##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936717_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1421317 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79871344675396 31.0 31.0 34.0 30.0 34.0 2 31.9349976113703 31.0 31.0 34.0 30.0 34.0 3 32.01669930071898 31.0 31.0 34.0 30.0 34.0 4 35.79793318450423 37.0 35.0 37.0 35.0 37.0 5 35.57169653215996 37.0 35.0 37.0 33.0 37.0 6 35.682818822261325 37.0 35.0 37.0 33.0 37.0 7 35.62431041069656 37.0 35.0 37.0 33.0 37.0 8 35.59656572038469 37.0 35.0 37.0 33.0 37.0 9 37.19636787570964 39.0 37.0 39.0 33.0 39.0 10-11 37.07052437985333 39.0 37.0 39.0 33.0 39.0 12-13 36.96481784148082 39.0 37.0 39.0 33.0 39.0 14-15 38.00660584514222 40.0 37.0 41.0 33.0 41.0 16-17 37.93580707189177 40.0 37.0 41.0 33.0 41.0 18-19 38.130642917800884 40.0 37.0 41.0 33.0 41.0 20-21 38.17070611271096 40.0 37.0 41.0 33.0 41.0 22-23 38.08257587856896 40.0 37.0 41.0 33.0 41.0 24-25 37.942822396411216 40.0 37.0 41.0 33.0 41.0 26-27 37.709579214207665 40.0 37.0 41.0 32.0 41.0 28-29 37.60008675052785 39.0 37.0 41.0 32.0 41.0 30-31 37.50747933078968 39.0 36.5 41.0 32.0 41.0 32-33 37.30896063299039 39.0 36.0 41.0 32.0 41.0 34-35 37.09628675376429 39.0 35.0 41.0 31.0 41.0 36-37 36.79416836638132 39.0 35.0 41.0 31.0 41.0 38-39 36.60008499159582 39.0 35.0 41.0 30.0 41.0 40-41 36.33492071086182 39.0 35.0 40.0 30.0 41.0 42-43 36.027331341284174 38.5 35.0 40.0 30.0 41.0 44-45 35.67714767360131 38.0 35.0 40.0 28.5 41.0 46-47 35.170776821778674 38.0 34.5 40.0 26.5 41.0 48-49 34.7036417632379 38.0 34.0 40.0 24.5 41.0 50-51 34.33379323542883 38.0 33.5 40.0 23.5 41.0 52-53 34.026353023287555 38.0 33.0 40.0 23.0 41.0 54-55 33.74588849637343 38.0 33.0 40.0 22.0 41.0 56-57 33.4177818882065 37.0 33.0 40.0 20.5 41.0 58-59 33.06523808552209 37.0 32.0 40.0 19.5 41.0 60-61 32.689674435752195 36.0 32.0 39.0 18.0 40.5 62-63 32.28772012154924 36.0 31.0 39.0 16.5 40.0 64-65 31.863949773344018 35.0 31.0 39.0 13.5 40.0 66-67 31.393499127921498 35.0 30.5 38.0 9.5 40.0 68-69 31.105595373868038 35.0 30.0 38.0 8.0 40.0 70-71 30.663993676287554 34.5 30.0 37.0 7.0 39.5 72-73 30.21568446729336 34.0 29.0 36.5 6.0 39.0 74-75 29.683704972219427 34.0 29.0 36.0 2.0 39.0 76-77 28.29922353704346 32.5 27.0 34.5 2.0 36.5 78-79 29.07776625481859 34.0 29.0 35.0 2.0 37.0 80-81 28.89249125986673 34.0 29.0 35.0 2.0 37.0 82-83 28.548240469930356 34.0 28.5 35.0 2.0 36.5 84-85 28.12694810517288 33.0 27.0 35.0 2.0 36.0 86-87 27.738648732126613 33.0 27.0 35.0 2.0 35.5 88-89 27.315722319510705 33.0 26.0 35.0 2.0 35.0 90-91 26.818332574647314 33.0 25.0 35.0 2.0 35.0 92-93 26.304044769745243 32.0 25.0 34.0 2.0 35.0 94-95 25.690665769845857 32.0 23.5 34.0 2.0 35.0 96-97 24.898159946021895 32.0 19.0 34.0 2.0 35.0 98-99 23.93902767644375 31.0 4.5 34.0 2.0 35.0 100-101 20.806873132453916 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2875316899393461 1101 2 0.2904282758939196 1101 3 0.19808183436699878 1101 4 0.13151691143602307 1101 5 0.13303781268378145 1101 6 0.10306142911856853 1101 7 0.05553431956101207 1101 8 0.18524696721197387 1101 9 0.26926713112164435 1101 10-11 0.2836788855875412 1101 12-13 0.2873086513557084 1101 14-15 0.3411612038638836 1101 16-17 0.436782572012163 1101 18-19 0.3850287143508595 1101 20-21 0.3602126387004816 1101 22-23 0.2903933068940532 1101 24-25 0.23154497204421887 1101 26-27 0.2881408111934647 1101 28-29 0.25768843710851996 1101 30-31 0.24831976063636318 1101 32-33 0.15818678500298944 1101 34-35 0.10813157091596537 1101 36-37 0.03186598193438073 1101 38-39 0.2389339889193849 1101 40-41 0.24447512650984038 1101 42-43 0.547578452508894 1101 44-45 0.3405367547743978 1101 46-47 0.5037191360695132 1101 48-49 0.50992796428541 1101 50-51 0.32372106840848147 1101 52-53 0.4276476990710876 1101 54-55 0.4673981381996555 1101 56-57 0.4681708089950902 1101 58-59 0.4412825804437688 1101 60-61 0.3870539641594988 1101 62-63 0.5204539601538656 1101 64-65 0.3494684579279017 1101 66-67 0.18193418774412962 1101 68-69 0.24838140742224013 1101 70-71 0.17830107745081492 1101 72-73 0.17056475221092882 1101 74-75 0.10524317161688757 1101 76-77 0.013944783492380708 1101 78-79 -0.14895683408764882 1101 80-81 0.04779654891496321 1101 82-83 0.41234596449178795 1101 84-85 0.24945344993106033 1101 86-87 0.26413544810099054 1101 88-89 0.28400194606401996 1101 90-91 0.40888091377942715 1101 92-93 0.3572036772674956 1101 94-95 0.2985824206422194 1101 96-97 0.10960329488361964 1101 98-99 0.15047130910015838 1101 100-101 0.7211868293858927 1102 1 0.259422690519461 1102 2 0.2848772043542489 1102 3 0.3064054990410803 1102 4 0.14916274004011854 1102 5 0.07035389796975977 1102 6 0.19193112081445207 1102 7 0.31940937105712663 1102 8 0.2211550014474355 1102 9 0.2553989107434731 1102 10-11 0.2862844316145612 1102 12-13 0.21570855130610056 1102 14-15 0.4050332076304599 1102 16-17 0.3819208128206313 1102 18-19 0.3050528754895794 1102 20-21 0.18825133015254636 1102 22-23 0.14160236222400613 1102 24-25 0.19266829158920018 1102 26-27 0.020509695766492086 1102 28-29 0.08632196129277503 1102 30-31 0.18206395944081066 1102 32-33 0.1671274047220166 1102 34-35 0.1203224891022856 1102 36-37 0.22626646200432532 1102 38-39 0.2267924649705364 1102 40-41 0.3009579625377867 1102 42-43 0.3725554894431724 1102 44-45 0.3591095135371489 1102 46-47 0.3003013175712681 1102 48-49 0.2621543832662496 1102 50-51 0.14242030794741112 1102 52-53 0.11398141704890463 1102 54-55 0.3359986943577482 1102 56-57 0.28451252463562327 1102 58-59 0.14341982706184098 1102 60-61 0.15034285318851204 1102 62-63 0.0613967910532196 1102 64-65 0.1497344511514349 1102 66-67 0.1887731877525347 1102 68-69 0.08778655367122212 1102 70-71 0.30023101540324504 1102 72-73 0.298518685253363 1102 74-75 0.3112612814360425 1102 76-77 0.12490631063608859 1102 78-79 0.2096429653927636 1102 80-81 0.4036770379809731 1102 82-83 0.39752217580663896 1102 84-85 0.37780936799127574 1102 86-87 0.5691461782108789 1102 88-89 0.3584648100049641 1102 90-91 0.5532694656664994 1102 92-93 0.6245573442116132 1102 94-95 0.7516455464777181 1102 96-97 0.8473711272542914 1102 98-99 0.9713142059986204 1102 100-101 1.127613124776044 1103 1 0.06282758906424846 1103 2 0.01743852156405623 1103 3 0.08733311398949795 1103 4 0.08777294021775361 1103 5 -0.21269163731752627 1103 6 0.03328778543151856 1103 7 0.05245746834482645 1103 8 0.008961587496486345 1103 9 0.10624778687929393 1103 10-11 0.05502973461381799 1103 12-13 0.057842356047515864 1103 14-15 0.0011957580164576598 1103 16-17 0.09476326687541814 1103 18-19 -0.047207235872043896 1103 20-21 -0.10801839725061058 1103 22-23 -0.1053275072847768 1103 24-25 -0.11510481765449754 1103 26-27 0.17806204597486186 1103 28-29 0.014538979807504404 1103 30-31 -0.11298241276661258 1103 32-33 -0.049624234958251634 1103 34-35 -0.09878192095441563 1103 36-37 0.1565971308572358 1103 38-39 0.1086330060650269 1103 40-41 0.19083037491736832 1103 42-43 0.08910643909132432 1103 44-45 0.21756753089043457 1103 46-47 0.38645498635585085 1103 48-49 0.3588613587393681 1103 50-51 0.43307284945181124 1103 52-53 0.2545733245436139 1103 54-55 0.2936744475858717 1103 56-57 0.2543513748098363 1103 58-59 0.5119977460343037 1103 60-61 0.36107806190614156 1103 62-63 0.38218059237862434 1103 64-65 0.2563563682109411 1103 66-67 0.2973294151215349 1103 68-69 0.3332881079514678 1103 70-71 0.20280454481500954 1103 72-73 0.17163988495275362 1103 74-75 0.12196788114910007 1103 76-77 -0.03340396469355156 1103 78-79 0.013368113988789787 1103 80-81 0.11312057149193677 1103 82-83 0.3426505320271751 1103 84-85 0.027210286895421376 1103 86-87 0.046762834629539896 1103 88-89 0.05364654123404122 1103 90-91 0.12409810199907767 1103 92-93 0.2579457605537563 1103 94-95 0.3514610824482034 1103 96-97 0.3283098442044867 1103 98-99 0.7904156270548484 1103 100-101 1.0297105491116305 1104 1 0.11342963595652478 1104 2 0.22006720223184928 1104 3 0.10525041676569558 1104 4 0.025897204308044763 1104 5 -0.060628068149846115 1104 6 0.07806775122710263 1104 7 0.08023819971035806 1104 8 -0.030272473202913375 1104 9 0.07585275726382434 1104 10-11 0.10887272226172229 1104 12-13 -0.010995701285168025 1104 14-15 0.061508035564664 1104 16-17 0.09299512488234285 1104 18-19 -0.09704735039414203 1104 20-21 0.004872950018189215 1104 22-23 0.039535759012686356 1104 24-25 0.019269916522603125 1104 26-27 0.22712805623618948 1104 28-29 0.1858623972938389 1104 30-31 0.05948292392746879 1104 32-33 0.1192804739684803 1104 34-35 0.1812374589339285 1104 36-37 0.438513506890871 1104 38-39 0.6159042416770077 1104 40-41 0.46916066014813396 1104 42-43 0.4768441127436276 1104 44-45 0.6162674610489631 1104 46-47 0.5960927173139865 1104 48-49 0.6178402966118028 1104 50-51 0.5386070583404887 1104 52-53 0.5959674341320422 1104 54-55 0.5980252968760382 1104 56-57 0.5747167945525931 1104 58-59 0.5185970093411143 1104 60-61 0.7443952406689647 1104 62-63 0.879767899810922 1104 64-65 0.7376718166066034 1104 66-67 0.46086534094631304 1104 68-69 0.5497444760607806 1104 70-71 0.3051757410292453 1104 72-73 0.501805775230018 1104 74-75 0.6133163251617617 1104 76-77 0.5092091247617283 1104 78-79 0.551217237388812 1104 80-81 0.9488707013254363 1104 82-83 0.6926175699878065 1104 84-85 0.5299079695913029 1104 86-87 0.6501412198141949 1104 88-89 0.9359818541551626 1104 90-91 0.8149116543779407 1104 92-93 0.7587884191409024 1104 94-95 0.8279344968006264 1104 96-97 1.001235054512371 1104 98-99 1.2089756056547074 1104 100-101 1.4305983416287482 1105 1 0.0577220584799214 1105 2 0.1449709298762194 1105 3 0.05240460770326294 1105 4 0.03570792816641699 1105 5 -0.054566759459405034 1105 6 -0.0157033226396166 1105 7 0.055352230901007715 1105 8 0.12565186825995767 1105 9 -0.046453991506481884 1105 10-11 0.057792176799985384 1105 12-13 -0.12243091605201784 1105 14-15 0.01586296210739846 1105 16-17 0.09128222329519531 1105 18-19 -0.045574606075931 1105 20-21 0.14720927521371863 1105 22-23 0.14351418055315435 1105 24-25 0.14432249978410283 1105 26-27 0.06816081804183227 1105 28-29 0.016318179859617032 1105 30-31 0.11346048997587843 1105 32-33 0.10307347203152517 1105 34-35 0.006575629042323783 1105 36-37 -0.07787049340176821 1105 38-39 0.11080713152916388 1105 40-41 0.08606114164265932 1105 42-43 0.07444941349038459 1105 44-45 0.12050952164022277 1105 46-47 -0.13907673796177988 1105 48-49 -0.28576684137789243 1105 50-51 -0.20091272152075845 1105 52-53 -0.1913870363449277 1105 54-55 -0.11419189662623808 1105 56-57 -0.14608467170179296 1105 58-59 -0.30336240965588246 1105 60-61 -0.2470663586527806 1105 62-63 -0.07018611011633169 1105 64-65 -0.2747938825870513 1105 66-67 0.0058581754628797 1105 68-69 0.015782326569151905 1105 70-71 -0.041304145172613715 1105 72-73 -0.09634143548239749 1105 74-75 -0.24896479424664264 1105 76-77 -0.283978031529589 1105 78-79 -0.014026273998965166 1105 80-81 0.06718097472299434 1105 82-83 0.11912682021687004 1105 84-85 -0.0033211117105338417 1105 86-87 0.004568922180840929 1105 88-89 0.007546124323091874 1105 90-91 0.1498741100767269 1105 92-93 -0.0194730558099927 1105 94-95 -0.1368519753330446 1105 96-97 0.06252707760947729 1105 98-99 0.2737106892657799 1105 100-101 0.22487698778012444 1106 1 0.06650061872876378 1106 2 0.05721434851187013 1106 3 0.05075765891169581 1106 4 0.06007412990148708 1106 5 -0.08750144807923022 1106 6 -0.0498371113407714 1106 7 -0.025642428552586694 1106 8 -0.022616395096193287 1106 9 0.024594667025041872 1106 10-11 0.10428170907839984 1106 12-13 0.06087474827778294 1106 14-15 0.07001720410531931 1106 16-17 -0.022264738489923275 1106 18-19 -0.06125277368216331 1106 20-21 0.023791503600733677 1106 22-23 0.03706057510720484 1106 24-25 -0.16046229209156593 1106 26-27 -0.1600514081619977 1106 28-29 -0.2590660697167735 1106 30-31 -0.10952084350519442 1106 32-33 -0.19497792437922357 1106 34-35 -0.2744997289233453 1106 36-37 -0.3109577737728699 1106 38-39 -0.05710002329919206 1106 40-41 0.06985531723497473 1106 42-43 0.05686310697586805 1106 44-45 -0.015409593848680458 1106 46-47 -0.030410106911944013 1106 48-49 -0.08454013825013362 1106 50-51 -0.20188987595997077 1106 52-53 -0.3765724780299493 1106 54-55 -0.45118090223720486 1106 56-57 -0.3138147317161568 1106 58-59 -0.10073395325648704 1106 60-61 -0.028308752974666618 1106 62-63 -0.0850053513854192 1106 64-65 -0.12249465105322699 1106 66-67 0.0015031930305546837 1106 68-69 0.0769281860792077 1106 70-71 0.14111472594523278 1106 72-73 0.0023991466681607676 1106 74-75 0.144506244320457 1106 76-77 -0.21076762903957658 1106 78-79 -0.06910754303995503 1106 80-81 -0.13731126443481045 1106 82-83 0.2223811765184429 1106 84-85 -0.03302621091990332 1106 86-87 0.01073558126244123 1106 88-89 0.12821167103819775 1106 90-91 0.1178296774166192 1106 92-93 0.11769198930136326 1106 94-95 0.44348311311872024 1106 96-97 0.41338339864114815 1106 98-99 0.9739429242155246 1106 100-101 0.8527519753307224 1107 1 0.06365438341297391 1107 2 0.0682773668597143 1107 3 0.060050453438581286 1107 4 -0.02912000264833381 1107 5 -0.023779378150109665 1107 6 -0.017918489508971902 1107 7 -0.05975393011166119 1107 8 -0.17486163132385713 1107 9 0.031035590824053827 1107 10-11 0.0037447247494313274 1107 12-13 0.029401500107269385 1107 14-15 0.14865198326232587 1107 16-17 0.16253151846937897 1107 18-19 0.10004011664673129 1107 20-21 0.1700019179127068 1107 22-23 0.011762505834610693 1107 24-25 -0.02526858008437216 1107 26-27 0.020028008882796655 1107 28-29 -0.05174278127066856 1107 30-31 -0.059927808310987984 1107 32-33 0.06907106989343248 1107 34-35 -0.05085875257410777 1107 36-37 0.0041330849195233554 1107 38-39 0.15318361755739573 1107 40-41 -0.08883700652367565 1107 42-43 -0.312586408361085 1107 44-45 -0.22739970792703446 1107 46-47 -0.1358992096099314 1107 48-49 -0.025766664557473007 1107 50-51 0.15057908612744342 1107 52-53 -0.052406844815678255 1107 54-55 0.008861032272967861 1107 56-57 -0.18269190051329787 1107 58-59 0.14843667258067939 1107 60-61 0.04890449348409476 1107 62-63 0.01533439352060384 1107 64-65 0.1519451625696071 1107 66-67 0.3304092644474288 1107 68-69 0.20726082684387492 1107 70-71 0.026853576018265102 1107 72-73 0.21889776652052007 1107 74-75 0.09772404700988702 1107 76-77 0.06590732990116166 1107 78-79 0.11091323049784307 1107 80-81 0.39904031342774005 1107 82-83 0.4424637423497444 1107 84-85 0.3730945158087522 1107 86-87 0.4945864274019556 1107 88-89 0.562693137400597 1107 90-91 0.47311247120033784 1107 92-93 0.4192111907352931 1107 94-95 0.8202949421304595 1107 96-97 1.0079355753366777 1107 98-99 1.2611911489726637 1107 100-101 1.386016051797629 1108 1 -0.034996402917865765 1108 2 0.11290147738858991 1108 3 0.06363120203205241 1108 4 -0.024920761115993173 1108 5 -0.04891282303952238 1108 6 0.06653021084204624 1108 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-1.0376475599630766 2316 88-89 -1.025477499686673 2316 90-91 -0.9969970752022341 2316 92-93 -0.7674812580797798 2316 94-95 -0.9776972113040792 2316 96-97 -0.6872980293797291 2316 98-99 -0.5539623415238886 2316 100-101 -0.6727008502307115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 29.0 5 60.0 6 147.0 7 312.0 8 762.0 9 1468.0 10 2514.0 11 3765.0 12 5373.0 13 7319.0 14 9512.0 15 13035.0 16 17037.0 17 21777.0 18 18327.0 19 11652.0 20 10211.0 21 10414.0 22 10823.0 23 12142.0 24 14126.0 25 16664.0 26 20752.0 27 25125.0 28 30763.0 29 37169.0 30 45028.0 31 55308.0 32 68405.0 33 85792.0 34 111645.0 35 148699.0 36 203242.0 37 251504.0 38 142972.0 39 7434.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58530776333866 19.76806547149839 15.51274183013896 21.133884935023985 2 16.065326007341472 21.2155433174247 39.148814310873384 23.570316364360444 3 19.430139854678348 25.678605126011337 30.572428287274473 24.31882673203585 4 13.011165755535387 17.53818660250051 36.65906804286186 32.791579599102235 5 13.061263545678539 38.66672951983463 33.25042812857507 15.021578805911762 6 31.054665650704667 36.792810591333506 16.783002758716766 15.369520999245065 7 28.087752415541363 34.58179983775611 19.77940178017993 17.5510459665226 8 27.70585309258948 33.574987142206844 18.951718722846486 19.767441042357195 9 27.373180261016582 16.49059410155148 20.220377417963554 35.91584821946838 10-11 25.41644621307637 27.065345258465417 25.88668075693444 21.631527771523775 12-13 26.949301486522238 24.756385685258046 27.024865897795262 21.269446930424454 14-15 23.401437221048987 25.615561135015547 26.675060482260943 24.307941161674528 16-17 21.482713032612313 29.070945553739502 27.30509902038525 22.141242393262942 18-19 21.465619562701356 26.48895355504789 31.131795370068748 20.91363151218201 20-21 22.572374776351793 25.186077419745207 31.36731636925471 20.874231434648287 22-23 22.106525536958248 25.380094041130153 31.402708660353635 21.11067176155796 24-25 21.987495963205543 25.66958019448379 31.414739031352262 20.92818481095841 26-27 21.50655885981697 25.83828761463097 31.474435627060913 21.180717898491146 28-29 21.15737032100001 26.229172537459615 31.568700502838915 21.044756638701465 30-31 21.450211637829135 25.69531610586394 31.466562911596174 21.387909344710742 32-33 21.241747061869596 25.932061363022335 31.234064019722624 21.592127555385446 34-35 21.95622735823533 25.818498583399972 31.028940999567617 21.19633305879708 36-37 22.227104840406405 25.452541303079705 31.004277548814652 21.316076307699237 38-39 21.797865865457347 25.83699518359679 30.99248162624852 21.372657324697347 40-41 21.80083782806372 25.886410552637244 30.97786242677447 21.33488919252456 42-43 21.81344035201341 26.033949678096153 30.381947070641544 21.770662899248897 44-45 21.84316850213097 26.16237570819723 30.213167147739483 21.78128864193231 46-47 22.37496011620213 26.581342016362996 29.352294158268055 21.691403709166817 48-49 22.49724372635532 26.80575500086892 28.75442463711116 21.942576635664597 50-51 22.3501566522503 27.11100604305492 28.632042051613276 21.90679525308151 52-53 22.71194883768545 27.177288367778214 28.466935274212236 21.643827520324102 54-55 22.023331875721404 27.20065687522009 28.55074793785437 22.225263311204134 56-57 22.524466252419888 27.37806978075269 28.04003674115393 22.0574272256735 58-59 22.70774357164894 26.786399648491187 28.279714050818917 22.226142729040955 60-61 22.338898571200406 26.979489369666275 28.906261543430034 21.775350515703288 62-63 22.34053406733819 27.036455386970854 28.878468440324113 21.744542105366847 64-65 22.001501571231753 27.15022326548821 28.65123158393952 22.19704357934052 66-67 22.165649148753687 27.2022396886163 28.349664982491003 22.28244618013902 68-69 22.3365882986594 26.980445933884933 28.58732487865559 22.095640888800077 70-71 22.299705132634028 27.21708105932737 28.232829129603036 22.250384678435566 72-73 22.354945143472676 27.258594869409908 28.105953816175845 22.28050617094157 74-75 22.264822434746385 27.838503993740606 27.807544764231395 22.08912880728161 76-77 22.386992233728332 27.45920209614754 27.920091410945393 22.233714259178736 78-79 22.379566624896306 27.50613362903277 27.82071298556284 22.293586760508084 80-81 22.47146778756224 27.851154393209615 27.51000971331815 22.167368105909997 82-83 22.48626730263579 27.77526432847866 27.560733808363523 22.177734560522023 84-85 22.272276797540716 27.802218717424704 27.361226377357866 22.564278107676714 86-87 22.36162943199536 27.65170384504973 27.407835041681444 22.578831681273464 88-89 22.199275670799626 27.599060497038725 27.292483624488717 22.909180207672936 90-91 22.22515414588666 27.543218020775612 27.573475472992587 22.658152360345145 92-93 22.35350280043214 27.496722187492633 27.622206032019314 22.527568980055914 94-95 21.90681873109763 27.33328713096296 27.676744294857432 23.083149843081973 96-97 22.125495234282493 27.158257563338275 27.66430943004875 23.051937772330483 98-99 21.787969317495808 27.476454090930197 27.31346560038726 23.42211099118673 100-101 21.6604426744814 27.421543397613718 27.44655663422451 23.471457293680377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 654.0 1 490.0 2 396.0 3 466.0 4 403.5 5 389.5 6 664.0 7 1409.5 8 2263.5 9 2798.5 10 2677.5 11 2278.0 12 2240.5 13 2391.0 14 2636.0 15 2859.5 16 2967.5 17 3139.5 18 3438.5 19 3712.5 20 4015.0 21 4435.0 22 4988.5 23 5632.0 24 6466.5 25 7685.5 26 9418.5 27 11481.0 28 13495.0 29 15730.0 30 18204.5 31 21102.0 32 24825.0 33 27346.5 34 29275.5 35 31280.0 36 34321.5 37 37747.5 38 38827.5 39 38815.0 40 38928.5 41 40689.0 42 42028.5 43 43141.0 44 45016.0 45 47109.0 46 47694.0 47 49392.0 48 57188.5 49 56938.0 50 49465.0 51 46101.5 52 44049.0 53 43948.0 54 44167.5 55 41713.0 56 38082.5 57 34978.0 58 33280.5 59 30419.0 60 25675.0 61 21763.0 62 18685.5 63 14852.5 64 10489.5 65 7799.0 66 6002.0 67 4758.5 68 3546.0 69 2303.5 70 1682.0 71 1286.0 72 1087.5 73 996.5 74 829.0 75 649.5 76 494.5 77 334.5 78 210.5 79 152.5 80 109.0 81 82.5 82 58.0 83 40.0 84 28.0 85 18.0 86 6.5 87 2.5 88 2.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0341232814354574 2 0.0053471533795768295 3 0.003940007753372401 4 4.925009691715501E-4 5 0.0010553592196533216 6 2.8142912524088573E-4 7 0.0 8 0.0 9 0.0019700038766862003 10-11 3.517864065511072E-4 12-13 9.146446570328787E-4 14-15 0.0022866116425821967 16-17 0.0030957203776497437 18-19 0.0 20-21 0.0 22-23 0.0016885747514453144 24-25 0.001125716500963543 26-27 0.005030545613680833 28-29 0.006085904833334154 30-31 0.0014775029075146501 32-33 3.517864065511072E-4 34-35 0.00932233977360434 36-37 0.012382881510598975 38-39 0.009287161132949228 40-41 0.0016182174701350929 42-43 5.276796098266608E-4 44-45 0.0010201805789982108 46-47 3.5178640655110716E-5 48-49 9.850019383431002E-4 50-51 0.001125716500963543 52-53 0.002568040767823082 54-55 0.002990184455684411 56-57 0.0015830388294799822 58-59 0.0010905378603084323 60-61 0.0037992931907519577 62-63 0.005136081535646164 64-65 0.009779662102120779 66-67 0.0033771495028906287 68-69 0.0036937572687866255 70-71 0.0 72-73 0.0013016097042390965 74-75 0.006543227161850593 76-77 0.0030253630963395215 78-79 0.0037992931907519577 80-81 0.005100902894991054 82-83 0.005206438816956386 84-85 0.00636733395857504 86-87 0.004291794159923507 88-89 0.009850019383431 90-91 0.012629131995184749 92-93 0.0013016097042390965 94-95 0.002356968923892418 96-97 0.0014071456262044289 98-99 0.0035882213468212934 100-101 0.004889831051060389 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1421317.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.102603153362224 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96954798775009 38.311390594049385 2 13.728415005372685 14.03115487888447 3 4.680801598841078 7.17603439635597 4 2.1173060886629993 4.327994112125713 5 1.1671283231346494 2.982164776309955 6 0.7251207248691877 2.223333398476107 7 0.4652334315557693 1.6642247598529967 8 0.34098926775517235 1.3940351383718517 9 0.253536791925953 1.1660751056311693 >10 1.3794576539471322 13.133867351549982 >50 0.1075479090994626 3.7478295478602224 >100 0.05762606830998869 5.223456396761919 >500 0.0055012443592559425 1.8765990634762006 >1k 0.0013753110898139856 1.044109047863467 >5k 4.1259332694419573E-4 1.6977314324306616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9859 0.6936524364374731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8831 0.6213251512505654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5466 0.38457289964167035 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2729 0.1920050206955943 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2309 0.1624549625453013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1478 0.10398806177650728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 7.035728131022143E-5 0.0 0.0 3.5178640655110716E-5 0.0 24-25 7.035728131022143E-5 0.0 0.0 7.035728131022143E-5 0.0 26-27 7.035728131022143E-5 0.0 0.0 2.46250484585775E-4 0.0 28-29 7.035728131022143E-5 0.0 0.0 8.091087350675464E-4 0.0 30-31 7.035728131022143E-5 0.0 0.0 0.0021107184393066433 0.0 32-33 7.035728131022143E-5 0.0 0.0 0.003588221346821293 0.0 34-35 7.035728131022143E-5 0.0 0.0 0.005276796098266607 0.0 36-37 1.4071456262044286E-4 0.0 0.0 0.007704122303469247 0.0 38-39 1.4071456262044286E-4 0.0 0.0 0.012699489276494969 0.0 40-41 1.4071456262044286E-4 0.0 0.0 0.018468786343933126 0.0 42-43 1.4071456262044286E-4 0.0 0.0 0.025856300881506377 0.0 44-45 2.110718439306643E-4 0.0 0.0 0.03239952804335697 0.0 46-47 2.110718439306643E-4 0.0 0.0 0.04559151828902349 0.0 48-49 2.110718439306643E-4 0.0 0.0 0.06391959007033618 0.0 50-51 2.110718439306643E-4 0.0 0.0 0.07176442693642587 0.0 52-53 2.110718439306643E-4 0.0 0.0 0.08629320552698659 0.0 54-55 2.110718439306643E-4 0.0 0.0 0.11165700543932142 0.0 56-57 2.110718439306643E-4 0.0 0.0 0.1503535101599432 0.0 58-59 2.110718439306643E-4 0.0 0.0 0.20129218182854353 0.0 60-61 2.110718439306643E-4 0.0 0.0 0.25979426123799265 0.0 62-63 2.110718439306643E-4 0.0 0.0 0.31819080472547645 0.0 64-65 2.110718439306643E-4 0.0 0.0 0.379366460824714 0.0 66-67 2.110718439306643E-4 0.0 0.0 0.46460430713204726 0.0 68-69 2.110718439306643E-4 0.0 0.0 0.5717936252081696 0.0 70-71 2.110718439306643E-4 0.0 0.0 0.6376128618738817 0.0 72-73 2.110718439306643E-4 0.0 0.0 0.7194383800376694 1.4071456262044286E-4 74-75 2.110718439306643E-4 0.0 0.0 0.8015453273266977 1.4071456262044286E-4 76-77 2.110718439306643E-4 0.0 0.0 0.8945928318594656 1.4071456262044286E-4 78-79 2.110718439306643E-4 0.0 0.0 1.0036114392496536 1.4071456262044286E-4 80-81 2.110718439306643E-4 0.0 0.0 1.1617042503537212 1.4071456262044286E-4 82-83 2.110718439306643E-4 0.0 0.0 1.326199574057019 1.4071456262044286E-4 84-85 2.110718439306643E-4 0.0 0.0 1.5677361207950091 1.4071456262044286E-4 86-87 2.110718439306643E-4 0.0 0.0 1.8810722731100804 1.4071456262044286E-4 88-89 2.110718439306643E-4 0.0 0.0 2.2455933475783376 1.4071456262044286E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9250 0.0 33.182755 1 GTATCAA 13460 0.0 29.263828 1 GTAAGGT 660 0.0 28.784212 4 TAAGGTG 730 0.0 28.626488 5 GGTAAGG 640 0.0 28.200523 3 GTACATG 9765 0.0 25.447851 1 TGCACCG 345 0.0 24.779451 5 GTATAGG 500 0.0 24.707285 1 TACATGG 9905 0.0 24.408857 2 TATCAAC 16615 0.0 23.61369 2 AGGTAAG 665 0.0 23.570913 2 ATCAACG 16705 0.0 23.484814 3 TCAACGC 16795 0.0 23.358143 4 GAGTACT 9785 0.0 23.279057 12-13 CAACGCA 16925 0.0 23.150671 5 ACATGGG 9910 0.0 23.100796 3 AACGCAG 17295 0.0 22.65779 6 GTACTTT 10315 0.0 22.196909 14-15 AGTACTT 9950 0.0 21.62782 12-13 TACCTGG 1110 0.0 21.39593 2 >>END_MODULE