FastQCFastQC Report
Thu 26 May 2016
SRR936717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421317
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82900.5832618620617357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66130.46527270130449433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40050.28178091164743685No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23360.16435460914067726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.12284381316764663No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14710.10349556080733573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82100.030.2707631
GTATCAA119000.030.1085051
TAGCGCA700.00837610327.153569
TATAGCG908.922513E-426.4020825
GTACATG97950.026.0031031
TATCTCG950.001223127825.01255
TACATGG100000.024.8073982
TATCAAC143750.024.7288782
ATCAACG144300.024.6016923
TCAACGC145750.024.293444
CAACGCA146950.024.093365
AACGCAG150250.023.6574126
GAGTACT74050.023.55163812-13
ACATGGG101500.023.3638943
GTACTTT79000.022.19545614-15
CCGTCCA1754.0365358E-621.7228499
AGTACTT76800.021.62549412-13
CATGGGG37200.021.463854
GTATAGG3800.021.258381
ACGCAGA166200.021.2162367
Produced by FastQC (version 0.11.5)