##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936720_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1130590 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.773305972987554 31.0 31.0 34.0 30.0 34.0 2 31.92039200771279 31.0 31.0 34.0 30.0 34.0 3 32.00077923915831 31.0 31.0 34.0 30.0 34.0 4 35.73319859542363 37.0 35.0 37.0 35.0 37.0 5 35.55609814344723 37.0 35.0 37.0 33.0 37.0 6 35.63776435312536 37.0 35.0 37.0 33.0 37.0 7 35.58026517128225 37.0 35.0 37.0 33.0 37.0 8 35.548831141262525 37.0 35.0 37.0 33.0 37.0 9 37.157902511078284 39.0 37.0 39.0 33.0 39.0 10-11 37.046759214215584 39.0 37.0 39.0 33.0 39.0 12-13 36.933649687331396 39.0 37.0 39.0 33.0 39.0 14-15 38.02762141890517 40.0 37.0 41.0 33.0 41.0 16-17 37.927809373866744 40.0 37.0 41.0 33.0 41.0 18-19 38.07967167585066 40.0 37.0 41.0 33.0 41.0 20-21 38.08845868086574 40.0 37.0 41.0 33.0 41.0 22-23 37.98165294226908 40.0 37.0 41.0 33.0 41.0 24-25 37.84302709204928 40.0 37.0 41.0 32.5 41.0 26-27 37.62167187043933 39.5 37.0 41.0 32.0 41.0 28-29 37.506688543150034 39.0 36.5 41.0 32.0 41.0 30-31 37.4141939164507 39.0 36.0 41.0 32.0 41.0 32-33 37.19924066195526 39.0 36.0 41.0 31.5 41.0 34-35 36.97389327696159 39.0 35.5 41.0 31.0 41.0 36-37 36.73689401109155 39.0 35.0 41.0 30.5 41.0 38-39 36.54893595379404 39.0 35.0 41.0 30.0 41.0 40-41 36.32629910046967 39.0 35.0 40.5 30.0 41.0 42-43 36.015793081488425 39.0 35.0 40.0 29.5 41.0 44-45 35.71221884148984 38.0 35.0 40.0 28.5 41.0 46-47 35.29528697405779 38.0 34.5 40.0 26.5 41.0 48-49 34.85365074872412 38.0 34.0 40.0 25.5 41.0 50-51 34.53903050619588 38.0 34.0 40.0 24.0 41.0 52-53 34.16753951476663 38.0 33.0 40.0 23.0 41.0 54-55 33.95943976154044 38.0 33.0 40.0 23.0 41.0 56-57 33.659050584208245 37.0 33.0 40.0 22.0 41.0 58-59 33.2823914947063 37.0 32.5 40.0 21.0 41.0 60-61 32.8683501534597 36.0 32.0 39.0 19.5 41.0 62-63 32.525854642266424 36.0 31.0 39.0 18.5 40.0 64-65 32.1016177394104 35.0 31.0 39.0 17.5 40.0 66-67 31.681952785713655 35.0 31.0 38.0 14.5 40.0 68-69 31.31824755216303 35.0 30.0 38.0 13.0 40.0 70-71 30.9456633262279 35.0 30.0 37.0 9.0 39.5 72-73 30.491258546422664 34.0 29.5 37.0 7.5 39.0 74-75 29.994821288000068 34.0 29.0 36.0 7.0 39.0 76-77 28.52148568446563 32.5 27.0 35.0 3.5 37.0 78-79 29.274933884078223 34.0 29.0 35.0 2.0 37.0 80-81 29.045486869687508 34.0 29.0 35.0 2.0 37.0 82-83 28.74733590426238 34.0 29.0 35.0 2.0 36.5 84-85 28.362681874065753 33.5 28.0 35.0 2.0 36.0 86-87 27.964096179870687 33.0 27.0 35.0 2.0 36.0 88-89 27.496134761496208 33.0 26.0 35.0 2.0 35.0 90-91 27.024806074704358 33.0 25.5 35.0 2.0 35.0 92-93 26.400292767493077 32.0 25.0 34.0 2.0 35.0 94-95 25.843777142907683 32.0 23.5 34.0 2.0 35.0 96-97 25.123355062401046 32.0 19.5 34.0 2.0 35.0 98-99 24.228008385002518 31.0 9.5 34.0 2.0 35.0 100-101 21.090965778929586 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3800619127425158 1101 2 0.36971936711783115 1101 3 0.29741190146215857 1101 4 0.2596966812461261 1101 5 0.2635778795806303 1101 6 0.23821317705525757 1101 7 0.2790323395748402 1101 8 0.2348652908542519 1101 9 0.23355770953489952 1101 10-11 0.32468871281955813 1101 12-13 0.3102092457034118 1101 14-15 0.6493625582220446 1101 16-17 0.5166418307875418 1101 18-19 0.43325528635189414 1101 20-21 0.4236365236587645 1101 22-23 0.49809201349037124 1101 24-25 0.3003484095667659 1101 26-27 0.5256287861020041 1101 28-29 0.5359540182998828 1101 30-31 0.5498670493555835 1101 32-33 0.45694430417137966 1101 34-35 0.4936796904986238 1101 36-37 0.3175697950426368 1101 38-39 0.1644225883012851 1101 40-41 0.3678817902371918 1101 42-43 0.5719859801270388 1101 44-45 0.6638403237422779 1101 46-47 0.6770222900851692 1101 48-49 0.6263138532062982 1101 50-51 0.6921150234828275 1101 52-53 0.857544200429075 1101 54-55 0.5546391520045546 1101 56-57 0.5951142026126561 1101 58-59 0.3525607087679248 1101 60-61 0.48813268960265077 1101 62-63 0.6283622029151772 1101 64-65 0.7793969060584018 1101 66-67 0.5627119578130184 1101 68-69 0.7619078911672013 1101 70-71 0.6909371941922124 1101 72-73 0.9181038518776923 1101 74-75 0.7471996580195679 1101 76-77 0.5113850372770017 1101 78-79 0.5244640339126967 1101 80-81 0.389834778986792 1101 82-83 0.4641607570932358 1101 84-85 0.5438222557398156 1101 86-87 0.7482814466985488 1101 88-89 0.7265666629667109 1101 90-91 0.6758851286287175 1101 92-93 0.7384220604864673 1101 94-95 0.5851330894271918 1101 96-97 0.7849927892336659 1101 98-99 0.9088316111488872 1101 100-101 1.37860255069808 1102 1 0.3105971006433883 1102 2 0.3318340481233264 1102 3 0.3011936194553151 1102 4 0.29247157052014927 1102 5 0.21254024015478024 1102 6 0.15812904012536677 1102 7 0.22399523341122318 1102 8 0.20959903005939395 1102 9 0.3531773158096172 1102 10-11 0.32736835553385646 1102 12-13 0.3656455578988229 1102 14-15 0.4411340257300864 1102 16-17 0.42235588372862765 1102 18-19 0.4396359170826116 1102 20-21 0.26567091907271134 1102 22-23 0.4289079047048787 1102 24-25 0.29689701790046996 1102 26-27 0.4072853059734314 1102 28-29 0.42915862805561744 1102 30-31 0.5039079920447023 1102 32-33 0.4207891248619475 1102 34-35 0.3536620632192964 1102 36-37 0.36744610783055265 1102 38-39 0.4044439187905553 1102 40-41 0.5272753525770284 1102 42-43 0.7727592103630059 1102 44-45 0.579536008762048 1102 46-47 0.7442814703426137 1102 48-49 0.9396202271712539 1102 50-51 0.5248002950762398 1102 52-53 0.7123280070326388 1102 54-55 0.4617078391425693 1102 56-57 0.6257641900217337 1102 58-59 0.682981837802572 1102 60-61 0.7732250476586131 1102 62-63 0.8372624976906806 1102 64-65 0.7998467720022049 1102 66-67 0.6914207225703102 1102 68-69 0.7452130801049783 1102 70-71 1.013435675876135 1102 72-73 0.8932560538063221 1102 74-75 0.8897917568508369 1102 76-77 0.886848308840797 1102 78-79 0.8331369515977478 1102 80-81 0.8826275917969326 1102 82-83 0.858188220098171 1102 84-85 0.7623797814030624 1102 86-87 1.0671907773063154 1102 88-89 0.9646889873975262 1102 90-91 0.6908705379815459 1102 92-93 0.8340211657909471 1102 94-95 1.1294331086838838 1102 96-97 1.0285032841306467 1102 98-99 0.7499135409656539 1102 100-101 1.6144925801756322 1103 1 0.19501823506451998 1103 2 0.24076955705883307 1103 3 0.1814836997453959 1103 4 0.14668032472890502 1103 5 0.30480908242362403 1103 6 0.22717850917484128 1103 7 0.15322341263939876 1103 8 0.03731205777242508 1103 9 0.21516902780133051 1103 10-11 0.1866729398384379 1103 12-13 0.3735644908177491 1103 14-15 0.36562250021855647 1103 16-17 0.3062201675929188 1103 18-19 0.3069306593773504 1103 20-21 0.2791777825795734 1103 22-23 0.1661715669685364 1103 24-25 0.33331892932238105 1103 26-27 0.29021718890530934 1103 28-29 0.20728605618304385 1103 30-31 0.12355963669635628 1103 32-33 0.26217089124371995 1103 34-35 0.18470971926695512 1103 36-37 0.3122078275922746 1103 38-39 0.20461874396537638 1103 40-41 0.39916734946901755 1103 42-43 0.27583483843863377 1103 44-45 0.501402105628145 1103 46-47 0.563363314424457 1103 48-49 0.31125322380425047 1103 50-51 0.44975497003091647 1103 52-53 0.3535090482136809 1103 54-55 0.25209245452718676 1103 56-57 0.31040547466302115 1103 58-59 0.5453296751504126 1103 60-61 0.4253682748018406 1103 62-63 0.6553536657818526 1103 64-65 0.5602521074075355 1103 66-67 0.3739511551007446 1103 68-69 0.4069457918376891 1103 70-71 0.39453513197559076 1103 72-73 0.19969868524895773 1103 74-75 0.27851877057785046 1103 76-77 0.3506594976519821 1103 78-79 0.08649100495179951 1103 80-81 0.047047431216771685 1103 82-83 0.06698127889123029 1103 84-85 0.40967970370298445 1103 86-87 0.6951045302200711 1103 88-89 0.6691437918523313 1103 90-91 0.6676317397427489 1103 92-93 0.7379450197148003 1103 94-95 0.6190795733303496 1103 96-97 0.7096480652754238 1103 98-99 0.629277695329808 1103 100-101 0.9178336835167364 1104 1 0.25620478940460245 1104 2 0.1972807268480139 1104 3 0.22389816249181038 1104 4 0.22243929758330694 1104 5 0.15193804843225678 1104 6 0.12562912878728127 1104 7 0.15231739534333855 1104 8 0.09043387064527053 1104 9 0.1901812523986166 1104 10-11 0.18303385186325016 1104 12-13 0.049959492005278605 1104 14-15 0.14282746829489668 1104 16-17 0.3143834043868523 1104 18-19 0.2298440952575902 1104 20-21 0.2913332235731829 1104 22-23 0.1169864236962539 1104 24-25 0.18873302882361287 1104 26-27 0.2416010133181814 1104 28-29 0.32355874154286823 1104 30-31 0.33436513287529124 1104 32-33 0.24670677567586807 1104 34-35 0.18136891146556877 1104 36-37 0.12914238757662844 1104 38-39 0.1514595587522578 1104 40-41 0.12306923135845693 1104 42-43 0.2363085241197922 1104 44-45 0.2817117721411364 1104 46-47 0.15312992939853842 1104 48-49 0.37014120458019306 1104 50-51 0.18764563323278338 1104 52-53 0.18186731972548387 1104 54-55 -0.03717679397443163 1104 56-57 -0.17799862162488722 1104 58-59 -0.20676352517929786 1104 60-61 0.10527278323331757 1104 62-63 0.15520766916282724 1104 64-65 0.1127860917879957 1104 66-67 0.15244494500532468 1104 68-69 0.2442377990467115 1104 70-71 0.3254280864162631 1104 72-73 0.4820474145893847 1104 74-75 0.40554875227588383 1104 76-77 -0.020285428790870697 1104 78-79 -0.05310271928922816 1104 80-81 0.3436961451891385 1104 82-83 0.026796582689936344 1104 84-85 0.4657790792007006 1104 86-87 0.418189110462329 1104 88-89 0.2765023008705896 1104 90-91 0.3522689136952728 1104 92-93 0.4065962624324335 1104 94-95 0.3587784291080389 1104 96-97 0.38581079911450544 1104 98-99 0.3734249074230789 1104 100-101 1.0969924915137206 1105 1 0.06152543401195132 1105 2 0.0573108979200434 1105 3 0.1466520082772682 1105 4 0.24838436707597822 1105 5 0.3032456325914197 1105 6 0.2682931455079398 1105 7 0.042108831821600745 1105 8 0.18513682925753727 1105 9 0.16489228399613864 1105 10-11 0.1357962837474389 1105 12-13 0.20846092701262364 1105 14-15 0.3870472450563298 1105 16-17 0.22758273528689443 1105 18-19 0.2233395268373357 1105 20-21 0.28733367511303953 1105 22-23 0.26482330834492984 1105 24-25 0.2908549240595306 1105 26-27 0.1837207876850826 1105 28-29 0.25593620149436447 1105 30-31 0.27466848555870627 1105 32-33 0.1729484247541464 1105 34-35 0.20885297929232394 1105 36-37 0.15114161448566676 1105 38-39 0.11066498544031589 1105 40-41 0.2337070998166908 1105 42-43 0.12801318968953268 1105 44-45 0.3309607450590093 1105 46-47 0.2232352544901275 1105 48-49 0.2071845763514304 1105 50-51 0.5383518484052061 1105 52-53 0.5445663649923489 1105 54-55 0.29330111991144747 1105 56-57 -0.13111886463543243 1105 58-59 -0.017339820021142316 1105 60-61 0.009099583491931185 1105 62-63 -0.03709083972942295 1105 64-65 0.19073008032161454 1105 66-67 0.12447855051684442 1105 68-69 0.06297225598451561 1105 70-71 0.1979224678237692 1105 72-73 0.10834790067887923 1105 74-75 0.13258880591334332 1105 76-77 -0.011918258474825194 1105 78-79 0.005589936004565033 1105 80-81 0.03364083155708286 1105 82-83 0.34007356476174877 1105 84-85 0.2621356314886718 1105 86-87 0.5867403858913747 1105 88-89 0.4726293203996903 1105 90-91 0.5749444241576604 1105 92-93 0.5369178477478123 1105 94-95 0.6588413710270196 1105 96-97 0.8795429470416991 1105 98-99 0.7331249754961355 1105 100-101 1.3939822258235672 1106 1 0.10200090580865862 1106 2 0.11937209621183342 1106 3 0.21245086374008437 1106 4 0.09203602237817421 1106 5 0.07111897029314207 1106 6 -0.021609060713650763 1106 7 0.05078465194375781 1106 8 -0.047310930520289673 1106 9 0.03672557183493552 1106 10-11 0.12410922933894852 1106 12-13 0.08023748992194868 1106 14-15 0.17364152842107927 1106 16-17 0.13785455427316862 1106 18-19 0.2463516274680444 1106 20-21 -0.060928169911697694 1106 22-23 -0.10486062034805599 1106 24-25 -0.08433235415458995 1106 26-27 -0.13785558779131435 1106 28-29 -0.20780522358713682 1106 30-31 0.05574463654190254 1106 32-33 -0.07139025539082411 1106 34-35 0.08659941129426585 1106 36-37 -0.1643428473528914 1106 38-39 0.07482308091283585 1106 40-41 0.3669993390900075 1106 42-43 0.2466477071872859 1106 44-45 0.013839167377142303 1106 46-47 -0.06780695092012223 1106 48-49 -0.3410766498558999 1106 50-51 -0.14538604254128984 1106 52-53 -0.37659010849097996 1106 54-55 0.02441429680315821 1106 56-57 -0.1494530494156905 1106 58-59 -0.319631094626871 1106 60-61 -0.25399029561177144 1106 62-63 -0.27352997181737493 1106 64-65 -0.3312585650664417 1106 66-67 -0.2238541147228723 1106 68-69 -0.4627836122953859 1106 70-71 -0.239106328500732 1106 72-73 -0.249522116036065 1106 74-75 -0.3206186278714753 1106 76-77 -0.4190444222812957 1106 78-79 -0.3372108453555569 1106 80-81 -0.16562593856668784 1106 82-83 0.07141398515879871 1106 84-85 0.3651866076043824 1106 86-87 0.4184272065243988 1106 88-89 0.22610483963906347 1106 90-91 0.35491357954752445 1106 92-93 0.35640021385806975 1106 94-95 0.46064893668870255 1106 96-97 0.46260577694873106 1106 98-99 0.6178059441791568 1106 100-101 1.501862040480873 1107 1 0.11055899296196614 1107 2 0.09935541564469474 1107 3 0.08708697238688856 1107 4 0.03056428106508946 1107 5 -0.10270555439037565 1107 6 -0.06618774358631896 1107 7 -0.02382037985024965 1107 8 -0.00245932913271929 1107 9 0.014180908819575677 1107 10-11 -0.12068406974786683 1107 12-13 -0.04425087086703883 1107 14-15 0.044068317199410956 1107 16-17 -0.019936837704214838 1107 18-19 -0.09160217426695283 1107 20-21 -0.12799640086849706 1107 22-23 -0.22122683316872127 1107 24-25 -0.17416836399294056 1107 26-27 -0.05399456772682498 1107 28-29 -0.03201781050935182 1107 30-31 -0.022871500578730775 1107 32-33 0.025676435004037046 1107 34-35 0.017430504615127518 1107 36-37 0.01493440332840379 1107 38-39 -0.1271704859130196 1107 40-41 -0.046129568757827144 1107 42-43 -0.13938557160661702 1107 44-45 -0.09247321874080683 1107 46-47 -0.2203348669648051 1107 48-49 -0.028444174810346112 1107 50-51 -0.10149264411478498 1107 52-53 0.1112215232828433 1107 54-55 -0.006886667907352262 1107 56-57 -0.2856197993081153 1107 58-59 -0.10322862547794642 1107 60-61 -0.23733894602639793 1107 62-63 -0.1509390919440179 1107 64-65 -0.02158403533625375 1107 66-67 -0.06725898236735617 1107 68-69 -0.06499093208947926 1107 70-71 -0.20625782948615878 1107 72-73 -0.16381972604015616 1107 74-75 -0.17109547163193284 1107 76-77 -0.0764539939519544 1107 78-79 0.23709470651091635 1107 80-81 0.3904764131807852 1107 82-83 0.29891580461556444 1107 84-85 0.44144641540600205 1107 86-87 0.208454591340832 1107 88-89 -0.21225110188332508 1107 90-91 0.05533172258604324 1107 92-93 0.08858115816940781 1107 94-95 0.15359269820971377 1107 96-97 0.6782821234795442 1107 98-99 0.6868330793183155 1107 100-101 1.2462934403204429 1108 1 -0.03820309962124924 1108 2 0.052493490958500644 1108 3 0.004755469042272864 1108 4 0.09977050125004183 1108 5 -0.02895745322575749 1108 6 0.06323221217414954 1108 7 0.011818428347389442 1108 8 0.07403775265711232 1108 9 -0.031287870329208545 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2316 52-53 -1.2338813898038978 2316 54-55 -1.0744152104924538 2316 56-57 -0.9670190940956402 2316 58-59 -1.684082942090022 2316 60-61 -1.251646552298503 2316 62-63 -1.0761700472127806 2316 64-65 -0.9412485510201449 2316 66-67 -0.9760012384662424 2316 68-69 -1.41040387357355 2316 70-71 -1.3191136131725827 2316 72-73 -0.6368831152724113 2316 74-75 -0.5452870374069789 2316 76-77 -0.8915790220950868 2316 78-79 -0.9721013803224565 2316 80-81 -0.7937258469852395 2316 82-83 -1.0082138498956894 2316 84-85 -0.8451678021422389 2316 86-87 -0.9741262611600945 2316 88-89 -1.0216562649682501 2316 90-91 -0.7649730040079454 2316 92-93 -0.929530764351636 2316 94-95 -1.4171968165663387 2316 96-97 -1.2678732520406477 2316 98-99 -2.1447774705440565 2316 100-101 -3.1649122478608938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 30.0 5 52.0 6 126.0 7 306.0 8 678.0 9 1314.0 10 2121.0 11 3448.0 12 5054.0 13 6473.0 14 7497.0 15 9256.0 16 11476.0 17 13945.0 18 12796.0 19 8800.0 20 7890.0 21 8304.0 22 8687.0 23 9840.0 24 11569.0 25 13645.0 26 16297.0 27 20150.0 28 24822.0 29 30407.0 30 36657.0 31 44598.0 32 54828.0 33 68319.0 34 87873.0 35 115764.0 36 159209.0 37 201455.0 38 120150.0 39 6746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30675835516287 18.880805839612368 14.608497021716301 22.203938783508455 2 16.179089267753724 20.93780104082169 39.47488307784578 23.408226613578805 3 19.3336688813425 26.0086564309781 29.9276684691315 24.730006218547903 4 13.127039864141732 17.296721180976306 36.3823313491186 33.193907605763364 5 13.522267922692494 36.70638184954226 33.54358498076158 16.22776524700367 6 30.500181321257042 35.77662990120203 18.643982345501023 15.079206432039907 7 28.256043304823148 32.84515164648546 20.892985078587287 18.005819970104106 8 27.14538426839084 33.300046878178655 20.19644610336196 19.35812275006855 9 26.85332437046144 16.887377387028014 20.759072696556665 35.50022554595388 10-11 25.037325644132707 26.52252363810046 27.220831601199375 21.219319116567455 12-13 26.231184403022173 24.052520038422827 27.966093449545276 21.750202109009727 14-15 22.853475996371806 25.19252124665938 27.13717047611003 24.81683228085879 16-17 21.79628415087881 28.344637907923254 27.657341590382718 22.201736350815217 18-19 21.583331969716202 26.326375642761207 30.783634648494147 21.30665773902845 20-21 22.379642487550747 25.484304655091588 30.914389831857704 21.22166302549996 22-23 21.946505806702696 25.53954130144438 30.940880425264684 21.573072466588243 24-25 22.015319434985273 25.62449694407345 31.057014479165744 21.303169141775534 26-27 21.67184839572784 25.88864073590872 30.855140083585788 21.584370784777658 28-29 21.480893769064107 26.14499622090105 30.99825263663466 21.375857373400184 30-31 21.65593320932586 25.667468911891895 30.969319206033735 21.70727867274852 32-33 21.51650555256527 25.891597048597824 30.78437364967336 21.807523749163543 34-35 22.17764297863646 25.95299189524585 30.433205875615066 21.436159250502623 36-37 22.26872713297667 25.686557982567436 30.339501081519266 21.705213802936623 38-39 21.877846985004183 25.96171706253947 30.39938721826457 21.76104873419177 40-41 22.16278417709707 25.82560948319858 30.373371755783936 21.638234583920415 42-43 22.06956211768941 26.000370618202663 29.869704261711295 22.060363002396635 44-45 22.06615645543981 26.209039185725878 29.710037569295338 22.014766789538974 46-47 22.382301237188628 26.532002167014934 29.070638053224542 22.015058542571893 48-49 22.57078301464153 26.61797802996766 28.67673431155804 22.134504643832777 50-51 22.46332554830909 26.87916348064439 28.553068996306795 22.10444197473973 52-53 22.84417870315499 26.975207634951666 28.16038528555887 22.02022837633448 54-55 22.301625981023882 26.973789536158023 28.35865516695428 22.36592931586382 56-57 22.49046058553229 27.23347470877529 28.140775086548043 22.13528961914438 58-59 22.706124138956078 26.82564313987087 28.212912518114436 22.255320203058616 60-61 22.55013150479884 26.92765334043421 28.621512999645248 21.9007021551217 62-63 22.484354688683847 26.937490849886835 28.56763741534901 22.010517046080302 64-65 22.274405452493923 26.989075284949777 28.327871491841293 22.408647770715003 66-67 22.26937137186188 27.20049921588433 28.10021997694974 22.429909435304047 68-69 22.299807356539 26.941559509847107 28.3712605675287 22.387372566085197 70-71 22.437277190350695 26.99481111402503 28.076105429954907 22.49180626566937 72-73 22.471143385312093 27.052247056846422 28.05106183497112 22.42554772287036 74-75 22.31303167200688 27.466526914461483 27.751382595985664 22.469058817545978 76-77 22.381699793864403 27.291804595306868 27.83914082011098 22.487354790717752 78-79 22.485422482636125 27.37720564560357 27.71692018101597 22.42045169074434 80-81 22.563500565717433 27.507977102376717 27.51470024937568 22.413822082530167 82-83 22.60958796561478 27.511538776259425 27.58332577719491 22.295547480930885 84-85 22.39706829554865 27.499769965247054 27.49149756163162 22.611664177572674 86-87 22.5526212200958 27.297000844770164 27.440656001627623 22.709721933506415 88-89 22.47146172761291 27.33661667620818 27.353782554335755 22.838139041843156 90-91 22.432354466313065 27.322309354542412 27.546363892177734 22.698972286966786 92-93 22.554963418178506 27.212239150754968 27.524243004686028 22.708554426380495 94-95 22.238358976173835 27.12316526139772 27.64892459323878 22.989551169189664 96-97 22.337826794312992 27.131776085914822 27.613303665016442 22.917093454755744 98-99 22.062529866728024 27.2128369409391 27.310268844799207 23.414364347533674 100-101 21.944755674265394 27.31776240213961 27.436885247352482 23.300596676242517 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 405.0 1 330.0 2 820.0 3 1147.0 4 730.0 5 527.5 6 626.5 7 1033.5 8 1555.5 9 1852.0 10 1714.0 11 1527.0 12 1527.0 13 1557.0 14 1733.5 15 1928.0 16 2047.0 17 2218.0 18 2407.0 19 2562.5 20 2861.0 21 3210.0 22 3592.5 23 4094.5 24 4711.0 25 5667.0 26 6956.0 27 8402.0 28 9886.5 29 11639.0 30 13706.5 31 16130.0 32 18853.5 33 20801.5 34 22363.0 35 23922.0 36 26040.0 37 28271.5 38 29100.5 39 29446.5 40 30389.5 41 32538.0 42 34594.5 43 36227.5 44 37676.5 45 39163.5 46 39499.0 47 39892.5 48 43207.5 49 44024.5 50 41656.5 51 39709.0 52 38016.5 53 37750.5 54 37549.0 55 35108.0 56 31714.5 57 28736.5 58 26599.0 59 23842.0 60 19860.0 61 16335.0 62 13653.0 63 10604.0 64 7818.5 65 6095.0 66 4569.5 67 3677.5 68 2967.5 69 2135.0 70 1633.5 71 1263.5 72 1045.5 73 928.5 74 742.0 75 590.5 76 404.0 77 226.5 78 142.0 79 102.0 80 71.0 81 47.5 82 33.0 83 20.5 84 13.0 85 6.5 86 4.0 87 3.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021316303876736924 2 0.012117566934078667 3 0.00893338876162004 4 0.0 5 0.0035379757471762532 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010613927241528759 14-15 4.4224696839703164E-5 16-17 4.4224696839703164E-5 18-19 0.001636313783069017 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001326740905191095 28-29 0.002609257113542487 30-31 0.0011498421178322822 32-33 0.006766378616474584 34-35 0.0016805384799087203 36-37 3.9802227155732843E-4 38-39 7.075951494352506E-4 40-41 8.844939367940633E-5 42-43 0.004112896806092394 44-45 0.001105617420992579 46-47 2.2112348419851583E-4 48-49 3.9802227155732843E-4 50-51 4.4224696839703164E-5 52-53 0.0 54-55 9.729433304734696E-4 56-57 0.013134734961391838 58-59 0.024412032655516145 60-61 0.01883972085371355 62-63 0.012338690418277183 64-65 0.015169071016018186 66-67 0.0015036396925499076 68-69 3.537975747176253E-4 70-71 4.4224696839703164E-5 72-73 0.0 74-75 0.0015478643893896108 76-77 0.002078560751466049 78-79 0.007252850281711319 80-81 0.014771048744460856 82-83 0.014373026472903528 84-85 0.02883450233948646 86-87 0.009287186336337664 88-89 0.03900618261261819 90-91 0.044932291989138416 92-93 0.027905783705852696 94-95 0.028303805977410026 96-97 0.04780689728371912 98-99 0.0504161543972616 100-101 0.05873039740312581 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1130590.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.46725522790524 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75950306146169 53.79453013792204 2 9.290807991920696 11.793241641933852 3 2.353925983996467 4.481916636417052 4 1.0342659425606684 2.6256808220011174 5 0.5485193057444653 1.7406507387558685 6 0.3678299346930143 1.4007093807375155 7 0.2606441868845379 1.1579659792869559 8 0.18957621512602518 0.9625505624434969 9 0.1417846965944896 0.8098816973556222 >10 0.9355132479743249 11.294538097737744 >50 0.08035075808438476 3.454856974629951 >100 0.03518436927670125 4.252373622615751 >500 0.0011169630306697902 0.4435091770337611 >1k 6.981018941686188E-4 0.8295969860919223 >5k 2.7924075766744754E-4 0.9579975450373609 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5802 0.5131833821279156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5009 0.44304301294014625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3128 0.276669703429183 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.207236929390849 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1512 0.13373548324326237 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1363 0.12055652358503083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.844939367940633E-5 0.0 0.0 0.0 0.0 7 8.844939367940633E-5 0.0 0.0 0.0 0.0 8 8.844939367940633E-5 0.0 0.0 0.0 0.0 9 8.844939367940633E-5 0.0 0.0 0.0 0.0 10-11 8.844939367940633E-5 0.0 0.0 0.0 0.0 12-13 1.7689878735881266E-4 0.0 0.0 0.0 0.0 14-15 2.211234841985158E-4 0.0 0.0 0.0 8.844939367940633E-5 16-17 3.0957287787792214E-4 0.0 0.0 0.0 1.7689878735881266E-4 18-19 3.537975747176253E-4 0.0 0.0 0.0 1.7689878735881266E-4 20-21 3.537975747176253E-4 0.0 0.0 0.0 1.7689878735881266E-4 22-23 3.537975747176253E-4 0.0 0.0 0.0 1.7689878735881266E-4 24-25 3.537975747176253E-4 0.0 0.0 0.0 1.7689878735881266E-4 26-27 3.537975747176253E-4 0.0 0.0 2.6534818103821897E-4 1.7689878735881266E-4 28-29 3.537975747176253E-4 0.0 0.0 0.0010171680273131727 1.7689878735881266E-4 30-31 3.537975747176253E-4 0.0 0.0 0.002609257113542487 1.7689878735881266E-4 32-33 4.422469683970316E-4 0.0 0.0 0.0048647166523673475 1.7689878735881266E-4 34-35 5.306963620764379E-4 0.0 0.0 0.007297074978551022 1.7689878735881266E-4 36-37 6.191457557558443E-4 0.0 0.0 0.009640983911055289 1.7689878735881266E-4 38-39 6.191457557558443E-4 0.0 0.0 0.012825162083513917 1.7689878735881266E-4 40-41 6.191457557558443E-4 0.0 0.0 0.01631891313385047 1.7689878735881266E-4 42-43 7.075951494352506E-4 0.0 0.0 0.020829832211500192 1.7689878735881266E-4 44-45 8.844939367940633E-4 0.0 0.0 0.022598820085088317 1.7689878735881266E-4 46-47 0.0011940668146719854 0.0 0.0 0.025517650076508726 1.7689878735881266E-4 48-49 0.0012382915115116886 0.0 0.0 0.03091306309095251 1.7689878735881266E-4 50-51 0.0012382915115116886 0.0 0.0 0.037590992313747684 1.7689878735881266E-4 52-53 0.0012382915115116886 0.0 0.0 0.04979700864150577 1.7689878735881266E-4 54-55 0.0012382915115116886 0.0 0.0 0.059216869068362536 1.7689878735881266E-4 56-57 0.0012382915115116886 0.0 0.0 0.07916220734306867 1.7689878735881266E-4 58-59 0.0012382915115116886 0.0 0.0 0.11259607815388425 1.7689878735881266E-4 60-61 0.0012382915115116886 0.0 0.0 0.1412536817060119 1.7689878735881266E-4 62-63 0.0012382915115116886 0.0 0.0 0.15726302196198444 1.7689878735881266E-4 64-65 0.0012382915115116886 0.0 0.0 0.18830875914345607 1.7689878735881266E-4 66-67 0.0012382915115116886 0.0 0.0 0.24796787518021562 1.7689878735881266E-4 68-69 0.0012382915115116886 0.0 0.0 0.3273069813106431 1.7689878735881266E-4 70-71 0.0012382915115116886 0.0 0.0 0.40067575336771066 1.7689878735881266E-4 72-73 0.0012382915115116886 0.0 0.0 0.4810320275254513 1.7689878735881266E-4 74-75 0.0012382915115116886 0.0 0.0 0.5718695548342017 1.7689878735881266E-4 76-77 0.0012382915115116886 0.0 0.0 0.6417003511440929 1.7689878735881266E-4 78-79 0.0012825162083513917 0.0 0.0 0.724223635446979 1.7689878735881266E-4 80-81 0.0014151902988705012 0.0 0.0 0.834918051636756 1.7689878735881266E-4 82-83 0.0015036396925499076 0.0 0.0 0.9372540001238292 1.7689878735881266E-4 84-85 0.0015478643893896108 0.0 0.0 1.079701748644513 1.7689878735881266E-4 86-87 0.001592089086229314 0.0 0.0 1.2959605161906615 2.211234841985158E-4 88-89 0.001592089086229314 0.0 0.0 1.549898725444237 2.6534818103821897E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1720 0.0 52.748127 2 GTACCTG 1880 0.0 49.537586 1 TATAACG 95 1.9279469E-8 40.000782 2 GGTATCA 4830 0.0 33.644615 1 ACCTGGG 2700 0.0 32.541172 3 GTATCAA 7080 0.0 29.932173 1 ATAACGC 160 1.9081199E-9 29.682829 3 TAACGCA 130 3.0445153E-7 29.222292 4 TATCACG 100 4.952377E-5 28.500557 2 GTACATG 4760 0.0 26.652681 1 CCTGGGG 1750 0.0 26.592283 4 GTACACG 205 9.531504E-10 25.4962 1 GCACCGT 325 0.0 24.838947 6 TACATGG 5010 0.0 24.745993 2 ATCAACG 8610 0.0 24.380602 3 TCAACGC 8720 0.0 24.124306 4 TATCAAC 8755 0.0 24.089561 2 ACATGGG 4950 0.0 24.082067 3 CAACGCA 8920 0.0 23.743113 5 AACGCAG 9110 0.0 23.456423 6 >>END_MODULE