##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936719_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1208312 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50539761253716 31.0 31.0 34.0 30.0 34.0 2 31.564728315203357 33.0 31.0 34.0 30.0 34.0 3 31.558608207151796 33.0 31.0 34.0 28.0 34.0 4 35.22437913386609 37.0 35.0 37.0 33.0 37.0 5 35.23781771595416 37.0 35.0 37.0 33.0 37.0 6 35.250252418249595 37.0 35.0 37.0 32.0 37.0 7 35.1811750607459 37.0 35.0 37.0 32.0 37.0 8 35.14361274240428 37.0 35.0 37.0 32.0 37.0 9 36.64209988810837 39.0 37.0 39.0 32.0 39.0 10-11 36.525137961056416 39.0 37.0 39.0 32.0 39.0 12-13 36.421258333940244 39.0 37.0 39.0 32.0 39.0 14-15 37.48805565118943 40.0 37.0 41.0 32.0 41.0 16-17 37.4035278140083 40.0 37.0 41.0 32.0 41.0 18-19 37.411940790127055 40.0 37.0 41.0 32.0 41.0 20-21 37.30307155767716 39.5 37.0 41.0 31.5 41.0 22-23 37.390608551433736 40.0 37.0 41.0 32.0 41.0 24-25 37.40494590801052 40.0 37.0 41.0 32.0 41.0 26-27 37.30776239911546 40.0 37.0 41.0 31.0 41.0 28-29 37.16145581604751 39.5 36.0 41.0 31.0 41.0 30-31 36.91720929693655 39.0 36.0 41.0 30.5 41.0 32-33 36.65451224518171 39.0 35.0 41.0 30.0 41.0 34-35 36.43702826753355 39.0 35.0 41.0 30.0 41.0 36-37 36.21115779699283 39.0 35.0 40.0 30.0 41.0 38-39 35.99159447228861 38.0 35.0 40.0 29.5 41.0 40-41 35.741973099663 38.0 35.0 40.0 28.5 41.0 42-43 35.460695581935795 38.0 35.0 40.0 27.0 41.0 44-45 35.11401773714074 38.0 34.5 40.0 26.0 41.0 46-47 34.80809468084402 38.0 34.0 40.0 24.5 41.0 48-49 34.56300938830368 38.0 34.0 40.0 23.5 41.0 50-51 33.552145472361445 37.5 32.5 39.5 22.0 40.5 52-53 33.64163850065215 37.5 33.0 39.5 22.0 40.5 54-55 34.065457845324715 38.0 34.0 40.0 22.0 41.0 56-57 33.956288193777766 38.0 33.5 40.0 21.5 41.0 58-59 33.71220885003211 38.0 33.0 40.0 20.5 41.0 60-61 33.429834347420204 37.0 33.0 40.0 19.5 41.0 62-63 33.06294855964353 37.0 33.0 40.0 18.0 41.0 64-65 32.70361793973741 36.0 32.0 39.0 16.0 41.0 66-67 32.2451606042148 36.0 31.5 39.0 12.5 41.0 68-69 31.863117721250802 35.0 31.0 39.0 9.0 40.0 70-71 31.458326160792907 35.0 31.0 38.0 7.5 40.0 72-73 31.070295999708684 35.0 31.0 37.0 7.0 39.5 74-75 30.650048993968447 35.0 30.0 37.0 2.0 39.0 76-77 30.18782690232324 34.5 30.0 36.0 2.0 39.0 78-79 29.71562642761141 34.0 29.0 36.0 2.0 38.5 80-81 29.286498437489655 34.0 29.0 35.0 2.0 37.0 82-83 28.87977773952423 34.0 29.0 35.0 2.0 37.0 84-85 28.529122031395865 34.0 28.5 35.0 2.0 36.0 86-87 28.175543650977563 34.0 27.5 35.0 2.0 36.0 88-89 27.771779143135216 34.0 27.0 35.0 2.0 36.0 90-91 27.373333625752288 33.5 26.0 35.0 2.0 35.0 92-93 26.92953310072233 33.0 25.5 35.0 2.0 35.0 94-95 26.434163113500485 33.0 25.0 35.0 2.0 35.0 96-97 25.86320875734082 33.0 23.5 35.0 2.0 35.0 98-99 25.138508100556813 32.5 18.5 35.0 2.0 35.0 100-101 22.36891340978158 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29437474298796573 1101 2 0.26700815476584694 1101 3 0.3024804775166601 1101 4 0.2810977428033752 1101 5 0.20593310730105685 1101 6 0.39719899559553085 1101 7 0.36721958743586214 1101 8 0.3526677017508604 1101 9 0.3755031794537942 1101 10-11 0.547442615316001 1101 12-13 0.5037583486640713 1101 14-15 0.6544210339599417 1101 16-17 0.5943720676884467 1101 18-19 0.5434849468972942 1101 20-21 0.5773152903624847 1101 22-23 0.3852733546020062 1101 24-25 0.43172839453782075 1101 26-27 0.4499000713631034 1101 28-29 0.5125226964532672 1101 30-31 0.5042496409663286 1101 32-33 0.25293854156940654 1101 34-35 0.12361637217108523 1101 36-37 0.093632083256324 1101 38-39 0.3244379773501649 1101 40-41 0.2970019317299233 1101 42-43 0.2748012474694761 1101 44-45 0.20788279554114553 1101 46-47 0.4459448727090347 1101 48-49 0.4075456907302737 1101 50-51 0.3262077474380831 1101 52-53 0.47844029132672006 1101 54-55 0.3915926234714888 1101 56-57 0.4291092826577625 1101 58-59 0.49357650654299334 1101 60-61 0.2972273233720486 1101 62-63 0.3504201062046306 1101 64-65 0.41213114075703317 1101 66-67 0.36258742213732376 1101 68-69 0.2483760028372437 1101 70-71 0.13243628462337043 1101 72-73 0.0976941876637234 1101 74-75 0.30287020807875464 1101 76-77 0.22547563833287398 1101 78-79 0.17445747262139832 1101 80-81 0.1515282567629903 1101 82-83 0.01991673932025506 1101 84-85 -0.04338763639497145 1101 86-87 0.002392441751265295 1101 88-89 -0.12919764216006158 1101 90-91 0.009905266930996248 1101 92-93 -0.09234264522474689 1101 94-95 -0.10486961681721851 1101 96-97 -0.26699007539133035 1101 98-99 -0.3738390298719487 1101 100-101 -0.3118492263252044 1102 1 0.3004564338468292 1102 2 0.3163905476232358 1102 3 0.244821528929954 1102 4 0.2392657953258137 1102 5 0.27409378849452537 1102 6 0.2724278856938369 1102 7 0.26177238199623787 1102 8 0.3487078513819455 1102 9 0.511514532678234 1102 10-11 0.42705641279044926 1102 12-13 0.35280868726297143 1102 14-15 0.34378909750493136 1102 16-17 0.20808205067238816 1102 18-19 0.3172278597433831 1102 20-21 0.31709359936584036 1102 22-23 0.17324913255278318 1102 24-25 0.24237893154258217 1102 26-27 -0.06697200507826295 1102 28-29 -0.12123799025147974 1102 30-31 0.05436158871945196 1102 32-33 0.018588148546676564 1102 34-35 0.12500314649130218 1102 36-37 -0.17055221374622676 1102 38-39 -0.033031521192789626 1102 40-41 0.05703761722907075 1102 42-43 -0.03071691562289658 1102 44-45 0.03173421159410594 1102 46-47 0.0639343599538833 1102 48-49 0.13878841412632426 1102 50-51 0.3571425283993932 1102 52-53 0.4190914483498034 1102 54-55 0.36304422415213367 1102 56-57 0.2284451741407878 1102 58-59 0.3556107177763508 1102 60-61 0.2975428438085359 1102 62-63 0.1836475771498769 1102 64-65 0.13575797239449372 1102 66-67 0.1911509774601825 1102 68-69 0.3441667673198374 1102 70-71 0.3230409402720795 1102 72-73 -0.03357678101157191 1102 74-75 0.11675104055669294 1102 76-77 -0.0391681611394219 1102 78-79 0.08413664758368 1102 80-81 0.2607768634440326 1102 82-83 0.2733568076600541 1102 84-85 -0.08632046085400447 1102 86-87 0.0030393275369995365 1102 88-89 -0.08008736845641451 1102 90-91 -0.11339502172197413 1102 92-93 -0.21272471429479012 1102 94-95 -0.29328974123422924 1102 96-97 -0.07829232054657709 1102 98-99 -0.321512927789378 1102 100-101 -0.21350123070666527 1103 1 0.27014322032131943 1103 2 0.21880819048590183 1103 3 0.11941178444550005 1103 4 0.31052710072117407 1103 5 0.32219282046333575 1103 6 0.22539629504583303 1103 7 0.2493303593009344 1103 8 0.3079938173647747 1103 9 0.15406264059122066 1103 10-11 0.3755608203547922 1103 12-13 0.02333974361733482 1103 14-15 0.192768782887633 1103 16-17 0.36209927985140666 1103 18-19 0.536772836380564 1103 20-21 0.4340805557256502 1103 22-23 0.14237512557974696 1103 24-25 0.13572790693069692 1103 26-27 0.03362703405265677 1103 28-29 0.19668978127139525 1103 30-31 -6.706551132538152E-4 1103 32-33 -0.026889406823620732 1103 34-35 0.07221407644870226 1103 36-37 0.15685318190321595 1103 38-39 0.19049272542454077 1103 40-41 0.09803441146729597 1103 42-43 -0.0016470449133620946 1103 44-45 0.15729880096152016 1103 46-47 0.24043661315462117 1103 48-49 0.11437148174526612 1103 50-51 0.21402975949785485 1103 52-53 0.3057876802189128 1103 54-55 0.3392244687526187 1103 56-57 0.2902963225139814 1103 58-59 0.17183390640635565 1103 60-61 0.1584283961077233 1103 62-63 0.24598259787703824 1103 64-65 0.3600252166139555 1103 66-67 0.2875694912357858 1103 68-69 0.3023585056792548 1103 70-71 0.1848601456213217 1103 72-73 -0.11364863132913783 1103 74-75 0.03622267715680749 1103 76-77 0.18009018773569707 1103 78-79 0.12776823995058706 1103 80-81 0.16944101477661633 1103 82-83 -0.15341030047925486 1103 84-85 -0.1529183712359199 1103 86-87 -0.010827239433055524 1103 88-89 -0.08573483779541036 1103 90-91 -0.09314156886730274 1103 92-93 -0.2823685280913111 1103 94-95 -0.15388150365834719 1103 96-97 -0.09572232521627555 1103 98-99 -0.05342558438897527 1103 100-101 -0.21427946590472757 1104 1 0.30780937723675095 1104 2 0.3069939158408559 1104 3 0.25191799529669723 1104 4 0.4337506362393171 1104 5 0.38222082890922593 1104 6 0.41746473921445215 1104 7 0.45805707751255653 1104 8 0.4186222235776711 1104 9 0.2948450313183315 1104 10-11 0.4916422641090392 1104 12-13 0.5523026568921168 1104 14-15 0.34367094347723537 1104 16-17 0.3291075975298696 1104 18-19 0.49640385204907034 1104 20-21 0.3191448134925423 1104 22-23 0.3350361633044372 1104 24-25 0.4273817988473283 1104 26-27 0.1928961139547738 1104 28-29 0.23516407661617933 1104 30-31 0.3312566814891724 1104 32-33 0.3486822051733114 1104 34-35 0.30669469072681466 1104 36-37 0.01456940964495601 1104 38-39 0.09495818962334823 1104 40-41 0.02205484363476984 1104 42-43 0.30452113087710586 1104 44-45 0.3228327731252634 1104 46-47 0.10537421353318877 1104 48-49 0.29391772881790956 1104 50-51 0.18327187770935893 1104 52-53 0.2500745952205321 1104 54-55 0.23317639109699684 1104 56-57 0.17891319955368346 1104 58-59 0.31219836925055944 1104 60-61 0.3853294802117233 1104 62-63 0.43640131856866304 1104 64-65 0.3830724141280015 1104 66-67 0.4341426374414823 1104 68-69 0.3628028935534964 1104 70-71 0.38485280594675686 1104 72-73 0.23945475030977903 1104 74-75 0.375382380423364 1104 76-77 0.25776314546350676 1104 78-79 0.24734423593204014 1104 80-81 0.27663463910791464 1104 82-83 0.22395457673139418 1104 84-85 0.037503756055716764 1104 86-87 0.05851301982778523 1104 88-89 0.031993542217634285 1104 90-91 0.15323226026879055 1104 92-93 -0.08980674630324259 1104 94-95 -0.08319517285766409 1104 96-97 0.19884916476243575 1104 98-99 0.21431242701136455 1104 100-101 0.12240209070088426 1105 1 0.17537257166367937 1105 2 0.19520389972933927 1105 3 0.18347324556766864 1105 4 0.14422049616275956 1105 5 0.031233700501161366 1105 6 0.22480375448048306 1105 7 0.2478638049604882 1105 8 0.4033935060343623 1105 9 0.4033578066834309 1105 10-11 0.4279753409526421 1105 12-13 0.09845560469847214 1105 14-15 0.14954686164883668 1105 16-17 0.10741962332764388 1105 18-19 0.09024764946065744 1105 20-21 0.05866878606806836 1105 22-23 -0.14669144875318807 1105 24-25 -0.057543239237425325 1105 26-27 -0.2052411279733235 1105 28-29 -0.2557933648625266 1105 30-31 -0.23463572481404782 1105 32-33 -0.3206778915441575 1105 34-35 -0.22708697559030355 1105 36-37 -0.12914563038506088 1105 38-39 -0.17910050632853114 1105 40-41 -0.0702102808226428 1105 42-43 -0.10068714235044496 1105 44-45 0.06065040374758013 1105 46-47 0.2891847479013805 1105 48-49 0.2303836574345297 1105 50-51 0.2271784385138318 1105 52-53 0.230559032559583 1105 54-55 0.06717732330528037 1105 56-57 -0.027227489264618043 1105 58-59 0.08377866268541112 1105 60-61 0.06015429381325532 1105 62-63 0.02398277018073003 1105 64-65 -0.02620831013356195 1105 66-67 0.11061211798586612 1105 68-69 0.512827960903639 1105 70-71 0.21231808740766311 1105 72-73 -0.06173162120596842 1105 74-75 0.1386152980867088 1105 76-77 0.08568157412936017 1105 78-79 -0.03782909157815695 1105 80-81 0.09885996331633962 1105 82-83 -0.005795029636921356 1105 84-85 0.04484878333956743 1105 86-87 -0.10355474114129848 1105 88-89 0.163015796012548 1105 90-91 0.06015758651577485 1105 92-93 -0.1963626789727897 1105 94-95 0.1202418355346282 1105 96-97 0.09672018486710598 1105 98-99 0.17890786140210935 1105 100-101 0.32920271940592016 1106 1 0.2082984685369098 1106 2 0.11242744035548569 1106 3 0.04044517514834922 1106 4 0.2679408444861764 1106 5 0.21529998918859405 1106 6 0.31440667769641806 1106 7 0.46906109224787684 1106 8 0.4540930324018859 1106 9 0.5214035017912124 1106 10-11 0.4731714962882094 1106 12-13 0.3618503679318792 1106 14-15 0.3677553989486384 1106 16-17 0.5008051522172394 1106 18-19 0.2236516470378831 1106 20-21 0.1761956699238496 1106 22-23 0.373559566528165 1106 24-25 0.3420729893771295 1106 26-27 0.28632880976932995 1106 28-29 0.36393288152564907 1106 30-31 0.3436898801347681 1106 32-33 0.05978203835426399 1106 34-35 -0.06323446692690027 1106 36-37 -0.020870466798164955 1106 38-39 -0.018504841505425418 1106 40-41 0.10483762854178025 1106 42-43 0.16719322821483473 1106 44-45 -0.07013471406477123 1106 46-47 -0.04736736656477092 1106 48-49 -0.0739823006038236 1106 50-51 -0.31406829719526996 1106 52-53 -0.3887675554000225 1106 54-55 -0.25306823080499186 1106 56-57 -0.11628926007416851 1106 58-59 -0.029215449179240238 1106 60-61 -0.14773759485829174 1106 62-63 -0.17688654896017653 1106 64-65 0.12386715332127807 1106 66-67 -0.0339103274272432 1106 68-69 0.09764243002816997 1106 70-71 -0.08460578386756978 1106 72-73 -0.1963172176154977 1106 74-75 -0.039230220277719496 1106 76-77 -0.06443608928377031 1106 78-79 -0.07625091243189175 1106 80-81 0.12544345469265394 1106 82-83 0.06447783032872323 1106 84-85 0.26352648488160213 1106 86-87 0.27852119892483174 1106 88-89 0.13768589163268885 1106 90-91 0.00783460292907634 1106 92-93 0.12202359462195034 1106 94-95 0.1483470077601048 1106 96-97 0.09090900237462662 1106 98-99 0.1606650676655761 1106 100-101 0.04057982816265948 1107 1 0.27374170066675063 1107 2 0.22482138839619736 1107 3 0.27900361324011413 1107 4 0.28020894015740794 1107 5 0.33675965911063344 1107 6 0.4060897941491888 1107 7 0.26424807589962285 1107 8 0.38486418777517173 1107 9 0.48401472089399533 1107 10-11 0.5702098235227737 1107 12-13 0.4728943735851985 1107 14-15 0.09356542713990024 1107 16-17 0.36855954094207277 1107 18-19 0.23356691425242104 1107 20-21 0.29937890645518195 1107 22-23 0.351210729532923 1107 24-25 0.2886152289970312 1107 26-27 0.45292546547690904 1107 28-29 0.5751500178654396 1107 30-31 0.36717444271828725 1107 32-33 0.035607756252495903 1107 34-35 0.2704046413622052 1107 36-37 0.3233357637309098 1107 38-39 0.23004124564901218 1107 40-41 0.23386956943802062 1107 42-43 0.22661203126173035 1107 44-45 0.3655789081990477 1107 46-47 0.3655451367509883 1107 48-49 0.35895989182144206 1107 50-51 0.23083731771153992 1107 52-53 0.36151423173708963 1107 54-55 0.25415133555660674 1107 56-57 0.2278663450004359 1107 58-59 0.38609046663523117 1107 60-61 0.40843992392542816 1107 62-63 0.5228206183489945 1107 64-65 0.47348884310689954 1107 66-67 0.7419569786542581 1107 68-69 0.6100743262401274 1107 70-71 0.4752330849338442 1107 72-73 0.35457998258767276 1107 74-75 0.5957071171246149 1107 76-77 0.37836216786173793 1107 78-79 0.2407995732795989 1107 80-81 0.2543369077754143 1107 82-83 0.40610530062078354 1107 84-85 0.1374370962278988 1107 86-87 0.1993448708852803 1107 88-89 -0.04259846443244797 1107 90-91 -0.15575004743312704 1107 92-93 0.19217624919485132 1107 94-95 0.03093319027449226 1107 96-97 0.010699245636214272 1107 98-99 0.2985050008567143 1107 100-101 0.17556737262728817 1108 1 0.14344634628969644 1108 2 0.25776230827072766 1108 3 0.14673702265112354 1108 4 0.3356138712176957 1108 5 0.28351193912840955 1108 6 0.19281264924312325 1108 7 0.35159248625949147 1108 8 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sequence quality scores pass #Quality Count 2 2324.0 3 1027.0 4 1030.0 5 1068.0 6 1313.0 7 1572.0 8 1965.0 9 2460.0 10 3125.0 11 4025.0 12 5405.0 13 6851.0 14 8100.0 15 10148.0 16 13061.0 17 17025.0 18 13043.0 19 8850.0 20 8642.0 21 8717.0 22 9684.0 23 10922.0 24 12070.0 25 14021.0 26 16273.0 27 18882.0 28 22692.0 29 27370.0 30 32826.0 31 40673.0 32 50893.0 33 65379.0 34 86890.0 35 117344.0 36 163062.0 37 211749.0 38 163910.0 39 23913.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.04843713270715 18.727507192732112 14.778622769756424 21.445432904804314 2 15.585048709226198 20.271977089696158 40.442231768182154 23.70074243289549 3 19.2964290059769 25.724516223551525 30.035980601762528 24.943074168709046 4 12.484645209139702 16.6041162428027 37.49697870691967 33.41425984113793 5 13.096012756001729 38.29946469167201 33.45917135661668 15.145351195709585 6 30.844166597664984 36.12138776186139 17.635257100273247 15.399188540200381 7 28.173613938906378 34.160649670099744 20.158759323447914 17.50697706754596 8 27.382044311405007 33.25118626220617 19.566621081526762 19.80014834486207 9 27.53362100083121 16.62819693412238 20.464269511108757 35.37391255393765 10-11 25.158706864540196 26.77013781572162 26.403771789981604 21.667383529756577 12-13 26.6662444475076 24.53933613074223 27.249279433332212 21.54513998841795 14-15 23.42376612286679 25.380510211304184 26.704281342654358 24.491442323174667 16-17 21.579672598265432 28.968418386121126 27.222548988361005 22.229360027252433 18-19 21.563658262505356 26.696260241342845 30.649713480390634 21.090368015761165 20-21 22.729320251360683 25.43279728600682 30.60852039510892 21.22936206752358 22-23 22.245912908310835 25.719490780842165 30.625366517031843 21.409229793815157 24-25 21.97479878338954 25.844737332147073 30.817860172560053 21.362603711903333 26-27 21.560944483594934 26.008472230210945 30.861031672170952 21.569551614023165 28-29 21.245639059133346 26.436063879223198 30.873040602526746 21.44525645911671 30-31 21.465651836927073 25.98459051139987 30.787817242918976 21.761940408754086 32-33 21.210240265366977 26.205775273961773 30.587177405405498 21.99680705526575 34-35 22.053285906289105 26.114281741801786 30.36169466164368 21.47073769026543 36-37 22.12053286016538 25.730535098830227 30.45403516234872 21.694896878655676 38-39 21.756673772999026 26.090860638642997 30.36682578671734 21.785639801640635 40-41 21.76475042735821 26.17675063757169 30.456291528024394 21.60220740704571 42-43 21.697921916911437 26.317806264623094 29.86769590085717 22.116575917608298 44-45 21.728652132676316 26.3197533050731 29.84633706697211 22.105257495278465 46-47 22.221192310031892 26.661474805750057 29.0774694891962 22.039863395021854 48-49 22.2134265991229 26.917213645914973 28.6796854762423 22.189674278719824 50-51 22.110906273212375 27.238143713442124 28.57807324092868 22.07287677241682 52-53 22.44753800755537 27.250938214212855 28.233161284288943 22.068362493942832 54-55 21.797534554841445 27.176217380275197 28.48706880676808 22.539179258115283 56-57 22.143457156926438 27.499265500018623 27.934958474888376 22.422318868166563 58-59 22.41352269252113 26.880311657226706 28.124962497429134 22.58120315282303 60-61 22.082759345569116 27.11000247044058 28.757128379734443 22.05010980425586 62-63 22.099598860223438 27.016809469778792 28.801041457179345 22.08255021281843 64-65 21.773871850342548 27.24169208793137 28.57819128598219 22.406244775743893 66-67 21.849997434042475 27.318839260143395 28.20293578649909 22.62822751931504 68-69 21.959591903477595 27.12869032809586 28.53744714057141 22.374270627855132 70-71 21.98969418747537 27.257822514134677 28.224561717293163 22.52792158109679 72-73 21.999853492405816 27.359672418952126 28.108640761309744 22.531833327332315 74-75 21.79774546047602 27.967946512947687 27.88590455399102 22.34840347258528 76-77 22.078955265581506 27.509090720945395 27.880791001376288 22.531163012096815 78-79 22.058438708502056 27.543826961137473 27.911211586714035 22.486522743646436 80-81 22.124477285933136 27.906894876542776 27.579174847933018 22.389452989591067 82-83 22.166886647312786 27.830546451797744 27.704851111468088 22.29771578942139 84-85 21.993265391262888 27.769748561363077 27.517117958294428 22.719868089079604 86-87 22.16063638696433 27.685978461512995 27.512106086567833 22.641279064954844 88-89 22.04550900979391 27.5770427711185 27.416784039356102 22.96066417973149 90-91 21.901660707625297 27.532013029995568 27.760025533426308 22.806300728952827 92-93 22.078730536632047 27.61880834250478 27.718807804428202 22.58365331643497 94-95 21.869865536550137 27.35538660828467 27.776034962980127 22.99871289218506 96-97 21.836021036973634 27.288838707567937 27.77858945328619 23.096550802172235 98-99 21.72603090233169 27.56533695581519 27.429990721599545 23.278641420253578 100-101 21.807119202898402 27.44171581742792 27.507309980872506 23.243854998801165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 311.0 1 243.5 2 247.0 3 319.0 4 306.5 5 313.5 6 515.0 7 1042.5 8 1716.5 9 2128.5 10 1976.0 11 1731.5 12 1816.0 13 1921.5 14 2071.0 15 2257.5 16 2321.0 17 2353.0 18 2517.0 19 2773.0 20 3112.0 21 3475.5 22 3895.0 23 4496.5 24 5220.5 25 6310.0 26 7898.5 27 9686.0 28 11267.5 29 12958.0 30 15210.5 31 17928.5 32 21259.0 33 23488.5 34 25322.5 35 27095.5 36 29551.0 37 32502.0 38 33430.0 39 33480.0 40 33850.0 41 35318.5 42 36504.5 43 37415.5 44 38388.0 45 40175.5 46 41333.0 47 42960.0 48 48904.0 49 48779.0 50 42584.0 51 39286.0 52 37679.0 53 37615.5 54 37591.5 55 35487.0 56 32478.0 57 29582.0 58 27814.0 59 25298.0 60 21341.0 61 18069.0 62 15487.5 63 12277.5 64 8767.5 65 6731.0 66 5089.0 67 3903.5 68 2915.0 69 1944.5 70 1445.5 71 1088.0 72 852.0 73 756.5 74 645.5 75 511.0 76 374.0 77 248.5 78 159.0 79 106.0 80 75.5 81 47.5 82 28.0 83 19.0 84 19.0 85 15.0 86 10.0 87 8.0 88 6.0 89 4.0 90 4.0 91 3.0 92 1.0 93 1.5 94 1.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012248492111309 2 0.010096729983646607 3 0.013324373175140194 4 0.018207218003297162 5 0.033931633551599255 6 0.050649170081899376 7 0.005627685564655487 8 0.026152185859281377 9 0.036083395679261644 10-11 0.04166970120300055 12-13 0.033807493428849505 14-15 0.029917769582690563 16-17 0.02867636835519303 18-19 0.023131442872370712 20-21 0.05338025278239395 22-23 0.012289872152225585 24-25 0.00306212302782725 26-27 9.517409410814427E-4 28-29 4.138004091658446E-5 30-31 0.002524182495911652 32-33 0.0019034818821628853 34-35 0.0 36-37 2.069002045829223E-4 38-39 0.0 40-41 0.0013655413502472872 42-43 0.002896602864160912 44-45 0.0014896814729970405 46-47 8.689808592482735E-4 48-49 7.448407364985202E-4 50-51 0.003269023232410172 52-53 0.00268970265957799 54-55 0.004262144214408199 56-57 5.793205728321824E-4 58-59 0.005089745032739889 60-61 0.002110382086745807 62-63 4.138004091658446E-4 64-65 4.965604909990135E-4 66-67 0.015393375220969419 68-69 0.04216626169399956 70-71 0.05329749270056078 72-73 0.015186475016386496 74-75 0.03256609220135197 76-77 0.028924648600692535 78-79 0.05975277908354796 80-81 0.08536702441091373 82-83 0.05213885155489642 84-85 0.018538258330629838 86-87 0.02002793980362688 88-89 0.021972801726706348 90-91 0.04001449956633717 92-93 0.025283205000033105 94-95 0.014648534484470899 96-97 0.020565880335542474 98-99 0.055325114705473424 100-101 0.07344957262693741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1208312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.5164500983233 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84907699473854 43.219043981789305 2 12.500550222213553 13.879723452262061 3 4.103874848871268 6.8349768977137755 4 1.7812917225278675 3.955639720970989 5 1.0115896690667325 2.8079933691361325 6 0.5761545968324436 1.9191634754380762 7 0.3759164000699323 1.4608680845936615 8 0.2741788126448769 1.2177147496173475 9 0.19706115122169848 0.9846122011305808 >10 1.1880338044904653 12.289343311980222 >50 0.0935326521507092 3.576320795258675 >100 0.04395492279166989 4.596245249987268 >500 0.0038871644340469076 1.4722647132522133 >1k 5.980252975456782E-4 0.6936360473925328 >5k 2.990126487728391E-4 1.092453949477147 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7269 0.6015830348453048 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.4877051622428645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3338 0.27625315315911786 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2026 0.16767192579400023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1596 0.1320850906057376 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1397 0.11561583432093697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.138004091658446E-5 0.0 14-15 0.0 0.0 0.0 8.276008183316892E-5 0.0 16-17 0.0 0.0 0.0 1.6552016366633783E-4 0.0 18-19 0.0 0.0 0.0 1.6552016366633783E-4 0.0 20-21 0.0 0.0 0.0 2.4828024549950677E-4 0.0 22-23 0.0 0.0 0.0 3.3104032733267567E-4 0.0 24-25 0.0 0.0 0.0 7.034606955819357E-4 0.0 26-27 0.0 0.0 0.0 9.10360900164858E-4 0.0 28-29 0.0 0.0 0.0 0.001448301432080456 0.0 30-31 0.0 0.0 0.0 0.0026483226186614054 0.0 32-33 0.0 0.0 0.0 0.005006984950906719 0.0 34-35 0.0 0.0 0.0 0.007158747078569111 0.0 36-37 0.0 0.0 0.0 0.010717430597395376 0.0 38-39 0.0 0.0 0.0 0.01568303550738551 0.0 40-41 0.0 0.0 0.0 0.02106244082654149 0.0 42-43 0.0 0.0 0.0 0.030621230278272498 0.0 44-45 0.0 0.0 0.0 0.03823515780692403 0.0 46-47 0.0 0.0 0.0 0.05362853302789346 0.0 48-49 0.0 0.0 0.0 0.07092539013102576 0.0 50-51 0.0 0.0 0.0 0.08180834089208747 0.0 52-53 0.0 0.0 0.0 0.09815345705413833 0.0 54-55 0.0 0.0 0.0 0.1262505048364992 0.0 56-57 0.0 0.0 0.0 0.1693271274306636 0.0 58-59 0.0 0.0 0.0 0.2279626454094638 0.0 60-61 0.0 0.0 0.0 0.2993018359496554 0.0 62-63 0.0 0.0 0.0 0.36923410509868315 0.0 64-65 8.276008183316892E-5 0.0 0.0 0.4403663954342918 0.0 66-67 8.276008183316892E-5 0.0 0.0 0.5410440349843418 0.0 68-69 8.276008183316892E-5 0.0 0.0 0.668287660802839 0.0 70-71 8.276008183316892E-5 0.0 0.0 0.7490201206310954 0.0 72-73 8.276008183316892E-5 0.0 0.0 0.8441114546574064 0.0 74-75 8.276008183316892E-5 0.0 0.0 0.947106376498785 0.0 76-77 8.276008183316892E-5 0.0 0.0 1.06032216844656 0.0 78-79 8.276008183316892E-5 0.0 0.0 1.1929451995842135 0.0 80-81 8.276008183316892E-5 0.0 0.0 1.382879587391336 0.0 82-83 8.276008183316892E-5 0.0 0.0 1.5738898562622898 0.0 84-85 8.276008183316892E-5 0.0 0.0 1.85072232999424 0.0 86-87 8.276008183316892E-5 0.0 0.0 2.2007147160667113 0.0 88-89 8.276008183316892E-5 0.0 0.0 2.622542853170373 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7090 0.0 30.426332 1 GTATCAA 10260 0.0 29.685928 1 GTACATG 8440 0.0 25.728456 1 TACATGG 8555 0.0 24.715078 2 ATCAACG 12450 0.0 24.312412 3 TCAACGC 12515 0.0 24.224108 4 TATCAAC 12535 0.0 24.183453 2 CAACGCA 12670 0.0 23.92677 5 AACGCAG 12960 0.0 23.429 6 GAGTACT 6130 0.0 23.251274 12-13 ACATGGG 8695 0.0 23.062204 3 TACCTGG 845 0.0 23.05415 2 TAAGGTG 535 0.0 22.203743 5 GTACTTT 6460 0.0 21.8465 14-15 CATGGGG 3160 0.0 21.804134 4 ACGCAGA 14325 0.0 21.228792 7 GTCTAGG 360 0.0 21.118225 1 GTATAGG 430 0.0 20.995445 1 CGCAGAG 14435 0.0 20.935352 8 AGTACTT 6340 0.0 20.49529 12-13 >>END_MODULE