FastQCFastQC Report
Thu 26 May 2016
SRR936721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120829
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56240.5017714566628808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.4571616187661097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.28175573615600596No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22860.20395617886403722No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.13026072665857147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12920.11527182112525639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16800.050.8938372
GTACCTG17650.050.0799641
TATAACG1600.041.56332
TAACGCA1700.039.1166574
ATAACGC1850.035.9482423
GGTATCA44250.035.6550141
ACCTGGG24550.033.4746443
GTATCAA67650.032.3838841
ACAACGC1351.1963493E-831.6686923
ATCAACG84200.025.7825263
TATCAAC85400.025.6415942
TCAACGC85050.025.5225754
GTACACG1901.1850716E-825.011641
CAACGCA86900.024.8699115
AACGCAG89650.024.2659786
ACGCAGA101500.021.4329537
GTGTAGC6450.021.3665331
CCTGGGG16500.021.3024884
CGCAGAG101900.021.2555968
GAGTACT61900.021.02672212-13
Produced by FastQC (version 0.11.5)