##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936718_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1371541 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.417959069397124 31.0 31.0 34.0 30.0 34.0 2 31.657900857502618 31.0 31.0 34.0 30.0 34.0 3 31.89988851955574 31.0 31.0 34.0 30.0 34.0 4 35.7317805300753 37.0 35.0 37.0 35.0 37.0 5 35.545986594640624 37.0 35.0 37.0 33.0 37.0 6 35.659065970320974 37.0 35.0 37.0 33.0 37.0 7 35.59562419205842 37.0 35.0 37.0 33.0 37.0 8 35.56892502666709 37.0 35.0 37.0 33.0 37.0 9 37.17634471007429 39.0 37.0 39.0 33.0 39.0 10-11 37.07096251588541 39.0 37.0 39.0 33.0 39.0 12-13 36.94119169605575 39.0 37.0 39.0 33.0 39.0 14-15 38.000625573715986 40.0 37.0 41.0 33.0 41.0 16-17 37.926602631638424 40.0 37.0 41.0 33.0 41.0 18-19 38.02720042638171 40.0 37.0 41.0 33.0 41.0 20-21 38.11747953579222 40.0 37.0 41.0 33.0 41.0 22-23 38.02890580740933 40.0 37.0 41.0 33.0 41.0 24-25 37.90871290030702 40.0 37.0 41.0 33.0 41.0 26-27 37.68333575153787 39.5 37.0 41.0 32.0 41.0 28-29 37.575361946890396 39.0 36.5 41.0 32.0 41.0 30-31 37.50052823794549 39.0 36.5 41.0 32.0 41.0 32-33 37.26918043281243 39.0 36.0 41.0 32.0 41.0 34-35 37.074071427686086 39.0 35.0 41.0 31.0 41.0 36-37 36.81542549584737 39.0 35.0 41.0 31.0 41.0 38-39 36.6174303210768 39.0 35.0 41.0 30.0 41.0 40-41 36.34263722338596 39.0 35.0 40.0 30.0 41.0 42-43 36.036508205004445 39.0 35.0 40.0 30.0 41.0 44-45 35.667966178189346 38.0 35.0 40.0 28.5 41.0 46-47 35.14799120113799 38.0 34.5 40.0 26.5 41.0 48-49 34.570490783724296 38.0 34.0 40.0 24.0 41.0 50-51 34.34721054638541 38.0 34.0 40.0 23.5 41.0 52-53 34.05877585868742 38.0 33.0 40.0 23.0 41.0 54-55 33.76774044669463 38.0 33.0 40.0 22.0 41.0 56-57 33.47964807468388 37.0 33.0 40.0 21.0 41.0 58-59 33.10254268738594 37.0 32.5 40.0 19.0 41.0 60-61 32.71450361308922 36.0 32.0 39.0 18.0 41.0 62-63 32.41647242043803 36.0 31.0 39.0 18.0 40.0 64-65 31.98205230467044 35.0 31.0 39.0 14.0 40.0 66-67 31.575303253785343 35.0 31.0 38.0 12.0 40.0 68-69 31.113587927739673 35.0 30.0 38.0 8.0 40.0 70-71 30.63095197299971 34.5 29.5 37.0 7.0 39.0 72-73 30.217589193469244 34.0 29.0 36.5 6.5 39.0 74-75 29.844476395528822 34.0 29.0 36.0 2.0 39.0 76-77 28.451473196936874 32.5 27.0 35.0 2.0 37.0 78-79 29.24814533433561 34.0 29.0 35.0 2.0 37.0 80-81 29.095489671836276 34.0 29.0 35.0 2.0 37.0 82-83 28.756693383573662 34.0 29.0 35.0 2.0 36.5 84-85 28.316814444482517 33.5 28.0 35.0 2.0 36.0 86-87 27.924793717431708 33.0 27.0 35.0 2.0 36.0 88-89 27.49936749976851 33.0 26.0 35.0 2.0 35.0 90-91 27.043692459795224 33.0 25.5 35.0 2.0 35.0 92-93 26.574137047306643 32.5 25.0 34.5 2.0 35.0 94-95 26.038890197230707 32.0 24.5 34.0 2.0 35.0 96-97 25.359214926859643 32.0 21.5 34.0 2.0 35.0 98-99 24.484715732158207 32.0 10.0 34.0 2.0 35.0 100-101 21.768025162937164 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3276797989348026 1101 2 0.2912116495013706 1101 3 0.07801367595517661 1101 4 0.08774217512817728 1101 5 0.20829361592723927 1101 6 0.11801388247027944 1101 7 0.07211025690981643 1101 8 0.09025198658176947 1101 9 0.13367255750472395 1101 10-11 0.13087491391247852 1101 12-13 0.1679566730996811 1101 14-15 0.2735290735627416 1101 16-17 0.20683037453611774 1101 18-19 0.21785974947726316 1101 20-21 0.08352660744422025 1101 22-23 -0.08367987333060256 1101 24-25 -0.06006178469600343 1101 26-27 0.06155909552432348 1101 28-29 -0.03874415981008639 1101 30-31 0.019067869918096392 1101 32-33 -0.021234879661015782 1101 34-35 0.26922772584021004 1101 36-37 0.25965787010220254 1101 38-39 0.2390648932554953 1101 40-41 0.32262370546609276 1101 42-43 0.45341823329786024 1101 44-45 0.5374850145285208 1101 46-47 0.768879964024265 1101 48-49 0.679324206413547 1101 50-51 0.5262916428395528 1101 52-53 0.3984413243734579 1101 54-55 0.3733851306130944 1101 56-57 0.46295885462492237 1101 58-59 0.33421933199218046 1101 60-61 0.38825584143279457 1101 62-63 0.18940482235233702 1101 64-65 0.32465342683115495 1101 66-67 0.13051177833372307 1101 68-69 -0.058356910382080684 1101 70-71 -0.11587708917794615 1101 72-73 0.043709358407618026 1101 74-75 -0.004265317636274801 1101 76-77 0.26561408067758663 1101 78-79 0.2460137537631546 1101 80-81 0.11441892029960954 1101 82-83 0.21740453579858254 1101 84-85 0.29785394028929346 1101 86-87 0.14827893811057535 1101 88-89 0.07129035156566133 1101 90-91 0.036779632965359355 1101 92-93 0.28734393013583315 1101 94-95 0.02822737404093445 1101 96-97 -0.048876785130346434 1101 98-99 -0.13016845066360716 1101 100-101 -0.07862590058993035 1102 1 0.45697803443633234 1102 2 0.5184525584058051 1102 3 0.1865262555170304 1102 4 0.06468254443745991 1102 5 0.10110055448422628 1102 6 0.1657785169959567 1102 7 0.21461977161690982 1102 8 3.445605664396112E-4 1102 9 0.10678367588590731 1102 10-11 0.22268619010615964 1102 12-13 0.1939621111137484 1102 14-15 0.12784863893705278 1102 16-17 0.23029284512477943 1102 18-19 0.18353436285222102 1102 20-21 0.22578452572133045 1102 22-23 -0.058519581219101724 1102 24-25 -0.011001000140957728 1102 26-27 -0.21283095839364563 1102 28-29 -0.17740715716741562 1102 30-31 -0.12063513724343977 1102 32-33 0.008397156016350493 1102 34-35 0.0736244838034068 1102 36-37 0.06874784919035903 1102 38-39 -0.04114511316812752 1102 40-41 0.27065619022620524 1102 42-43 0.18703226931484096 1102 44-45 -0.008609191068089217 1102 46-47 0.22466844750276493 1102 48-49 -0.030861891858101842 1102 50-51 -0.005451180430711133 1102 52-53 -0.17148223081651537 1102 54-55 0.2000903650132031 1102 56-57 0.12468018444577211 1102 58-59 -0.05553716212970272 1102 60-61 0.0031706283963188753 1102 62-63 -0.04125055389207688 1102 64-65 -0.2015036441190361 1102 66-67 0.11143199292548545 1102 68-69 0.16688070414103606 1102 70-71 -0.17812357786285915 1102 72-73 -0.09663497298720003 1102 74-75 -0.4113935196487333 1102 76-77 -0.04118011344268524 1102 78-79 0.2821267992327847 1102 80-81 0.36827569364420043 1102 82-83 0.35034351963980725 1102 84-85 0.2644521124892627 1102 86-87 0.1421298850115278 1102 88-89 0.044496500177363174 1102 90-91 0.24495023352006484 1102 92-93 0.3776587398442892 1102 94-95 -0.049802960854943734 1102 96-97 -0.1884612641491792 1102 98-99 -0.4601102952782732 1102 100-101 -0.7618765108865304 1103 1 0.36176539532142726 1103 2 0.41575660521812097 1103 3 0.26116548048289445 1103 4 0.1605576417229031 1103 5 0.01413929308839812 1103 6 0.058299657744733224 1103 7 0.06432929104648366 1103 8 0.1431205433131808 1103 9 0.026933357890257525 1103 10-11 0.0776137468461684 1103 12-13 0.03860348487589249 1103 14-15 -0.0024864684634522405 1103 16-17 0.010995213255512226 1103 18-19 0.173265737112267 1103 20-21 0.1624369631638487 1103 22-23 0.13691697819118076 1103 24-25 0.1718820031425068 1103 26-27 0.07448498541270965 1103 28-29 0.16304380179843037 1103 30-31 0.3495298745880362 1103 32-33 0.2175748745712056 1103 34-35 0.3150251059061375 1103 36-37 0.22743252381805235 1103 38-39 0.32549338330338884 1103 40-41 0.4993410482877252 1103 42-43 0.592653861048781 1103 44-45 0.6594063693615695 1103 46-47 0.5419525000277332 1103 48-49 0.6803873187213227 1103 50-51 0.5082815931674034 1103 52-53 0.625474601962587 1103 54-55 0.5476381167695834 1103 56-57 0.6754783836496685 1103 58-59 0.5968721101882934 1103 60-61 0.5375260812289255 1103 62-63 0.41597280824036176 1103 64-65 0.3481685334239444 1103 66-67 0.3383155307875363 1103 68-69 0.26400380658358813 1103 70-71 0.31430440470568755 1103 72-73 0.19774513859297826 1103 74-75 0.233966259601754 1103 76-77 0.36651703206488406 1103 78-79 0.4868200847685067 1103 80-81 0.556427047516884 1103 82-83 0.48417736527762045 1103 84-85 0.5163017806050583 1103 86-87 0.44793962744422444 1103 88-89 0.1845285748925818 1103 90-91 0.16930041918130456 1103 92-93 0.2673222435876461 1103 94-95 0.21757477394690383 1103 96-97 -0.005722780518027548 1103 98-99 -0.09737985653076109 1103 100-101 -0.16689250893688978 1104 1 0.3798996625063218 1104 2 0.4299062965675411 1104 3 0.20832823720378713 1104 4 0.08507598510294656 1104 5 0.10676346430442862 1104 6 0.12451677823277407 1104 7 0.13438600245400778 1104 8 0.11768907377953042 1104 9 0.06482098342767983 1104 10-11 0.09069765965447374 1104 12-13 0.09620919839684916 1104 14-15 0.21811881567909808 1104 16-17 0.11191210344058788 1104 18-19 0.1786934704961496 1104 20-21 0.11801372431235535 1104 22-23 0.11636062645777656 1104 24-25 0.2699891135373633 1104 26-27 0.2966236244504543 1104 28-29 0.22657075505195223 1104 30-31 0.22726813778027122 1104 32-33 0.22411676127106972 1104 34-35 0.29251745237547766 1104 36-37 0.42978267354286004 1104 38-39 0.4682558478239045 1104 40-41 0.4173788447400639 1104 42-43 0.32719149857534546 1104 44-45 0.5117350501661164 1104 46-47 0.6490771145630276 1104 48-49 0.5592971715616315 1104 50-51 0.5576089468023397 1104 52-53 0.5260921819412872 1104 54-55 0.6233768427982795 1104 56-57 0.6331717851630927 1104 58-59 0.6590204520627267 1104 60-61 0.5588494589313626 1104 62-63 0.4247866364555435 1104 64-65 0.42682229271552785 1104 66-67 0.5089227666501124 1104 68-69 0.653697191367975 1104 70-71 0.5578291553037502 1104 72-73 0.4895803966940093 1104 74-75 0.5551282021765758 1104 76-77 0.6952806453120459 1104 78-79 0.771828086820598 1104 80-81 0.7507918741762616 1104 82-83 0.7132440527626329 1104 84-85 0.7725871555304735 1104 86-87 0.7566473657353008 1104 88-89 0.8832345845173499 1104 90-91 0.8603997394251017 1104 92-93 0.7279736162044586 1104 94-95 0.598527520016976 1104 96-97 0.31180380469685787 1104 98-99 0.34696712583126654 1104 100-101 0.2199174883307009 1105 1 0.35490589059408606 1105 2 0.35155118011748954 1105 3 0.22003005459017544 1105 4 0.19908492924459154 1105 5 0.13900733799273723 1105 6 0.10520150331586109 1105 7 0.1334283959840903 1105 8 0.07461622641932308 1105 9 0.12775353171814885 1105 10-11 0.12040032163032777 1105 12-13 0.07750017465720305 1105 14-15 0.14756141701464287 1105 16-17 0.08522778546176824 1105 18-19 -0.0021182468154918865 1105 20-21 0.1082248412971083 1105 22-23 0.08824156972445962 1105 24-25 0.03926155136282006 1105 26-27 -0.0011215039696779172 1105 28-29 0.15026954042293283 1105 30-31 0.30684411133672285 1105 32-33 0.19505285805251305 1105 34-35 0.13774658524337724 1105 36-37 0.2074718403158613 1105 38-39 0.29933279053157946 1105 40-41 0.2501154968846535 1105 42-43 0.1925955343762169 1105 44-45 0.020254770418645762 1105 46-47 0.17714148564768095 1105 48-49 0.19330055599566265 1105 50-51 0.08442238367383936 1105 52-53 -0.020226866285391054 1105 54-55 0.09872364095354413 1105 56-57 0.10212431194386795 1105 58-59 0.0695140204228224 1105 60-61 0.11984157812176477 1105 62-63 0.023488308013540404 1105 64-65 0.1005689280290909 1105 66-67 -0.051475861347721974 1105 68-69 -0.043464167752677696 1105 70-71 -0.056893740362109924 1105 72-73 0.09060408165606049 1105 74-75 0.0832278151237098 1105 76-77 -0.07784652312582807 1105 78-79 0.057764760839443596 1105 80-81 -0.0021502137218050166 1105 82-83 0.14461963987383442 1105 84-85 0.29190394949317877 1105 86-87 0.15837938175211974 1105 88-89 0.03858587904817412 1105 90-91 -0.18049327721756114 1105 92-93 -0.09630711561104377 1105 94-95 0.2080581652468716 1105 96-97 -0.2065379137692709 1105 98-99 -0.10163626346371402 1105 100-101 0.025177943922916057 1106 1 0.3177677437432145 1106 2 0.2936837397603078 1106 3 0.11789488925391467 1106 4 -0.06520754982216914 1106 5 -0.19448333913679505 1106 6 -0.1418162304323758 1106 7 -0.051533746296797744 1106 8 -0.026583895847828387 1106 9 -0.08827849826737122 1106 10-11 -0.0916981134503203 1106 12-13 0.05136957930636754 1106 14-15 -0.11404018248025238 1106 16-17 0.013355745053480916 1106 18-19 -0.1277412330404033 1106 20-21 -0.13354542673855008 1106 22-23 0.0023338613817287523 1106 24-25 -0.13813205366415815 1106 26-27 -0.1734079498539387 1106 28-29 -0.05230708951347651 1106 30-31 -0.12990518587768918 1106 32-33 -0.07431571227365197 1106 34-35 -0.3722382315813917 1106 36-37 -0.01894788678786341 1106 38-39 0.09517620839962149 1106 40-41 0.09266797404725224 1106 42-43 -0.08903182139614785 1106 44-45 0.012479782069100054 1106 46-47 0.07210197431543008 1106 48-49 -0.06196720406006051 1106 50-51 -0.13048468827837922 1106 52-53 0.0947177877837575 1106 54-55 -0.11751433903271646 1106 56-57 -0.26750432663141765 1106 58-59 -0.2956799283552556 1106 60-61 0.1913949221785316 1106 62-63 -0.20362593661096895 1106 64-65 -0.06715905761955554 1106 66-67 0.037024511967974405 1106 68-69 -0.08874861426831515 1106 70-71 0.006665135782267129 1106 72-73 0.21741516387070448 1106 74-75 0.18287704869906918 1106 76-77 0.2784108993422656 1106 78-79 0.19305336644269033 1106 80-81 0.07128173508634106 1106 82-83 0.22564417849071106 1106 84-85 0.38590734691655015 1106 86-87 0.1417610707425645 1106 88-89 0.11548390122663577 1106 90-91 0.004689847910842815 1106 92-93 0.35027165901150425 1106 94-95 0.20096566814069305 1106 96-97 0.1128299049145518 1106 98-99 -0.12475828508094722 1106 100-101 -0.030791348982081246 1107 1 0.5502165915615898 1107 2 0.511266170408085 1107 3 0.31666999891146475 1107 4 0.14975599521699934 1107 5 -0.0632756610964691 1107 6 0.2013469553617142 1107 7 0.11951602121823157 1107 8 0.16500219675837968 1107 9 0.26235768012597305 1107 10-11 0.2705405924625808 1107 12-13 0.3063302342884242 1107 14-15 0.2438638950610752 1107 16-17 0.2762238157757366 1107 18-19 0.3242376329597292 1107 20-21 0.28752469007097403 1107 22-23 0.2868730161680588 1107 24-25 0.22447339683720458 1107 26-27 0.26760561878126055 1107 28-29 0.4386854896246959 1107 30-31 0.35716546687280726 1107 32-33 0.2778276857750157 1107 34-35 0.3265309333384394 1107 36-37 0.3151495739860124 1107 38-39 0.1479839994814185 1107 40-41 0.13181498649644396 1107 42-43 0.27330840234548504 1107 44-45 0.16066608832003482 1107 46-47 0.3515993055854807 1107 48-49 0.49696435280272766 1107 50-51 0.42956059233153354 1107 52-53 0.1555578340978343 1107 54-55 0.32633486949404755 1107 56-57 0.45443673998256173 1107 58-59 0.2473503696864512 1107 60-61 0.17927817484634545 1107 62-63 0.3192254065339313 1107 64-65 0.4730474900060244 1107 66-67 0.5351427738364123 1107 68-69 0.34944646275719293 1107 70-71 0.48949444681460363 1107 72-73 0.49437957958529566 1107 74-75 0.6999786872516971 1107 76-77 0.6574793863790802 1107 78-79 0.6732768245606415 1107 80-81 0.8273375936710927 1107 82-83 0.6595231472890681 1107 84-85 0.7340805747445067 1107 86-87 0.7307425908394372 1107 88-89 0.8593850576153095 1107 90-91 0.7080552096618504 1107 92-93 0.847739429387321 1107 94-95 0.7364745011063683 1107 96-97 0.7901808657670806 1107 98-99 1.0688748550941405 1107 100-101 1.3520929737385252 1108 1 0.4313221287311322 1108 2 0.46800253677955794 1108 3 0.1848613602467637 1108 4 0.14594725626495375 1108 5 -0.06029865306039994 1108 6 0.00792318900506217 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-1.0619145632747333 2316 58-59 -1.2370198638491168 2316 60-61 -1.2673738605398839 2316 62-63 -1.3293878700820088 2316 64-65 -1.2021346948565323 2316 66-67 -1.3393691402062267 2316 68-69 -1.332015168263382 2316 70-71 -1.6236254054038177 2316 72-73 -1.7371765333523683 2316 74-75 -1.5955285493005142 2316 76-77 -1.6942323039574987 2316 78-79 -1.4718698554380047 2316 80-81 -1.0221836414575378 2316 82-83 -1.2718651861007544 2316 84-85 -1.2865444464397804 2316 86-87 -1.3745716992327601 2316 88-89 -1.2972947715546077 2316 90-91 -1.4281927990152319 2316 92-93 -1.5683237675756985 2316 94-95 -1.5215594276466398 2316 96-97 -1.5841289501838354 2316 98-99 -2.1301256702761613 2316 100-101 -2.9420334710298057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 32.0 5 76.0 6 138.0 7 330.0 8 757.0 9 1457.0 10 2366.0 11 3794.0 12 5317.0 13 7004.0 14 9324.0 15 12555.0 16 16208.0 17 20833.0 18 17460.0 19 10923.0 20 9524.0 21 9550.0 22 10035.0 23 11415.0 24 13232.0 25 15608.0 26 19126.0 27 23730.0 28 28765.0 29 35377.0 30 42937.0 31 52642.0 32 65299.0 33 81733.0 34 106371.0 35 142499.0 36 194718.0 37 244162.0 38 147517.0 39 8714.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56336562620066 19.778662224277248 15.578082147331877 21.079890002190222 2 15.942072314810426 21.288424307168405 39.19729612466411 23.572207253357057 3 19.503233756480274 25.734649682457512 30.600742269243952 24.16137429181827 4 13.042552508845054 17.577447082809535 36.70332539004279 32.676675018302625 5 13.071282593812361 38.87649002107848 33.165541533209726 14.886685851899434 6 31.032903865068562 36.817419238652 16.748897772651347 15.400779123628094 7 28.0028814304494 34.63782708646697 19.7884715075962 17.570819975487424 8 27.691406964866527 33.66906275495957 18.917990785547058 19.721539494626846 9 27.311292001948257 16.549299882463085 20.260462389645085 35.87894572594358 10-11 25.371580892552416 27.122133283170687 25.852063212224813 21.654222612052084 12-13 26.91559654767004 24.77985472508043 27.076328572860746 21.22822015438878 14-15 23.46099149095488 25.574782516447748 26.769169412058925 24.195056580538456 16-17 21.409677144175784 29.068252425556363 27.368667797754497 22.153402632513355 18-19 21.34449498775465 26.524325557894368 31.208582171440735 20.922597282910242 20-21 22.49229151735165 25.202454757094394 31.42308541997651 20.882168305577448 22-23 22.048927378150925 25.372788269970055 31.45548104387642 21.122803308002602 24-25 21.982281244235498 25.672655793738574 31.471352296431533 20.873710665594395 26-27 21.539575389862716 25.823116415446716 31.5650540396 21.072254155090572 28-29 21.143115663330516 26.242452832252187 31.540945549567965 21.07348595484933 30-31 21.39889416316908 25.75286694049501 31.508876332853458 21.33936256348245 32-33 21.17844182490709 25.93032098689818 31.322599014647583 21.568638173547143 34-35 21.944045761982135 25.82321295573598 31.060970892757478 21.17177038952441 36-37 22.17525431934577 25.548047982360973 31.044094658127136 21.232603040166122 38-39 21.823943094602814 25.82955139097343 31.022912573790258 21.3235929406335 40-41 21.74127185795089 25.896146365560273 31.05434023758702 21.30824153890182 42-43 21.792636570392716 26.026415361247896 30.485693806106028 21.695254262253364 44-45 21.773690807159685 26.201064080325885 30.297926450569552 21.727318661944874 46-47 22.413000499811705 26.608247148320856 29.43737825975845 21.54137409210899 48-49 22.544126247077816 26.858931636583183 28.79686978181516 21.80007233452384 50-51 22.325010316885134 27.14277694092326 28.694246896549714 21.8379658456419 52-53 22.683918167775964 27.17596290521384 28.440842908217817 21.699276018792375 54-55 22.02598232163769 27.223029997214514 28.627039348283574 22.123948332864224 56-57 22.480755243888435 27.38699687016375 28.180010244426175 21.952237641521645 58-59 22.725882427139904 26.87589021050072 28.245798169980578 22.152429192378793 60-61 22.36549245273182 27.027907921250144 28.878604607583252 21.727995018434783 62-63 22.291173818906177 27.024809085861506 28.964482879430342 21.719534215801968 64-65 22.0093518259907 27.193803175566238 28.71238103294506 22.084463965498006 66-67 22.110498720006184 27.260696570374872 28.362563079159838 22.266241630459113 68-69 22.280615732344174 27.075964018482907 28.616898918184887 22.026521330988032 70-71 22.288867614859704 27.32072640520998 28.191037541532353 22.199368438397954 72-73 22.394919291512245 27.270201911572457 28.06620436428805 22.268674432627243 74-75 22.234005399765667 27.818490296680885 27.897634850143017 22.049869453410434 76-77 22.344057538933892 27.448600042215826 28.027955773269653 22.179386645580625 78-79 22.324470319208512 27.57271443905171 27.827783740899758 22.275031500840022 80-81 22.421204195831223 27.9051070378874 27.644444541674822 22.029244224606554 82-83 22.50620820558704 27.807255588478824 27.57752190235972 22.10901430357442 84-85 22.162292427663456 27.81749129278095 27.51433824588319 22.50587803367241 86-87 22.330773886348886 27.688914167377625 27.40504160012834 22.575270346145153 88-89 22.21146889622741 27.574915107880088 27.34361325367282 22.87000274221968 90-91 22.243590164328957 27.55990498923216 27.636297205466988 22.5602076409719 92-93 22.253655861920123 27.546781033265866 27.674196643665024 22.525366461148995 94-95 21.941358848381174 27.334066876375395 27.700911314208714 23.02366296103472 96-97 22.038951976146674 27.249028534010606 27.722091818393285 22.989927671449436 98-99 21.809855172647634 27.46046624906163 27.374750390388158 23.35492818790258 100-101 21.623033709503822 27.454782245997418 27.567844861028874 23.35433918346989 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 628.0 1 474.5 2 392.0 3 487.0 4 413.5 5 369.5 6 658.0 7 1442.0 8 2303.0 9 2703.5 10 2574.0 11 2258.0 12 2206.0 13 2330.0 14 2510.5 15 2739.5 16 2974.5 17 3181.5 18 3386.5 19 3656.5 20 3935.5 21 4303.0 22 4860.5 23 5548.0 24 6336.0 25 7521.0 26 9419.0 27 11478.0 28 13203.0 29 15169.0 30 17554.5 31 20375.0 32 23873.0 33 26420.0 34 28436.5 35 30416.5 36 33356.0 37 36571.5 38 37603.0 39 37596.5 40 37709.0 41 39432.5 42 40818.0 43 41590.0 44 43404.0 45 45501.0 46 46081.5 47 47970.5 48 55066.5 49 54942.5 50 47768.0 51 44268.0 52 42439.0 53 41999.0 54 42179.5 55 40023.0 56 36540.0 57 33690.5 58 31892.5 59 28956.0 60 24412.0 61 20680.5 62 17733.0 63 14035.5 64 10015.5 65 7535.5 66 5788.5 67 4480.0 68 3294.5 69 2199.0 70 1609.5 71 1242.5 72 1057.5 73 978.0 74 786.5 75 634.5 76 494.5 77 317.0 78 203.5 79 135.5 80 89.5 81 71.0 82 55.5 83 36.0 84 16.5 85 8.5 86 5.0 87 2.0 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1311364370441714 2 0.012832281353601533 3 0.005176658955146073 4 0.011155335494892242 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0041559093020186785 10-11 1.8227672377274905E-4 12-13 2.5518741328184867E-4 14-15 3.645534475454981E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001348847755918343 24-25 0.0 26-27 3.645534475454981E-5 28-29 0.0 30-31 3.645534475454981E-5 32-33 0.0037913558544731803 34-35 6.926515503364464E-4 36-37 0.002697695511836686 38-39 1.0936603426364944E-4 40-41 0.0031716149936458337 42-43 0.010353317910292146 44-45 0.0020414993062547893 46-47 0.0017863118929729404 48-49 0.009842943083728448 50-51 2.1873206852729887E-4 52-53 0.0020050439615002394 54-55 0.010717871357837644 56-57 0.004629828783827826 58-59 0.0018592225824820403 60-61 0.006160953263518918 62-63 0.0034632577516822316 64-65 0.018810957893347705 66-67 0.0036455344754549807 68-69 2.5518741328184867E-4 70-71 0.0014582137901819924 72-73 0.0 74-75 0.0 76-77 0.0012759370664092433 78-79 0.010863692736855843 80-81 0.016587181863320165 82-83 0.012431272561301486 84-85 0.008858648775355603 86-87 0.011009514115874043 88-89 0.028362258219039753 90-91 0.023914706158984674 92-93 0.003280981027909483 94-95 0.008384729293546457 96-97 0.01166571032145594 98-99 0.010900148081610392 100-101 0.01290519204311063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1371541.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.12634436754499 #Duplication Level Percentage of deduplicated Percentage of total 1 75.68293725461918 39.4507485008158 2 13.356626376957555 13.924642122278485 3 4.555176583482757 7.123341097367976 4 2.076935003562776 4.330521168988861 5 1.1456267768435995 2.985866794321504 6 0.7098504061817686 2.2201144033243536 7 0.44066883771350995 1.607931891069015 8 0.33115042176466924 1.380932873789033 9 0.2310691941216283 1.084031314696359 >10 1.3041780919711188 12.74188231684478 >50 0.10746203137853852 3.847379196558847 >100 0.05144974432512653 4.896224407380907 >500 0.005187005140452326 1.757979034221984 >1k 0.0012617039530829982 0.9715397289762346 >5k 4.2056798436099945E-4 1.6768651493657507 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9227 0.6727469321004622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8332 0.607491864989818 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5388 0.39284279507502873 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2714 0.19787961132769635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 0.16084098105707376 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1396 0.10178332255470306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.291068950909961E-5 0.0 0.0 0.0 0.0 3 7.291068950909961E-5 0.0 0.0 0.0 0.0 4 7.291068950909961E-5 0.0 0.0 0.0 0.0 5 7.291068950909961E-5 0.0 0.0 0.0 0.0 6 7.291068950909961E-5 0.0 0.0 0.0 0.0 7 7.291068950909961E-5 0.0 0.0 0.0 0.0 8 7.291068950909961E-5 0.0 0.0 0.0 0.0 9 7.291068950909961E-5 0.0 0.0 0.0 0.0 10-11 7.291068950909961E-5 0.0 0.0 0.0 0.0 12-13 7.291068950909961E-5 0.0 0.0 0.0 0.0 14-15 7.291068950909961E-5 0.0 0.0 0.0 0.0 16-17 7.291068950909961E-5 0.0 0.0 0.0 0.0 18-19 7.291068950909961E-5 0.0 0.0 7.291068950909961E-5 0.0 20-21 7.291068950909961E-5 0.0 0.0 1.0936603426364942E-4 0.0 22-23 7.291068950909961E-5 0.0 0.0 1.4582137901819923E-4 0.0 24-25 7.291068950909961E-5 0.0 0.0 4.010087923000479E-4 0.0 26-27 7.291068950909961E-5 0.0 0.0 6.926515503364464E-4 0.0 28-29 2.1873206852729884E-4 0.0 0.0 0.001312392411163793 0.0 30-31 2.1873206852729884E-4 0.0 0.0 0.0027341508565912357 0.0 32-33 2.1873206852729884E-4 0.0 0.0 0.004374641370545977 0.0 34-35 2.1873206852729884E-4 0.0 0.0 0.006634872745328065 0.0 36-37 2.1873206852729884E-4 0.0 0.0 0.009259657567655653 0.0 38-39 2.1873206852729884E-4 0.0 0.0 0.013780120317219828 0.0 40-41 2.1873206852729884E-4 0.0 0.0 0.019321332719911398 0.0 42-43 2.5518741328184867E-4 0.0 0.0 0.02683113373934866 0.0 44-45 2.9164275803639846E-4 0.0 0.0 0.03295563165811303 0.0 46-47 2.9164275803639846E-4 0.0 0.0 0.04607955576975096 0.0 48-49 2.9164275803639846E-4 0.0 0.0 0.06284901435684387 0.0 50-51 2.9164275803639846E-4 0.0 0.0 0.07178057382170858 0.0 52-53 2.9164275803639846E-4 0.0 0.0 0.0861439796550012 0.0 54-55 2.9164275803639846E-4 0.0 0.0 0.10998577512447677 0.0 56-57 2.9164275803639846E-4 0.0 0.0 0.14480062936507182 0.0 58-59 2.9164275803639846E-4 0.0 0.0 0.1921196668564775 0.0 60-61 2.9164275803639846E-4 0.0 0.0 0.24837026381274785 0.0 62-63 2.9164275803639846E-4 3.645534475454981E-5 0.0 0.2999181212956813 3.645534475454981E-5 64-65 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.3604704489329885 7.291068950909961E-5 66-67 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.446833160656517 7.291068950909961E-5 68-69 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.5544857937167026 7.291068950909961E-5 70-71 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.6200689589301377 7.291068950909961E-5 72-73 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.7044630820369204 7.291068950909961E-5 74-75 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.790497695657658 7.291068950909961E-5 76-77 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.8803236651328687 7.291068950909961E-5 78-79 2.9164275803639846E-4 7.291068950909961E-5 0.0 0.9881221195720726 7.291068950909961E-5 80-81 2.9164275803639846E-4 7.291068950909961E-5 0.0 1.1458279409802552 7.291068950909961E-5 82-83 2.9164275803639846E-4 7.291068950909961E-5 0.0 1.320011578217494 7.291068950909961E-5 84-85 2.9164275803639846E-4 7.291068950909961E-5 0.0 1.5680537439274511 7.291068950909961E-5 86-87 2.9164275803639846E-4 7.291068950909961E-5 0.0 1.8828456458829885 7.291068950909961E-5 88-89 2.9164275803639846E-4 7.291068950909961E-5 0.0 2.2546172516898872 7.291068950909961E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9005 0.0 32.28172 1 GTATCAA 12945 0.0 29.211756 1 TAAGGTG 725 0.0 26.857565 5 GTACATG 9335 0.0 24.14035 1 GTAAGGT 710 0.0 24.082222 4 TATCAAC 16160 0.0 23.223795 2 ATCAACG 16240 0.0 23.163673 3 TCAACGC 16290 0.0 23.150045 4 TACATGG 9630 0.0 22.938982 2 CAACGCA 16470 0.0 22.866535 5 GAGTACT 9235 0.0 22.781778 12-13 AACGCAG 16800 0.0 22.44564 6 GTACTTT 9500 0.0 22.071297 14-15 ACATGGG 9550 0.0 21.883541 3 AGTACTT 9400 0.0 21.725079 12-13 AGGTAAG 620 0.0 21.454273 2 GGTAAGG 675 0.0 21.109879 3 ACGCAGA 18695 0.0 20.145058 7 CGCAGAG 18920 0.0 19.880386 8 AGAGTAC 15445 0.0 19.771692 10-11 >>END_MODULE