FastQCFastQC Report
Thu 26 May 2016
SRR936722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112260
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.4471076906478701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.3433549709600273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.2219804721917537No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.14618884073867622No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.10195457896534983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15600.047.8306162
GTACCTG16500.046.0899281
GTACACG2800.039.0426941
TAACGCA1456.6029315E-1032.7750474
GGTATCA44800.032.1465641
ATAACGC1509.495125E-1031.6825493
ACCTGGG23350.031.3433343
GTATCAA67250.030.5324361
TATAACG1554.562935E-827.5957182
GTACATG49600.027.406551
TACATGG51550.025.3533252
ATCAACG81300.025.252513
TCAACGC82000.025.0948984
TATCAAC82250.024.9041822
CAACGCA82800.024.564355
ACATGGG51300.024.4567033
AACGCAG84700.024.068346
TACACGG4100.023.1833932
GAGTACT50600.022.53536812-13
ACGCAGA93850.021.617597
Produced by FastQC (version 0.11.5)