FastQCFastQC Report
Thu 26 May 2016
SRR936716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461174
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99100.67822175866803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90840.6216918724258712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55870.3823637705023495No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28550.19539082956581488No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.16445679980618325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.031.2648331
GTATCAA134650.028.504371
AGGTAAG6000.024.5373542
GTACATG97650.024.4195651
TAAGGTG9400.024.2477365
GTAAGGT7150.023.9086064
TACATGG99800.023.4603062
TATCAAC166900.022.9917622
GAGTACT96850.022.80343412-13
ATCAACG168000.022.7831233
TCAACGC169350.022.5999574
AGTACTT98450.022.52932412-13
ACATGGG100800.022.3308543
CAACGCA171100.022.3133035
AACGCAG174150.022.0315826
GTACTTT101700.021.64368414-15
GTGTAAG6600.020.8718281
CATGGGA63200.020.4366464
TCGTAGC1404.8594814E-420.3528677
AAGGTGT11350.020.0818236
Produced by FastQC (version 0.11.5)