##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936716_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1461174 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.356081479686882 31.0 31.0 34.0 30.0 34.0 2 31.394938590475878 31.0 31.0 34.0 28.0 34.0 3 31.345741848677843 31.0 31.0 34.0 28.0 34.0 4 35.036749901106916 37.0 35.0 37.0 32.0 37.0 5 35.07514231706833 37.0 35.0 37.0 32.0 37.0 6 35.13805063599544 37.0 35.0 37.0 32.0 37.0 7 35.02135885253912 37.0 35.0 37.0 32.0 37.0 8 34.998214449476926 37.0 35.0 37.0 32.0 37.0 9 36.46821323127841 39.0 37.0 39.0 32.0 39.0 10-11 36.346136394433515 39.0 37.0 39.0 32.0 39.0 12-13 36.24668040904095 39.0 35.0 39.0 32.0 39.0 14-15 37.195065064119675 39.0 36.5 41.0 31.0 41.0 16-17 37.125454600205046 39.0 36.0 41.0 31.0 41.0 18-19 37.05739597063731 39.0 36.0 41.0 31.0 41.0 20-21 37.063953711193875 39.0 36.0 41.0 31.0 41.0 22-23 37.030227063991006 39.0 36.0 41.0 31.0 41.0 24-25 37.11813685433768 39.0 36.0 41.0 31.0 41.0 26-27 37.02450529505727 39.0 36.0 41.0 31.0 41.0 28-29 36.898083322041046 39.0 36.0 41.0 30.5 41.0 30-31 36.65742512527598 39.0 36.0 41.0 30.0 41.0 32-33 36.44706243062086 39.0 35.0 40.5 30.0 41.0 34-35 36.23140673184713 39.0 35.0 40.0 30.0 41.0 36-37 35.98859376090732 38.0 35.0 40.0 29.5 41.0 38-39 35.77449229181467 38.0 35.0 40.0 28.5 41.0 40-41 35.51737541182638 38.0 35.0 40.0 27.5 41.0 42-43 35.190900604582346 38.0 34.0 40.0 26.0 41.0 44-45 34.96334043721008 38.0 34.0 40.0 25.5 41.0 46-47 34.70000321659159 38.0 34.0 40.0 24.0 41.0 48-49 34.394235730994396 38.0 34.0 40.0 23.0 41.0 50-51 33.3716319890718 37.0 32.0 39.5 21.5 40.5 52-53 33.45952946055706 37.5 33.0 39.5 21.5 40.5 54-55 33.88390978760914 38.0 33.0 40.0 21.5 41.0 56-57 33.73763528505161 38.0 33.0 40.0 20.0 41.0 58-59 33.521742448195766 37.5 33.0 40.0 19.5 41.0 60-61 33.232860699683954 37.0 33.0 40.0 18.5 41.0 62-63 32.89008564346204 36.5 32.0 40.0 17.5 41.0 64-65 32.5202655535891 36.0 32.0 39.0 13.5 41.0 66-67 32.16341003877704 35.5 31.0 39.0 12.5 41.0 68-69 31.730983442081502 35.0 31.0 39.0 8.0 40.0 70-71 31.334145351614524 35.0 31.0 38.0 7.5 40.0 72-73 30.92420409889582 35.0 30.5 37.0 6.5 39.5 74-75 30.520579000173832 35.0 30.0 37.0 2.0 39.0 76-77 30.07087862225854 34.5 29.5 36.0 2.0 39.0 78-79 29.626717967880623 34.0 29.0 36.0 2.0 38.5 80-81 29.185987089833244 34.0 29.0 35.0 2.0 37.0 82-83 28.793293269658506 34.0 29.0 35.0 2.0 37.0 84-85 28.43842280248622 34.0 28.0 35.0 2.0 36.0 86-87 28.093953218439417 34.0 27.0 35.0 2.0 36.0 88-89 27.746476805637112 34.0 27.0 35.0 2.0 36.0 90-91 27.388207017097212 33.5 26.0 35.0 2.0 35.0 92-93 26.97791775654371 33.0 25.5 35.0 2.0 35.0 94-95 26.487840599408422 33.0 25.0 35.0 2.0 35.0 96-97 25.90852218832254 33.0 23.5 35.0 2.0 35.0 98-99 25.183536662984693 32.5 19.0 35.0 2.0 35.0 100-101 22.700803942583157 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36969708352299335 1101 2 0.3393175878682513 1101 3 0.4754888699236304 1101 4 0.49029681046862805 1101 5 0.3729602455844798 1101 6 0.3761956354786804 1101 7 0.4459920377261639 1101 8 0.41496195189785823 1101 9 0.5816504979132873 1101 10-11 0.6522573412516905 1101 12-13 0.6718409759697934 1101 14-15 0.8367019768330906 1101 16-17 0.9015609295399116 1101 18-19 0.9595348296135384 1101 20-21 0.805671603260727 1101 22-23 0.7366626186144316 1101 24-25 0.46690480568865667 1101 26-27 0.4764507393037434 1101 28-29 0.520085465163767 1101 30-31 0.38693946359185816 1101 32-33 0.2951306054821785 1101 34-35 0.3515781230897659 1101 36-37 0.40496578305094744 1101 38-39 0.35118161538395043 1101 40-41 0.313099521112413 1101 42-43 0.4872926861569695 1101 44-45 0.4156971301718855 1101 46-47 0.594862433310972 1101 48-49 0.5718860401492876 1101 50-51 0.48767018514863025 1101 52-53 0.37437143256120464 1101 54-55 0.372340715196529 1101 56-57 0.48820800790585395 1101 58-59 0.5867334383256448 1101 60-61 0.4545310744880169 1101 62-63 0.5838312434268573 1101 64-65 0.3310825838772189 1101 66-67 0.28262439689098073 1101 68-69 0.5048324091119198 1101 70-71 0.31202035692300356 1101 72-73 0.3549832551410965 1101 74-75 0.17049175703489894 1101 76-77 0.1691684318078721 1101 78-79 0.14753871844665056 1101 80-81 0.1828176074081398 1101 82-83 0.16435891900056632 1101 84-85 0.10737902902319973 1101 86-87 0.14262250372039986 1101 88-89 0.36186239040222645 1101 90-91 0.4003570142461079 1101 92-93 0.47181940655329413 1101 94-95 0.5090836209934899 1101 96-97 0.2757650925704702 1101 98-99 0.20752401651283847 1101 100-101 0.9393633378085333 1102 1 0.3112112112122709 1102 2 0.39824265384152113 1102 3 0.449247048586507 1102 4 0.31742586330528866 1102 5 0.3945937997792939 1102 6 0.3418314951889698 1102 7 0.34629809138769474 1102 8 0.3517705767769783 1102 9 0.4318980217328843 1102 10-11 0.44817701102227403 1102 12-13 0.30431088002306694 1102 14-15 0.5576332231757632 1102 16-17 0.3982061733715341 1102 18-19 0.47146052045788167 1102 20-21 0.4576175352042995 1102 22-23 0.31167151932123716 1102 24-25 0.5546382623714194 1102 26-27 0.4998528771316586 1102 28-29 0.11605078795980006 1102 30-31 0.11711236971471095 1102 32-33 0.3563869405618192 1102 34-35 0.1021786852475941 1102 36-37 0.19105497705269414 1102 38-39 0.17010493262272774 1102 40-41 0.301552848368722 1102 42-43 0.03208918346188483 1102 44-45 0.29301850611437175 1102 46-47 0.29259342518262343 1102 48-49 0.441079695157633 1102 50-51 0.4141244676762881 1102 52-53 0.25336202080181636 1102 54-55 0.48375809214001464 1102 56-57 0.2385671140512784 1102 58-59 0.26335964205672724 1102 60-61 0.29613191026250263 1102 62-63 0.4492828327526439 1102 64-65 0.33478922261102184 1102 66-67 0.21903176760585552 1102 68-69 0.4044795466521478 1102 70-71 0.16584738446883662 1102 72-73 0.4024830045142771 1102 74-75 0.223876902490165 1102 76-77 0.2718191464649031 1102 78-79 0.05276999737708721 1102 80-81 0.21620574782247104 1102 82-83 0.15830028262653784 1102 84-85 0.2718600707061434 1102 86-87 -0.04903439959744915 1102 88-89 0.1197389191944147 1102 90-91 0.2436510571669892 1102 92-93 0.2259173120668585 1102 94-95 0.570851511162914 1102 96-97 0.47411655855519186 1102 98-99 0.3816748564204673 1102 100-101 0.8832294631380257 1103 1 0.385786994599286 1103 2 0.453693789267124 1103 3 0.4355569963067545 1103 4 0.36266155718548276 1103 5 0.4710579970503872 1103 6 0.319197083214803 1103 7 0.5363435428063639 1103 8 0.31740331555624834 1103 9 0.3354386902824871 1103 10-11 0.33502986192786466 1103 12-13 0.3143766040086291 1103 14-15 0.6974766317590309 1103 16-17 0.7509915775280191 1103 18-19 0.7940210599671289 1103 20-21 0.6308690768905265 1103 22-23 0.6028931640293251 1103 24-25 0.4913612231777975 1103 26-27 0.5776762923510574 1103 28-29 0.3681658013939071 1103 30-31 0.39381318142667254 1103 32-33 0.4122664907617164 1103 34-35 0.3167129473018875 1103 36-37 0.46737567813551806 1103 38-39 0.295169043098106 1103 40-41 0.32101752210584067 1103 42-43 0.5048875007579028 1103 44-45 0.4596764055365483 1103 46-47 0.5693473461641219 1103 48-49 0.7151494483664464 1103 50-51 0.5302614157445262 1103 52-53 0.37169592232004334 1103 54-55 0.29159636278081535 1103 56-57 0.44083579030525755 1103 58-59 0.4562153325379299 1103 60-61 0.47545295630267503 1103 62-63 0.40962323282224133 1103 64-65 0.49004620572127067 1103 66-67 0.39129105110617957 1103 68-69 0.4988416942089202 1103 70-71 0.3992563015657744 1103 72-73 0.5672653880178302 1103 74-75 0.36695795330511416 1103 76-77 0.3843369507152623 1103 78-79 0.2390378623237588 1103 80-81 0.32666253306275195 1103 82-83 0.21961683107028662 1103 84-85 0.4777846879147596 1103 86-87 0.3430838560311251 1103 88-89 0.2561823229219762 1103 90-91 0.40727553214392387 1103 92-93 0.20322346829106408 1103 94-95 0.44366581182005405 1103 96-97 0.5265322463252922 1103 98-99 0.5779970647949391 1103 100-101 0.7409796988420183 1104 1 0.12827061030828446 1104 2 0.16479795973846123 1104 3 0.29558700477387134 1104 4 0.3409508151503857 1104 5 0.24123692720495882 1104 6 0.12576620852816944 1104 7 0.1951863386989814 1104 8 0.11455251117991594 1104 9 0.20225510528754853 1104 10-11 0.2485603134660579 1104 12-13 0.3721338238880989 1104 14-15 0.43050828073052827 1104 16-17 0.31290349665872697 1104 18-19 0.35048525422111254 1104 20-21 0.41384518633218903 1104 22-23 0.3587171620868759 1104 24-25 0.33206822245559664 1104 26-27 0.06118888615345952 1104 28-29 -0.09803342701908235 1104 30-31 -0.11027251495238488 1104 32-33 -0.10482702525110454 1104 34-35 0.00844041086006797 1104 36-37 0.03176599366022259 1104 38-39 0.0030299488723457557 1104 40-41 -0.02126435099528834 1104 42-43 0.17785993177578518 1104 44-45 0.13547082055821136 1104 46-47 0.33665409661653456 1104 48-49 0.3477943699625783 1104 50-51 0.23469319006259326 1104 52-53 0.2648404350871232 1104 54-55 0.3599836567060706 1104 56-57 0.34759397311202633 1104 58-59 0.2083064308561262 1104 60-61 0.11160327568739348 1104 62-63 0.2702222555755469 1104 64-65 0.17023799738862522 1104 66-67 0.03944812965945488 1104 68-69 0.06000794132428666 1104 70-71 0.11381813851561873 1104 72-73 0.23429591945408745 1104 74-75 0.05667499483550387 1104 76-77 0.1359048204876494 1104 78-79 0.02785275615272198 1104 80-81 0.20061205512706692 1104 82-83 0.15727675689997866 1104 84-85 0.049707730515994086 1104 86-87 -0.09920704451343099 1104 88-89 0.03597301593909208 1104 90-91 0.304241662628872 1104 92-93 0.20580355312828047 1104 94-95 0.2204299766051392 1104 96-97 0.08958154550514408 1104 98-99 0.07376973094140382 1104 100-101 0.7968980959416143 1105 1 0.3065361923153809 1105 2 0.3237019162988837 1105 3 0.49401640282505 1105 4 0.4917216868012204 1105 5 0.44856383184242077 1105 6 0.32370993017654115 1105 7 0.48800984479853327 1105 8 0.3268087919253446 1105 9 0.3025401010518465 1105 10-11 0.3965221881344192 1105 12-13 0.3654813072677783 1105 14-15 0.5823854891817248 1105 16-17 0.6363756839712948 1105 18-19 0.4689159555660254 1105 20-21 0.46166010798163626 1105 22-23 0.36464063214636155 1105 24-25 0.16675910910760905 1105 26-27 0.201736083340478 1105 28-29 0.28469154741375036 1105 30-31 0.23830190777493243 1105 32-33 0.4406345202690929 1105 34-35 0.21446043104782575 1105 36-37 0.461670866515945 1105 38-39 0.18310391744169152 1105 40-41 0.13961422857412487 1105 42-43 -0.15775589446590743 1105 44-45 0.11312518439792285 1105 46-47 0.2984577487647684 1105 48-49 0.32495880174645464 1105 50-51 0.5431288253052529 1105 52-53 0.6192488261710025 1105 54-55 0.40934324741864003 1105 56-57 0.2730753606145129 1105 58-59 0.3659315636790481 1105 60-61 0.23582937853322505 1105 62-63 0.3700574820095355 1105 64-65 0.312867405812959 1105 66-67 0.4128715764458377 1105 68-69 0.4041603415791002 1105 70-71 0.5403137013323835 1105 72-73 0.4463726648370354 1105 74-75 0.3475704241311526 1105 76-77 0.35471600381186974 1105 78-79 0.3918291851544673 1105 80-81 0.40021282167656835 1105 82-83 0.29412100398851493 1105 84-85 0.38674135416465916 1105 86-87 0.537547966850493 1105 88-89 0.5487837284816983 1105 90-91 0.43659366896979535 1105 92-93 0.7121116278309501 1105 94-95 0.7534519823210104 1105 96-97 0.8377156411323305 1105 98-99 1.0384184201337732 1105 100-101 1.489978537093247 1106 1 0.07834226153259749 1106 2 0.019391337306426948 1106 3 0.01880519211581344 1106 4 0.23645378177464949 1106 5 0.3727480674790442 1106 6 0.15230643240182928 1106 7 0.25866252593827 1106 8 0.28914816383031194 1106 9 0.3853096254185644 1106 10-11 0.3784085626930178 1106 12-13 0.4251802841233996 1106 14-15 0.2020481092392501 1106 16-17 0.21053449770887056 1106 18-19 0.29026826129744876 1106 20-21 0.07866492073019771 1106 22-23 -0.10188603995293022 1106 24-25 -0.16987489216000284 1106 26-27 -0.13393397523437045 1106 28-29 -0.007035539793811552 1106 30-31 -0.17905602559880407 1106 32-33 -0.1025903695113044 1106 34-35 -0.05078539523354664 1106 36-37 -0.29921771591717317 1106 38-39 -0.1552587729613677 1106 40-41 -0.10546889755332245 1106 42-43 -0.17204996663623717 1106 44-45 -0.35812856794402137 1106 46-47 -0.23262313466363338 1106 48-49 -0.14280135482457723 1106 50-51 -0.17228659638485766 1106 52-53 -0.1267592391876633 1106 54-55 0.12931998463978545 1106 56-57 0.30008525774287875 1106 58-59 0.1840751382069996 1106 60-61 0.2928176148106303 1106 62-63 0.2847736273531467 1106 64-65 -0.04600622993649495 1106 66-67 0.002422827690821805 1106 68-69 -0.011806700495888833 1106 70-71 -0.19752522830119545 1106 72-73 -0.06244072449412741 1106 74-75 0.03657214446240076 1106 76-77 0.016711309466675317 1106 78-79 0.06733431480614982 1106 80-81 0.14077502871530356 1106 82-83 0.15713109613419007 1106 84-85 0.3695248083390723 1106 86-87 0.5079791640478142 1106 88-89 0.6064183078363321 1106 90-91 0.6059195359262013 1106 92-93 0.6824975531532473 1106 94-95 0.7047283009514338 1106 96-97 0.6774706158149009 1106 98-99 0.6576227495180085 1106 100-101 1.0115704355170898 1107 1 0.19626351339470816 1107 2 0.15669979299658365 1107 3 0.24996311505686108 1107 4 0.18537481375124543 1107 5 0.14698622242115533 1107 6 0.14885787032203268 1107 7 0.3227147392825529 1107 8 0.37588848486316806 1107 9 0.3133258066624691 1107 10-11 0.47682698880783647 1107 12-13 0.28554002264893796 1107 14-15 0.1708635379213561 1107 16-17 0.2043485794101727 1107 18-19 0.46022549283689784 1107 20-21 0.0813230599162651 1107 22-23 0.21720555619747017 1107 24-25 0.13607089950793494 1107 26-27 0.15061433477799824 1107 28-29 0.22346170726867598 1107 30-31 0.2677136232105468 1107 32-33 0.19616327279758394 1107 34-35 0.16468595339274827 1107 36-37 0.3198309224672684 1107 38-39 0.31291432790492024 1107 40-41 0.3974015549302905 1107 42-43 0.3958279634110511 1107 44-45 0.3698056294956942 1107 46-47 0.577894382639613 1107 48-49 0.5486866002547046 1107 50-51 0.5409946342750516 1107 52-53 0.41393428846039626 1107 54-55 0.5591323829791364 1107 56-57 0.7618427982869989 1107 58-59 0.701852226718934 1107 60-61 0.6454181614664023 1107 62-63 0.6573553599636313 1107 64-65 0.6978505911014423 1107 66-67 0.7453396745616772 1107 68-69 0.7153300843119155 1107 70-71 0.5256725477971038 1107 72-73 0.46267937522614133 1107 74-75 0.34605940348025754 1107 76-77 0.44916568322254236 1107 78-79 0.4200113117233819 1107 80-81 0.4375737286239989 1107 82-83 0.4450274907398608 1107 84-85 0.4812802373997833 1107 86-87 0.6876059702762554 1107 88-89 0.64851779219752 1107 90-91 0.604032597853827 1107 92-93 0.7134320957112905 1107 94-95 0.7748990107447824 1107 96-97 1.074240628673106 1107 98-99 0.9998069844547537 1107 100-101 1.6479367327469703 1108 1 0.11097117299352632 1108 2 0.11949054029509654 1108 3 0.23374984007424615 1108 4 0.3327016505234468 1108 5 0.2568186483891637 1108 6 0.2404983732478172 1108 7 0.25973359560457254 1108 8 0.25778453406555 1108 9 -0.10908548637433313 1108 10-11 0.17041054352667828 1108 12-13 0.061440186275383724 1108 14-15 0.5077084865977426 1108 16-17 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-3.766364949613699 2316 68-69 -4.137447959571382 2316 70-71 -3.544117589441658 2316 72-73 -2.479438763894695 2316 74-75 -2.4678608261221022 2316 76-77 -3.340844476577626 2316 78-79 -3.3213706166053854 2316 80-81 -4.039825766579259 2316 82-83 -3.137040103538066 2316 84-85 -3.3585689882983196 2316 86-87 -3.9422807860755746 2316 88-89 -5.1824160296403825 2316 90-91 -4.182238833687432 2316 92-93 -3.9222690119610846 2316 94-95 -3.39056138330427 2316 96-97 -4.8878950538614845 2316 98-99 -6.999199518729711 2316 100-101 -7.59374301653601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3268.0 3 1264.0 4 1378.0 5 1512.0 6 1684.0 7 1969.0 8 2604.0 9 3278.0 10 4238.0 11 5429.0 12 7039.0 13 8451.0 14 9866.0 15 12442.0 16 15680.0 17 20125.0 18 15451.0 19 10565.0 20 10468.0 21 10996.0 22 12210.0 23 13683.0 24 15354.0 25 17622.0 26 20698.0 27 24206.0 28 29253.0 29 34445.0 30 41242.0 31 51292.0 32 63597.0 33 80785.0 34 106264.0 35 142572.0 36 194777.0 37 247345.0 38 190008.0 39 28107.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.930354180735314 18.768483297742215 14.7896516818688 21.51151083965367 2 15.607465886779432 20.30410946900304 40.26647059951093 23.821954044706597 3 19.30498362702472 25.617888159984233 29.97240140621407 25.104726806776988 4 12.536366113148754 16.619638864815947 37.36766157506855 33.47633344696674 5 13.184086243213475 38.35051913370696 33.38857707323184 15.076817549847721 6 30.967191162617482 36.00257136284969 17.65409008259031 15.376147391942515 7 28.29394952031906 34.11648914784803 20.02441040716203 17.56515092467088 8 27.43016870717182 33.23573055882154 19.524001585930957 19.81009914807569 9 27.546428353741014 16.54764945865262 20.460184651882372 35.445737535724 10-11 25.180015243516195 26.73918864227932 26.359949681331074 21.720846432873405 12-13 26.774774959818835 24.51453111707909 27.127752987270405 21.58294093583167 14-15 23.417433359669506 25.278823389260047 26.61501901270846 24.68872423836199 16-17 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26.124667589937644 30.179702479519843 21.718700754460908 42-43 21.817272192509893 26.270711132844514 29.661521256853806 22.25049541779179 44-45 21.855132471576567 26.35565859375668 29.584902432138794 22.204306502527952 46-47 22.358099433054743 26.63985699072794 28.912052308981057 22.089991267236257 48-49 22.38493194092079 26.911995083348334 28.418267167730775 22.2848058080001 50-51 22.212268128161888 27.211987527649416 28.373160355664574 22.202583988524125 52-53 22.60416474184062 27.229804040731715 28.08716433600174 22.078866881425924 54-55 21.904888352939807 27.152166727006566 28.363321920081685 22.579622999971942 56-57 22.32864753953906 27.390728404301225 27.813076078360517 22.4675479777992 58-59 22.54573079443514 26.880861249749767 27.98982980667393 22.58357814914116 60-61 22.204149822890106 27.039039462431862 28.57319338839921 22.183617326278824 62-63 22.230990106700897 27.08668331348012 28.57196142328544 22.110365156533543 64-65 21.918150411177905 27.2062486996704 28.400122367859137 22.475478521292555 66-67 21.96991991327474 27.291526082594313 28.105259771990315 22.63329423214063 68-69 22.088811748304206 27.080691226482823 28.42377694264578 22.406720082567194 70-71 22.127850915540456 27.26510242233502 28.05767311155772 22.549373550566802 72-73 22.158459677634646 27.333561961244147 27.955375117479836 22.55260324364137 74-75 22.01630175495757 27.872400573052957 27.757748912178197 22.35354875981127 76-77 22.198959438215745 27.429183989682688 27.797053144908208 22.574803427193363 78-79 22.19116457726332 27.581322106490948 27.72051678978112 22.506996526464615 80-81 22.304312367834125 27.82979139908371 27.49690194117641 22.368994291905757 82-83 22.30129439040457 27.7294527286571 27.552087878243466 22.417165002694865 84-85 22.11619306583918 27.786981090301094 27.341332401633395 22.755493442226335 86-87 22.32899109743545 27.663309498258908 27.394651617353976 22.613047786951665 88-89 22.19935208502079 27.54778176208647 27.277035838588613 22.975830314304126 90-91 22.043454157198784 27.544213869192653 27.615907410956282 22.796424562652284 92-93 22.2763774124717 27.55324591768464 27.61019214217392 22.560184527669737 94-95 21.97947553299548 27.313776704531456 27.68195221315986 23.024795549313204 96-97 21.982086994196806 27.15735840830168 27.722893582536134 23.137661014965378 98-99 21.82212040840984 27.494144621934076 27.334133559778934 23.34960140987715 100-101 21.930491358907794 27.352090067920575 27.499259872783682 23.218158700387946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 418.0 1 323.5 2 285.0 3 376.5 4 360.5 5 368.0 6 620.0 7 1190.0 8 1958.5 9 2384.0 10 2197.0 11 1943.0 12 2034.5 13 2183.0 14 2318.0 15 2528.0 16 2665.0 17 2839.0 18 3001.5 19 3212.0 20 3565.5 21 3946.0 22 4504.5 23 5349.5 24 6290.0 25 7439.5 26 9232.5 27 11412.5 28 13257.5 29 15297.5 30 18000.5 31 21266.5 32 25376.0 33 28189.0 34 30350.5 35 32531.5 36 35510.5 37 38847.0 38 39924.5 39 40105.5 40 40564.5 41 42382.0 42 43721.5 43 44679.5 44 46467.0 45 48752.5 46 49874.0 47 51928.5 48 58757.0 49 58703.5 50 51818.0 51 48050.5 52 46114.5 53 45925.0 54 46103.0 55 43857.5 56 40075.5 57 36512.5 58 34255.0 59 30979.0 60 26360.5 61 22573.0 62 19343.5 63 15155.5 64 10890.5 65 8383.5 66 6296.0 67 4943.5 68 3702.0 69 2433.0 70 1801.0 71 1415.0 72 1138.5 73 994.0 74 789.0 75 626.0 76 473.0 77 294.5 78 194.5 79 140.0 80 100.0 81 66.5 82 45.5 83 28.0 84 17.5 85 14.5 86 10.0 87 7.0 88 6.0 89 7.0 90 5.0 91 2.0 92 3.0 93 1.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07336566350071928 2 0.030797153521757163 3 0.015740767355564775 4 0.022995207962912014 5 0.025664294601464306 6 0.032165915900501926 7 0.07808789370738872 8 0.057008953074719364 9 0.06987531943492015 10-11 0.060293982783706804 12-13 0.04116552854074874 14-15 0.03914660403210021 16-17 0.04917278845640561 18-19 0.04585353968794955 20-21 0.01834141587517982 22-23 0.014098252501071058 24-25 0.021558007465230015 26-27 0.024124436925376445 28-29 0.005919897288071099 30-31 0.0012318861408702865 32-33 0.009102269818652673 34-35 0.011292289624644293 36-37 0.0040378490172970505 38-39 0.008588983926623387 40-41 0.00441425867145186 42-43 0.0022926769843974776 44-45 7.870383677782386E-4 46-47 6.843811893723813E-4 48-49 0.0011634480219330483 50-51 0.001505638616619239 52-53 2.053143568117144E-4 54-55 4.7906683256066694E-4 56-57 0.0037983156010167165 58-59 0.0028401819358953827 60-61 0.005167077979761479 62-63 0.0022926769843974776 64-65 4.106287136234288E-4 66-67 5.475049514979052E-4 68-69 0.004174725255171526 70-71 0.002908620054832621 72-73 0.019504863897112867 74-75 0.015535452998753058 76-77 0.022481922070882728 78-79 0.018512511172522915 80-81 0.012216204230297008 82-83 0.004208944314640146 84-85 0.0031139344116443354 86-87 0.001505638616619239 88-89 0.0017451720328995723 90-91 0.006433183180100385 92-93 0.010026184424305388 94-95 0.012729490122326294 96-97 0.011531823040924625 98-99 0.023097865141317873 100-101 0.018923139886146346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1461174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46002337896804 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41452704126772 40.85122402118958 2 12.971197605646742 13.86881054502178 3 4.444494892154046 7.128084025267779 4 2.0247487588441837 4.329724639373864 5 1.109759256069998 2.966387788726414 6 0.6518442952372414 2.090856675769792 7 0.4427916014039346 1.6570154554145529 8 0.30137411476163045 1.288917377677805 9 0.21818154115447255 1.0497591261879642 >10 1.2619935795783073 12.534173714193836 >50 0.10172248615637974 3.7228656329243606 >100 0.05184658946763958 5.007725678108647 >500 0.004106595912743781 1.476672271701283 >1k 0.0011549801004591882 1.0136514037533197 >5k 2.566622445464863E-4 1.0141316446891082 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8111 0.5551015826999385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6671 0.4565506914303156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3965 0.2713571415861492 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2249 0.15391732948984857 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1776 0.12154609923253493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1541 0.10546314128228398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.843811893723813E-5 0.0 22-23 0.0 0.0 0.0 6.843811893723813E-5 0.0 24-25 0.0 0.0 0.0 1.026571784058572E-4 0.0 26-27 0.0 0.0 0.0 2.053143568117144E-4 0.0 28-29 0.0 0.0 0.0 7.186002488410004E-4 0.0 30-31 0.0 0.0 0.0 0.0018478292113054298 0.0 32-33 0.0 0.0 0.0 0.0034561250063305264 0.0 34-35 0.0 0.0 0.0 0.005543487633916289 0.0 36-37 0.0 0.0 0.0 0.009068050759184054 0.0 38-39 0.0 0.0 0.0 0.01392715720372796 0.0 40-41 0.0 0.0 0.0 0.018512511172522915 0.0 42-43 0.0 0.0 0.0 0.025869608958276016 0.0 44-45 0.0 0.0 0.0 0.03202903966262745 0.0 46-47 0.0 0.0 0.0 0.04458743448761064 0.0 48-49 0.0 0.0 0.0 0.06104680209201642 0.0 50-51 0.0 0.0 0.0 0.06922515730501638 0.0 52-53 0.0 0.0 0.0 0.0832549716871502 0.0 54-55 0.0 0.0 0.0 0.10785847544508731 0.0 56-57 0.0 0.0 0.0 0.14652601264462684 0.0 58-59 0.0 0.0 0.0 0.19552770580368936 0.0 60-61 0.0 0.0 0.0 0.2538712021976849 0.0 62-63 0.0 0.0 0.0 0.31057218373718665 0.0 64-65 0.0 0.0 0.0 0.37254290043485583 0.0 66-67 0.0 0.0 0.0 0.454086919148575 0.0 68-69 0.0 0.0 0.0 0.5574626978032733 0.0 70-71 0.0 0.0 0.0 0.6224789107936495 0.0 72-73 0.0 0.0 0.0 0.7047073106967411 0.0 74-75 0.0 0.0 0.0 0.7909393405576612 0.0 76-77 0.0 0.0 0.0 0.8844942491448657 0.0 78-79 0.0 0.0 0.0 1.00059951792189 0.0 80-81 0.0 0.0 0.0 1.1621819167327097 0.0 82-83 0.0 0.0 0.0 1.33091609897247 0.0 84-85 3.421905946861907E-5 0.0 0.0 1.5678146476737198 0.0 86-87 6.843811893723813E-5 0.0 0.0 1.8780446408162204 0.0 88-89 6.843811893723813E-5 0.0 0.0 2.2292690672021265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7965 0.0 32.75534 1 GTATCAA 11895 0.0 30.482853 1 GTACATG 10175 0.0 27.5091 1 TACATGG 10325 0.0 26.741241 2 ACATGGG 10365 0.0 25.401873 3 ATCAACG 14700 0.0 24.538595 3 TATCAAC 14700 0.0 24.536915 2 GTATAGG 370 0.0 24.403234 1 TCAACGC 14825 0.0 24.39581 4 CAACGCA 14995 0.0 23.992453 5 GAGTACT 7495 0.0 23.585873 12-13 AACGCAG 15385 0.0 23.47693 6 CATGGGA 6245 0.0 22.221619 4 GTACTTT 7925 0.0 22.155468 14-15 AGTACTT 7690 0.0 21.628298 12-13 TAAGGTG 755 0.0 21.402142 5 CATGGGG 3880 0.0 21.190424 4 ACGCAGA 16960 0.0 21.156624 7 CGCAGAG 16990 0.0 21.03751 8 GCAGAGT 17450 0.0 20.019209 9 >>END_MODULE