##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936721_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1120829 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.754007078689078 31.0 31.0 34.0 30.0 34.0 2 31.88441769440298 31.0 31.0 34.0 30.0 34.0 3 31.98907951168287 31.0 31.0 34.0 30.0 34.0 4 35.76438778796766 37.0 35.0 37.0 35.0 37.0 5 35.5349522540905 37.0 35.0 37.0 33.0 37.0 6 35.628331351169535 37.0 35.0 37.0 33.0 37.0 7 35.57572475373139 37.0 35.0 37.0 33.0 37.0 8 35.55336541078077 37.0 35.0 37.0 33.0 37.0 9 37.171637243504584 39.0 37.0 39.0 33.0 39.0 10-11 37.02566537803715 39.0 37.0 39.0 33.0 39.0 12-13 36.92186854551408 39.0 37.0 39.0 33.0 39.0 14-15 37.98691994943029 40.0 37.0 41.0 33.0 41.0 16-17 37.910688427940386 40.0 37.0 41.0 33.0 41.0 18-19 38.06294760396099 40.0 37.0 41.0 33.0 41.0 20-21 38.09827145800118 40.0 37.0 41.0 33.0 41.0 22-23 37.98440261627778 40.0 37.0 41.0 33.0 41.0 24-25 37.85206084068132 40.0 37.0 41.0 33.0 41.0 26-27 37.60953633426687 39.5 37.0 41.0 32.0 41.0 28-29 37.49776816981002 39.0 36.5 41.0 32.0 41.0 30-31 37.40383814123296 39.0 36.0 41.0 32.0 41.0 32-33 37.2002325956948 39.0 36.0 41.0 31.5 41.0 34-35 36.978862520509374 39.0 35.5 41.0 31.0 41.0 36-37 36.67144408290649 39.0 35.0 41.0 30.5 41.0 38-39 36.49397677968717 39.0 35.0 41.0 30.0 41.0 40-41 36.25952174684987 39.0 35.0 40.0 30.0 41.0 42-43 35.981869223583615 38.5 35.0 40.0 29.5 41.0 44-45 35.69123702188291 38.0 35.0 40.0 28.5 41.0 46-47 35.27822486748648 38.0 34.5 40.0 26.5 41.0 48-49 34.86680305381107 38.0 34.0 40.0 25.5 41.0 50-51 34.523945668786226 38.0 34.0 40.0 24.0 41.0 52-53 34.233760011562865 38.0 33.0 40.0 23.5 41.0 54-55 33.95280368370197 38.0 33.0 40.0 23.0 41.0 56-57 33.6206370463291 37.0 33.0 40.0 22.0 41.0 58-59 33.273240610298274 37.0 32.5 40.0 21.0 41.0 60-61 32.91500487585528 36.0 32.0 39.0 20.0 41.0 62-63 32.51013535517015 36.0 31.0 39.0 18.5 40.0 64-65 32.07739583825901 35.0 31.0 39.0 17.5 40.0 66-67 31.611210541483132 35.0 30.5 38.0 14.0 40.0 68-69 31.323432031112688 35.0 30.0 38.0 12.5 40.0 70-71 30.889489832971844 34.5 30.0 37.0 8.0 39.5 72-73 30.43072627492686 34.0 29.0 37.0 7.5 39.0 74-75 29.908537341557007 34.0 29.0 36.0 6.5 39.0 76-77 28.50557043045817 32.5 27.0 35.0 2.0 37.0 78-79 29.26817650150023 34.0 29.0 35.0 2.0 37.0 80-81 29.067474164212385 34.0 29.0 35.0 2.0 37.0 82-83 28.725460797320554 34.0 28.5 35.0 2.0 36.5 84-85 28.297438770766995 33.0 27.5 35.0 2.0 36.0 86-87 27.896351718237128 33.0 27.0 35.0 2.0 36.0 88-89 27.478645716697194 33.0 26.0 35.0 2.0 35.0 90-91 26.983289600822246 33.0 25.5 35.0 2.0 35.0 92-93 26.46573607570825 32.0 25.0 34.0 2.0 35.0 94-95 25.838789860005406 32.0 23.5 34.0 2.0 35.0 96-97 25.054217458684597 32.0 19.5 34.0 2.0 35.0 98-99 24.056225793586712 31.0 8.0 34.0 2.0 35.0 100-101 20.898386372943598 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.398839770774412 1101 2 0.4487613997907971 1101 3 0.38770172040705475 1101 4 0.2587810325276436 1101 5 0.32500492348128773 1101 6 0.35763446534550525 1101 7 0.36813776976526924 1101 8 0.24925433369742933 1101 9 0.2895628116910842 1101 10-11 0.38399051684983476 1101 12-13 0.3890762840939246 1101 14-15 0.41912576741329843 1101 16-17 0.47646214379511065 1101 18-19 0.41039642818419253 1101 20-21 0.3667998374349182 1101 22-23 0.2303129791853067 1101 24-25 0.183443438339026 1101 26-27 0.04464912373593677 1101 28-29 0.17589909641551316 1101 30-31 0.30989686805479266 1101 32-33 0.4377121531283663 1101 34-35 0.1724254757668433 1101 36-37 0.22024980103247316 1101 38-39 0.26403957012245627 1101 40-41 0.3117438729862698 1101 42-43 0.2813680244437151 1101 44-45 0.17200780028382212 1101 46-47 0.2960950474354931 1101 48-49 0.3735945751737475 1101 50-51 0.4910880290137811 1101 52-53 0.2355512609690038 1101 54-55 0.2720275637168683 1101 56-57 0.20583599236211825 1101 58-59 0.14670183850608964 1101 60-61 0.2654506730404904 1101 62-63 0.18263956791810187 1101 64-65 0.2189592204599009 1101 66-67 0.1324964703409961 1101 68-69 0.027264211281458728 1101 70-71 -0.10233537489186872 1101 72-73 0.06013574848155301 1101 74-75 0.11214523474614069 1101 76-77 0.13824871177377318 1101 78-79 0.09908770415691137 1101 80-81 0.05237565256981824 1101 82-83 0.5330984298530375 1101 84-85 0.163660134013643 1101 86-87 0.32226606851623885 1101 88-89 0.424396867396446 1101 90-91 0.3954625960964897 1101 92-93 0.3446024236521623 1101 94-95 -0.03488433836478322 1101 96-97 0.1765418215033847 1101 98-99 0.4238712751899669 1101 100-101 1.0132187451822823 1102 1 0.31583629388930845 1102 2 0.299347803100666 1102 3 0.27321401425594516 1102 4 0.2377846972731419 1102 5 0.07835595678688634 1102 6 0.162507681588977 1102 7 0.14572793175084087 1102 8 0.2231872352453692 1102 9 0.1661862264354852 1102 10-11 0.29293656128763246 1102 12-13 0.29320253509009575 1102 14-15 0.5085645638151846 1102 16-17 0.46599736541902104 1102 18-19 0.48765365163679775 1102 20-21 0.31155640410994323 1102 22-23 0.29425486280872093 1102 24-25 0.3541182828038245 1102 26-27 0.21266456938336376 1102 28-29 0.20971690411397503 1102 30-31 0.38542665640363794 1102 32-33 0.29949386227643515 1102 34-35 0.25924009717676455 1102 36-37 0.17325938338063906 1102 38-39 -0.07378480094880757 1102 40-41 0.07885678149091291 1102 42-43 0.09421246575715259 1102 44-45 0.17001471883018837 1102 46-47 0.07103938645307295 1102 48-49 0.13441966393044424 1102 50-51 -0.06850481291783694 1102 52-53 -0.007854912299926298 1102 54-55 -0.04896422179351845 1102 56-57 0.15624304769433905 1102 58-59 -0.13920371467675352 1102 60-61 0.03757417070764291 1102 62-63 0.16110827081541146 1102 64-65 0.24244764136113162 1102 66-67 0.016573760219866784 1102 68-69 -0.1375972343336862 1102 70-71 -0.12662183558721551 1102 72-73 -9.262205009328284E-4 1102 74-75 0.028265565204325327 1102 76-77 -0.36553748254139506 1102 78-79 -0.3450531794033971 1102 80-81 -0.023733861238657283 1102 82-83 -0.1278495019947279 1102 84-85 -0.11689910292088612 1102 86-87 0.08484464333091069 1102 88-89 0.05127675561950795 1102 90-91 0.19460570316077153 1102 92-93 0.060091513806074914 1102 94-95 0.08535292345244017 1102 96-97 0.4027697754247477 1102 98-99 0.4250131141965383 1102 100-101 0.7106104942947162 1103 1 0.055493667295561266 1103 2 0.03904529336158191 1103 3 0.14264654701924329 1103 4 0.13670598370605092 1103 5 -0.11497576232063267 1103 6 0.17873781479126194 1103 7 0.08324521423510589 1103 8 0.06349493152066543 1103 9 0.17052242860150812 1103 10-11 -0.05045619274877566 1103 12-13 0.06623820613761922 1103 14-15 0.05226507143667192 1103 16-17 0.210508520053736 1103 18-19 0.10270986537940274 1103 20-21 0.06996124334881415 1103 22-23 -0.008363406693447928 1103 24-25 -0.04250273183740205 1103 26-27 0.12656735191706048 1103 28-29 0.20195071087395178 1103 30-31 0.024429766892616556 1103 32-33 0.03055118496352094 1103 34-35 0.17066745486580004 1103 36-37 -0.023084323502700954 1103 38-39 0.02046401811128362 1103 40-41 0.19498540472344672 1103 42-43 0.18999826181165957 1103 44-45 0.1931521147591866 1103 46-47 -0.1677536836468292 1103 48-49 0.0355036786533347 1103 50-51 0.040676788919519424 1103 52-53 -0.07286069342523405 1103 54-55 -0.26478143794169284 1103 56-57 -0.2880647288146676 1103 58-59 -0.0878351568347 1103 60-61 -0.09733200599850278 1103 62-63 -0.14898713566146427 1103 64-65 -0.147696650368637 1103 66-67 -0.14090960919475393 1103 68-69 -0.04695606670397723 1103 70-71 -0.03154166635140143 1103 72-73 -0.21296457221399478 1103 74-75 -0.3323075900292771 1103 76-77 -0.4379236903667554 1103 78-79 -0.15284759771616407 1103 80-81 -0.22129181934806397 1103 82-83 0.09746824498540363 1103 84-85 -0.14341491111074234 1103 86-87 -0.4780689721862892 1103 88-89 -0.34206327340790565 1103 90-91 -0.33107660573613984 1103 92-93 -0.0724803634191531 1103 94-95 0.15882479365722446 1103 96-97 0.17870209829093042 1103 98-99 0.3926631691423026 1103 100-101 0.6081459583106117 1104 1 0.10960251469878202 1104 2 0.11808766184712027 1104 3 0.14585303343477918 1104 4 0.06678670785676388 1104 5 0.07526040625971575 1104 6 0.11611045067947146 1104 7 0.0756838263912698 1104 8 0.02987883444225048 1104 9 0.2067034355625097 1104 10-11 0.0943034394512523 1104 12-13 0.02211219184980706 1104 14-15 0.08105608014996335 1104 16-17 0.2607131054759151 1104 18-19 0.13149040222327812 1104 20-21 -0.003190000464933007 1104 22-23 -0.08864180483252682 1104 24-25 0.09177106845287852 1104 26-27 0.24292378363453793 1104 28-29 0.1374869299286985 1104 30-31 0.1722150712295729 1104 32-33 0.13610588337716933 1104 34-35 0.3387388646377332 1104 36-37 0.319748275287175 1104 38-39 0.3033395794907463 1104 40-41 0.49178610899157604 1104 42-43 0.37683491251686974 1104 44-45 0.3991561261832999 1104 46-47 0.27635690208671093 1104 48-49 0.2759468760920498 1104 50-51 0.13461678790025644 1104 52-53 0.3768219740251979 1104 54-55 0.515540732611548 1104 56-57 0.42634784468913267 1104 58-59 0.22971032074887177 1104 60-61 0.3325455638855175 1104 62-63 0.32731397666051265 1104 64-65 0.49417728368232616 1104 66-67 0.39789355386831105 1104 68-69 0.5363528904444372 1104 70-71 0.42079073312388715 1104 72-73 0.3540124573913772 1104 74-75 0.33874969877580696 1104 76-77 0.21294837201207528 1104 78-79 0.3978934964824852 1104 80-81 0.6478900972693253 1104 82-83 0.40737658820301803 1104 84-85 0.43472897097879937 1104 86-87 0.6318207311037654 1104 88-89 0.4396450235938616 1104 90-91 0.49238443088119155 1104 92-93 0.48688326805760695 1104 94-95 0.5958734997181345 1104 96-97 0.7345562572636801 1104 98-99 0.7859509800957376 1104 100-101 1.0441377036850135 1105 1 0.08838901290488721 1105 2 0.12963542714131648 1105 3 0.10431280710319513 1105 4 0.033916966486572164 1105 5 -0.11202383161354135 1105 6 0.011914123076294914 1105 7 0.11135830591810247 1105 8 0.07108821421341815 1105 9 -0.015304097976382991 1105 10-11 0.03289852122729542 1105 12-13 -0.21825122411381415 1105 14-15 0.1543453729247659 1105 16-17 0.09058204142456106 1105 18-19 0.13516462954741115 1105 20-21 0.1807620430549548 1105 22-23 0.17693035632771625 1105 24-25 0.18139150338051735 1105 26-27 0.30408802454507367 1105 28-29 0.0806979313903966 1105 30-31 0.17459303088256206 1105 32-33 0.19564199310975283 1105 34-35 0.19364603777082579 1105 36-37 0.05994897982763092 1105 38-39 0.11486551433566916 1105 40-41 0.08323264066199698 1105 42-43 0.184033062487984 1105 44-45 0.004718551776726088 1105 46-47 -0.10304884867735353 1105 48-49 -0.10751038910858313 1105 50-51 -0.0681541876360825 1105 52-53 -0.051372796972039225 1105 54-55 0.00461466054665749 1105 56-57 -0.25164963870752644 1105 58-59 -0.18022687264178927 1105 60-61 -0.27074093567652113 1105 62-63 -0.33178321900810914 1105 64-65 -0.44038187683520746 1105 66-67 0.001887848121072011 1105 68-69 0.13437737037805064 1105 70-71 -0.20950993046753297 1105 72-73 -0.053199997625693385 1105 74-75 -0.262873076484464 1105 76-77 -0.19372205057661418 1105 78-79 0.05778187644620303 1105 80-81 0.01680231716838776 1105 82-83 0.08340153457604416 1105 84-85 -0.1199302028268292 1105 86-87 0.031744392327730964 1105 88-89 0.1320882305275397 1105 90-91 0.08724688549148496 1105 92-93 0.04619937838680244 1105 94-95 -0.0012703046376500993 1105 96-97 -0.001803101761787218 1105 98-99 0.029566929611803516 1105 100-101 0.3961733591815424 1106 1 -0.021572478702569953 1106 2 -0.007432511692204713 1106 3 0.030067645179901348 1106 4 0.09485792846055574 1106 5 0.07328997226890976 1106 6 -0.0552711668812762 1106 7 0.0788918883432288 1106 8 0.032797845126964376 1106 9 0.021990176455190635 1106 10-11 0.013124815945950274 1106 12-13 0.08579133951496942 1106 14-15 0.030182378590644987 1106 16-17 -0.04650293049129317 1106 18-19 -0.01867987339999644 1106 20-21 -0.16335527536557493 1106 22-23 -0.14541459493041486 1106 24-25 -0.12702004586689952 1106 26-27 -0.1687547696435132 1106 28-29 -0.1265047459376234 1106 30-31 -0.06274900431186836 1106 32-33 -0.16737114552863375 1106 34-35 -0.11132142222869135 1106 36-37 -0.0810379305892539 1106 38-39 0.05881713493125318 1106 40-41 0.06742594025620008 1106 42-43 0.24032705893965556 1106 44-45 0.09488692038897995 1106 46-47 0.2496783522012791 1106 48-49 0.22218944710129307 1106 50-51 0.10397260390352159 1106 52-53 -0.10324943385125351 1106 54-55 0.2233930206287411 1106 56-57 0.24659595381209698 1106 58-59 0.1101292686701072 1106 60-61 -0.0059514509476983335 1106 62-63 0.14936933472785796 1106 64-65 0.3058016907370771 1106 66-67 0.2001638276595692 1106 68-69 0.10915784801293071 1106 70-71 0.40335944895596043 1106 72-73 0.2960027178564246 1106 74-75 0.3194266894128539 1106 76-77 0.020412069276332545 1106 78-79 0.1860368984802001 1106 80-81 0.33479838184505084 1106 82-83 0.4730336336240981 1106 84-85 0.32407967091033996 1106 86-87 0.19354572935656478 1106 88-89 0.1317915142332815 1106 90-91 0.19136705184338254 1106 92-93 0.15027662137121212 1106 94-95 0.270699305618173 1106 96-97 0.10849428222929802 1106 98-99 0.3760542311082631 1106 100-101 0.6594148773878636 1107 1 -0.05872852142918816 1107 2 0.0793932255017964 1107 3 -0.007376748949187828 1107 4 -0.15752346787944305 1107 5 -0.016556462295966412 1107 6 0.04588090685144408 1107 7 -0.11697130123616262 1107 8 -0.26975869305795186 1107 9 -0.1270575313842386 1107 10-11 -0.04412825403086629 1107 12-13 -0.10008610114990546 1107 14-15 0.07540893089346667 1107 16-17 0.007337870233250499 1107 18-19 -0.03131972050049825 1107 20-21 -0.10606857549077375 1107 22-23 -0.14276915394589196 1107 24-25 -0.14956106768303812 1107 26-27 -0.24538498727223157 1107 28-29 -0.20250498567550324 1107 30-31 -0.15137976801023 1107 32-33 -0.2329471158011316 1107 34-35 -0.2532599028232383 1107 36-37 -0.27627347306281536 1107 38-39 -0.12224057530554688 1107 40-41 -0.030095103402736356 1107 42-43 -0.44969018886940404 1107 44-45 -0.3686113215331872 1107 46-47 -0.0903665320585958 1107 48-49 -0.2732055876606978 1107 50-51 -0.027811645317335376 1107 52-53 -0.15820102011693393 1107 54-55 -0.13638262466573536 1107 56-57 -0.35730411544552254 1107 58-59 -0.11844948522562504 1107 60-61 0.057499509335720234 1107 62-63 -0.13696127713649986 1107 64-65 -0.05214994880369517 1107 66-67 -0.04298466332361883 1107 68-69 0.008686696471951194 1107 70-71 -0.19634454134797608 1107 72-73 -0.2731723174383198 1107 74-75 0.027755583155695263 1107 76-77 0.027358807509198613 1107 78-79 -0.04574335510921301 1107 80-81 0.1316325098650779 1107 82-83 0.08449410351035525 1107 84-85 0.08209657243134316 1107 86-87 0.061696218155475435 1107 88-89 0.13334714674173753 1107 90-91 -0.003081018266630764 1107 92-93 -0.043358004770869485 1107 94-95 0.19716070781843342 1107 96-97 0.07483907858528127 1107 98-99 0.2681222367554241 1107 100-101 0.3066625532622389 1108 1 -0.005869048535284804 1108 2 0.074768102527635 1108 3 0.012796191557207237 1108 4 -0.10025893103897232 1108 5 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-0.9886948914994207 2316 98-99 -0.8095719423759533 2316 100-101 -1.0407018367179397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 20.0 5 51.0 6 133.0 7 278.0 8 656.0 9 1218.0 10 2160.0 11 3389.0 12 4799.0 13 6515.0 14 7439.0 15 9237.0 16 11381.0 17 14367.0 18 12657.0 19 8788.0 20 7987.0 21 8156.0 22 8706.0 23 9702.0 24 11448.0 25 13465.0 26 16478.0 27 20200.0 28 24909.0 29 30328.0 30 36800.0 31 44259.0 32 54652.0 33 67984.0 34 87739.0 35 114491.0 36 157214.0 37 199281.0 38 117419.0 39 6514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35589535240734 18.886512770347945 14.61521160148803 22.142380275756686 2 16.151424737322976 21.005953060758337 39.47480656121517 23.36781564070352 3 19.367347357582666 25.98081859573705 29.914532202328214 24.737301844352068 4 13.192181102636189 17.323163425447195 36.343561829120205 33.14109364279641 5 13.387977898304722 36.76472293920784 33.605782914101205 16.241516248386226 6 30.51874242745975 35.74105168866532 18.650798607455574 15.089407276419356 7 28.340719235494443 32.671888396891944 21.013285701922417 17.9741066656912 8 27.146335435646296 33.284024592511436 20.209148763995223 19.36049120784705 9 26.84317591741687 16.8238149195671 20.790856612628367 35.54215255038767 10-11 25.031126639362363 26.614425362757505 27.192348133003875 21.162099864876257 12-13 26.2622288245607 24.109464987531396 27.913348768530042 21.714957419377864 14-15 22.80472517520889 25.30627540027034 27.09677419354839 24.79222523097238 16-17 21.78199005618767 28.346601415509937 27.629835005877514 22.241573522424883 18-19 21.50698277792598 26.2827781936406 30.82437196039717 21.385867068036248 20-21 22.315982188183924 25.491221230000292 31.00669236788127 21.186104213934506 22-23 21.974028592726953 25.49130784491031 30.97908252918984 21.5555810331729 24-25 21.914882891595934 25.728874516481454 31.04444467245201 21.311797919470603 26-27 21.655554539391357 25.881434457037443 30.88900735876371 21.574003644807487 28-29 21.431417065241686 26.159934723070478 31.032461353354208 21.376186858333625 30-31 21.684701897859192 25.665776392278655 30.99680545103097 21.65271625883118 32-33 21.496907632241992 25.916870088434933 30.83222700441818 21.75399527490489 34-35 22.257120282460143 25.89383028567681 30.460652908007273 21.388396523855775 36-37 22.316265585676103 25.676842296403006 30.369576885171373 21.63731523274952 38-39 21.865116596555705 26.02443867733656 30.427130331433222 21.68331439467451 40-41 22.11970712265811 25.83117306627659 30.397288390856435 21.651831420208858 42-43 22.018240168162144 26.026500169964393 29.904766408255362 22.0504932536181 44-45 22.020112979933863 26.25835494328475 29.756557373026943 21.964974703754443 46-47 22.460929571294805 26.452887306131135 29.085002745736748 22.00118037683731 48-49 22.505917526674597 26.540140593117734 28.81721734832351 22.136724531884163 50-51 22.440956122264165 26.837294870982735 28.62759285843655 22.094156148316543 52-53 22.79763436936368 26.94694550903573 28.20390426306912 22.05151585853147 54-55 22.31344715135239 26.871104885371544 28.420183887329976 22.39526407594609 56-57 22.4661027307856 27.198292667911595 28.190295794213664 22.14530880708914 58-59 22.69876880154994 26.81549242807823 28.25778660862575 22.22795216174608 60-61 22.555748971924093 26.88232244778873 28.682091002450967 21.87983757783621 62-63 22.481124718722175 26.84960804012927 28.635688780472137 22.033578460676424 64-65 22.336413615940675 26.89290601085631 28.4338652334348 22.336815139768213 66-67 22.247065798648364 27.19482365348386 28.127602794634683 22.430507753233098 68-69 22.344080332371664 26.999353582974916 28.385737426207413 22.270828658446003 70-71 22.422798145656603 27.04067885527485 28.073486743728743 22.4630362553398 72-73 22.41907249883567 27.086467205201913 28.116116978849377 22.378343317113035 74-75 22.34149100903892 27.465359582677717 27.75908765846884 22.434061749814525 76-77 22.380724865196918 27.25130185853606 27.865505176429544 22.502468099837483 78-79 22.41449688501663 27.384819223897377 27.78569628187198 22.414987609214016 80-81 22.421350216881077 27.562522556925984 27.62609461038361 22.39003261580932 82-83 22.534554029734622 27.52906232350477 27.59142954778855 22.344954098972057 84-85 22.35627712425765 27.502123543855646 27.50163280893102 22.63996652295569 86-87 22.551027555080495 27.290312248085392 27.528224473508228 22.63043572332588 88-89 22.428868748201502 27.41210508352833 27.368071185903798 22.790954982366365 90-91 22.37821298740236 27.398263206643076 27.620091622452676 22.60343218350189 92-93 22.501582554472662 27.264368903150345 27.603274226020453 22.630774316356543 94-95 22.25908063441873 27.14280042344546 27.60626174657423 22.991857195561586 96-97 22.296637185196154 27.203994942974635 27.552936830901032 22.946431040928182 98-99 22.069348560721814 27.207655331630658 27.35487324760492 23.368122860042604 100-101 21.88419840129053 27.31733910075046 27.444215139648403 23.354247358310605 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 410.0 1 344.0 2 815.5 3 1144.5 4 707.0 5 490.0 6 637.0 7 1082.0 8 1547.0 9 1808.5 10 1718.0 11 1502.5 12 1519.0 13 1629.0 14 1830.0 15 2009.0 16 2132.0 17 2249.0 18 2354.0 19 2570.0 20 2790.0 21 3067.5 22 3517.0 23 4032.5 24 4678.0 25 5631.5 26 6877.5 27 8335.5 28 9828.0 29 11507.5 30 13646.0 31 16095.5 32 18740.5 33 20589.0 34 22164.5 35 23887.5 36 26013.5 37 28069.5 38 28815.5 39 29332.5 40 30167.0 41 32311.0 42 34621.5 43 35964.5 44 37374.5 45 38929.0 46 39270.5 47 39736.5 48 42788.0 49 43444.0 50 41187.5 51 39144.0 52 37449.0 53 37328.5 54 37102.5 55 34705.0 56 31347.5 57 28380.5 58 26301.0 59 23435.5 60 19503.5 61 16254.0 62 13582.0 63 10600.0 64 7747.0 65 5884.0 66 4465.0 67 3679.0 68 2921.0 69 2092.0 70 1601.5 71 1228.0 72 1048.0 73 911.0 74 712.5 75 574.5 76 404.0 77 241.5 78 159.0 79 118.0 80 76.0 81 45.5 82 29.5 83 22.0 84 15.0 85 7.0 86 5.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03756148350908122 2 0.005442400223406068 3 0.004996301844438358 4 5.353180547612526E-4 5 8.921967579354211E-4 6 2.676590273806263E-4 7 0.0 8 0.0 9 0.0016951738400772997 10-11 4.014885410709395E-4 12-13 0.0012490754611095894 14-15 0.0021412722190450105 16-17 0.0028104197874965764 18-19 0.0 20-21 0.0 22-23 0.001739783677974071 24-25 7.583672442451079E-4 26-27 0.005040911682335129 28-29 0.005843888764477008 30-31 0.0012044656232128182 32-33 4.4609837896771054E-4 34-35 0.00932345612042515 36-37 0.01146472833947016 38-39 0.00941267579621869 40-41 0.0016505640021805287 42-43 5.353180547612526E-4 44-45 9.368065958321922E-4 46-47 4.460983789677105E-5 48-49 5.353180547612526E-4 50-51 8.921967579354211E-4 52-53 0.00254276076011595 54-55 0.0030334689769804316 56-57 0.0014275148126966736 58-59 9.368065958321922E-4 60-61 0.004104105086502937 62-63 0.004550203465470647 64-65 0.008788138065663897 66-67 0.003434957518051371 68-69 0.0031672984906707443 70-71 8.92196757935421E-5 72-73 9.81416433728963E-4 74-75 0.0057546690886834655 76-77 0.0024535410843224076 78-79 0.0032565181664642867 80-81 0.0047732526549545025 82-83 0.004282544438090021 84-85 0.005442400223406068 86-87 0.0033457378422578287 88-89 0.009055797093044524 90-91 0.01275841363847652 92-93 0.0012936852990063606 94-95 0.0020966623811482393 96-97 0.0010706361095225052 98-99 0.003702616545431997 100-101 0.004460983789677105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1120829.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.61819204826976 #Duplication Level Percentage of deduplicated Percentage of total 1 84.85162836404434 53.981071888721864 2 9.25386307118426 11.774280761019833 3 2.3084185449393937 4.405722429592253 4 1.0137912589947506 2.5798226804634092 5 0.5461069965658607 1.737116989321536 6 0.368052765577322 1.404891091463693 7 0.26516829489253624 1.1808669256709918 8 0.19167328812991505 0.9755126443817822 9 0.14374231423585906 0.823016353726766 >10 0.9450993177738745 11.444347852296898 >50 0.07606738391609144 3.295114465824659 >100 0.034280965286714524 4.1693474042230605 >500 0.001123965044838313 0.44359681162430153 >1k 7.024781530239457E-4 0.8246270771802953 >5k 2.8099126120957824E-4 0.9606646244888158 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5624 0.5017714566628808 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 0.4571616187661097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3158 0.28175573615600596 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2286 0.20395617886403722 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.13026072665857147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1292 0.11527182112525639 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 8.92196757935421E-5 0.0 0.0 0.0 8.92196757935421E-5 16-17 1.784393515870842E-4 0.0 0.0 0.0 1.784393515870842E-4 18-19 2.2304918948385527E-4 0.0 0.0 0.0 1.784393515870842E-4 20-21 2.676590273806263E-4 0.0 0.0 4.460983789677105E-5 2.676590273806263E-4 22-23 5.353180547612526E-4 0.0 0.0 2.676590273806263E-4 2.676590273806263E-4 24-25 5.353180547612526E-4 0.0 0.0 4.460983789677105E-4 2.676590273806263E-4 26-27 5.353180547612526E-4 0.0 0.0 5.799278926580237E-4 2.676590273806263E-4 28-29 5.353180547612526E-4 0.0 0.0 0.0014721246505934446 2.676590273806263E-4 30-31 6.245377305547947E-4 0.0 0.0 0.0031672984906707443 2.676590273806263E-4 32-33 6.245377305547947E-4 0.0 0.0 0.005977718278167321 2.676590273806263E-4 34-35 6.691475684515657E-4 0.0 0.0 0.007895941307728476 2.676590273806263E-4 36-37 8.029770821418789E-4 0.0 0.0 0.011241679149986306 2.676590273806263E-4 38-39 8.92196757935421E-4 0.0 0.0 0.016773299049185914 2.676590273806263E-4 40-41 9.368065958321921E-4 0.0 0.0 0.021858820569417813 2.676590273806263E-4 42-43 9.81416433728963E-4 0.0 0.0 0.026855122413856174 2.676590273806263E-4 44-45 9.81416433728963E-4 0.0 0.0 0.02939788317397212 2.676590273806263E-4 46-47 0.0011598557853160472 0.0 0.0 0.03301128004361058 2.676590273806263E-4 48-49 0.0011598557853160472 0.0 0.0 0.03881055897019081 2.676590273806263E-4 50-51 0.0011598557853160472 0.0 0.0 0.04492210676204845 2.676590273806263E-4 52-53 0.0011598557853160472 0.0 0.0 0.05576229737096382 2.676590273806263E-4 54-55 0.0011598557853160472 0.0 0.0 0.06468426495031802 2.676590273806263E-4 56-57 0.0012490754611095894 0.0 0.0 0.08632003633025198 2.676590273806263E-4 58-59 0.0012490754611095894 0.0 0.0 0.12017890329390121 2.676590273806263E-4 60-61 0.0012490754611095894 0.0 0.0 0.14774778311410572 2.676590273806263E-4 62-63 0.0013382951369031316 0.0 0.0 0.16416420346011745 2.676590273806263E-4 64-65 0.0013382951369031316 8.92196757935421E-5 0.0 0.19726470317952158 2.676590273806263E-4 66-67 0.0014721246505934446 8.92196757935421E-5 0.0 0.2586478401254786 2.676590273806263E-4 68-69 0.0015167344884902158 8.92196757935421E-5 0.0 0.341176040234505 2.676590273806263E-4 70-71 0.0015613443263869868 8.92196757935421E-5 0.0 0.4157190793600094 2.676590273806263E-4 72-73 0.001650564002180529 8.92196757935421E-5 0.0 0.493161757948804 2.676590273806263E-4 74-75 0.0016951738400773 8.92196757935421E-5 0.0 0.5839427780687331 2.676590273806263E-4 76-77 0.0016951738400773 8.92196757935421E-5 0.0 0.6492961905875027 2.676590273806263E-4 78-79 0.0016951738400773 8.92196757935421E-5 0.0 0.7336533940502967 2.676590273806263E-4 80-81 0.0018736131916643841 8.92196757935421E-5 0.0 0.8461147953880566 2.676590273806263E-4 82-83 0.0018736131916643841 8.92196757935421E-5 0.0 0.9513494029865395 2.676590273806263E-4 84-85 0.0018736131916643841 8.92196757935421E-5 0.0 1.0941008842562068 2.676590273806263E-4 86-87 0.001918223029561155 8.92196757935421E-5 0.0 1.3018935091793664 3.1226886527739735E-4 88-89 0.001962832867457926 8.92196757935421E-5 0.0 1.5582662475721096 3.568787031741684E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1680 0.0 50.893837 2 GTACCTG 1765 0.0 50.079964 1 TATAACG 160 0.0 41.5633 2 TAACGCA 170 0.0 39.116657 4 ATAACGC 185 0.0 35.948242 3 GGTATCA 4425 0.0 35.655014 1 ACCTGGG 2455 0.0 33.474644 3 GTATCAA 6765 0.0 32.383884 1 ACAACGC 135 1.1963493E-8 31.668692 3 ATCAACG 8420 0.0 25.782526 3 TATCAAC 8540 0.0 25.641594 2 TCAACGC 8505 0.0 25.522575 4 GTACACG 190 1.1850716E-8 25.01164 1 CAACGCA 8690 0.0 24.869911 5 AACGCAG 8965 0.0 24.265978 6 ACGCAGA 10150 0.0 21.432953 7 GTGTAGC 645 0.0 21.366533 1 CCTGGGG 1650 0.0 21.302488 4 CGCAGAG 10190 0.0 21.255596 8 GAGTACT 6190 0.0 21.026722 12-13 >>END_MODULE