##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936722_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1112260 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.013138115188895 31.0 31.0 34.0 28.0 34.0 2 31.015551220038482 31.0 31.0 34.0 27.0 34.0 3 31.052952547066333 31.0 31.0 34.0 27.0 34.0 4 34.708823476525275 37.0 35.0 37.0 32.0 37.0 5 34.6365535036772 37.0 35.0 37.0 32.0 37.0 6 34.749396723787605 37.0 35.0 37.0 32.0 37.0 7 34.5821570496107 37.0 35.0 37.0 30.0 37.0 8 34.55901407944186 37.0 35.0 37.0 30.0 37.0 9 36.00845126139572 39.0 35.0 39.0 30.0 39.0 10-11 35.850988527862185 38.5 35.0 39.0 30.0 39.0 12-13 35.74173709384496 38.0 35.0 39.0 30.0 39.0 14-15 36.72489615737328 39.0 36.0 41.0 30.0 41.0 16-17 36.64931940373654 39.0 36.0 41.0 30.0 41.0 18-19 36.61225298041825 39.0 36.0 41.0 30.0 41.0 20-21 36.51489130239332 39.0 36.0 41.0 29.5 41.0 22-23 36.37721171308867 39.0 35.5 40.5 29.0 41.0 24-25 36.20754275079568 39.0 35.0 40.5 29.0 41.0 26-27 35.98308668836423 38.5 35.0 40.0 27.5 41.0 28-29 36.053539640012225 39.0 35.0 40.0 28.0 41.0 30-31 35.93885782101307 38.5 35.0 40.0 27.5 41.0 32-33 35.73795650297592 38.0 35.0 40.0 27.0 41.0 34-35 35.52153543236294 38.0 34.5 40.0 27.0 41.0 36-37 35.23717206408573 38.0 34.0 40.0 25.5 41.0 38-39 35.029734504522324 38.0 34.0 40.0 25.0 41.0 40-41 34.84902765540431 38.0 34.0 40.0 25.0 41.0 42-43 34.561317048172185 38.0 33.0 40.0 24.0 41.0 44-45 34.252578533796054 38.0 33.0 40.0 23.5 41.0 46-47 33.87121266610325 38.0 33.0 40.0 22.0 41.0 48-49 33.56829473324582 38.0 33.0 40.0 19.5 41.0 50-51 32.843445327531334 37.0 32.0 39.5 18.0 40.5 52-53 33.011991800478306 37.5 32.5 39.5 18.0 40.5 54-55 33.3571732328772 38.0 33.0 40.0 18.0 41.0 56-57 33.27196788520669 37.5 33.0 40.0 17.5 41.0 58-59 33.02692850592487 37.0 32.0 40.0 14.5 41.0 60-61 32.70651061802097 37.0 32.0 40.0 13.0 41.0 62-63 32.373534515311164 36.0 31.0 39.5 11.0 41.0 64-65 32.059288295902036 36.0 31.0 39.0 8.0 41.0 66-67 31.69273596101631 35.0 31.0 39.0 7.5 40.5 68-69 31.308024652509303 35.0 30.5 38.5 7.0 40.0 70-71 30.909034308524987 35.0 30.0 38.0 3.5 40.0 72-73 30.493128854764173 35.0 29.5 37.0 2.0 39.5 74-75 30.065494578605723 34.5 29.0 37.0 2.0 39.0 76-77 29.651331972740188 34.0 29.0 36.0 2.0 39.0 78-79 29.19653812957402 34.0 29.0 36.0 2.0 38.0 80-81 28.79157436210958 34.0 28.0 35.0 2.0 37.0 82-83 28.368051534713107 34.0 27.0 35.0 2.0 37.0 84-85 27.979855429485912 34.0 26.5 35.0 2.0 36.0 86-87 27.626259597576105 34.0 26.0 35.0 2.0 36.0 88-89 27.256044450038658 33.0 26.0 35.0 2.0 35.5 90-91 26.89730368798662 33.0 25.0 35.0 2.0 35.0 92-93 26.511261305809793 33.0 25.0 35.0 2.0 35.0 94-95 26.06126759930232 33.0 24.0 35.0 2.0 35.0 96-97 25.460571269307536 32.0 20.5 35.0 2.0 35.0 98-99 24.77277030550411 32.0 10.0 35.0 2.0 35.0 100-101 22.711625429306096 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43276602772304784 1101 2 0.7491468363821596 1101 3 0.7359330658199745 1101 4 0.7742055057096877 1101 5 0.88473869057038 1101 6 0.6812786444632053 1101 7 0.5587776704960703 1101 8 0.6001979149570786 1101 9 0.8353950635828724 1101 10-11 0.6295500033139376 1101 12-13 0.7467856212479944 1101 14-15 0.807471045004732 1101 16-17 1.0501404832803019 1101 18-19 1.0313558219096919 1101 20-21 1.137536675302762 1101 22-23 1.0762142634893053 1101 24-25 1.1406800426874852 1101 26-27 1.0718518134116053 1101 28-29 1.067743259775085 1101 30-31 1.0005618142388641 1101 32-33 0.8028922364379767 1101 34-35 0.6436280039632223 1101 36-37 1.0005383898825428 1101 38-39 0.8600107640437571 1101 40-41 0.7205122999527163 1101 42-43 0.6684230135818012 1101 44-45 0.7322945123178926 1101 46-47 0.6489203140444246 1101 48-49 0.7850099353362978 1101 50-51 0.7396236093292998 1101 52-53 0.667971848531522 1101 54-55 0.943761949765495 1101 56-57 0.7645641395573577 1101 58-59 0.9094167558070723 1101 60-61 0.9356798982697185 1101 62-63 1.0588223857585959 1101 64-65 1.000513003002979 1101 66-67 0.996278446824622 1101 68-69 0.9177971003375482 1101 70-71 0.9091625897584485 1101 72-73 0.8117411260278793 1101 74-75 0.8213657451298495 1101 76-77 0.9944067147213751 1101 78-79 0.9463173429887846 1101 80-81 0.9144164849693865 1101 82-83 0.9879391049067365 1101 84-85 0.9678586351242124 1101 86-87 1.0762100114882678 1101 88-89 1.034826349630574 1101 90-91 0.9216559262659096 1101 92-93 0.6702831602068535 1101 94-95 0.4447338859633909 1101 96-97 0.5110724590940698 1101 98-99 0.6189000664643061 1101 100-101 0.8868145107358103 1102 1 0.17335527195327316 1102 2 0.25937212713242985 1102 3 0.13622833927358613 1102 4 0.448430593821314 1102 5 0.3650581359194618 1102 6 0.5634377592218129 1102 7 0.14500403233631687 1102 8 0.1405172155642731 1102 9 0.26160203000999616 1102 10-11 0.41044552138134804 1102 12-13 0.7016326652567741 1102 14-15 0.49908868035648624 1102 16-17 0.31946243990115164 1102 18-19 0.39825986527436186 1102 20-21 0.33317668775055864 1102 22-23 0.3821323974768731 1102 24-25 0.5909221234968882 1102 26-27 0.44577149613738243 1102 28-29 0.6064063513170908 1102 30-31 0.523510278889276 1102 32-33 0.3793800021298068 1102 34-35 0.3853169246953243 1102 36-37 0.1616596329258897 1102 38-39 0.182873107270197 1102 40-41 -0.05570479840663012 1102 42-43 0.17669211770187587 1102 44-45 0.14743234278171258 1102 46-47 0.44078473431697063 1102 48-49 0.4051927443421448 1102 50-51 0.4356725176136038 1102 52-53 0.3089414020751633 1102 54-55 0.20953881566936872 1102 56-57 0.5959452666624472 1102 58-59 0.4248853214873165 1102 60-61 0.13488345602535645 1102 62-63 0.3139310507317816 1102 64-65 0.3263345941505662 1102 66-67 0.22781046734902333 1102 68-69 0.14196718653134255 1102 70-71 0.16292443145424684 1102 72-73 0.26101850214691424 1102 74-75 0.203186525722856 1102 76-77 0.33295292718799274 1102 78-79 0.25415403071539444 1102 80-81 0.24447792790127565 1102 82-83 0.20092209049768073 1102 84-85 0.05498927191615621 1102 86-87 -0.24593694495166218 1102 88-89 0.011078547898016211 1102 90-91 -0.30271969341030314 1102 92-93 -0.4159663603356414 1102 94-95 -0.21796576737978413 1102 96-97 -0.29604630886296945 1102 98-99 -0.3919425484426604 1102 100-101 -0.2006544602217275 1103 1 0.3579706565686571 1103 2 0.5027960332429053 1103 3 0.3479007464683832 1103 4 0.2999039960409817 1103 5 0.37453638569621717 1103 6 0.14365207227194787 1103 7 0.3274252595336691 1103 8 0.1883677066983438 1103 9 0.4616913271142167 1103 10-11 0.4885358390239034 1103 12-13 0.5443677079918174 1103 14-15 0.7533239144424684 1103 16-17 0.8170131478996225 1103 18-19 0.6376845367885906 1103 20-21 0.6598892786422539 1103 22-23 0.6665500527900505 1103 24-25 0.5664993654920423 1103 26-27 0.6220439798725508 1103 28-29 0.6708342120136095 1103 30-31 0.547920280164945 1103 32-33 0.5943691589100055 1103 34-35 0.3597141692418262 1103 36-37 0.5597652586434521 1103 38-39 0.6141398504772368 1103 40-41 0.547426978605742 1103 42-43 0.5801619480373787 1103 44-45 0.656553404141583 1103 46-47 0.44704828834171906 1103 48-49 0.19362239098074951 1103 50-51 -0.08488495145132902 1103 52-53 0.3464461858486061 1103 54-55 0.5688958765189653 1103 56-57 0.5619485834120326 1103 58-59 0.5712624104017223 1103 60-61 0.7509441780562227 1103 62-63 0.5828365233685986 1103 64-65 0.5391997555231853 1103 66-67 0.6196908092293647 1103 68-69 0.35598683189427405 1103 70-71 0.53512114021812 1103 72-73 0.5463737770544306 1103 74-75 0.36367255710440816 1103 76-77 0.2991348381460206 1103 78-79 0.3512454964635765 1103 80-81 0.5671530140092003 1103 82-83 0.7427067569092145 1103 84-85 0.5075788879386067 1103 86-87 0.38955227561218564 1103 88-89 0.307487528635356 1103 90-91 0.45293791597864796 1103 92-93 0.3103252584932896 1103 94-95 0.39400004458602567 1103 96-97 0.6714240317417008 1103 98-99 0.6884120886283931 1103 100-101 0.5456014397655125 1104 1 0.2686406317547636 1104 2 0.3328764006367031 1104 3 0.2708850803819409 1104 4 0.4513531193244802 1104 5 0.30520150509508426 1104 6 0.36572615183278856 1104 7 0.3542678867614626 1104 8 0.42733827980469385 1104 9 0.5003668494199758 1104 10-11 0.5932963622967051 1104 12-13 0.43298088692757375 1104 14-15 0.4032381151510833 1104 16-17 0.4991977583461491 1104 18-19 0.5681799093879576 1104 20-21 0.5831215457599299 1104 22-23 0.42587378154083666 1104 24-25 0.41540516733476807 1104 26-27 0.24516493424049912 1104 28-29 0.21507742934301177 1104 30-31 0.5075328871054339 1104 32-33 0.27644369112898204 1104 34-35 0.3605443854066621 1104 36-37 0.6778575926722326 1104 38-39 0.4013318886322068 1104 40-41 0.41378789527961146 1104 42-43 0.5343982108918368 1104 44-45 0.370619549839887 1104 46-47 0.3073549025000446 1104 48-49 0.3088321157234546 1104 50-51 0.20160855290447444 1104 52-53 0.2985333513445312 1104 54-55 0.4824227417791036 1104 56-57 0.34580741168588247 1104 58-59 0.43221920624056054 1104 60-61 0.2479658933013411 1104 62-63 0.19809151392450275 1104 64-65 0.1760588841974311 1104 66-67 -0.0029311924248922594 1104 68-69 0.13572235609296612 1104 70-71 0.2942864386227022 1104 72-73 -0.07970385793028001 1104 74-75 -0.053733794100690346 1104 76-77 -0.08648462450762295 1104 78-79 0.09563459316369816 1104 80-81 0.2206565879509057 1104 82-83 -0.16228441625721146 1104 84-85 0.07802483850010589 1104 86-87 0.08196490230502462 1104 88-89 0.2218312360700594 1104 90-91 0.06343690984307315 1104 92-93 -0.08161290017573108 1104 94-95 -0.25839863200619817 1104 96-97 -0.3151392231171748 1104 98-99 0.03518731094526473 1104 100-101 0.2080028323065335 1105 1 0.12080684238772932 1105 2 0.1906282657235323 1105 3 0.10031836497730495 1105 4 0.3265657159662112 1105 5 0.2907702124872813 1105 6 0.29313286715652964 1105 7 0.39765276909263747 1105 8 -0.04520363788274295 1105 9 0.5458893265222287 1105 10-11 0.4666029200815345 1105 12-13 0.20804605626673833 1105 14-15 0.3814607364449216 1105 16-17 0.3527330812402525 1105 18-19 0.3481835900147132 1105 20-21 -0.10432043886637388 1105 22-23 0.017533521753300363 1105 24-25 0.009278339408112402 1105 26-27 -0.044237489715122535 1105 28-29 0.23638461809242983 1105 30-31 0.09453926348793829 1105 32-33 -0.1383963128347787 1105 34-35 0.1726938916566425 1105 36-37 0.27404025692020895 1105 38-39 0.10273497590957703 1105 40-41 0.1468666182234699 1105 42-43 0.22947041791293543 1105 44-45 0.2129036820916923 1105 46-47 0.10619651623242277 1105 48-49 0.4112425217855602 1105 50-51 0.24730292741026716 1105 52-53 0.30978251966444503 1105 54-55 0.19658769452753688 1105 56-57 0.5116611047499262 1105 58-59 0.2752374415215897 1105 60-61 0.34083397114457625 1105 62-63 0.2840614500797827 1105 64-65 0.2036160603073398 1105 66-67 -0.014987267380252689 1105 68-69 0.15867773759397252 1105 70-71 0.4172042049271738 1105 72-73 0.3577062751183071 1105 74-75 0.2947972920279973 1105 76-77 0.3843039396636989 1105 78-79 0.17093981217110255 1105 80-81 0.38671352441560103 1105 82-83 0.35155736937256776 1105 84-85 0.15402068060526375 1105 86-87 0.2558017130217145 1105 88-89 0.4705607120256552 1105 90-91 0.6152467872701131 1105 92-93 0.5764437288959634 1105 94-95 0.5123782026952384 1105 96-97 0.6426670424420671 1105 98-99 0.2758274973224957 1105 100-101 0.37641071855494346 1106 1 -0.13588683728883666 1106 2 0.13738720514750824 1106 3 0.013290016335265165 1106 4 -0.024002678611964257 1106 5 0.1901552610165851 1106 6 0.2742317700158239 1106 7 0.30798519531748525 1106 8 0.29121546626252126 1106 9 0.2952650636730283 1106 10-11 0.27049928143510016 1106 12-13 0.20543786906197425 1106 14-15 0.26784589911370915 1106 16-17 0.1505970710357829 1106 18-19 -0.01969095267645571 1106 20-21 0.1500113545852244 1106 22-23 0.1412527065971787 1106 24-25 0.0494113819861397 1106 26-27 -0.10091575054986635 1106 28-29 -0.2194604245496805 1106 30-31 -0.18888708350808514 1106 32-33 -0.29003661728680896 1106 34-35 -0.08130249192408456 1106 36-37 -0.1553304340641759 1106 38-39 -0.02257114817405892 1106 40-41 -0.16006545276728446 1106 42-43 -0.08088783987808057 1106 44-45 -0.1292534339040614 1106 46-47 0.013840657372895748 1106 48-49 -0.37030653115713363 1106 50-51 0.10336503530611907 1106 52-53 -0.10616076302700606 1106 54-55 0.2750956312261863 1106 56-57 0.18678610750328772 1106 58-59 0.020471117073121547 1106 60-61 0.1280909992329029 1106 62-63 0.10471729151802123 1106 64-65 0.05010346791944187 1106 66-67 0.10924854878710377 1106 68-69 0.058281052845213566 1106 70-71 0.17939159792141268 1106 72-73 0.33086247199088703 1106 74-75 0.22098509352142415 1106 76-77 0.16476165899672424 1106 78-79 0.4702725968339614 1106 80-81 0.18871657213992066 1106 82-83 0.23150967108525933 1106 84-85 0.3932019601288417 1106 86-87 0.3061242071094874 1106 88-89 0.5021703389898065 1106 90-91 0.4103223696317606 1106 92-93 0.25851021414093367 1106 94-95 0.22671912730511323 1106 96-97 0.2920015291848692 1106 98-99 0.5915703350702195 1106 100-101 0.9262045666372991 1107 1 0.32288293727041406 1107 2 0.40588584733036015 1107 3 0.4307627522290538 1107 4 0.37951471437911266 1107 5 0.07117599466889857 1107 6 0.34038332827264384 1107 7 0.5818461375994772 1107 8 0.4356139317136183 1107 9 0.4926636584400441 1107 10-11 0.44913198112043773 1107 12-13 0.5019700471730957 1107 14-15 0.6161152210672398 1107 16-17 0.5474988771481222 1107 18-19 0.7588986417026149 1107 20-21 0.7229315235580245 1107 22-23 0.5196267744449372 1107 24-25 0.6093094379368367 1107 26-27 0.6810166603299166 1107 28-29 0.5618157080422321 1107 30-31 0.4384675438465422 1107 32-33 0.7125109063133408 1107 34-35 0.4306947924416207 1107 36-37 0.6584320125930034 1107 38-39 0.5604259637704203 1107 40-41 0.5154378016833334 1107 42-43 0.712375878385636 1107 44-45 0.8554732784542267 1107 46-47 0.9124558994091885 1107 48-49 1.0735800587820918 1107 50-51 0.8649797600787394 1107 52-53 0.8559949333392183 1107 54-55 1.077678716704007 1107 56-57 1.1864423426858366 1107 58-59 1.1710616597689238 1107 60-61 1.0774498078022319 1107 62-63 1.1130538577493283 1107 64-65 0.7789549270340501 1107 66-67 0.8648662478258551 1107 68-69 1.0407188734409267 1107 70-71 1.205052186739895 1107 72-73 1.2112096852328378 1107 74-75 0.8138253021511055 1107 76-77 0.9633510378492609 1107 78-79 1.015051466612828 1107 80-81 1.1992687511131557 1107 82-83 1.2619657252255259 1107 84-85 1.282823280013318 1107 86-87 1.2296478503699753 1107 88-89 1.27703671254039 1107 90-91 1.1441201626400783 1107 92-93 0.8982824025973315 1107 94-95 1.126456184936906 1107 96-97 0.8171340780016294 1107 98-99 1.199791594607273 1107 100-101 1.3174854408129093 1108 1 -0.027044383081328505 1108 2 -0.019114512469876388 1108 3 -0.18271320591898288 1108 4 -0.14271950118440202 1108 5 0.09131617284197802 1108 6 -0.0531381086255891 1108 7 0.0960568078353603 1108 8 -0.06678665548037088 1108 9 0.21855848163420433 1108 10-11 0.1943113775195613 1108 12-13 0.21796172859157537 1108 14-15 0.33951518940482117 1108 16-17 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-2.0680818727884294 2316 76-77 -2.684283228341382 2316 78-79 -2.64025487912021 2316 80-81 -2.848623682377575 2316 82-83 -2.5520693624937714 2316 84-85 -2.6614193012757887 2316 86-87 -2.9412445063423753 2316 88-89 -3.2202040644568086 2316 90-91 -2.833436557387561 2316 92-93 -3.553954727426852 2316 94-95 -3.6030823770040534 2316 96-97 -3.8703510461873982 2316 98-99 -4.150159459175985 2316 100-101 -4.803500969413967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3638.0 3 1553.0 4 1557.0 5 1719.0 6 1830.0 7 2333.0 8 2908.0 9 3611.0 10 4645.0 11 5653.0 12 7269.0 13 8306.0 14 8621.0 15 9601.0 16 11281.0 17 13210.0 18 11461.0 19 8593.0 20 8788.0 21 9512.0 22 10524.0 23 11811.0 24 13531.0 25 15399.0 26 17773.0 27 21040.0 28 24413.0 29 28828.0 30 34248.0 31 41281.0 32 50543.0 33 62331.0 34 79946.0 35 104796.0 36 140607.0 37 175997.0 38 133648.0 39 19450.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.19223475396746 17.767862682524825 13.817800233610194 22.22210232989752 2 15.803287001696638 20.10197815319021 40.27948235882248 23.81525248629067 3 19.334665871819148 25.916614740824862 29.36089404161081 25.387825345745185 4 12.847415524391373 16.56820060275801 37.00702138432883 33.577362488521786 5 13.664308554415728 36.52669404055713 33.50168425881368 16.307313146213453 6 30.502092012569165 35.232352382897226 19.16258324644727 15.102972358086342 7 28.642988314306834 32.38728441182131 21.10250567017819 17.867221603693665 8 26.959733378417756 32.97040975262323 20.603549678593655 19.466307190365363 9 27.17148519552269 17.03135340833885 20.831834661755817 34.96532673438264 10-11 25.015470937125034 26.367603857660182 27.40042383171938 21.216501373495404 12-13 26.209860025858678 23.904480296812636 27.912575186913262 21.973084490415427 14-15 23.00906521541689 25.13472829082787 26.89004031282682 24.966166180928425 16-17 22.044355076220533 28.267626855491013 27.3675397604414 22.320478307847054 18-19 21.74070623277766 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29.25784804163085 22.349926095884744 44-45 22.187058186240023 26.43853998323215 29.190760150056526 22.183641680471297 46-47 22.474626574474044 26.63884884195043 28.614462841311177 22.27206174226435 48-49 22.453439184359702 26.812286750790058 28.365123105762617 22.36915095908762 50-51 22.36648033140255 27.040236279408493 28.281152716246684 22.312130672942278 52-53 22.665667491271076 27.086029630914975 27.93395013083833 22.31435274697561 54-55 22.234994297520625 27.01370348276062 28.154019539926573 22.597282679792187 56-57 22.437359320612117 27.366526813317133 27.82964774128524 22.36646612478551 58-59 22.59535828389479 26.969685935448258 27.965919210745525 22.469036569911427 60-61 22.502624179311802 26.996567775752357 28.429201103166772 22.07160694176907 62-63 22.348438361854463 27.065877092782127 28.395349767654714 22.1903347777087 64-65 22.22810633663678 27.111301920254267 28.186941012252493 22.473650730856463 66-67 22.158946339674007 27.359861252592747 27.86744192110135 22.613750486631893 68-69 22.17225043001017 27.22452150410499 28.09964727027836 22.503580795606478 70-71 22.279703994101464 27.16074415091625 27.91105436365925 22.648497491323035 72-73 22.298956401179996 27.258006363911818 27.85582565866667 22.58721157624152 74-75 22.15930521158742 27.66925892143081 27.595083182471868 22.576352684509903 76-77 22.266547626433045 27.414138635119205 27.716369560585097 22.602944177862653 78-79 22.351816959487717 27.514131407474697 27.626912855196355 22.507138777841234 80-81 22.31092590003008 27.78239656404268 27.397595962965976 22.50908157296126 82-83 22.449647503301986 27.733951082934954 27.47442252310492 22.34197889065814 84-85 22.23938095488572 27.750031582558154 27.362543940217478 22.648043522338646 86-87 22.4502787554662 27.45362579916569 27.48819768103146 22.607897764336656 88-89 22.307773920533577 27.522651194531882 27.339679354212805 22.829895530721735 90-91 22.338081448541555 27.52967178614284 27.494379006685847 22.63786775862976 92-93 22.45766502939226 27.450505232159518 27.507918488462273 22.58391124998595 94-95 22.158983626898284 27.32141091000638 27.611289437955744 22.908316025139587 96-97 22.156452860045675 27.304939670209134 27.63419107730485 22.904416392440343 98-99 21.961690454447556 27.486138969560365 27.325575382827928 23.226595193164155 100-101 21.97725399228908 27.34485849292071 27.583615570973002 23.09427194381721 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 251.0 1 213.0 2 614.0 3 863.0 4 525.5 5 418.0 6 567.0 7 945.5 8 1480.0 9 1708.0 10 1546.5 11 1388.0 12 1421.0 13 1545.5 14 1667.0 15 1769.5 16 1853.0 17 1908.5 18 2011.5 19 2215.5 20 2443.5 21 2823.0 22 3272.5 23 3773.5 24 4433.5 25 5302.5 26 6660.5 27 8241.5 28 9607.0 29 11082.5 30 13033.0 31 15746.5 32 18466.5 33 20199.0 34 22123.0 35 23890.5 36 25762.0 37 27955.5 38 28877.5 39 29228.0 40 30001.5 41 32288.5 42 34600.5 43 36119.0 44 37462.0 45 39001.0 46 39497.0 47 39857.5 48 42604.0 49 43022.5 50 40992.5 51 39210.0 52 37477.5 53 37210.5 54 36791.0 55 34403.5 56 31399.0 57 28328.5 58 25994.5 59 23388.5 60 19696.5 61 16323.0 62 13605.0 63 10549.5 64 7763.5 65 6077.5 66 4646.0 67 3733.5 68 2953.0 69 2193.5 70 1697.0 71 1297.5 72 1076.5 73 931.5 74 714.0 75 564.5 76 393.5 77 236.0 78 159.5 79 99.0 80 64.5 81 39.0 82 22.5 83 18.5 84 13.5 85 8.0 86 5.0 87 5.0 88 5.0 89 2.5 90 1.0 91 1.5 92 1.5 93 2.0 94 1.5 95 0.5 96 0.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013575962454821715 2 0.005484329203603474 3 0.00872098250409077 4 0.03335551040224408 5 0.04450398288170032 6 0.05852948051714527 7 0.02715192490964343 8 0.024454713825904015 9 0.014744753924442126 10-11 0.044773703990074264 12-13 0.04023339866577958 14-15 0.01861075647780195 16-17 0.023061154765971984 18-19 0.02984913599338284 20-21 0.023151061802096633 22-23 0.014654846888317481 24-25 0.0035063744088612374 26-27 0.00265225756567709 28-29 0.001528419614119001 30-31 0.007597144552532682 32-33 0.0014385125779943538 34-35 0.0017981407224929421 36-37 0.006428353082912269 38-39 0.005394422167478827 40-41 0.004090770143671443 42-43 7.192562889971769E-4 44-45 0.001123837951558089 46-47 0.0021128153489292072 48-49 4.4953518062323554E-4 50-51 0.0015733731321813244 52-53 8.541168431841475E-4 54-55 0.0022027223850538545 56-57 0.0021128153489292072 58-59 0.0018430942405552658 60-61 6.743027709348534E-4 62-63 0.0013036520238073831 64-65 0.0033715138546742667 66-67 0.002067861830866884 68-69 0.006922841781597827 70-71 0.010788844334957654 72-73 0.004585258842357003 74-75 0.0032816068185496194 76-77 0.002787118119864061 78-79 0.03371513854674267 80-81 0.022971247729847336 82-83 0.004585258842357003 84-85 0.0085861219499038 86-87 0.007597144552532682 88-89 0.006023771420351357 90-91 0.012092496358765036 92-93 0.013261287828385449 94-95 0.006293492528725297 96-97 0.0035962814449858843 98-99 0.021532735151852982 100-101 0.041402190135399995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1112260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.45944287280065 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24795315925236 57.31990915883319 2 8.37239828663681 11.128498512781464 3 2.0962646220579693 4.1794973668780395 4 0.932118264925881 2.4779224231414263 5 0.5265577420013924 1.749736708688622 6 0.3381205943589741 1.3482783794950586 7 0.2302000083855923 1.0709275014634352 8 0.18296446421877363 0.9727773086000133 9 0.13364201315615482 0.799359636488179 >10 0.8385901767945193 10.486458553420526 >50 0.07078504556315462 3.1952110166316063 >100 0.028776751872971948 3.576889924983399 >500 8.144353877073636E-4 0.32990359033922656 >1k 8.144353877073636E-4 1.3646299182558053 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4973 0.4471076906478701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3819 0.3433549709600273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 0.2219804721917537 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1626 0.14618884073867622 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1134 0.10195457896534983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 3.5962814449858846E-4 0.0 28-29 4.495351806232356E-5 0.0 0.0 0.0012586985057450595 0.0 30-31 8.990703612464712E-5 0.0 0.0 0.0028770251559887077 0.0 32-33 8.990703612464712E-5 0.0 0.0 0.004360491252045385 0.0 34-35 8.990703612464712E-5 4.495351806232356E-5 0.0 0.006383399564849945 0.0 36-37 1.7981407224929423E-4 8.990703612464712E-5 0.0 0.00921547120277633 0.0 38-39 2.6972110837394133E-4 8.990703612464712E-5 0.0 0.014160358189631921 0.0 40-41 2.6972110837394133E-4 8.990703612464712E-5 0.0 0.0194648733209861 8.990703612464712E-5 42-43 2.6972110837394133E-4 8.990703612464712E-5 0.0 0.024724434934277957 8.990703612464712E-5 44-45 3.5962814449858846E-4 8.990703612464712E-5 0.0 0.027106971391581105 8.990703612464712E-5 46-47 4.495351806232356E-4 8.990703612464712E-5 0.0 0.030568392282380015 8.990703612464712E-5 48-49 5.394422167478827E-4 8.990703612464712E-5 0.0 0.03600776796792117 8.990703612464712E-5 50-51 5.394422167478827E-4 8.990703612464712E-5 0.0 0.04230126049664647 1.7981407224929423E-4 52-53 5.394422167478827E-4 8.990703612464712E-5 0.0 0.05273047668710554 1.7981407224929423E-4 54-55 5.394422167478827E-4 1.7981407224929423E-4 0.0 0.05996799309513963 1.7981407224929423E-4 56-57 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.07749986513944582 1.7981407224929423E-4 58-59 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.11130491072231313 1.7981407224929423E-4 60-61 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.13710823009008685 1.7981407224929423E-4 62-63 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.15315663603833635 1.7981407224929423E-4 64-65 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.18291586499559453 1.7981407224929423E-4 66-67 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.24131048495855287 1.7981407224929423E-4 68-69 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.3224515850610469 1.7981407224929423E-4 70-71 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.39644507579163146 1.7981407224929423E-4 72-73 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.47601280276194413 2.247675903116178E-4 74-75 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.5653354431517811 2.6972110837394133E-4 76-77 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.6334400230162012 2.6972110837394133E-4 78-79 6.293492528725298E-4 1.7981407224929423E-4 0.0 0.7159746821786273 2.6972110837394133E-4 80-81 8.990703612464712E-4 1.7981407224929423E-4 0.0 0.8270548253106288 2.6972110837394133E-4 82-83 8.990703612464712E-4 1.7981407224929423E-4 0.0 0.9266718213367378 2.6972110837394133E-4 84-85 9.440238793087947E-4 1.7981407224929423E-4 0.0 1.0647240753061333 2.6972110837394133E-4 86-87 9.889773973711183E-4 1.7981407224929423E-4 0.0 1.2690827684174564 2.6972110837394133E-4 88-89 9.889773973711183E-4 1.7981407224929423E-4 0.0 1.5263067987700718 2.6972110837394133E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1560 0.0 47.830616 2 GTACCTG 1650 0.0 46.089928 1 GTACACG 280 0.0 39.042694 1 TAACGCA 145 6.6029315E-10 32.775047 4 GGTATCA 4480 0.0 32.146564 1 ATAACGC 150 9.495125E-10 31.682549 3 ACCTGGG 2335 0.0 31.343334 3 GTATCAA 6725 0.0 30.532436 1 TATAACG 155 4.562935E-8 27.595718 2 GTACATG 4960 0.0 27.40655 1 TACATGG 5155 0.0 25.353325 2 ATCAACG 8130 0.0 25.25251 3 TCAACGC 8200 0.0 25.094898 4 TATCAAC 8225 0.0 24.904182 2 CAACGCA 8280 0.0 24.56435 5 ACATGGG 5130 0.0 24.456703 3 AACGCAG 8470 0.0 24.06834 6 TACACGG 410 0.0 23.183393 2 GAGTACT 5060 0.0 22.535368 12-13 ACGCAGA 9385 0.0 21.61759 7 >>END_MODULE