FastQCFastQC Report
Thu 26 May 2016
SRR936720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130590
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58020.5131833821279156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50090.44304301294014625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.276669703429183No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.207236929390849No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15120.13373548324326237No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13630.12055652358503083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17200.052.7481272
GTACCTG18800.049.5375861
TATAACG951.9279469E-840.0007822
GGTATCA48300.033.6446151
ACCTGGG27000.032.5411723
GTATCAA70800.029.9321731
ATAACGC1601.9081199E-929.6828293
TAACGCA1303.0445153E-729.2222924
TATCACG1004.952377E-528.5005572
GTACATG47600.026.6526811
CCTGGGG17500.026.5922834
GTACACG2059.531504E-1025.49621
GCACCGT3250.024.8389476
TACATGG50100.024.7459932
ATCAACG86100.024.3806023
TCAACGC87200.024.1243064
TATCAAC87550.024.0895612
ACATGGG49500.024.0820673
CAACGCA89200.023.7431135
AACGCAG91100.023.4564236
Produced by FastQC (version 0.11.5)