FastQCFastQC Report
Thu 26 May 2016
SRR936715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691679
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87510.5172967211864663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83110.4912870585968142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.28604717561665066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.1420482254612134No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20950.12384146164845695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85550.035.2064441
TCGCATA650.005848540529.2269869
GTATCAA122050.028.219751
GTACATG91250.023.8443551
ATCAACG150300.022.9424973
TCAACGC151600.022.777094
TATCAAC151350.022.7532962
GTACCTG14300.022.5904981
GAGTACT92300.022.43745412-13
CAACGCA154750.022.3134565
TACATGG95600.022.3088512
ACATGGG94950.021.8599663
AACGCAG158700.021.8166
GTACTTT95600.021.68842114-15
TACCTGG14250.021.3330942
GGACCGT1656.3327156E-520.148316
AGTACTT93650.019.85695812-13
GTATAGG6500.019.7334651
TGGACCG1707.960983E-519.5574475
GTAAGGT8050.019.470684
Produced by FastQC (version 0.11.5)