FastQCFastQC Report
Thu 26 May 2016
SRR936721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120829
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.42441799774987976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39370.35125786359917527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.20208256567237287No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.13445405142086794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17350.054.7853852
GTACCTG18800.051.0632361
ACCTGGG26500.035.1515463
GTATCAA63750.033.5464941
GGTATCA40900.033.1158831
TATCACG700.00836883527.1578982
ATCAACG77450.027.061473
TATCAAC78100.027.0188062
TCAACGC78800.026.5990434
CAACGCA79750.026.4574345
AACGCAG81150.026.000996
GTACATG53100.025.5968381
TACATGG53400.025.098172
CCTGGGG18500.024.1495884
ACATGGG54450.023.566773
ACGCAGA90350.023.2523657
CGCAGAG91350.022.8937598
TGGGGAG13450.022.6127346
GCAGAGT95000.021.2636769
GAGTACT48100.021.1941712-13
Produced by FastQC (version 0.11.5)