FastQCFastQC Report
Thu 26 May 2016
SRR936720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130590
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47350.4188078790719889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.3354001008323088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.20785607514660487No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.13983849140714139No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11650.10304354363650838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG19200.054.44951
TACCTGG19400.052.9036982
CGATACC500.001612539438.0156484
ACCTGGG27450.036.180653
GGTATCA43550.034.9167861
GTATCAA63550.030.3586851
ATAACGC1153.852123E-628.9249523
GTACATG47800.027.8357261
TACATGG49700.026.5780052
ACATGGG48900.025.5575563
ATCAACG76600.024.9384653
TCAACGC77250.024.9131664
TATCAAC76900.024.8389762
CAACGCA77850.024.5391255
AACGCAG79600.024.1798066
CCTGGGG19100.023.8841784
TAACGCA1503.0235276E-522.1757974
TATAACG1503.0255947E-522.1738322
CATGGGG24100.021.8866024
CATGGGA24150.021.841294
Produced by FastQC (version 0.11.5)