##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936717_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1421317 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.395648542865526 31.0 31.0 34.0 30.0 34.0 2 31.428719279372583 31.0 31.0 34.0 28.0 34.0 3 31.444925375549577 31.0 31.0 34.0 28.0 34.0 4 35.11196024532177 37.0 35.0 37.0 32.0 37.0 5 35.09988412155768 37.0 35.0 37.0 32.0 37.0 6 35.217558785267464 37.0 35.0 37.0 32.0 37.0 7 35.09111901145206 37.0 35.0 37.0 32.0 37.0 8 35.04315997064694 37.0 35.0 37.0 32.0 37.0 9 36.52025269521155 39.0 37.0 39.0 32.0 39.0 10-11 36.41851430750494 39.0 37.0 39.0 32.0 39.0 12-13 36.30576887492375 39.0 35.0 39.0 32.0 39.0 14-15 37.31902946351869 39.5 37.0 41.0 32.0 41.0 16-17 37.20384192970323 39.0 36.0 41.0 31.0 41.0 18-19 37.198397683275445 39.0 36.5 41.0 31.0 41.0 20-21 37.13849795647276 39.0 36.0 41.0 31.0 41.0 22-23 36.992339850997354 39.0 36.0 41.0 31.0 41.0 24-25 37.10049552633227 39.0 36.0 41.0 31.0 41.0 26-27 37.057624724111506 39.0 36.0 41.0 31.0 41.0 28-29 36.94608767783683 39.0 36.0 41.0 30.5 41.0 30-31 36.70805668264012 39.0 36.0 41.0 30.0 41.0 32-33 36.4774195341363 39.0 35.0 41.0 30.0 41.0 34-35 36.2348086317127 39.0 35.0 40.0 30.0 41.0 36-37 36.02273947331946 38.0 35.0 40.0 29.5 41.0 38-39 35.794119116284406 38.0 35.0 40.0 28.5 41.0 40-41 35.57428919797624 38.0 35.0 40.0 27.5 41.0 42-43 35.29548123325057 38.0 34.5 40.0 26.5 41.0 44-45 34.95827672503741 38.0 34.0 40.0 25.5 41.0 46-47 34.56236856380385 38.0 34.0 40.0 24.0 41.0 48-49 34.382310561261136 38.0 34.0 40.0 23.0 41.0 50-51 33.42883360995471 37.5 32.0 39.5 22.0 40.5 52-53 33.556367791280906 37.5 33.0 39.5 22.0 40.5 54-55 33.96011938223492 38.0 33.0 40.0 22.0 41.0 56-57 33.86279626571694 38.0 33.0 40.0 21.5 41.0 58-59 33.60231179954929 37.5 33.0 40.0 20.0 41.0 60-61 33.275155014680045 37.0 33.0 40.0 18.5 41.0 62-63 32.94537495857715 36.5 32.0 40.0 18.0 41.0 64-65 32.59516349976818 36.0 32.0 39.0 15.5 41.0 66-67 32.21864650883652 35.5 31.5 39.0 13.0 41.0 68-69 31.81352506161539 35.0 31.0 39.0 9.5 40.0 70-71 31.377863629295927 35.0 31.0 38.0 7.5 40.0 72-73 30.992687415967023 35.0 30.5 37.0 7.0 39.5 74-75 30.574219544267745 35.0 30.0 37.0 3.5 39.0 76-77 30.135931322850567 34.5 29.5 36.0 2.0 39.0 78-79 29.69581697819698 34.0 29.0 36.0 2.0 38.5 80-81 29.24416544655415 34.0 29.0 35.0 2.0 37.0 82-83 28.761269653427068 34.0 29.0 35.0 2.0 37.0 84-85 28.447488843094117 34.0 28.0 35.0 2.0 36.0 86-87 28.131611033991714 34.0 27.0 35.0 2.0 36.0 88-89 27.725637560093915 34.0 26.5 35.0 2.0 35.5 90-91 27.312801085190706 33.0 26.0 35.0 2.0 35.0 92-93 26.95837592880406 33.0 25.5 35.0 2.0 35.0 94-95 26.501481372557986 33.0 25.0 35.0 2.0 35.0 96-97 25.915425974641828 33.0 23.5 35.0 2.0 35.0 98-99 25.223910288837747 32.5 19.0 35.0 2.0 35.0 100-101 22.829134528046872 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4042635606013434 1101 2 0.3578005377561091 1101 3 0.37295534937557306 1101 4 0.40749117220875775 1101 5 0.4430574296180154 1101 6 0.38345415074304157 1101 7 0.37785111633688473 1101 8 0.44261183168372753 1101 9 0.5970466991631724 1101 10-11 0.4731235232459525 1101 12-13 0.5923592878389741 1101 14-15 0.8010868785985394 1101 16-17 0.822364483482815 1101 18-19 0.7173451458643854 1101 20-21 0.8435722464203579 1101 22-23 0.730602760015536 1101 24-25 0.7388971601869869 1101 26-27 0.607993568875159 1101 28-29 0.7412017996773201 1101 30-31 0.7688063962003326 1101 32-33 0.6739700043963737 1101 34-35 0.6912741608237667 1101 36-37 0.5582959553973907 1101 38-39 0.5783184493256357 1101 40-41 0.44738830271897 1101 42-43 0.48105222327312447 1101 44-45 0.3899682823085513 1101 46-47 0.6623416127833295 1101 48-49 0.49611565253383105 1101 50-51 0.4840571616258842 1101 52-53 0.650972268199574 1101 54-55 0.6607227285197013 1101 56-57 0.4171774610359691 1101 58-59 0.5442437246084992 1101 60-61 0.36228769855156884 1101 62-63 0.4059397911617779 1101 64-65 0.3733208858331736 1101 66-67 0.3607837617472214 1101 68-69 0.44898799107759046 1101 70-71 0.3493166554610596 1101 72-73 0.2699320371177407 1101 74-75 0.36531270552837114 1101 76-77 0.2950632908988453 1101 78-79 0.19514798824137003 1101 80-81 0.40639616014429336 1101 82-83 0.392007586846308 1101 84-85 0.4694509551801893 1101 86-87 0.24946727176397943 1101 88-89 0.31006720734124116 1101 90-91 0.3481777303579392 1101 92-93 0.5184252518473222 1101 94-95 0.4859753259333104 1101 96-97 0.46220788508430033 1101 98-99 0.497969600072512 1101 100-101 0.9715027182829985 1102 1 0.4302601681630769 1102 2 0.4846044783653163 1102 3 0.3281074836270079 1102 4 0.34424500394045054 1102 5 0.4489437514078105 1102 6 0.3670063951331173 1102 7 0.34807217131372425 1102 8 0.1526434860333694 1102 9 0.30209216288047713 1102 10-11 0.34249836035007064 1102 12-13 0.3669048083071047 1102 14-15 0.5509108459436902 1102 16-17 0.4087289940384693 1102 18-19 0.6224354322248473 1102 20-21 0.6217792461092841 1102 22-23 0.2259951709587753 1102 24-25 0.26385070858493265 1102 26-27 0.2742527330826192 1102 28-29 0.4681769911588134 1102 30-31 0.3899951578552674 1102 32-33 0.4345493042172919 1102 34-35 0.3233178316353431 1102 36-37 0.4747046159938151 1102 38-39 0.337427482375638 1102 40-41 0.4698235332113967 1102 42-43 0.6811275232117495 1102 44-45 0.5712183348556081 1102 46-47 0.5537076577131685 1102 48-49 0.7864171465518766 1102 50-51 0.7681349817215661 1102 52-53 0.5708839786274424 1102 54-55 0.6687534958749524 1102 56-57 0.6930713390931587 1102 58-59 0.759888718765275 1102 60-61 0.5889289534594582 1102 62-63 0.7639342484977689 1102 64-65 0.6986126796637322 1102 66-67 0.6488047133114421 1102 68-69 0.43069346900361793 1102 70-71 0.39541724314739213 1102 72-73 0.5555027192206339 1102 74-75 0.49584932662622805 1102 76-77 0.5505731129955151 1102 78-79 0.3802917780194939 1102 80-81 0.43885973452150395 1102 82-83 0.6041437509949219 1102 84-85 0.5356192655189247 1102 86-87 0.4048491943138153 1102 88-89 0.5147414260715344 1102 90-91 0.4275663558108924 1102 92-93 0.3829442150307507 1102 94-95 0.6079197143275543 1102 96-97 0.25150494202850027 1102 98-99 0.43856015547392957 1102 100-101 0.36165177327665177 1103 1 0.1590083747627915 1103 2 0.26316224293455903 1103 3 0.13209737226539175 1103 4 0.16419615513263608 1103 5 0.29164973624077106 1103 6 0.1336252377921312 1103 7 0.10428075230709766 1103 8 0.18482221118195952 1103 9 0.3731236242455154 1103 10-11 0.2772527499107724 1103 12-13 0.17014958182627282 1103 14-15 0.12204265232477951 1103 16-17 0.30270060707393043 1103 18-19 0.1620354185608477 1103 20-21 0.21654337127154832 1103 22-23 0.2882218909150538 1103 24-25 0.3429311212377968 1103 26-27 0.2196479643458531 1103 28-29 -0.10833501608311025 1103 30-31 -0.09334116447853802 1103 32-33 0.16736468646775648 1103 34-35 -0.10611613707409617 1103 36-37 -0.09266745478571181 1103 38-39 0.04569676569872172 1103 40-41 0.07582416517150392 1103 42-43 0.10153471047689777 1103 44-45 0.13981376083088293 1103 46-47 0.2960963814953672 1103 48-49 0.2639975079647314 1103 50-51 0.20341031136561583 1103 52-53 0.12688076758691835 1103 54-55 0.1360666406450548 1103 56-57 0.2565747892537118 1103 58-59 0.013153134287577473 1103 60-61 0.1454701675060548 1103 62-63 0.1944656073827815 1103 64-65 0.4020803472185577 1103 66-67 0.2800132943048155 1103 68-69 0.28527859573724257 1103 70-71 0.2700905880951261 1103 72-73 0.29129429194728473 1103 74-75 0.329167714957169 1103 76-77 0.2668208925951596 1103 78-79 0.3767797024575614 1103 80-81 0.36923523838158445 1103 82-83 0.32693530664156967 1103 84-85 0.3637666830225115 1103 86-87 0.35730914170740746 1103 88-89 0.2637102209065425 1103 90-91 0.3309832103575907 1103 92-93 0.16438423006114888 1103 94-95 0.3374706810557093 1103 96-97 0.1905955172829934 1103 98-99 0.38799267238313107 1103 100-101 0.9565589434618005 1104 1 0.11974829140072885 1104 2 0.2270794427393632 1104 3 0.1559453238061579 1104 4 0.30533664169487196 1104 5 0.29343970369285444 1104 6 0.10594691819578372 1104 7 0.23993521387861705 1104 8 0.1992555551109021 1104 9 0.4379821728860662 1104 10-11 0.4149817493421821 1104 12-13 0.1565117478967437 1104 14-15 0.3316620554936094 1104 16-17 0.14442839435293564 1104 18-19 0.2730518749569413 1104 20-21 0.4702426466662999 1104 22-23 0.12785527357019077 1104 24-25 0.2503799825084627 1104 26-27 -0.1399974055229194 1104 28-29 0.1073262617471471 1104 30-31 0.18904879603417157 1104 32-33 0.00676414497251443 1104 34-35 -0.10360550975444482 1104 36-37 5.1292276246783786E-5 1104 38-39 0.0715991085218377 1104 40-41 0.185123825365892 1104 42-43 0.3018606653127449 1104 44-45 0.26321868353517885 1104 46-47 0.15374877916194407 1104 48-49 0.0908089930808913 1104 50-51 0.21279022209389353 1104 52-53 0.1426593152873039 1104 54-55 0.28752301435064 1104 56-57 0.21675325994939243 1104 58-59 0.19042203065806262 1104 60-61 0.39006654761377035 1104 62-63 0.33234948362907346 1104 64-65 0.10632047427630908 1104 66-67 0.45665736695784886 1104 68-69 0.24683822624900742 1104 70-71 0.27171504581641415 1104 72-73 0.17468967860487794 1104 74-75 -0.1329334947328853 1104 76-77 0.10080665791887 1104 78-79 0.20935730595500956 1104 80-81 0.2690582003314148 1104 82-83 0.26595245585607685 1104 84-85 0.34527758120171015 1104 86-87 0.4072343195569168 1104 88-89 0.31965642730598987 1104 90-91 0.23824011951443325 1104 92-93 0.15216057394979998 1104 94-95 0.18478704500139642 1104 96-97 0.15118769235227703 1104 98-99 0.4436794915967006 1104 100-101 0.6157432085370331 1105 1 0.3072234747562774 1105 2 0.2966250466607008 1105 3 0.2812244649629356 1105 4 0.3667766059176003 1105 5 0.4764300437125968 1105 6 0.2618538548203375 1105 7 0.3703505523201258 1105 8 0.41030426446839385 1105 9 0.47259597132388365 1105 10-11 0.5001250383610341 1105 12-13 0.28788213996580225 1105 14-15 0.16896752692578332 1105 16-17 0.2410739215159836 1105 18-19 0.20457947106557128 1105 20-21 0.22327949595397456 1105 22-23 0.10327669190521505 1105 24-25 0.28399359273802816 1105 26-27 0.3503988111962286 1105 28-29 0.17203759807883046 1105 30-31 0.15388010775127725 1105 32-33 0.13338685114756998 1105 34-35 0.09094960782852013 1105 36-37 0.14890882688974472 1105 38-39 -0.026651941225907194 1105 40-41 -0.05356479971678141 1105 42-43 0.3223041228930299 1105 44-45 -0.142417445605183 1105 46-47 -0.012120509912357136 1105 48-49 0.09881079374415691 1105 50-51 0.08658567453414179 1105 52-53 0.06320209844484737 1105 54-55 0.11531880662772664 1105 56-57 0.3349552693527258 1105 58-59 0.2202284062568296 1105 60-61 0.0490534031258818 1105 62-63 0.2911225841269669 1105 64-65 0.5472785427758495 1105 66-67 0.5223427842403154 1105 68-69 0.4487675378257059 1105 70-71 0.41152884944617796 1105 72-73 0.4085484610567889 1105 74-75 0.4018950617098227 1105 76-77 0.12300697167795605 1105 78-79 0.17157523695043153 1105 80-81 0.07622704673282854 1105 82-83 0.23294712997555322 1105 84-85 0.43823048016927757 1105 86-87 0.36994268349621606 1105 88-89 0.2939403362084754 1105 90-91 0.36066368719948727 1105 92-93 0.3808644267910033 1105 94-95 0.458578426479459 1105 96-97 0.748767623334718 1105 98-99 0.7994886661294665 1105 100-101 1.344685124166638 1106 1 0.27429933927247774 1106 2 0.23533111346073454 1106 3 0.21866976179297026 1106 4 0.17670704096403966 1106 5 0.26147189679051763 1106 6 0.15192876229902197 1106 7 0.4222449391730265 1106 8 0.2341143818918212 1106 9 0.5110008120473779 1106 10-11 0.3571481833670589 1106 12-13 0.504782228810015 1106 14-15 0.5850941698347043 1106 16-17 0.6591779582883817 1106 18-19 0.5780463807181917 1106 20-21 0.4347960013885128 1106 22-23 0.3723585756663752 1106 24-25 0.4858808562241137 1106 26-27 0.08417864111925155 1106 28-29 0.46311732900024793 1106 30-31 0.307820022152157 1106 32-33 0.3343925979897122 1106 34-35 0.21824582182500762 1106 36-37 0.1996349486056559 1106 38-39 0.33832480559841827 1106 40-41 0.12464023716313477 1106 42-43 -0.11639353437672639 1106 44-45 0.2346670460997231 1106 46-47 0.2729355239421025 1106 48-49 0.4038881053591368 1106 50-51 -0.07519752843499106 1106 52-53 0.2851909424609218 1106 54-55 0.267015684944127 1106 56-57 0.2934766628679526 1106 58-59 0.31766831439178134 1106 60-61 0.3212067441498192 1106 62-63 0.4491213335803437 1106 64-65 0.3345734903960178 1106 66-67 0.43169546806770853 1106 68-69 0.4615116038302922 1106 70-71 0.5307075582304108 1106 72-73 0.5421040038606364 1106 74-75 0.3759020280987464 1106 76-77 0.5828387678810678 1106 78-79 0.6314360542776214 1106 80-81 0.6832500268461068 1106 82-83 0.9449380318531553 1106 84-85 0.7096866546442762 1106 86-87 0.8190401265373133 1106 88-89 1.150900162398326 1106 90-91 0.8573938639579239 1106 92-93 0.9497534849697864 1106 94-95 1.0977089931762727 1106 96-97 1.3164219645980708 1106 98-99 1.513087767002279 1106 100-101 1.6961304421029482 1107 1 0.13896280479027112 1107 2 0.10732765895312468 1107 3 3.5144654063401504E-5 1107 4 0.17769042037273408 1107 5 0.31593146253270277 1107 6 0.10973361431837958 1107 7 0.21586910438358586 1107 8 0.2560153543173982 1107 9 0.3055439016409167 1107 10-11 0.30177673750699086 1107 12-13 0.3470683534744907 1107 14-15 0.3446163432440841 1107 16-17 0.3744554547847443 1107 18-19 0.49075807409261074 1107 20-21 0.47107660828012143 1107 22-23 0.49874308194616646 1107 24-25 0.49983941974348056 1107 26-27 0.44352210152290183 1107 28-29 0.4325698733711931 1107 30-31 0.47484747259663607 1107 32-33 0.3055963466658298 1107 34-35 0.5003681109716638 1107 36-37 0.4873213266523493 1107 38-39 0.33881244485033335 1107 40-41 0.4102441543380735 1107 42-43 0.5915366175081971 1107 44-45 0.5720894579222531 1107 46-47 0.900851101242516 1107 48-49 0.9045905946967636 1107 50-51 0.7422783423154229 1107 52-53 0.8460646637947278 1107 54-55 0.8836937222287204 1107 56-57 0.9025833479107632 1107 58-59 0.9290210633634075 1107 60-61 0.7902317016288691 1107 62-63 0.9358987196578497 1107 64-65 1.009772640575882 1107 66-67 0.9448615517627346 1107 68-69 0.9699272112449471 1107 70-71 1.0250670390539796 1107 72-73 0.929605152956384 1107 74-75 0.8398883988546189 1107 76-77 0.93514870568373 1107 78-79 0.9914797146327459 1107 80-81 0.9763189278597402 1107 82-83 0.9881810142682035 1107 84-85 0.8667572124690643 1107 86-87 0.7167531706495573 1107 88-89 0.6583013956378174 1107 90-91 0.6491648591259107 1107 92-93 0.6659934041861533 1107 94-95 0.7782887291501694 1107 96-97 0.8266581623291849 1107 98-99 0.9420511082944536 1107 100-101 1.1502319478498322 1108 1 -0.17236545917445412 1108 2 0.013369435903491933 1108 3 -0.0660176535614525 1108 4 0.061895831333394824 1108 5 0.13308929371513756 1108 6 -0.038937594680305665 1108 7 -0.24779792319980487 1108 8 -0.1000536657743254 1108 9 0.047011528030878935 1108 10-11 -0.20915442763914882 1108 12-13 0.049654824290222166 1108 14-15 0.11999722406173419 1108 16-17 0.17617557045377907 1108 18-19 0.08162286363062776 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90-91 -2.9645502204060215 2316 92-93 -3.035172766650579 2316 94-95 -3.357770458878523 2316 96-97 -4.071370138544555 2316 98-99 -5.590405208508354 2316 100-101 -5.830662466841453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3060.0 3 1187.0 4 1236.0 5 1450.0 6 1623.0 7 1938.0 8 2348.0 9 3161.0 10 3967.0 11 4978.0 12 6531.0 13 8096.0 14 9466.0 15 11866.0 16 14981.0 17 19186.0 18 15445.0 19 10308.0 20 10094.0 21 10477.0 22 11651.0 23 13094.0 24 15099.0 25 17293.0 26 19754.0 27 23372.0 28 28012.0 29 33843.0 30 40839.0 31 49824.0 32 61932.0 33 78303.0 34 103103.0 35 139705.0 36 190318.0 37 241225.0 38 184774.0 39 27770.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.94525317547066 18.762286099578198 14.752117273701748 21.54034345124939 2 15.628831009803163 20.28107560328508 40.297754350901144 23.792339036010613 3 19.320446561275503 25.632656622978878 29.913257780480112 25.133639035265503 4 12.525905130905384 16.602513924007862 37.354354033034674 33.51722691205208 5 13.179330896158175 38.31173173896178 33.383667828935934 15.125269535944112 6 30.976452070686022 36.08784225168099 17.587456497009697 15.348249180623288 7 28.31269833671697 34.158827057422265 20.044925818196152 17.483548787664617 8 27.441938712733716 33.286641222986034 19.46103695903436 19.810383105245894 9 27.539318756440505 16.558503719303776 20.443219379796947 35.45895814445878 10-11 25.214421273744026 26.76288639473788 26.345975694229352 21.676716637288745 12-13 26.726110031815754 24.523362051975113 27.175824253174536 21.574703663034605 14-15 23.43821979597518 25.332806384758566 26.604521458080317 24.624452361185945 16-17 21.645665985160793 28.951728055575405 27.109656043082342 22.292949916181463 18-19 21.66936686530696 26.723026493858647 30.44595689983172 21.161649741002673 20-21 22.877365709599417 25.398643154962002 30.474058421808316 21.249932713630265 22-23 22.3303608122639 25.70389777455004 30.45447764134957 21.51126377183649 24-25 22.0036644160435 25.88356191371454 30.656414557469695 21.456359112772265 26-27 21.601936709346184 26.050373159927343 30.647454374852895 21.700235755873578 28-29 21.387525637913505 26.389724429809284 30.682751269142685 21.539998663134526 30-31 21.546066808544023 25.949307635448136 30.61732834077023 21.887297215237613 32-33 21.290115358866302 26.258134944297684 30.42570309946699 22.026046597369024 34-35 22.160967516320646 26.089602773982097 30.18971211773663 21.55971759196063 36-37 22.271742660094223 25.709679904783783 30.254896173021628 21.763681262100366 38-39 21.870060144635623 26.062311883095298 30.19580869819144 21.871819274077644 40-41 21.93785313318246 26.11693072758672 30.243872751213768 21.70134338801705 42-43 21.841789668058944 26.283067196278875 29.684375091246235 22.190768044415943 44-45 21.86408336692974 26.3334958625411 29.622953345674297 22.179467424854867 46-47 22.31564512991319 26.688498569458062 28.970576181415165 22.025280119213587 48-49 22.344946030175823 26.92778143443677 28.457152184596744 22.270120350790666 50-51 22.207236063311946 27.256797682985045 28.39581611771945 22.14015013598356 52-53 22.612839221259858 27.21446312101149 28.125584864241137 22.047112793487514 54-55 21.904888556779152 27.163761761926576 28.355733149828634 22.575616531465638 56-57 22.30234558757558 27.40957973643146 27.850338388787343 22.437736287205613 58-59 22.542554995528306 26.843475158690676 28.03549351551302 22.578476330268 60-61 22.193372611023506 27.054321003290966 28.63552597414934 22.116780411536187 62-63 22.177280901975465 27.104787663695546 28.631029610845772 22.08690182348322 64-65 21.895783980632046 27.242163430114463 28.454911888058753 22.407140701194734 66-67 21.97203037811515 27.337066978698942 28.044135304831304 22.646767338354604 68-69 22.085682780478777 27.079986404696715 28.4534146236768 22.380916191147712 70-71 22.077518638042893 27.211243583415513 28.11226555785366 22.598972220687937 72-73 22.12453233094874 27.352892028474386 27.94426141270793 22.578314227868944 74-75 21.956307752492684 27.953807152672383 27.745822541844944 22.34406255298999 76-77 22.18244005820384 27.479186778608682 27.735658015834375 22.602715147353102 78-79 22.196227487778174 27.57400642261481 27.74122097228594 22.488545117321078 80-81 22.266888693350914 27.87649865373161 27.462538274447358 22.39407437847012 82-83 22.311680233715848 27.794742941416946 27.543513885673715 22.350062939193492 84-85 22.09918838954065 27.788554135687814 27.377629500722524 22.734627974049012 86-87 22.29690936646445 27.706397553723384 27.399346496282885 22.597346583529283 88-89 22.186482507260425 27.538773564494196 27.289518076739732 22.985225851505646 90-91 22.04770254125547 27.561554276257926 27.58642646767037 22.804316714816228 92-93 22.25929542155957 27.57584553810969 27.597308352025333 22.567550688305403 94-95 21.958495496947183 27.320889394270964 27.725456972431424 22.995158136350426 96-97 21.960405451665007 27.217760164877014 27.722798054152868 23.099036329305108 98-99 21.801465155065763 27.524401657975666 27.38450116466457 23.289632022294 100-101 21.883261400204397 27.380585375192503 27.547784149936234 23.18836907466687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 400.0 1 295.5 2 274.5 3 379.0 4 372.5 5 375.5 6 587.5 7 1197.0 8 2019.5 9 2448.5 10 2234.5 11 1961.0 12 2007.5 13 2189.0 14 2371.5 15 2536.0 16 2630.0 17 2704.5 18 2925.0 19 3185.0 20 3464.0 21 3907.0 22 4471.0 23 5157.0 24 5943.0 25 7097.5 26 8928.5 27 11094.5 28 13013.5 29 15215.0 30 17786.5 31 20728.0 32 24692.5 33 27533.5 34 29443.5 35 31650.5 36 34814.5 37 37886.5 38 38867.0 39 39127.0 40 39638.0 41 41200.5 42 42531.5 43 43628.0 44 44972.0 45 47265.5 46 48569.5 47 50406.5 48 56945.5 49 56822.0 50 49997.5 51 46388.5 52 44808.0 53 44794.0 54 44778.5 55 42340.5 56 38792.5 57 35285.0 58 33188.0 59 30274.5 60 25581.0 61 21837.5 62 18848.5 63 14945.0 64 10660.0 65 8059.0 66 6214.5 67 4820.5 68 3564.5 69 2394.5 70 1792.0 71 1342.5 72 1074.5 73 1002.5 74 810.0 75 626.0 76 481.0 77 294.0 78 180.0 79 137.5 80 95.0 81 64.0 82 44.5 83 31.5 84 21.5 85 16.0 86 11.0 87 6.0 88 5.5 89 4.5 90 4.0 91 2.5 92 2.0 93 1.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03546006978035161 2 0.024413976614646837 3 0.022866116425821967 4 0.05269760370135585 5 0.05769297067438158 6 0.05100902894991054 7 0.05297903282659674 8 0.04664687750867681 9 0.04369187169364751 10-11 0.05269760370135585 12-13 0.044817588194611055 14-15 0.03929454161175867 16-17 0.048440988182087454 18-19 0.05224028137283942 20-21 0.01072948539980877 22-23 0.021177541674376653 24-25 0.005698939786127936 26-27 0.010096269868016776 28-29 0.005769297067438157 30-31 0.009603768898845226 32-33 0.0039751863940275115 34-35 0.007809658225434579 36-37 0.009181625210983898 38-39 0.01122198636898032 40-41 0.0015830388294799822 42-43 0.0017941106734106467 44-45 0.002392147564547529 46-47 1.758932032755536E-4 48-49 9.498232976879894E-4 50-51 3.8696504720621796E-4 52-53 0.0033067922215804075 54-55 0.007035728131022143 56-57 0.017202355280349142 58-59 0.010975735884394544 60-61 0.0108350213217741 62-63 0.005698939786127936 64-65 0.0 66-67 0.006789477646436368 68-69 0.016745032951832703 70-71 0.00685983492774659 72-73 0.002779112611753747 74-75 0.004080722315992843 76-77 0.007387514537573251 78-79 0.006261798036609707 80-81 0.011714487338151868 82-83 0.007317157256263029 84-85 0.017835570812141134 86-87 0.0038696504720621785 88-89 0.005945190270713711 90-91 0.0035882213468212934 92-93 0.017905928093451356 94-95 0.011960737822737644 96-97 0.009603768898845226 98-99 0.02159968536223798 100-101 0.03890757656455245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1421317.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.35924128031686 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32970451253318 40.72895119939548 2 12.980828972286842 13.852943703015622 3 4.505823931873591 7.212820390424067 4 2.053429235937039 4.382777042096846 5 1.103956288217967 2.945313497297276 6 0.6293669521687664 2.0149525832778505 7 0.4236272586361994 1.582310037454171 8 0.31125624219983783 1.328671754203671 9 0.23267017980568624 1.1173593836687685 >10 1.2741759785200344 12.686229777197624 >50 0.09970377465014679 3.6796167014261334 >100 0.0497790190597512 4.891419838111898 >500 0.0044893079017952284 1.6322246039464188 >1k 9.242692738990176E-4 0.8944227283247631 >5k 2.640769353997193E-4 1.0499867601594284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8290 0.5832618620617357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6613 0.46527270130449433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4005 0.28178091164743685 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2336 0.16435460914067726 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.12284381316764663 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1471 0.10349556080733573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.5178640655110716E-5 0.0 24-25 0.0 0.0 0.0 7.035728131022143E-5 0.0 26-27 0.0 0.0 0.0 3.1660776589599647E-4 0.0 28-29 0.0 0.0 0.0 8.091087350675464E-4 0.0 30-31 0.0 0.0 0.0 0.0022162543612719754 0.0 32-33 0.0 0.0 0.0 0.003728935909441736 0.0 34-35 0.0 0.0 0.0 0.00541751066088705 0.0 36-37 0.0 0.0 0.0 0.007915194147399912 0.0 38-39 0.0 0.0 0.0 0.012629131995184747 0.0 40-41 0.0 0.0 0.0 0.018539143625243348 0.0 42-43 0.0 0.0 0.0 0.026032194084781933 0.0 44-45 0.0 0.0 0.0 0.03254024260597741 0.0 46-47 0.0 0.0 0.0 0.04583776877360926 0.0 48-49 0.0 0.0 0.0 0.06402512599230151 0.0 50-51 0.0 0.0 0.0 0.07169406965511564 0.0 52-53 0.0 0.0 0.0 0.08618766960502126 0.0 54-55 0.0 0.0 0.0 0.11165700543932142 0.0 56-57 0.0 0.0 0.0 0.1507052965664943 0.0 58-59 7.035728131022143E-5 0.0 0.0 0.2021364692042662 0.0 60-61 7.035728131022143E-5 0.0 3.5178640655110716E-5 0.26032194084781934 0.0 62-63 7.035728131022143E-5 0.0 7.035728131022143E-5 0.3184018765694071 0.0 64-65 7.035728131022143E-5 0.0 7.035728131022143E-5 0.3799293190751957 0.0 66-67 7.035728131022143E-5 0.0 7.035728131022143E-5 0.465167165382529 0.0 68-69 7.035728131022143E-5 0.0 7.035728131022143E-5 0.5715825533642389 0.0 70-71 7.035728131022143E-5 0.0 7.035728131022143E-5 0.6373314327486408 0.0 72-73 7.035728131022143E-5 0.0 7.035728131022143E-5 0.7192624868343938 0.0 74-75 7.035728131022143E-5 0.0 7.035728131022143E-5 0.8014046127640773 0.0 76-77 7.035728131022143E-5 0.0 7.035728131022143E-5 0.8948742609847065 0.0 78-79 7.035728131022143E-5 0.0 7.035728131022143E-5 1.0038576897342395 0.0 80-81 7.035728131022143E-5 0.0 7.035728131022143E-5 1.1607896056966882 0.0 82-83 7.035728131022143E-5 0.0 7.035728131022143E-5 1.3247572497901594 0.0 84-85 1.0553592196533215E-4 0.0 7.035728131022143E-5 1.5677361207950091 0.0 86-87 1.4071456262044286E-4 0.0 7.035728131022143E-5 1.8824090614549744 0.0 88-89 1.4071456262044286E-4 7.035728131022143E-5 7.035728131022143E-5 2.246050669906854 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8210 0.0 30.270763 1 GTATCAA 11900 0.0 30.108505 1 TAGCGCA 70 0.008376103 27.15356 9 TATAGCG 90 8.922513E-4 26.402082 5 GTACATG 9795 0.0 26.003103 1 TATCTCG 95 0.0012231278 25.0125 5 TACATGG 10000 0.0 24.807398 2 TATCAAC 14375 0.0 24.728878 2 ATCAACG 14430 0.0 24.601692 3 TCAACGC 14575 0.0 24.29344 4 CAACGCA 14695 0.0 24.09336 5 AACGCAG 15025 0.0 23.657412 6 GAGTACT 7405 0.0 23.551638 12-13 ACATGGG 10150 0.0 23.363894 3 GTACTTT 7900 0.0 22.195456 14-15 CCGTCCA 175 4.0365358E-6 21.722849 9 AGTACTT 7680 0.0 21.625494 12-13 CATGGGG 3720 0.0 21.46385 4 GTATAGG 380 0.0 21.25838 1 ACGCAGA 16620 0.0 21.216236 7 >>END_MODULE