FastQCFastQC Report
Thu 26 May 2016
SRR936719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208312
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72690.6015830348453048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.4877051622428645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33380.27625315315911786No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20260.16767192579400023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15960.1320850906057376No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13970.11561583432093697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70900.030.4263321
GTATCAA102600.029.6859281
GTACATG84400.025.7284561
TACATGG85550.024.7150782
ATCAACG124500.024.3124123
TCAACGC125150.024.2241084
TATCAAC125350.024.1834532
CAACGCA126700.023.926775
AACGCAG129600.023.4296
GAGTACT61300.023.25127412-13
ACATGGG86950.023.0622043
TACCTGG8450.023.054152
TAAGGTG5350.022.2037435
GTACTTT64600.021.846514-15
CATGGGG31600.021.8041344
ACGCAGA143250.021.2287927
GTCTAGG3600.021.1182251
GTATAGG4300.020.9954451
CGCAGAG144350.020.9353528
AGTACTT63400.020.4952912-13
Produced by FastQC (version 0.11.5)