##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936715_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1691679 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394774067657043 31.0 31.0 34.0 30.0 34.0 2 31.461005900055508 31.0 31.0 34.0 28.0 34.0 3 31.454741118143573 31.0 31.0 34.0 28.0 34.0 4 35.116154424095825 37.0 35.0 37.0 32.0 37.0 5 35.13382858095419 37.0 35.0 37.0 32.0 37.0 6 35.14351126898188 37.0 35.0 37.0 32.0 37.0 7 35.06070300571208 37.0 35.0 37.0 32.0 37.0 8 35.014272211217374 37.0 35.0 37.0 32.0 37.0 9 36.5043214463264 39.0 37.0 39.0 32.0 39.0 10-11 36.367314957506714 39.0 36.0 39.0 32.0 39.0 12-13 36.25722817390297 39.0 35.0 39.0 32.0 39.0 14-15 37.311801175045616 39.5 37.0 41.0 32.0 41.0 16-17 37.19656979840738 39.0 36.0 41.0 31.0 41.0 18-19 37.18791656100241 39.0 36.0 41.0 31.0 41.0 20-21 37.07047909207361 39.0 36.0 41.0 31.0 41.0 22-23 37.1778954517967 39.0 36.0 41.0 31.0 41.0 24-25 37.211139347358454 39.0 36.0 41.0 31.0 41.0 26-27 37.11097672785439 39.0 36.0 41.0 31.0 41.0 28-29 36.958130058953266 39.0 36.0 41.0 30.5 41.0 30-31 36.71951800548449 39.0 36.0 41.0 30.0 41.0 32-33 36.49737863980105 39.0 35.0 40.5 30.0 41.0 34-35 36.2960685803867 39.0 35.0 40.0 30.0 41.0 36-37 36.072062430283765 38.0 35.0 40.0 29.5 41.0 38-39 35.850270057144414 38.0 35.0 40.0 29.0 41.0 40-41 35.619771245017525 38.0 35.0 40.0 27.5 41.0 42-43 35.350831629404865 38.0 34.5 40.0 27.0 41.0 44-45 35.03027879402653 38.0 34.0 40.0 25.5 41.0 46-47 34.74094701181489 38.0 34.0 40.0 24.5 41.0 48-49 34.52156407923725 38.0 34.0 40.0 24.0 41.0 50-51 33.53642948810028 37.5 32.0 39.5 22.5 40.5 52-53 33.62939304678961 37.5 33.0 39.5 23.0 40.5 54-55 34.057181060945965 38.0 33.0 40.0 23.0 41.0 56-57 33.95465717786885 38.0 33.0 40.0 22.0 41.0 58-59 33.720231497819626 37.5 33.0 40.0 21.5 41.0 60-61 33.42588251080731 37.0 33.0 40.0 20.0 41.0 62-63 33.05047647928478 36.5 32.0 40.0 18.5 41.0 64-65 32.68760060271482 36.0 32.0 39.0 18.0 41.0 66-67 32.2437338880485 35.5 31.0 39.0 13.5 41.0 68-69 31.856639468835397 35.0 31.0 39.0 12.5 40.0 70-71 31.463439872458075 35.0 31.0 38.0 9.0 40.0 72-73 31.067758422253867 35.0 30.5 37.0 7.5 39.5 74-75 30.634801874350867 35.0 30.0 37.0 6.5 39.0 76-77 30.17552000113497 34.5 29.5 36.0 2.0 39.0 78-79 29.684362695286755 34.0 29.0 36.0 2.0 38.5 80-81 29.230998020309997 34.0 29.0 35.0 2.0 37.0 82-83 28.809921090230475 34.0 29.0 35.0 2.0 37.0 84-85 28.427963874943178 34.0 28.0 35.0 2.0 36.0 86-87 28.043570027174187 34.0 27.0 35.0 2.0 36.0 88-89 27.621983544159384 33.5 26.0 35.0 2.0 36.0 90-91 27.196199456279828 33.0 26.0 35.0 2.0 35.0 92-93 26.728143459840787 33.0 25.0 35.0 2.0 35.0 94-95 26.20953325069354 33.0 24.5 35.0 2.0 35.0 96-97 25.578984547304778 32.5 21.5 35.0 2.0 35.0 98-99 24.777007044480662 32.0 10.0 35.0 2.0 35.0 100-101 21.816241142675413 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3766638688654709 1101 2 0.42403059267175536 1101 3 0.4388551213893095 1101 4 0.4387438084438955 1101 5 0.4465320023034707 1101 6 0.508546020249149 1101 7 0.4265926800241431 1101 8 0.5222158705358666 1101 9 0.5621564473353189 1101 10-11 0.7088990564306101 1101 12-13 0.7382452521753677 1101 14-15 0.8869849327477652 1101 16-17 0.8094510606988408 1101 18-19 0.769655854762398 1101 20-21 0.8154107267372837 1101 22-23 0.7346838988649864 1101 24-25 0.6439021500828233 1101 26-27 0.6586547718226043 1101 28-29 0.7958887357623681 1101 30-31 0.5491683948782295 1101 32-33 0.5088906559276296 1101 34-35 0.5421908576118142 1101 36-37 0.5438569016240251 1101 38-39 0.41821415096038805 1101 40-41 0.5333597592653803 1101 42-43 0.6637825010202647 1101 44-45 0.7997301054505712 1101 46-47 0.7254256624605233 1101 48-49 0.7925343361686004 1101 50-51 0.6320095824144616 1101 52-53 0.8329248940236127 1101 54-55 0.6847284656037544 1101 56-57 0.679219284725697 1101 58-59 0.7405192749525469 1101 60-61 0.8366166742794761 1101 62-63 0.8365910184446861 1101 64-65 0.7822124076969104 1101 66-67 0.8655758090484014 1101 68-69 0.7301025811083868 1101 70-71 0.5739330654792489 1101 72-73 0.49612240216836057 1101 74-75 0.4750535638404223 1101 76-77 0.4406687089986967 1101 78-79 0.35288146325133596 1101 80-81 0.3987107391338647 1101 82-83 0.39823985470375334 1101 84-85 0.3824364236543403 1101 86-87 0.2734086388986974 1101 88-89 0.3651078600844677 1101 90-91 0.47489989011210554 1101 92-93 0.5591071988160436 1101 94-95 0.5540084625170003 1101 96-97 0.45525135907579184 1101 98-99 0.26484179296633314 1101 100-101 0.7999959582848604 1102 1 0.4243087538495516 1102 2 0.43808682774197294 1102 3 0.42160922290518954 1102 4 0.3089150739957205 1102 5 0.42350887502779955 1102 6 0.5436595420035033 1102 7 0.44125797746120554 1102 8 0.45354408867390106 1102 9 0.43197094922423673 1102 10-11 0.5848138370826845 1102 12-13 0.5373928497662206 1102 14-15 0.6032077710670265 1102 16-17 0.5556243193154558 1102 18-19 0.5052377910615675 1102 20-21 0.5126518071708688 1102 22-23 0.42870951620540865 1102 24-25 0.3011306187468179 1102 26-27 0.22932009331826464 1102 28-29 0.07659874293563718 1102 30-31 0.5085935716930905 1102 32-33 0.3272558489630484 1102 34-35 0.3717146493479433 1102 36-37 0.05964092380352781 1102 38-39 0.13545457294816998 1102 40-41 0.16401265892773864 1102 42-43 0.09243087640021486 1102 44-45 0.21862636035013594 1102 46-47 0.13367899568411445 1102 48-49 0.4350886437020449 1102 50-51 0.2570576204675632 1102 52-53 0.256821854853051 1102 54-55 0.24328012542532207 1102 56-57 0.36094985123600765 1102 58-59 0.3744903615843853 1102 60-61 0.3536063170536323 1102 62-63 0.39489829357436435 1102 64-65 0.5582773646415191 1102 66-67 0.5055221330531126 1102 68-69 0.5835640433519025 1102 70-71 0.5772496696463243 1102 72-73 0.29375953142063693 1102 74-75 0.2425579339578725 1102 76-77 0.26103313975647424 1102 78-79 0.2186408253693024 1102 80-81 0.3549967439316539 1102 82-83 0.3582530426134234 1102 84-85 0.07137380609169242 1102 86-87 0.31540165304198453 1102 88-89 0.4105768665973848 1102 90-91 0.2683544639995361 1102 92-93 0.21333008398626063 1102 94-95 0.14574373311606692 1102 96-97 0.3255494071005529 1102 98-99 0.3343173218914153 1102 100-101 0.5191048785160497 1103 1 0.3749455961086241 1103 2 0.28216790919718093 1103 3 0.3438583273577791 1103 4 0.3900740484651237 1103 5 0.4700518082397309 1103 6 0.47357474762180374 1103 7 0.4890553691841788 1103 8 0.41112290376739224 1103 9 0.32203541018083115 1103 10-11 0.5701550064238177 1103 12-13 0.24410964760551934 1103 14-15 0.6218323886617512 1103 16-17 0.6648852562218508 1103 18-19 0.7138055150560803 1103 20-21 0.7237302938841665 1103 22-23 0.45741563668941154 1103 24-25 0.5662878644136597 1103 26-27 0.44167687879140516 1103 28-29 0.48464921043522935 1103 30-31 0.4176267188674032 1103 32-33 0.5543396020641964 1103 34-35 0.6894365674574559 1103 36-37 0.8150925734718371 1103 38-39 0.7108734307387792 1103 40-41 0.5473881613033029 1103 42-43 0.538041732788102 1103 44-45 0.780192649315488 1103 46-47 0.8724932278512227 1103 48-49 0.7418801922569429 1103 50-51 0.8586529176324191 1103 52-53 0.8726179422837035 1103 54-55 0.8515408485385194 1103 56-57 0.9178723673512437 1103 58-59 0.968737325028826 1103 60-61 0.9854410472204691 1103 62-63 1.0056024114774615 1103 64-65 0.9173493483501787 1103 66-67 1.1720850305508108 1103 68-69 0.951111466220997 1103 70-71 0.9271025522400187 1103 72-73 0.8819981369352767 1103 74-75 1.0068864618148154 1103 76-77 0.8654202069598291 1103 78-79 0.8924036318126305 1103 80-81 1.121977384111549 1103 82-83 0.875438785837023 1103 84-85 0.8880601345549515 1103 86-87 0.9504829636711989 1103 88-89 0.7851511643482496 1103 90-91 0.8871601136770764 1103 92-93 0.7624139262960874 1103 94-95 0.9334683121049636 1103 96-97 0.9671774038582797 1103 98-99 0.7954352603712671 1103 100-101 0.7018863741763575 1104 1 0.32793176394736534 1104 2 0.3413442468157015 1104 3 0.21748960054638644 1104 4 0.345411364268287 1104 5 0.43122440069372203 1104 6 0.5021904210288568 1104 7 0.5743559787241566 1104 8 0.4151853880801397 1104 9 0.4203464352941211 1104 10-11 0.5871224879908254 1104 12-13 0.5253769796397592 1104 14-15 0.41709419269928816 1104 16-17 0.30717838608109815 1104 18-19 0.3365522935604872 1104 20-21 0.22241750875651434 1104 22-23 0.40101744446906906 1104 24-25 0.3788594172878703 1104 26-27 0.1980295874945739 1104 28-29 0.16784282266313966 1104 30-31 0.15632786782060037 1104 32-33 0.2736758773748633 1104 34-35 0.1624561710054735 1104 36-37 0.12166790801907723 1104 38-39 -0.22470182927025206 1104 40-41 -0.39531523292318127 1104 42-43 -0.10216660910191422 1104 44-45 -0.08964615040644475 1104 46-47 -0.40185178668372146 1104 48-49 -0.08067537293280225 1104 50-51 -0.18423971236449432 1104 52-53 -0.03474339003347637 1104 54-55 -0.2624568179592899 1104 56-57 -0.18670214879691827 1104 58-59 -0.259184078236558 1104 60-61 -0.14485143157036617 1104 62-63 -0.11108486728477374 1104 64-65 -0.15746966832356435 1104 66-67 -0.03361562097395421 1104 68-69 -0.1610183336971609 1104 70-71 -0.2868786854288494 1104 72-73 -0.3169744527805811 1104 74-75 -0.20770842303886639 1104 76-77 -0.24303425095726894 1104 78-79 -0.08712011469053849 1104 80-81 -0.05109865550626935 1104 82-83 -0.1540471591476944 1104 84-85 -0.31517527991550054 1104 86-87 -0.4426385290400283 1104 88-89 -0.5133478795851758 1104 90-91 -0.5302611382301095 1104 92-93 -0.7699309391624531 1104 94-95 -0.7042209426803119 1104 96-97 -0.49751886816661894 1104 98-99 -0.43581480618070145 1104 100-101 -0.5563465227281768 1105 1 0.23947296673607 1105 2 0.19233168110433851 1105 3 0.23299878553224573 1105 4 0.3617986481027273 1105 5 0.31553285515885676 1105 6 0.2828715273435307 1105 7 0.37098756637540475 1105 8 0.4085021301087224 1105 9 0.2953265499952238 1105 10-11 0.4030497810466471 1105 12-13 0.3063456925373842 1105 14-15 0.36967775364626476 1105 16-17 0.32852553531565576 1105 18-19 0.32259618412258817 1105 20-21 0.14329738717082563 1105 22-23 0.0361925384580033 1105 24-25 0.1567610756094524 1105 26-27 0.08880289868219648 1105 28-29 0.08409942525644709 1105 30-31 -0.1371027484394176 1105 32-33 -0.15250989310968777 1105 34-35 -0.0822381616169352 1105 36-37 0.1295928175353538 1105 38-39 -0.07783058444605473 1105 40-41 -0.02750820615509042 1105 42-43 -0.08917263356105565 1105 44-45 0.2257279493624864 1105 46-47 0.24577802050302466 1105 48-49 0.03932757503466888 1105 50-51 0.12668996692720924 1105 52-53 0.2467812124627713 1105 54-55 0.16105690761548885 1105 56-57 0.052963562476627146 1105 58-59 0.32153136111395497 1105 60-61 0.2964287412811828 1105 62-63 0.255546118190189 1105 64-65 0.34551110427759113 1105 66-67 0.21783432065090125 1105 68-69 0.32218094800539987 1105 70-71 0.07854570055356547 1105 72-73 -0.11967993195561988 1105 74-75 0.08020329252541458 1105 76-77 -0.08465739465900057 1105 78-79 -0.018328664515234294 1105 80-81 0.11315301463414684 1105 82-83 0.08747406397120372 1105 84-85 -0.0863181835376352 1105 86-87 0.042392447087724605 1105 88-89 -0.089776183308512 1105 90-91 -0.07313294388715263 1105 92-93 -0.28815890512780484 1105 94-95 -0.19836814871717579 1105 96-97 -0.18557537567000892 1105 98-99 -0.1312893165979041 1105 100-101 -0.040979792877937626 1106 1 0.3821199012619374 1106 2 0.18747548445910667 1106 3 0.2934515182954378 1106 4 0.36689688619467375 1106 5 0.25049286069123866 1106 6 0.38268480965606244 1106 7 0.520123516829905 1106 8 0.544733045332741 1106 9 0.5762675149337895 1106 10-11 0.7018691566565565 1106 12-13 0.7167769105053168 1106 14-15 0.5286932212472877 1106 16-17 0.4254400831591809 1106 18-19 0.31941890989347144 1106 20-21 0.12422413697681378 1106 22-23 0.3934514767028574 1106 24-25 0.20943198376433259 1106 26-27 0.18492408845038 1106 28-29 0.19081857265585 1106 30-31 0.3605325450454373 1106 32-33 0.3326564239415575 1106 34-35 0.27271177806480296 1106 36-37 0.13975471636970838 1106 38-39 0.1514210848715507 1106 40-41 0.06497083612916299 1106 42-43 0.06382061233000513 1106 44-45 0.06352368174916734 1106 46-47 0.28524806499254396 1106 48-49 0.374097789337398 1106 50-51 0.05494196665058837 1106 52-53 0.029443361022437387 1106 54-55 0.30462590479551466 1106 56-57 0.46489505322141866 1106 58-59 0.34777172748018614 1106 60-61 0.4629340469833778 1106 62-63 0.5257815515212272 1106 64-65 0.49389787882559233 1106 66-67 0.5081726408064213 1106 68-69 0.5273667820494872 1106 70-71 0.41030768544710483 1106 72-73 0.21801206838246756 1106 74-75 0.30181913048826914 1106 76-77 0.31992256738275415 1106 78-79 0.2317616207863189 1106 80-81 0.5125675048487572 1106 82-83 0.46935975893750737 1106 84-85 0.5772442055267035 1106 86-87 0.5246350571397471 1106 88-89 0.46810629980621243 1106 90-91 0.4760994389532627 1106 92-93 0.39464577895047626 1106 94-95 0.42417397802033463 1106 96-97 0.25874740772979266 1106 98-99 0.33239486157533804 1106 100-101 0.31344764463310426 1107 1 0.27241943671906554 1107 2 0.2517703827219826 1107 3 0.3096446429667914 1107 4 0.37761348680896845 1107 5 0.2186242078655738 1107 6 0.4054096494658239 1107 7 0.3800807210087953 1107 8 0.41711903587719235 1107 9 0.3830052099588599 1107 10-11 0.4920034729527316 1107 12-13 0.39815243501291775 1107 14-15 0.24046548348521668 1107 16-17 0.3349206868178882 1107 18-19 0.3298922989625339 1107 20-21 0.42414249788980385 1107 22-23 0.38261782869436445 1107 24-25 0.2561335999594547 1107 26-27 0.39476036326119157 1107 28-29 0.4801376744376782 1107 30-31 0.5090683350800589 1107 32-33 0.2637831492142624 1107 34-35 0.4682144525095353 1107 36-37 0.44226841209118817 1107 38-39 0.44454422696473017 1107 40-41 0.5346293871177039 1107 42-43 0.4721039799540989 1107 44-45 0.3451482407628177 1107 46-47 0.3648064728186142 1107 48-49 0.5527065251902528 1107 50-51 0.6075000698005155 1107 52-53 0.6475641798130667 1107 54-55 0.7497045030029881 1107 56-57 0.40852732207046927 1107 58-59 0.4472708352143684 1107 60-61 0.5709555578240852 1107 62-63 0.6181825288375578 1107 64-65 0.5490648448747848 1107 66-67 0.7320404866520605 1107 68-69 0.8522050625468047 1107 70-71 0.6480100627443797 1107 72-73 0.5124889018210865 1107 74-75 0.5377713845622836 1107 76-77 0.5586939579410846 1107 78-79 0.4038086444298905 1107 80-81 0.6188271289090004 1107 82-83 0.6036834073341915 1107 84-85 0.29570493230279027 1107 86-87 0.47578335677637185 1107 88-89 0.5466206919302756 1107 90-91 0.33135827838423637 1107 92-93 0.4750632205414753 1107 94-95 0.6273611962096197 1107 96-97 0.5755738235033121 1107 98-99 0.5244005245517762 1107 100-101 0.2324252621128089 1108 1 0.2127316869655509 1108 2 0.2162351372713509 1108 3 0.12843215554071463 1108 4 0.3953960837346173 1108 5 0.1742497800415066 1108 6 0.18398585552021274 1108 7 0.1764075203057729 1108 8 0.1610384829005298 1108 9 0.14954597589801466 1108 10-11 0.321152251322232 1108 12-13 0.2099108817831592 1108 14-15 0.37276386468607114 1108 16-17 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2316 62-63 -1.594395129868893 2316 64-65 -1.5070136554689348 2316 66-67 -1.4986508444840183 2316 68-69 -1.7533389017574912 2316 70-71 -1.5076281842086985 2316 72-73 -1.6267354105741099 2316 74-75 -1.3811909120152706 2316 76-77 -1.5126311870967832 2316 78-79 -2.1608335954817015 2316 80-81 -1.8711728068438838 2316 82-83 -1.7065767774405245 2316 84-85 -1.7961098239757582 2316 86-87 -1.6714646178331698 2316 88-89 -2.426306776283461 2316 90-91 -2.5192647580631586 2316 92-93 -3.141284298555984 2316 94-95 -2.9312618916354687 2316 96-97 -3.087672085561316 2316 98-99 -4.569570927240395 2316 100-101 -5.4829937254810694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3643.0 3 1488.0 4 1500.0 5 1662.0 6 1987.0 7 2381.0 8 2990.0 9 3662.0 10 4758.0 11 5937.0 12 7948.0 13 9408.0 14 10863.0 15 13761.0 16 16544.0 17 21284.0 18 16705.0 19 12008.0 20 12008.0 21 12786.0 22 14038.0 23 16004.0 24 18147.0 25 20587.0 26 24256.0 27 28315.0 28 33919.0 29 41126.0 30 49876.0 31 61170.0 32 76015.0 33 97097.0 34 126588.0 35 169426.0 36 228094.0 37 284746.0 38 209799.0 39 29136.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.48603180871731 18.83184371611707 14.231332686170623 22.450791788995 2 15.772895280120888 20.25339879064774 39.916014576549344 24.057691352682024 3 19.34729013071934 25.112716609219525 29.811872934415668 25.728120325645463 4 12.601410674060979 16.602025552947573 37.27230266230733 33.52426111068411 5 13.454480303269515 37.86144377055161 33.70845846866871 14.97561745751016 6 31.326327966152817 36.16962387943892 17.499086282167895 15.004961872240367 7 28.528586047221026 33.44967447535643 20.36354242224859 17.65819705517396 8 26.97559461337196 33.85102514449586 19.74728377359606 19.42609646853612 9 27.259491941769276 16.427626124586503 20.460466948067076 35.85241498557715 10-11 25.27479870763665 26.709414282170336 26.64604980276586 21.369737207427157 12-13 26.369997676191133 24.3653429628504 27.379612804955567 21.88504655600291 14-15 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29.917513853109163 21.776425537247633 40-41 21.894529633614322 26.460931865741344 30.02184245061064 21.622696050033692 42-43 21.82637574803131 26.57638564985839 29.539987715822885 22.05725088628742 44-45 21.896830527055332 26.804307146824097 29.34475056505366 21.95411176106691 46-47 22.234957900831755 27.094502709024653 28.740459447980253 21.930079942163335 48-49 22.288028487893 27.269596953535746 28.344631859953438 22.09774269861781 50-51 22.182582165757967 27.414310973385724 28.300950986958583 22.102155873897722 52-53 22.511803794830627 27.40020347395421 28.127318417821527 21.960674313393636 54-55 22.070613216930408 27.34604697163122 28.28920980222518 22.294130009213195 56-57 22.150232152457765 27.750058595995608 28.018787368801867 22.080921882744757 58-59 22.37554338878335 27.22245162594693 28.08755819621083 22.314446789058888 60-61 22.13344864604122 27.305052162645303 28.607758661458284 21.953740529855192 62-63 22.113887172138934 27.389132189549294 28.53191130279597 21.965069335515796 64-65 21.88754307873285 27.41300017142823 28.383431756784717 22.3160249930542 66-67 21.81989207737746 27.71478251409929 28.001172994867556 22.464152413655693 68-69 21.92234396275538 27.469562813334854 28.381281296524357 22.22681192738541 70-71 21.951725832679557 27.513815030149548 28.198334359954615 22.33612477721628 72-73 22.07803072382523 27.48826653998836 28.137187404573723 22.296515331612692 74-75 21.88526196874286 27.98759198907499 27.86938948317004 22.257756559012112 76-77 22.0746143339463 27.693483441045867 27.956901190257977 22.275001034749856 78-79 22.156676380252893 27.647612699385306 27.84746377218752 22.348247148174284 80-81 22.030296101486705 27.995482591026295 27.737459028443528 22.236762279043475 82-83 22.06207150450433 27.998178455205817 27.669975116397005 22.269774923892843 84-85 21.964132891288635 27.932652726393943 27.58474206750986 22.51847231480756 86-87 22.15302304365165 27.892074508073065 27.671361088750384 22.283541359524893 88-89 22.12092365999237 27.90438209322332 27.440748766492344 22.533945480291962 90-91 22.039442463257195 27.89676563995469 27.717290871239125 22.34650102554899 92-93 22.201800253834193 27.776869514264945 27.676855412365263 22.344474819535595 94-95 22.02757245025188 27.50103312442247 27.772956202027704 22.698438223297945 96-97 22.188374011787452 27.36791881395871 27.73522363024865 22.708483544005187 98-99 21.90697268677394 27.568068675619774 27.52179010821496 23.003168529391328 100-101 22.09640558976029 27.44095062244333 27.554049541703897 22.90859424609248 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 562.0 1 404.5 2 367.0 3 515.5 4 485.5 5 463.0 6 778.0 7 1506.0 8 2442.5 9 2918.5 10 2556.0 11 2137.5 12 2210.5 13 2397.0 14 2539.5 15 2660.0 16 2770.5 17 2964.5 18 3122.0 19 3347.5 20 3704.0 21 4266.0 22 4968.5 23 5893.5 24 7039.0 25 8490.0 26 10711.0 27 13197.5 28 15137.0 29 17578.5 30 21072.0 31 24781.0 32 29530.0 33 33368.5 34 36714.5 35 39381.5 36 42707.0 37 46877.5 38 48671.0 39 49545.5 40 49839.5 41 51297.5 42 52829.0 43 54353.0 44 56525.5 45 58947.5 46 59136.0 47 59253.5 48 64349.5 49 64227.0 50 58659.5 51 55247.5 52 52822.0 53 52605.5 54 52126.0 55 48750.5 56 44919.5 57 40761.0 58 37335.0 59 33517.5 60 28400.5 61 23981.0 62 20315.5 63 16152.0 64 12326.5 65 9998.0 66 7791.0 67 6224.5 68 4665.5 69 3153.0 70 2335.0 71 1817.0 72 1519.0 73 1323.0 74 1091.5 75 895.0 76 639.5 77 374.0 78 230.5 79 151.5 80 89.5 81 59.5 82 44.0 83 22.0 84 16.5 85 16.5 86 13.0 87 9.0 88 5.5 89 3.5 90 3.5 91 1.5 92 1.5 93 2.5 94 2.0 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011408783817733742 2 0.011527009556777617 3 0.015901361901400915 4 0.017083619291839644 5 0.030147563456187607 6 0.04640360257472014 7 0.006029512691237522 8 0.02683724276295917 9 0.03623618901694707 10-11 0.039516953275414546 12-13 0.02896530606574888 14-15 0.02719191998009078 16-17 0.028699298152900166 18-19 0.023999825025906214 20-21 0.05447250926446448 22-23 0.013241282772913775 24-25 0.003960562257969745 26-27 0.0015960474770922851 28-29 5.911286952193649E-5 30-31 0.002305401911355523 32-33 0.002128063302789714 34-35 0.0 36-37 4.1379008665355546E-4 38-39 5.911286952193649E-5 40-41 0.0011231445209167932 42-43 0.004256126605579428 44-45 0.0015073781728093805 46-47 0.001093588086155825 48-49 7.684673037851743E-4 50-51 0.004019675127491682 52-53 0.0036649979103600626 54-55 0.004935924605081697 56-57 6.206851299803332E-4 58-59 0.005645279039344935 60-61 0.002778304867531015 62-63 7.980237385461426E-4 64-65 5.320158256974284E-4 66-67 0.01666982920518609 68-69 0.041172113622028765 70-71 0.05068928561506054 72-73 0.015103338162854774 74-75 0.03035445849951439 76-77 0.026541678415349483 78-79 0.05438383996018156 80-81 0.07859056002941456 82-83 0.04767452926944178 84-85 0.017526965813254167 86-87 0.020423496419829056 88-89 0.02151708450598488 90-91 0.03744800284214676 92-93 0.024679623025408486 94-95 0.012295476860562789 96-97 0.01799986876942966 98-99 0.04915235100749019 100-101 0.06641330890789565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1691679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.08034037761449 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18903070287332 42.2848939420714 2 12.50163607453445 13.521854683757747 3 3.947520699970742 6.404497893062902 4 1.7225177634933986 3.72617387824841 5 0.9026861328483894 2.4408786659296715 6 0.5575631130950599 1.8091921762909924 7 0.35550584031227905 1.3458113795222542 8 0.2533677097057797 1.0961769585268148 9 0.19783760470230655 0.9629212501613418 >10 1.1737223058080157 12.145589956213056 >50 0.12556325008930405 4.640273000570203 >100 0.06876500145710548 6.6963454382707415 >500 0.00318539056888796 1.1664852416451994 >1k 8.787284327966785E-4 0.8152387562427416 >5k 2.1968210819916964E-4 0.943666779486461 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8662 0.5120356757990139 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7224 0.42703136942646924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.24975187373018165 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1953 0.11544743417634196 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1885 0.11142775904885029 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 5.911286952193649E-5 0.0 0.0 0.0 0.0 14-15 1.1822573904387297E-4 0.0 0.0 0.0 0.0 16-17 1.1822573904387297E-4 0.0 0.0 0.0 0.0 18-19 1.1822573904387297E-4 0.0 0.0 0.0 0.0 20-21 1.1822573904387297E-4 2.9556434760968244E-5 0.0 0.0 0.0 22-23 1.1822573904387297E-4 5.911286952193649E-5 0.0 1.1822573904387299E-4 0.0 24-25 1.7733860856580948E-4 5.911286952193649E-5 0.0 2.660079128487142E-4 0.0 26-27 1.7733860856580948E-4 1.1822573904387297E-4 0.0 4.433465214145237E-4 0.0 28-29 1.7733860856580948E-4 1.1822573904387297E-4 0.0 0.0011527009556777615 0.0 30-31 2.3645147808774595E-4 1.1822573904387297E-4 0.0 0.002778304867531015 0.0 32-33 2.3645147808774595E-4 1.1822573904387297E-4 0.0 0.006354633473608173 0.0 34-35 2.3645147808774595E-4 1.1822573904387297E-4 0.0 0.01043342147062179 0.0 36-37 2.3645147808774595E-4 1.1822573904387297E-4 0.0 0.014571322337157345 0.0 38-39 4.137900866535554E-4 1.1822573904387297E-4 0.0 0.02142841520170198 0.0 40-41 4.137900866535554E-4 1.1822573904387297E-4 0.0 0.032452965367543135 0.0 42-43 4.4334652141452365E-4 1.4778217380484124E-4 0.0 0.045310014488564324 0.0 44-45 4.729029561754919E-4 1.7733860856580948E-4 0.0 0.05934932100002424 0.0 46-47 5.911286952193649E-4 1.7733860856580948E-4 0.0 0.07652160959614679 0.0 48-49 5.911286952193649E-4 2.9556434760968247E-4 0.0 0.09395990610511805 0.0 50-51 5.911286952193649E-4 2.9556434760968247E-4 0.0 0.11621590148012714 0.0 52-53 5.911286952193649E-4 2.9556434760968247E-4 0.0 0.15419592014797134 0.0 54-55 5.911286952193649E-4 2.9556434760968247E-4 0.0 0.20039262767936472 0.0 56-57 5.911286952193649E-4 2.9556434760968247E-4 0.0 0.24508195703794872 0.0 58-59 5.911286952193649E-4 3.5467721713161897E-4 0.0 0.30582043047173846 0.0 60-61 5.911286952193649E-4 3.5467721713161897E-4 0.0 0.37977063024368096 0.0 62-63 6.502415647413014E-4 3.5467721713161897E-4 0.0 0.4409524501988853 0.0 64-65 6.502415647413014E-4 4.137900866535554E-4 0.0 0.4977599178094662 0.0 66-67 6.502415647413014E-4 4.137900866535554E-4 0.0 0.5887050675689656 0.0 68-69 6.502415647413014E-4 4.137900866535554E-4 0.0 0.6956698049689096 0.0 70-71 7.093544342632379E-4 4.4334652141452365E-4 0.0 0.782802174644244 0.0 72-73 7.093544342632379E-4 4.729029561754919E-4 0.0 0.8931067891721776 0.0 74-75 7.093544342632379E-4 4.729029561754919E-4 0.0 1.015411316213064 0.0 76-77 7.093544342632379E-4 4.729029561754919E-4 0.0 1.1489768448978797 0.0 78-79 7.093544342632379E-4 4.729029561754919E-4 0.0 1.297379703832701 0.0 80-81 7.093544342632379E-4 4.729029561754919E-4 0.0 1.4726493619652428 0.0 82-83 7.093544342632379E-4 5.615722604583967E-4 0.0 1.6655937680848436 0.0 84-85 7.389108690242062E-4 6.502415647413014E-4 0.0 1.8576514811616152 0.0 86-87 7.684673037851744E-4 6.502415647413014E-4 0.0 2.0782902666522434 0.0 88-89 7.684673037851744E-4 6.502415647413014E-4 0.0 2.328485486903839 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7615 0.0 32.13286 1 GTATCAA 11245 0.0 30.633076 1 GTACATG 9715 0.0 27.485622 1 TACATGG 9985 0.0 26.313358 2 ACATGGG 9875 0.0 25.594584 3 ATCAACG 13645 0.0 25.138372 3 TCAACGC 13700 0.0 25.038193 4 TATCAAC 13790 0.0 24.944424 2 CAACGCA 13830 0.0 24.802837 5 AACGCAG 14180 0.0 24.425898 6 TACCTGG 1310 0.0 23.937117 2 GTATCGA 100 0.0016513437 23.756477 1 GAGTACT 7575 0.0 22.860641 12-13 GTACCTG 1530 0.0 22.359037 1 ACGCAGA 15765 0.0 21.939348 7 GTACCCG 130 2.9368812E-4 21.929056 1 CATGGGG 3860 0.0 21.78574 4 CGCAGAG 15875 0.0 21.758045 8 GTACTTT 8045 0.0 21.379353 14-15 CATGGGA 5515 0.0 21.278341 4 >>END_MODULE