FastQCFastQC Report
Thu 26 May 2016
SRR936719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208312
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85420.7069366190189288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75910.6282317811955852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.3887241043703944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25860.2140175716205748No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.17851349651414536No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12840.10626394507378889No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12450.1030363018822953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.029.2910791
GTATCAA120800.028.9875621
CGGACCG908.9509785E-426.3872915
GTACATG78150.025.2307531
TACATGG79650.024.2167262
GAGTACT81750.024.11268612-13
TATCAAC146350.023.8275012
TCAACGC145900.023.764844
ATCAACG146200.023.7170563
CAACGCA147800.023.4593375
AACGCAG150150.023.1535246
GTACTTT86150.022.8821114-15
ACATGGG80500.022.5399423
AGTACTT83600.022.32911712-13
GTGTAAG4950.021.1168251
ACGCAGA166950.020.7667147
CGCAGAG168500.020.5756878
ACTTTTT98400.020.29729816-17
CATGGGG28550.020.2964954
TACCTGG8450.020.2404862
Produced by FastQC (version 0.11.5)