FastQCFastQC Report
Thu 26 May 2016
SRR936722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112260
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55990.5033894952618992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.43892615036052723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.2907593548271088No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.19608724578785536No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.13611925269271574No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12990.11678923992591661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15650.050.388542
GTACCTG17050.046.7344171
GTACACG1800.034.667621
GGTATCA46100.034.0486561
ACCTGGG23900.032.7888683
GTATCAA69600.032.5526161
TATCACG902.411207E-531.6699072
TATAACG1405.80063E-727.1456342
ATAACGC1257.397657E-626.596743
GTACATG46200.025.870841
ATCAACG87100.025.6283153
TATCAAC88050.025.5193612
TCAACGC88350.025.2119644
ATCACGC1151.2827838E-424.7795723
CAACGCA89950.024.7623865
TAACGCA1551.4120742E-624.5131244
AACGCAG91950.024.378736
TACATGG47650.023.8271982
CCTGGGG16700.023.0360984
ACATGGG47000.022.0292153
Produced by FastQC (version 0.11.5)