FastQCFastQC Report
Thu 26 May 2016
SRR936716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461174
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81110.5551015826999385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66710.4565506914303156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.2713571415861492No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22490.15391732948984857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.12154609923253493No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15410.10546314128228398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79650.032.755341
GTATCAA118950.030.4828531
GTACATG101750.027.50911
TACATGG103250.026.7412412
ACATGGG103650.025.4018733
ATCAACG147000.024.5385953
TATCAAC147000.024.5369152
GTATAGG3700.024.4032341
TCAACGC148250.024.395814
CAACGCA149950.023.9924535
GAGTACT74950.023.58587312-13
AACGCAG153850.023.476936
CATGGGA62450.022.2216194
GTACTTT79250.022.15546814-15
AGTACTT76900.021.62829812-13
TAAGGTG7550.021.4021425
CATGGGG38800.021.1904244
ACGCAGA169600.021.1566247
CGCAGAG169900.021.037518
GCAGAGT174500.020.0192099
Produced by FastQC (version 0.11.5)