##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936719_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1208312 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880445613384623 31.0 31.0 34.0 30.0 34.0 2 32.01029121617596 31.0 31.0 34.0 30.0 34.0 3 32.08807658949013 33.0 31.0 34.0 30.0 34.0 4 35.84076463694807 37.0 35.0 37.0 35.0 37.0 5 35.707641734916145 37.0 35.0 37.0 33.0 37.0 6 35.76780252120313 37.0 35.0 37.0 33.0 37.0 7 35.693766179596 37.0 35.0 37.0 33.0 37.0 8 35.66541340316077 37.0 35.0 37.0 33.0 37.0 9 37.2567672918915 39.0 37.0 39.0 34.0 39.0 10-11 37.158560454584574 39.0 37.0 39.0 33.0 39.0 12-13 37.02636115506591 39.0 37.0 39.0 33.0 39.0 14-15 38.08009272439568 40.0 37.0 41.0 33.0 41.0 16-17 38.05372163811995 40.0 37.0 41.0 33.0 41.0 18-19 38.235820301379114 40.0 37.5 41.0 33.5 41.0 20-21 38.265354477982505 40.0 38.0 41.0 33.5 41.0 22-23 38.164428558186955 40.0 37.5 41.0 33.0 41.0 24-25 38.03972194267706 40.0 37.0 41.0 33.0 41.0 26-27 37.81528942855819 40.0 37.0 41.0 33.0 41.0 28-29 37.8110144565311 40.0 37.0 41.0 33.0 41.0 30-31 37.632126056846246 39.5 37.0 41.0 33.0 41.0 32-33 37.37334066035925 39.0 36.0 41.0 32.0 41.0 34-35 37.15495170121625 39.0 35.5 41.0 31.5 41.0 36-37 36.928208939413 39.0 35.0 41.0 31.0 41.0 38-39 36.69658953978774 39.0 35.0 41.0 31.0 41.0 40-41 36.43058746416489 39.0 35.0 40.0 30.0 41.0 42-43 36.10871778150015 39.0 35.0 40.0 30.0 41.0 44-45 35.71310927972246 38.0 35.0 40.0 28.5 41.0 46-47 35.18698316328895 38.0 34.5 40.0 26.5 41.0 48-49 34.6754099934454 38.0 34.0 40.0 24.0 41.0 50-51 34.319531710352955 38.0 34.0 40.0 23.5 41.0 52-53 34.03686092664808 38.0 33.0 40.0 23.0 41.0 54-55 33.68984128271506 38.0 33.0 40.0 22.0 41.0 56-57 33.34830449420348 37.0 33.0 40.0 20.5 41.0 58-59 32.96660506557909 37.0 32.0 39.5 19.0 41.0 60-61 32.57775185548104 36.0 31.5 39.0 18.0 40.0 62-63 32.24831210813102 36.0 31.0 39.0 16.5 40.0 64-65 31.9300147643986 35.0 31.0 39.0 13.5 40.0 66-67 31.47931949695112 35.0 31.0 38.0 9.0 40.0 68-69 31.020658985427605 35.0 30.0 38.0 7.5 40.0 70-71 30.505501890240268 34.5 29.5 37.0 7.0 39.0 72-73 29.951733492674077 34.0 29.0 36.5 2.0 39.0 74-75 29.424237696886237 34.0 28.5 36.0 2.0 39.0 76-77 28.066301998159418 32.0 26.5 34.5 2.0 36.5 78-79 28.841342716119676 33.5 28.5 35.0 2.0 37.0 80-81 28.694775024993547 34.0 28.5 35.0 2.0 37.0 82-83 28.288043568217482 33.0 27.5 35.0 2.0 36.0 84-85 27.845889968816003 33.0 26.5 35.0 2.0 36.0 86-87 27.41964327094327 33.0 26.0 35.0 2.0 35.5 88-89 26.913036119810116 32.5 25.5 35.0 2.0 35.0 90-91 26.344741258880155 32.0 25.0 34.0 2.0 35.0 92-93 25.76408742112964 32.0 23.5 34.0 2.0 35.0 94-95 25.01072032720026 31.0 19.5 34.0 2.0 35.0 96-97 24.173977830229276 31.0 12.5 34.0 2.0 35.0 98-99 23.01838556597965 31.0 2.0 34.0 2.0 35.0 100-101 19.396025612590126 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20381080389641326 1101 2 0.39693098812118777 1101 3 0.24636993411411012 1101 4 0.16557886992941206 1101 5 0.1522626391098001 1101 6 0.21821749883269348 1101 7 0.16892932643695246 1101 8 0.09069393212684673 1101 9 0.1901991296608614 1101 10-11 0.21306371011640834 1101 12-13 0.22857646634526674 1101 14-15 0.3160369458597785 1101 16-17 0.31310285616238076 1101 18-19 0.27991459120991635 1101 20-21 0.2268927552750668 1101 22-23 0.22013588175644827 1101 24-25 0.21144413406219087 1101 26-27 0.14005131985187091 1101 28-29 0.20238226598494435 1101 30-31 0.4002060316619023 1101 32-33 0.2808829308957357 1101 34-35 0.3064456832100504 1101 36-37 0.3788981952427406 1101 38-39 0.31293294316837716 1101 40-41 0.2891735741221382 1101 42-43 0.39906049637641416 1101 44-45 0.47275793401716015 1101 46-47 0.2270807603414866 1101 48-49 0.3661926952264025 1101 50-51 0.4661859354890652 1101 52-53 0.3311028808946972 1101 54-55 0.28211165731147503 1101 56-57 0.15797163306711326 1101 58-59 0.23762671648484002 1101 60-61 0.21246529016026727 1101 62-63 0.091281401441627 1101 64-65 0.14717466800797396 1101 66-67 0.2158641085601829 1101 68-69 0.09771989171148121 1101 70-71 0.041649921732460626 1101 72-73 0.12052786125584092 1101 74-75 0.013727487410996275 1101 76-77 -0.04344284511685004 1101 78-79 0.19488138399114519 1101 80-81 0.29116022196791747 1101 82-83 0.19264319477212766 1101 84-85 0.03317304528115628 1101 86-87 0.13677774136634113 1101 88-89 0.1137486638795906 1101 90-91 0.3349464220618188 1101 92-93 0.2344644850807498 1101 94-95 0.42445543274464725 1101 96-97 0.42823197248096534 1101 98-99 -0.030758593346146057 1101 100-101 -0.1318310964142917 1102 1 0.2085821893618096 1102 2 0.34725787556030596 1102 3 0.219908929822207 1102 4 0.10711251970172242 1102 5 0.09693082596954383 1102 6 0.14373263072436515 1102 7 0.12518496688143443 1102 8 0.030516331322196777 1102 9 0.19646958165422035 1102 10-11 0.18693131553674647 1102 12-13 0.3126757519463723 1102 14-15 0.43477252182235304 1102 16-17 0.36415447613247665 1102 18-19 0.2805008529816533 1102 20-21 0.22360872488038552 1102 22-23 0.2615524169562704 1102 24-25 0.25415995210747155 1102 26-27 0.07307349584063161 1102 28-29 -0.03394598549449057 1102 30-31 0.23145426633194432 1102 32-33 0.1542379878340867 1102 34-35 0.09505801330781338 1102 36-37 0.0947100403483887 1102 38-39 0.07245883582253754 1102 40-41 0.1444420076113957 1102 42-43 0.18894730858664133 1102 44-45 0.4240010707587558 1102 46-47 0.2822844363612447 1102 48-49 0.48665123322319204 1102 50-51 0.44101525822238585 1102 52-53 0.5696018854208305 1102 54-55 0.5562224476970599 1102 56-57 0.5909256070401199 1102 58-59 0.48131871893735223 1102 60-61 0.3215602700785638 1102 62-63 0.27266431787004564 1102 64-65 0.26034454613992963 1102 66-67 0.2163525452184203 1102 68-69 0.16797072356940035 1102 70-71 -0.04167089641616428 1102 72-73 -0.1325560410017168 1102 74-75 0.16012552539745428 1102 76-77 0.2539406775517108 1102 78-79 0.17212188909940807 1102 80-81 0.3817132094228768 1102 82-83 0.41739501521482225 1102 84-85 0.07870581324963055 1102 86-87 0.2813908154860876 1102 88-89 0.09798022005669438 1102 90-91 -0.11536393155529723 1102 92-93 0.032046413613304736 1102 94-95 0.09493153792731235 1102 96-97 0.05405298007963566 1102 98-99 -0.20372992783225286 1102 100-101 -0.250806412795864 1103 1 0.1832638334391703 1103 2 0.26143885929085187 1103 3 0.19844694202071977 1103 4 0.12491810557688865 1103 5 0.2385458538015186 1103 6 0.07913323635263936 1103 7 0.1297225951906924 1103 8 0.14713615776987865 1103 9 0.10720301067338056 1103 10-11 0.28490071326034183 1103 12-13 0.2824818592233669 1103 14-15 0.370645683666055 1103 16-17 0.33190648940244216 1103 18-19 0.32005032742579687 1103 20-21 0.25605137784163645 1103 22-23 0.1915489218451185 1103 24-25 0.21257314300071073 1103 26-27 0.20256822309304567 1103 28-29 0.29445694925293253 1103 30-31 0.28745425487256426 1103 32-33 0.2986237506993916 1103 34-35 0.3385218689883658 1103 36-37 0.3194337976501558 1103 38-39 0.06834864241684357 1103 40-41 0.17073054336036364 1103 42-43 0.3811178299025215 1103 44-45 0.30566291016126 1103 46-47 0.34995265290915967 1103 48-49 0.5545920397917072 1103 50-51 0.6117210701338394 1103 52-53 0.681150649383369 1103 54-55 0.37631948000392157 1103 56-57 0.5622655524361733 1103 58-59 0.35828722569409166 1103 60-61 0.22123711554021952 1103 62-63 0.48592325117358115 1103 64-65 0.5427869126166591 1103 66-67 0.3249321836176584 1103 68-69 0.2826587849000042 1103 70-71 0.07690547605955089 1103 72-73 0.3460202168801878 1103 74-75 0.2886860266307707 1103 76-77 0.09882234512080501 1103 78-79 0.09919911673141257 1103 80-81 0.08645467146797614 1103 82-83 0.014699195883419947 1103 84-85 0.25873698276538803 1103 86-87 0.20493869707630452 1103 88-89 0.24214639253756332 1103 90-91 0.16328022315780188 1103 92-93 0.0491359314312092 1103 94-95 0.10566639936907407 1103 96-97 0.23665892921717457 1103 98-99 0.2508377813515814 1103 100-101 0.04648186798188547 1104 1 0.2992690932295865 1104 2 0.2979820769670134 1104 3 0.275655053692784 1104 4 0.09459812673497225 1104 5 0.030633622682906037 1104 6 0.11136896316313738 1104 7 0.14066118781893522 1104 8 0.15065053021531583 1104 9 0.23283934807047757 1104 10-11 0.17451882101172345 1104 12-13 0.23814976031800228 1104 14-15 0.2884167081173956 1104 16-17 0.1573509113426823 1104 18-19 0.09551574640973115 1104 20-21 0.25943227350490616 1104 22-23 0.08948734042707684 1104 24-25 0.11698688881755714 1104 26-27 0.25863311696506486 1104 28-29 0.24780696873249042 1104 30-31 0.3494899894872745 1104 32-33 0.42986379026550026 1104 34-35 0.47307714974486004 1104 36-37 0.4859169724527135 1104 38-39 0.5965568389442168 1104 40-41 0.6463001113972879 1104 42-43 0.6505537217163351 1104 44-45 0.8104114008826073 1104 46-47 0.8208336554158038 1104 48-49 0.8336076005211694 1104 50-51 0.9402833053094 1104 52-53 0.9231967028991974 1104 54-55 0.8782403378725121 1104 56-57 1.1035945785883285 1104 58-59 0.9913919142286503 1104 60-61 0.9250171408481691 1104 62-63 0.8634075142299977 1104 64-65 0.8308033299511699 1104 66-67 1.0403634003374975 1104 68-69 1.0662396541776147 1104 70-71 0.9492751937391226 1104 72-73 1.0592264592579177 1104 74-75 1.1608391155188755 1104 76-77 0.8974803615009748 1104 78-79 0.8572334280067011 1104 80-81 0.8629474900454674 1104 82-83 0.7213243515805239 1104 84-85 0.6527424816371834 1104 86-87 0.9918481242534121 1104 88-89 0.9962428290481391 1104 90-91 1.248016146622028 1104 92-93 1.1310895917609827 1104 94-95 1.2292603252154422 1104 96-97 1.3148204243553359 1104 98-99 1.046467020633024 1104 100-101 0.9429198341054139 1105 1 0.1048110679977654 1105 2 0.11254380605098291 1105 3 -0.0370313750062401 1105 4 0.07806991499585081 1105 5 0.034836852380344396 1105 6 0.10061332508246323 1105 7 0.0178563949277617 1105 8 0.06847557565960471 1105 9 0.11197325816593917 1105 10-11 0.12761506201486128 1105 12-13 0.005051045404080412 1105 14-15 0.06416032389002169 1105 16-17 0.04490492880448471 1105 18-19 0.14948742704167017 1105 20-21 0.012933496466807526 1105 22-23 -0.03759082236938838 1105 24-25 -0.04234540404510767 1105 26-27 0.18124860039867485 1105 28-29 0.13320305186832826 1105 30-31 0.06558661436120161 1105 32-33 0.12176929931238334 1105 34-35 -0.13604442880370726 1105 36-37 0.012040226419344435 1105 38-39 -0.12469232706107647 1105 40-41 0.005336260554763328 1105 42-43 0.1581330103840628 1105 44-45 -0.028455983333351753 1105 46-47 -0.161553551010293 1105 48-49 -0.3400763117683283 1105 50-51 -0.2586592014878093 1105 52-53 -0.2582283733653199 1105 54-55 -0.13659984438145756 1105 56-57 0.09922584564181136 1105 58-59 -0.07320808100320164 1105 60-61 -0.13550452953916192 1105 62-63 -0.2909643456568176 1105 64-65 -0.3089541438680463 1105 66-67 -0.07673567667955794 1105 68-69 -0.06687471271371948 1105 70-71 -0.369426362440592 1105 72-73 -0.22025998312362915 1105 74-75 -0.18281974324120398 1105 76-77 -0.09628873486164835 1105 78-79 -0.13758742595551254 1105 80-81 0.11907810700633448 1105 82-83 0.012656542169782625 1105 84-85 0.18814376482291806 1105 86-87 0.16146062206368939 1105 88-89 0.3961036888427003 1105 90-91 0.2820916762237893 1105 92-93 -0.02609616887847821 1105 94-95 0.22759855511845828 1105 96-97 0.4079937435730976 1105 98-99 0.22005608815869238 1105 100-101 -0.1763789849477071 1106 1 0.06111673470746126 1106 2 0.09526168195331763 1106 3 0.05336093566488742 1106 4 0.030612338365692437 1106 5 0.09022305646927009 1106 6 0.034801248943814755 1106 7 0.15878936153518453 1106 8 0.04195414304549416 1106 9 0.18403092813247213 1106 10-11 0.13100595278149996 1106 12-13 0.17902161465669764 1106 14-15 0.1518121196223703 1106 16-17 0.23286602540763113 1106 18-19 0.07383034886748874 1106 20-21 0.07944807779357888 1106 22-23 -0.012561282398571905 1106 24-25 0.016421761408516033 1106 26-27 0.10041830380743733 1106 28-29 0.1701776623875375 1106 30-31 0.04734878532564579 1106 32-33 0.04228195433516646 1106 34-35 0.030320218553576694 1106 36-37 0.048947813475379576 1106 38-39 0.1394372119167926 1106 40-41 0.07764713027108172 1106 42-43 0.30633926392141575 1106 44-45 0.2634038929463003 1106 46-47 0.19829790513650636 1106 48-49 0.2890009489082601 1106 50-51 0.22604455480495034 1106 52-53 0.1533726434054401 1106 54-55 0.2737649618462612 1106 56-57 0.30257503292026655 1106 58-59 0.3203189418642154 1106 60-61 0.2977525128891756 1106 62-63 0.22321980503178196 1106 64-65 0.16587978688430738 1106 66-67 -0.12395246531237802 1106 68-69 -0.037108848011751405 1106 70-71 0.0920351462327389 1106 72-73 0.2967912823786527 1106 74-75 0.20288539323842514 1106 76-77 0.21499801633732218 1106 78-79 0.02388450858986957 1106 80-81 0.035854824544639285 1106 82-83 0.17398450038685453 1106 84-85 0.36736698770820553 1106 86-87 0.3327974174485071 1106 88-89 0.14964352063863373 1106 90-91 0.2994879884439037 1106 92-93 0.4199449785632119 1106 94-95 0.10772088535050628 1106 96-97 0.14118078371766885 1106 98-99 0.4003938864131058 1106 100-101 0.44325541447241434 1107 1 0.09953225002856314 1107 2 0.13464619357666763 1107 3 0.06260782136265064 1107 4 0.04468161239667978 1107 5 0.023120480930650444 1107 6 0.05070851984689284 1107 7 -0.04704243978542877 1107 8 -0.011592525674089416 1107 9 0.009312024922486728 1107 10-11 0.029237492690675992 1107 12-13 0.07808554581946936 1107 14-15 0.14734410947050236 1107 16-17 0.16376876512334348 1107 18-19 0.1552720613969072 1107 20-21 0.0161070572011468 1107 22-23 0.11501339851461978 1107 24-25 0.2524581314044383 1107 26-27 0.1772585390777337 1107 28-29 0.14805078244685177 1107 30-31 0.33603500039094314 1107 32-33 0.11801616986236496 1107 34-35 0.07046390279801784 1107 36-37 0.2908784925844756 1107 38-39 0.2981034529391451 1107 40-41 0.3607509475977011 1107 42-43 0.5118304940028082 1107 44-45 0.34193987174553087 1107 46-47 0.36481381115223144 1107 48-49 0.19684044697152103 1107 50-51 0.21054688985174153 1107 52-53 0.2709134429194293 1107 54-55 0.5244971481713563 1107 56-57 0.4893151804118787 1107 58-59 0.43526801098343526 1107 60-61 0.27092164960384224 1107 62-63 0.1901538521979731 1107 64-65 0.3021986552840623 1107 66-67 0.29733855071957294 1107 68-69 0.23956239865470863 1107 70-71 0.26725298212426196 1107 72-73 0.24944790540143558 1107 74-75 0.27182659323742087 1107 76-77 0.09408351143335025 1107 78-79 0.44137338563361084 1107 80-81 0.30522617354982984 1107 82-83 0.35571942216006747 1107 84-85 0.39349537051362304 1107 86-87 0.41663986433989564 1107 88-89 0.37461362651696106 1107 90-91 0.49387299795395734 1107 92-93 0.4757532662429824 1107 94-95 0.5081749245505094 1107 96-97 0.22357935917595384 1107 98-99 0.4701532523313823 1107 100-101 0.1632267797112199 1108 1 -0.05212976645503886 1108 2 0.1482126219001536 1108 3 -0.08008894077597972 1108 4 0.052661005213082035 1108 5 -6.093277732617253E-4 1108 6 0.01593754886822296 1108 7 0.0386890524291843 1108 8 -0.04401544225657261 1108 9 -0.07322186802317532 1108 10-11 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-1.219202623822767 2316 44-45 -0.645337690246258 2316 46-47 -1.0412033915871817 2316 48-49 -1.1474714867908986 2316 50-51 -1.2029925386305322 2316 52-53 -1.107679249335284 2316 54-55 -0.8422442222885067 2316 56-57 -0.8701866316446711 2316 58-59 -0.8762834041349734 2316 60-61 -0.39420423126367155 2316 62-63 -0.888726708090104 2316 64-65 -0.4622460310033425 2316 66-67 -0.5565763678558611 2316 68-69 -1.214932493589803 2316 70-71 -0.5597112669581534 2316 72-73 -0.15503364802654218 2316 74-75 -0.5909771778434489 2316 76-77 -0.3288407616493032 2316 78-79 -0.7862109017220575 2316 80-81 -0.736921115877454 2316 82-83 -0.8533537660482224 2316 84-85 -0.5004326268436543 2316 86-87 -0.8728582012909349 2316 88-89 -1.1860592084293913 2316 90-91 -1.5404915494941882 2316 92-93 -1.5720545931610737 2316 94-95 -1.7919436320065536 2316 96-97 -1.1015087877686902 2316 98-99 -0.5158901079717211 2316 100-101 -0.4697689258640203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 18.0 5 48.0 6 114.0 7 254.0 8 606.0 9 1193.0 10 1954.0 11 3140.0 12 4527.0 13 6135.0 14 8094.0 15 11149.0 16 14401.0 17 18983.0 18 16201.0 19 10179.0 20 9077.0 21 9280.0 22 9690.0 23 10753.0 24 12416.0 25 14776.0 26 17900.0 27 21601.0 28 26382.0 29 32241.0 30 38822.0 31 48310.0 32 59175.0 33 74969.0 34 97931.0 35 130913.0 36 176898.0 37 211871.0 38 104522.0 39 3782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.742762856766845 19.73957458220112 15.546894205070947 20.970768355961088 2 16.087845035437805 21.244887942372493 39.09713261263081 23.570134409558896 3 19.52297675219306 25.65808368018685 30.702102143360232 24.116837424259856 4 13.016609975916776 17.512807143862087 36.769123817563376 32.70145906265776 5 13.008987326371791 38.78148440273882 33.278929896536916 14.930598374352478 6 30.944739438158358 36.84255390991731 16.75544064777971 15.457266004144623 7 28.01163613491627 34.68917202196465 19.800547047310076 17.498644795809003 8 27.603021796128075 33.67831179321486 18.951193755131225 19.767472655525836 9 27.338657017936608 16.5156983591145 20.285754247044014 35.85989037590488 10-11 25.38322055892849 27.167321023047027 25.822180032971616 21.62727838505287 12-13 26.938985957269313 24.856162977773952 27.05352591052642 21.15132515443031 14-15 23.365684463512494 25.649132999642475 26.827354705438367 24.157827831406667 16-17 21.369835236571976 29.153836444933674 27.381765739221475 22.09456257927287 18-19 21.366591891602226 26.578418021162957 31.219561852054618 20.8354282351802 20-21 22.433886780335442 25.175824666930396 31.573127999033357 20.817160553700802 22-23 22.00797891599793 25.412735727063307 31.514731945747343 21.06455341119142 24-25 21.92096048438021 25.654076323318492 31.59552994396292 20.82943324833838 26-27 21.44212044746758 25.879926608298693 31.62472244314585 21.05323050108788 28-29 21.089470720091633 26.3026545721033 31.62346795507615 20.984406752728912 30-31 21.294664840416946 25.763279966564735 31.608244607114926 21.333810585903393 32-33 21.075217201488908 25.952696733132356 31.49149505386845 21.480591011510285 34-35 21.926135893403956 25.84002317305305 31.153811139617645 21.080029793925352 36-37 22.101166059270852 25.498003187121082 31.205358269735058 21.195472483873008 38-39 21.723898054895617 25.933580166498583 31.111531373979556 21.230990404626237 40-41 21.72564773239351 25.957172467530103 31.110934556196806 21.20624524387958 42-43 21.742338345926886 26.127005934005908 30.477161939600595 21.653493780466608 44-45 21.754146675748622 26.23924380326371 30.349503458287437 21.657106062700237 46-47 22.33202186190322 26.686650467759982 29.4642443342448 21.517083336092004 48-49 22.516244151815677 26.921510807502557 28.808075273880828 21.754169766800935 50-51 22.265887993365858 27.279566363994874 28.687252983440708 21.767292659198564 52-53 22.62151172847118 27.326514380960344 28.51380328875551 21.53817060181296 54-55 21.977895564009057 27.305959276162273 28.638316590244468 22.077828569584202 56-57 22.49970720264929 27.4100118898486 28.165491131861444 21.92478977564067 58-59 22.62229062067179 26.905571103374676 28.291260424429943 22.180877851523594 60-61 22.286626104221728 27.076487231023442 28.98853351574433 21.6483531490105 62-63 22.19825392345832 27.068696831687923 29.042240023040467 21.690809221813296 64-65 21.895719378047257 27.280078195155177 28.857802838629798 21.966399588167768 66-67 22.053881597868074 27.299857857853716 28.507424702939467 22.138835841338743 68-69 22.235560659197418 27.094793250778476 28.65948708399284 22.010159006031262 70-71 22.27552755519709 27.358423026579604 28.239499072409956 22.12655034581335 72-73 22.3094866745264 27.31421037625329 28.18487778171822 22.19142516750209 74-75 22.186720320932405 27.8435926287183 27.84756538389437 22.122121666454923 76-77 22.276757375901372 27.508476834675033 27.986216919240615 22.228548870182976 78-79 22.269982292403363 27.61710539765121 27.86378682685579 22.24912548308964 80-81 22.3917018787551 27.94114908586508 27.55112977318463 22.11601926219519 82-83 22.40970845421933 27.877086718865417 27.6342181133053 22.078986713609954 84-85 22.164983008933344 27.87225995256074 27.439703645341073 22.52305339316484 86-87 22.26462346209296 27.730245353381367 27.404001330797534 22.601129853728136 88-89 22.10632730781852 27.637626590227338 27.3093910054677 22.94665509648645 90-91 22.092143124069636 27.617899902871763 27.630356485886193 22.65960048717241 92-93 22.157074276520543 27.593458611053524 27.713464354776708 22.53600275764923 94-95 21.860112132423684 27.4165389738588 27.680545492088704 23.042803401628813 96-97 21.979385248564213 27.26718609760165 27.69501522581756 23.058413428016582 98-99 21.723738680358654 27.515314930588314 27.294673995984436 23.466272393068596 100-101 21.54458339764649 27.582378429512023 27.41321241218409 23.459825760657395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 533.0 1 408.0 2 346.0 3 415.5 4 343.5 5 330.0 6 588.5 7 1210.0 8 1945.5 9 2428.0 10 2293.5 11 1972.0 12 2001.0 13 2104.5 14 2277.5 15 2508.5 16 2728.0 17 2845.0 18 2992.5 19 3262.5 20 3519.5 21 3875.5 22 4420.5 23 5018.0 24 5725.5 25 6704.5 26 8215.0 27 10009.0 28 11617.5 29 13532.5 30 15737.5 31 18145.0 32 21190.0 33 23363.5 34 25086.5 35 26834.5 36 29421.5 37 32304.5 38 33264.0 39 33273.5 40 33346.0 41 34799.5 42 36194.5 43 36859.0 44 38189.0 45 40175.5 46 40755.0 47 42158.5 48 48704.0 49 48663.0 50 42135.5 51 38870.0 52 37102.0 53 36854.5 54 36858.5 55 35131.0 56 32225.5 57 29501.5 58 27756.0 59 25316.5 60 21492.0 61 18007.0 62 15413.5 63 12175.5 64 8561.5 65 6439.5 66 4873.0 67 3864.5 68 2858.0 69 1870.5 70 1401.0 71 1077.5 72 895.0 73 826.0 74 709.5 75 560.5 76 408.5 77 258.0 78 158.0 79 108.0 80 76.5 81 59.5 82 44.5 83 25.0 84 14.5 85 9.5 86 3.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011172611047477804 2 0.01150365137481048 3 0.005710445646488656 4 1.6552016366633783E-4 5 0.0045518045008242905 6 0.0 7 5.793205728321824E-4 8 0.001986241963996054 9 8.276008183316892E-5 10-11 0.0 12-13 0.0 14-15 6.620806546653513E-4 16-17 0.005338025278239395 18-19 0.0017793417594131316 20-21 4.965604909990135E-4 22-23 2.069002045829223E-4 24-25 4.138004091658446E-4 26-27 8.276008183316892E-5 28-29 4.138004091658446E-5 30-31 5.793205728321824E-4 32-33 0.0036828236415760167 34-35 9.93120981998027E-4 36-37 9.517409410814427E-4 38-39 0.006082866014737915 40-41 4.138004091658446E-5 42-43 0.001820721800329716 44-45 0.0045518045008242905 46-47 0.0 48-49 0.0025655625368282363 50-51 0.001448301432080456 52-53 0.0010758810638311959 54-55 2.069002045829223E-4 56-57 0.011462271333893895 58-59 0.00268970265957799 60-61 0.004262144214408199 62-63 2.4828024549950677E-4 64-65 0.0043862843371579524 66-67 0.0012000211865809494 68-69 0.0020276220049126386 70-71 0.006082866014737915 72-73 0.0036000635597428477 74-75 0.006827706751236436 76-77 0.0015724415548302095 78-79 0.006082866014737915 80-81 0.0031862631505770035 82-83 0.003765583723409186 84-85 0.0024828024549950673 86-87 0.0011586411456643649 88-89 0.002772462741411159 90-91 0.00955878945173101 92-93 0.0030207429869106655 94-95 7.034606955819358E-4 96-97 2.069002045829223E-4 98-99 0.0011586411456643649 100-101 0.002606942577744821 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1208312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.967097783545746 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38271075128745 40.45770509335734 2 13.037471455498373 13.811140508671377 3 4.446219461134032 7.065100228949711 4 2.043104500911887 4.328692635272094 5 1.0601527952685519 2.807660838624437 6 0.6463552946659671 2.0541338457290883 7 0.4207508295668018 1.5600165232520946 8 0.29990941730881576 1.270826514624182 9 0.21590366176757084 1.0292211328201661 >10 1.2892969898832838 12.71693685059923 >50 0.1018511646554939 3.6833018187310427 >100 0.04939579046800418 4.805970966886928 >500 0.0053147313146992645 1.8049495507673767 >1k 0.0012505250152233564 1.2685980282962268 >5k 3.126312538058391E-4 1.3357454634188113 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8542 0.7069366190189288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7591 0.6282317811955852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 0.3887241043703944 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2586 0.2140175716205748 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 0.17851349651414536 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1284 0.10626394507378889 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1245 0.1030363018822953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.276008183316892E-5 0.0 18-19 0.0 0.0 0.0 8.276008183316892E-5 0.0 20-21 0.0 0.0 0.0 1.6552016366633783E-4 0.0 22-23 0.0 0.0 0.0 2.896602864160912E-4 0.0 24-25 0.0 0.0 0.0 7.034606955819357E-4 0.0 26-27 0.0 0.0 0.0 9.10360900164858E-4 0.0 28-29 8.276008183316892E-5 0.0 0.0 0.001531061513913625 0.0 30-31 8.276008183316892E-5 0.0 0.0 0.002731082700494574 0.0 32-33 8.276008183316892E-5 0.0 0.0 0.005172505114573057 0.0 34-35 8.276008183316892E-5 0.0 0.0 0.007324267242235449 0.0 36-37 1.6552016366633783E-4 0.0 0.0 0.010965710842894882 0.0 38-39 1.6552016366633783E-4 0.0 0.0 0.016303736121134275 0.0 40-41 1.6552016366633783E-4 0.0 0.0 0.02201418176762293 0.0 42-43 1.6552016366633783E-4 0.0 0.0 0.03144883109660419 0.0 44-45 1.6552016366633783E-4 0.0 0.0 0.039228278788922065 0.0 46-47 1.6552016366633783E-4 0.0 0.0 0.054994074378140745 0.0 48-49 1.6552016366633783E-4 0.0 0.0 0.07229093148127305 0.0 50-51 1.6552016366633783E-4 0.0 0.0 0.08300836207866842 0.0 52-53 1.6552016366633783E-4 0.0 0.0 0.09914657803613636 0.0 54-55 1.6552016366633783E-4 0.0 0.0 0.12745052602308013 0.0 56-57 2.4828024549950677E-4 0.0 0.0 0.1706512887399943 0.0 58-59 2.4828024549950677E-4 0.0 0.0 0.22936956680062764 0.0 60-61 2.4828024549950677E-4 0.0 0.0 0.2999225365634042 0.0 62-63 2.4828024549950677E-4 0.0 0.0 0.3698134256715153 0.0 64-65 2.4828024549950677E-4 0.0 0.0 0.4417319367845391 0.0 66-67 2.4828024549950677E-4 0.0 0.0 0.5428233767437549 0.0 68-69 2.4828024549950677E-4 0.0 0.0 0.6711015035851667 0.0 70-71 2.4828024549950677E-4 0.0 0.0 0.7522063837816723 0.0 72-73 2.4828024549950677E-4 0.0 0.0 0.8480839385853984 0.0 74-75 2.4828024549950677E-4 0.0 0.0 0.9511202404676937 0.0 76-77 2.4828024549950677E-4 0.0 0.0 1.064418792497302 0.0 78-79 2.4828024549950677E-4 0.0 0.0 1.1963797429802898 0.0 80-81 2.4828024549950677E-4 0.0 0.0 1.385362389846331 0.0 82-83 2.4828024549950677E-4 0.0 0.0 1.5755450578989532 0.0 84-85 2.4828024549950677E-4 0.0 0.0 1.8499774892577414 0.0 86-87 2.4828024549950677E-4 0.0 0.0 2.1976112129979675 0.0 88-89 2.4828024549950677E-4 0.0 0.0 2.616004806705553 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8370 0.0 29.291079 1 GTATCAA 12080 0.0 28.987562 1 CGGACCG 90 8.9509785E-4 26.387291 5 GTACATG 7815 0.0 25.230753 1 TACATGG 7965 0.0 24.216726 2 GAGTACT 8175 0.0 24.112686 12-13 TATCAAC 14635 0.0 23.827501 2 TCAACGC 14590 0.0 23.76484 4 ATCAACG 14620 0.0 23.717056 3 CAACGCA 14780 0.0 23.459337 5 AACGCAG 15015 0.0 23.153524 6 GTACTTT 8615 0.0 22.88211 14-15 ACATGGG 8050 0.0 22.539942 3 AGTACTT 8360 0.0 22.329117 12-13 GTGTAAG 495 0.0 21.116825 1 ACGCAGA 16695 0.0 20.766714 7 CGCAGAG 16850 0.0 20.575687 8 ACTTTTT 9840 0.0 20.297298 16-17 CATGGGG 2855 0.0 20.296495 4 TACCTGG 845 0.0 20.240486 2 >>END_MODULE