACG1351.1958036E-831.670252
GTATCAA72000.029.2291
TATCACG1004.949038E-528.5032222
ATCACGC1004.9505943E-528.5019073
TAACGCA1554.586036E-827.5812234
GTACATG46750.024.0829371
CCTGGGG16800.024.033244
TATCAAC88400.023.537732
ATCAACG88700.023.4034843
TCAACGC89700.023.141514
CAACGCA91700.022.7403895
TACATGG49100.022.6400342
AACGCAG93350.022.3882946
ACATGGG48300.021.9321123
GAGTACT62800.021.74507312-13
Produced by FastQC (version 0.11.5)