FastQCFastQC Report
Thu 26 May 2016
SRR936717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421317
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98590.6936524364374731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88310.6213251512505654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.38457289964167035No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27290.1920050206955943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.1624549625453013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.10398806177650728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92500.033.1827551
GTATCAA134600.029.2638281
GTAAGGT6600.028.7842124
TAAGGTG7300.028.6264885
GGTAAGG6400.028.2005233
GTACATG97650.025.4478511
TGCACCG3450.024.7794515
GTATAGG5000.024.7072851
TACATGG99050.024.4088572
TATCAAC166150.023.613692
AGGTAAG6650.023.5709132
ATCAACG167050.023.4848143
TCAACGC167950.023.3581434
GAGTACT97850.023.27905712-13
CAACGCA169250.023.1506715
ACATGGG99100.023.1007963
AACGCAG172950.022.657796
GTACTTT103150.022.19690914-15
AGTACTT99500.021.6278212-13
TACCTGG11100.021.395932
Produced by FastQC (version 0.11.5)