FastQCFastQC Report
Thu 26 May 2016
SRR936715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691679
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.5120356757990139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72240.42703136942646924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.24975187373018165No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.11544743417634196No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18850.11142775904885029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76150.032.132861
GTATCAA112450.030.6330761
GTACATG97150.027.4856221
TACATGG99850.026.3133582
ACATGGG98750.025.5945843
ATCAACG136450.025.1383723
TCAACGC137000.025.0381934
TATCAAC137900.024.9444242
CAACGCA138300.024.8028375
AACGCAG141800.024.4258986
TACCTGG13100.023.9371172
GTATCGA1000.001651343723.7564771
GAGTACT75750.022.86064112-13
GTACCTG15300.022.3590371
ACGCAGA157650.021.9393487
GTACCCG1302.9368812E-421.9290561
CATGGGG38600.021.785744
CGCAGAG158750.021.7580458
GTACTTT80450.021.37935314-15
CATGGGA55150.021.2783414
Produced by FastQC (version 0.11.5)