FastQCFastQC Report
Thu 26 May 2016
SRR936718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371541
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81880.5969927257005077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65300.4761068024944205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.29499664975381706No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21770.15872657106130988No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.12934356318914272No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14590.10637669599377635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79700.031.7245921
GTATCAA117600.030.3915791
GTACATG94900.025.992251
TACATGG97850.024.9162922
TATCAAC142750.024.903042
ATCAACG143400.024.7238753
TCAACGC144550.024.5600594
CAACGCA145600.024.3485245
GAGTACT73650.024.03131112-13
AACGCAG148450.023.9130846
ACATGGG99650.023.416993
GTACTTT77800.022.7465314-15
AGTACTT75300.021.80102712-13
ACGCAGA162700.021.786287
CGCAGAG163800.021.6399778
GCAGAGT166750.020.972199
GACCGGT1604.977968E-520.7878677
CATGGGA60150.020.780074
TACCTGG9800.019.8831272
ACTTTTT90200.019.8301916-17
Produced by FastQC (version 0.11.5)