FastQCFastQC Report
Thu 26 May 2016
SRR936718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371541
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92270.6727469321004622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.607491864989818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53880.39284279507502873No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27140.19787961132769635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22060.16084098105707376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13960.10178332255470306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90050.032.281721
GTATCAA129450.029.2117561
TAAGGTG7250.026.8575655
GTACATG93350.024.140351
GTAAGGT7100.024.0822224
TATCAAC161600.023.2237952
ATCAACG162400.023.1636733
TCAACGC162900.023.1500454
TACATGG96300.022.9389822
CAACGCA164700.022.8665355
GAGTACT92350.022.78177812-13
AACGCAG168000.022.445646
GTACTTT95000.022.07129714-15
ACATGGG95500.021.8835413
AGTACTT94000.021.72507912-13
AGGTAAG6200.021.4542732
GGTAAGG6750.021.1098793
ACGCAGA186950.020.1450587
CGCAGAG189200.019.8803868
AGAGTAC154450.019.77169210-11
Produced by FastQC (version 0.11.5)