##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936715_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1691679 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53077800220964 31.0 31.0 34.0 30.0 34.0 2 31.65059033067148 31.0 31.0 34.0 30.0 34.0 3 31.746240273716232 31.0 31.0 34.0 30.0 34.0 4 35.56720039676558 37.0 35.0 37.0 33.0 37.0 5 35.3944087501234 37.0 35.0 37.0 33.0 37.0 6 35.45097444609763 37.0 35.0 37.0 33.0 37.0 7 35.3598850609365 37.0 35.0 37.0 33.0 37.0 8 35.3237292654221 37.0 35.0 37.0 32.0 37.0 9 36.84653235040454 39.0 37.0 39.0 33.0 39.0 10-11 36.723740142190096 39.0 35.0 39.0 32.0 39.0 12-13 36.566746114363305 38.0 35.0 39.0 32.0 39.0 14-15 37.528097233576815 39.0 36.0 41.0 32.0 41.0 16-17 37.491679272486095 39.0 36.0 40.5 32.0 41.0 18-19 37.67463774155735 39.0 36.5 41.0 32.0 41.0 20-21 37.694923504991195 39.0 37.0 41.0 32.0 41.0 22-23 37.58330244685901 39.0 36.0 41.0 32.0 41.0 24-25 37.44981317377588 39.0 36.0 41.0 32.0 41.0 26-27 37.19758594863446 39.0 36.0 41.0 31.0 41.0 28-29 37.1647522963872 39.0 36.0 41.0 31.0 41.0 30-31 36.992733846078366 39.0 36.0 40.0 31.0 41.0 32-33 36.74174799119692 39.0 35.0 40.0 30.0 41.0 34-35 36.52750817383203 38.5 35.0 40.0 30.0 41.0 36-37 36.29849013908667 38.0 35.0 40.0 30.0 41.0 38-39 36.05924144001315 38.0 35.0 40.0 30.0 41.0 40-41 35.791280142391074 38.0 35.0 40.0 29.0 41.0 42-43 35.465769806210275 38.0 34.0 40.0 27.5 41.0 44-45 35.11568802355529 38.0 34.0 40.0 26.5 41.0 46-47 34.65831756497539 38.0 33.5 40.0 24.5 41.0 48-49 34.183793142788915 38.0 33.0 40.0 23.5 41.0 50-51 33.828628540048086 38.0 33.0 40.0 23.0 41.0 52-53 33.51465023801797 37.0 32.5 40.0 22.0 41.0 54-55 33.13222928226927 37.0 32.0 40.0 20.0 41.0 56-57 32.75569301268148 36.5 31.0 39.0 18.5 40.0 58-59 32.35490893958014 36.0 31.0 39.0 18.0 40.0 60-61 31.941077178353574 35.5 30.5 39.0 15.0 40.0 62-63 31.56533568129651 35.0 30.0 38.5 13.0 40.0 64-65 31.197744962253477 35.0 29.5 38.0 9.5 40.0 66-67 30.691629144772737 34.5 29.0 38.0 8.0 40.0 68-69 30.215592024255194 34.0 28.5 37.0 7.0 39.5 70-71 29.672848986125615 34.0 28.0 36.5 4.0 39.0 72-73 29.053180301936713 33.5 26.5 36.0 2.0 39.0 74-75 28.46581916545633 33.0 26.0 35.5 2.0 38.0 76-77 27.200525336071443 31.5 25.5 34.0 2.0 36.0 78-79 27.957398832757278 32.5 26.0 35.0 2.0 37.0 80-81 27.771637822541983 33.0 26.0 35.0 2.0 36.5 82-83 27.306486336946904 32.5 26.0 35.0 2.0 36.0 84-85 26.78770529160674 32.0 25.0 35.0 2.0 36.0 86-87 26.27093497052337 32.0 24.5 34.0 2.0 35.0 88-89 25.65255731140482 31.5 22.5 34.0 2.0 35.0 90-91 24.956839033882908 31.0 19.5 34.0 2.0 35.0 92-93 24.22986778224474 31.0 17.0 34.0 2.0 35.0 94-95 23.320797562658164 30.0 4.5 34.0 2.0 35.0 96-97 22.31455731258708 30.0 2.0 34.0 2.0 35.0 98-99 21.00092866318019 29.0 2.0 34.0 2.0 35.0 100-101 17.305561220538884 22.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30533511378530775 1101 2 0.4700190603085126 1101 3 0.2768882752859554 1101 4 0.2191950558980622 1101 5 0.3150109957620302 1101 6 0.33734248246279464 1101 7 0.3535022477541503 1101 8 0.24300251472767087 1101 9 0.4273798358944987 1101 10-11 0.41296297059682985 1101 12-13 0.45899804665252475 1101 14-15 0.5005348954914624 1101 16-17 0.42062397873094426 1101 18-19 0.4973863134597565 1101 20-21 0.4239213797948693 1101 22-23 0.5958775631392967 1101 24-25 0.4166888101455939 1101 26-27 0.33252015972931304 1101 28-29 0.4054782139945772 1101 30-31 0.6332981078605968 1101 32-33 0.5128178566643129 1101 34-35 0.6744549042440156 1101 36-37 0.7586961158002765 1101 38-39 0.7657341973839351 1101 40-41 0.6444721171463073 1101 42-43 0.737732958125207 1101 44-45 0.6933636233387617 1101 46-47 0.7100546483348893 1101 48-49 0.7869310998949715 1101 50-51 0.832767851542485 1101 52-53 0.6685821896869228 1101 54-55 0.8966756579407118 1101 56-57 0.8524612058196084 1101 58-59 0.8322129891872265 1101 60-61 0.6534893251760927 1101 62-63 0.622110308558657 1101 64-65 0.6990617352994057 1101 66-67 0.7538653563905449 1101 68-69 0.6894119606539988 1101 70-71 0.6463435030392688 1101 72-73 0.821963617067226 1101 74-75 0.8084867096689479 1101 76-77 0.7638267264261991 1101 78-79 0.9153323752507028 1101 80-81 1.076804782672447 1101 82-83 0.8766317580839278 1101 84-85 0.8407907721518519 1101 86-87 0.9080774335687352 1101 88-89 0.9531135375328788 1101 90-91 1.0443759159886135 1101 92-93 1.057833683875824 1101 94-95 0.9846680888165693 1101 96-97 1.1662446412872107 1101 98-99 0.5253153052465827 1101 100-101 0.606114648899851 1102 1 0.34947409143423513 1102 2 0.4546446673923761 1102 3 0.43525746964586176 1102 4 0.27219361174914525 1102 5 0.3266642202154415 1102 6 0.2955872052412829 1102 7 0.3523996675962877 1102 8 0.3034098520931394 1102 9 0.30684405094795864 1102 10-11 0.5682252821088056 1102 12-13 0.5124431110630852 1102 14-15 0.5997482743504108 1102 16-17 0.6109038941713081 1102 18-19 0.5541142683460833 1102 20-21 0.4187646095475017 1102 22-23 0.42978271022279557 1102 24-25 0.4820607655460165 1102 26-27 0.5321213568344234 1102 28-29 0.7349375856726681 1102 30-31 0.8136855082469339 1102 32-33 0.6597603255954851 1102 34-35 0.7181393010411838 1102 36-37 0.8852376862061746 1102 38-39 0.8572033988981005 1102 40-41 0.9133739435103507 1102 42-43 0.9450542739784922 1102 44-45 0.9055473081914087 1102 46-47 0.8860996303131046 1102 48-49 1.06023739052155 1102 50-51 1.1918579174313848 1102 52-53 1.2064269952776385 1102 54-55 1.2203231374544927 1102 56-57 1.3113178400092806 1102 58-59 1.1949541053921493 1102 60-61 1.080198767605463 1102 62-63 1.0118454867220628 1102 64-65 1.1214802003989313 1102 66-67 1.1344661818536572 1102 68-69 1.1935829615612619 1102 70-71 0.9983875657258388 1102 72-73 0.8820437891748298 1102 74-75 1.0949896153776066 1102 76-77 1.0894875306037868 1102 78-79 1.1520680162255346 1102 80-81 1.3231013415967006 1102 82-83 1.3844834077122528 1102 84-85 1.2161124767414115 1102 86-87 1.2525528516976472 1102 88-89 1.1255968975311568 1102 90-91 1.193473212118917 1102 92-93 1.3414467089467834 1102 94-95 1.376199589433547 1102 96-97 1.3932553859830783 1102 98-99 1.2780253795306677 1102 100-101 0.9627687818818416 1103 1 0.3769236000944005 1103 2 0.43118893873439745 1103 3 0.3351468816530385 1103 4 0.2304636402497806 1103 5 0.2502895991600411 1103 6 0.34826646468625455 1103 7 0.2771626109287908 1103 8 0.28043369695618026 1103 9 0.1780095007365503 1103 10-11 0.33992706059136424 1103 12-13 0.3760485872451369 1103 14-15 0.4823861132856848 1103 16-17 0.5932991778010575 1103 18-19 0.5046359083706307 1103 20-21 0.3579090676125034 1103 22-23 0.41233918469604447 1103 24-25 0.4897362490031938 1103 26-27 0.5360631497134207 1103 28-29 0.4817518362967448 1103 30-31 0.747693853872093 1103 32-33 0.6068688110321645 1103 34-35 0.44731345944195056 1103 36-37 0.5655148790860878 1103 38-39 0.443909922359218 1103 40-41 0.5066563766307652 1103 42-43 0.6672510836917809 1103 44-45 0.6649058363098277 1103 46-47 0.6634218628062811 1103 48-49 0.6686716091227822 1103 50-51 0.6376276575419126 1103 52-53 0.8409707467484253 1103 54-55 0.8967032955999414 1103 56-57 0.8802615324177765 1103 58-59 0.8090932186271758 1103 60-61 0.5304055625377906 1103 62-63 0.7794164422238445 1103 64-65 0.9065534361051597 1103 66-67 0.7984467958861821 1103 68-69 0.7263614579644582 1103 70-71 0.544989099807399 1103 72-73 0.8995191529693365 1103 74-75 0.8055171616315349 1103 76-77 0.4693111435242763 1103 78-79 0.6520083386995061 1103 80-81 0.9067344724673632 1103 82-83 0.656789498049875 1103 84-85 0.960036058746482 1103 86-87 0.8430089079667873 1103 88-89 0.7531520723263228 1103 90-91 1.0534905001444983 1103 92-93 1.074225974390263 1103 94-95 1.1458051179103386 1103 96-97 1.1897510232175499 1103 98-99 1.342456497361482 1103 100-101 0.796237107419671 1104 1 0.38392319393723895 1104 2 0.433948373521833 1104 3 0.36524206237776013 1104 4 0.26746379693212674 1104 5 0.11014529927915362 1104 6 0.18928864208413643 1104 7 0.16952518301408048 1104 8 0.19338250442095273 1104 9 0.3251132351006447 1104 10-11 0.28684612274862786 1104 12-13 0.265073216797866 1104 14-15 0.4261109851636391 1104 16-17 0.39707491820121277 1104 18-19 0.4173530389235367 1104 20-21 0.43072437563022703 1104 22-23 0.2694708937049768 1104 24-25 0.26392306000015964 1104 26-27 0.26033370210237194 1104 28-29 0.4488302885185149 1104 30-31 0.42100309158963967 1104 32-33 0.2817559769222626 1104 34-35 0.31577619262103696 1104 36-37 0.4418009293370133 1104 38-39 0.551891138731456 1104 40-41 0.5008939648786779 1104 42-43 0.5292531798886557 1104 44-45 0.56876022212262 1104 46-47 0.4269195909122061 1104 48-49 0.33158215871274876 1104 50-51 0.4953021263811408 1104 52-53 0.4221092341704846 1104 54-55 0.34625994539663196 1104 56-57 0.566791109634039 1104 58-59 0.7274118192560408 1104 60-61 0.4902022405677364 1104 62-63 0.4433819355983175 1104 64-65 0.4758382192065902 1104 66-67 0.4589719707847557 1104 68-69 0.4007910505447114 1104 70-71 0.1689415718137397 1104 72-73 0.3470164186341478 1104 74-75 0.5002675423087979 1104 76-77 0.26461554082203875 1104 78-79 0.5257425963065856 1104 80-81 0.7790535690034659 1104 82-83 0.6137873425266704 1104 84-85 0.3997535133124046 1104 86-87 0.6871305056399777 1104 88-89 0.7687520060025328 1104 90-91 0.7133282502467999 1104 92-93 0.6604103940799142 1104 94-95 0.7024982684577381 1104 96-97 0.9794020779331554 1104 98-99 0.7395799311084268 1104 100-101 0.46581745732330404 1105 1 0.25311342861522235 1105 2 0.3020528933491633 1105 3 0.2532624393041658 1105 4 0.1594926906084666 1105 5 0.21981777829510918 1105 6 0.2757773342021892 1105 7 0.24960474218508466 1105 8 0.25770571669887943 1105 9 0.3519226624849239 1105 10-11 0.24164245684320917 1105 12-13 0.2641960167198434 1105 14-15 0.36231804752303987 1105 16-17 0.3682895437149796 1105 18-19 0.41201524049800753 1105 20-21 0.2372294073972725 1105 22-23 0.15706884846265723 1105 24-25 0.22037717641432408 1105 26-27 0.4297861081561294 1105 28-29 0.17628934727204637 1105 30-31 0.33882490313362723 1105 32-33 0.35738527360733485 1105 34-35 0.17300828484208353 1105 36-37 0.42517367375992166 1105 38-39 0.22574796997200508 1105 40-41 0.44365126716488135 1105 42-43 0.3765992452059095 1105 44-45 0.16994291431796427 1105 46-47 0.35283887592223806 1105 48-49 0.14168317153730925 1105 50-51 0.3249266829502062 1105 52-53 0.3841748246492642 1105 54-55 0.4608738650159907 1105 56-57 0.46164349106147995 1105 58-59 0.5139223514989837 1105 60-61 0.3123243097892434 1105 62-63 0.33957303579236253 1105 64-65 0.09036046422762567 1105 66-67 0.33816507254086403 1105 68-69 0.2573153609405239 1105 70-71 0.22884373164930238 1105 72-73 0.363409993862593 1105 74-75 0.5347156207822579 1105 76-77 0.27587758547116437 1105 78-79 0.3256295309686621 1105 80-81 0.3450120943366599 1105 82-83 0.48273760546200606 1105 84-85 0.47566322641585046 1105 86-87 0.6470893778986166 1105 88-89 0.9114107131570179 1105 90-91 1.1111762339898341 1105 92-93 0.9531938955282833 1105 94-95 0.976365345682801 1105 96-97 0.9701222717799922 1105 98-99 0.9083501702982382 1105 100-101 0.6268618646784851 1106 1 0.07653074648863978 1106 2 0.20231644959114092 1106 3 0.06967208100598654 1106 4 0.11760031068791932 1106 5 0.13049295867361366 1106 6 0.023224373228494244 1106 7 0.1413853220088228 1106 8 0.046741623355401885 1106 9 0.1805457924265994 1106 10-11 0.15509919289509355 1106 12-13 0.27644024038151116 1106 14-15 0.31951513073151006 1106 16-17 0.23218733306975992 1106 18-19 0.11013000656302552 1106 20-21 0.08053446414254495 1106 22-23 0.09048824097705221 1106 24-25 0.19955008621354153 1106 26-27 0.20711758199644237 1106 28-29 0.33928313103299246 1106 30-31 0.3629579721423326 1106 32-33 0.3880765132437318 1106 34-35 0.2614994061471094 1106 36-37 0.46229822137292587 1106 38-39 0.5282133349935592 1106 40-41 0.3193687471676441 1106 42-43 0.329322403577855 1106 44-45 0.43593685943987026 1106 46-47 0.4989108890155336 1106 48-49 0.6811839514486309 1106 50-51 0.5961302820681027 1106 52-53 0.61146711082678 1106 54-55 0.5397947443564064 1106 56-57 0.6438545973061522 1106 58-59 0.45618778260169535 1106 60-61 0.4628568009720162 1106 62-63 0.5985804353451663 1106 64-65 0.48409850622757844 1106 66-67 0.24447774398688793 1106 68-69 0.45955443641903315 1106 70-71 0.16560175935728338 1106 72-73 0.2461332290730951 1106 74-75 0.26510065877891975 1106 76-77 0.045700011571685906 1106 78-79 -0.13569570358102467 1106 80-81 0.08692867840188256 1106 82-83 0.22333256638960108 1106 84-85 0.37736558459108593 1106 86-87 0.33303675885648687 1106 88-89 0.24608295830273974 1106 90-91 0.41888006746532014 1106 92-93 0.4150512370679742 1106 94-95 0.7946325594399752 1106 96-97 0.8652703012552614 1106 98-99 0.9216355563735554 1106 100-101 0.4746139527662052 1107 1 0.0913851783499986 1107 2 0.2676412472873686 1107 3 0.10291707002470574 1107 4 0.07238002043391134 1107 5 0.0371550140258563 1107 6 0.12410628711219829 1107 7 0.04092866071810164 1107 8 0.02146762069919106 1107 9 0.19794056446650643 1107 10-11 0.18410341954120213 1107 12-13 0.2214717843601619 1107 14-15 0.18038254014917499 1107 16-17 0.2752175430610251 1107 18-19 0.14489249446127417 1107 20-21 0.18069262516917206 1107 22-23 0.18149170769736145 1107 24-25 0.20145872337015192 1107 26-27 0.17893356937649685 1107 28-29 0.18017219715947164 1107 30-31 0.25970717192635107 1107 32-33 0.24713321711853098 1107 34-35 0.20640390370793682 1107 36-37 0.24156418716685835 1107 38-39 0.30192601951966225 1107 40-41 0.24268535272263136 1107 42-43 0.2514026592705392 1107 44-45 0.24102883281188525 1107 46-47 0.2987939592569049 1107 48-49 0.1680243540916706 1107 50-51 0.07767355490145889 1107 52-53 0.21883339153113468 1107 54-55 0.22188578723104513 1107 56-57 0.26811452251345713 1107 58-59 0.10766652056022252 1107 60-61 0.0998857734370624 1107 62-63 -0.1818857073726683 1107 64-65 -0.08707777891163815 1107 66-67 -3.8922782645300913E-4 1107 68-69 -0.036971785138153024 1107 70-71 0.03316586780316655 1107 72-73 0.2382142964512184 1107 74-75 0.06948623054073622 1107 76-77 -0.06817445715666182 1107 78-79 0.05456307115038328 1107 80-81 0.29165045262863387 1107 82-83 0.200988865833299 1107 84-85 0.1737081182025122 1107 86-87 0.43295464922718807 1107 88-89 0.5258032914937374 1107 90-91 0.5027945205246098 1107 92-93 0.3475310655082531 1107 94-95 0.5438376370618059 1107 96-97 0.6474165740309807 1107 98-99 0.7570189836388934 1107 100-101 0.46030182089202043 1108 1 -0.004470550221185476 1108 2 0.17013894390302298 1108 3 -0.07225758493742163 1108 4 -0.03979729288919742 1108 5 0.012291969236557065 1108 6 -0.12137722981658072 1108 7 0.013495044871518758 1108 8 0.01424623918533996 1108 9 -0.12504148587147057 1108 10-11 -0.06397562544540847 1108 12-13 -0.12671610251005205 1108 14-15 -0.05063810134556235 1108 16-17 -0.013389321934525356 1108 18-19 0.06725581671369696 1108 20-21 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-1.8471368413262539 2316 100-101 -1.5115723960962022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 49.0 5 102.0 6 241.0 7 502.0 8 1216.0 9 2144.0 10 3313.0 11 5210.0 12 7141.0 13 9839.0 14 12371.0 15 16396.0 16 19791.0 17 24269.0 18 21661.0 19 16545.0 20 15966.0 21 16565.0 22 18301.0 23 20314.0 24 23656.0 25 28190.0 26 33902.0 27 40083.0 28 48549.0 29 57940.0 30 68545.0 31 81053.0 32 96744.0 33 118079.0 34 147053.0 35 184410.0 36 228902.0 37 231727.0 38 88529.0 39 2363.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.045154245573876 20.402946276391656 15.357042987518113 22.194856490516354 2 16.117942857683364 21.470667696897074 38.59137077594203 23.820018669477534 3 19.394902086434453 25.431220493806432 30.565726764223566 24.60815065553555 4 13.206074922148211 17.745596674540515 36.56172931459689 32.486599088714385 5 13.117656621787388 38.15828443905828 33.908044278261386 14.816014660892954 6 31.042059397793555 37.11561117682492 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21.21244812468578 26.57783565593067 30.876648106183424 21.33306811320013 30-31 21.329573734830078 26.239987704457725 30.871239662581946 21.559198898130248 32-33 21.181572635970948 26.451073041801425 30.64903910101307 21.71831522121456 34-35 22.008122793032523 26.34011158865008 30.33222702322778 21.319538595089615 36-37 22.040302155326184 26.03735473102684 30.33290347599218 21.589439637654795 38-39 21.6681726591571 26.358918061889643 30.424949994842088 21.547959284111172 40-41 21.748251810258274 26.27842701789968 30.513215129234073 21.460106042607975 42-43 21.72428388091411 26.482144978905247 30.019749913250532 21.77382122693011 44-45 21.764119935208505 26.624043793376607 29.799713876967093 21.812122394447794 46-47 22.17004526272419 26.960108862260512 29.096447966783295 21.773397908232 48-49 22.32555073839675 27.17582012227202 28.607450293738967 21.89117884559226 50-51 22.191699925428253 27.27138646991312 28.538662774630474 21.998250830028145 52-53 22.471184479919653 27.374219445735903 28.332505254434015 21.822090819910425 54-55 22.083861778537443 27.26357551222116 28.47923347046554 22.173329238775853 56-57 22.223867736152187 27.587080449373104 28.149698412768792 22.039353401705917 58-59 22.466646291973298 27.10255899449115 28.23155149349064 22.199243220044913 60-61 22.12620346524689 27.306339375943434 28.701181201158292 21.866275957651382 62-63 22.106626800876764 27.283652425168885 28.687615122517553 21.922105651436798 64-65 21.987825297901868 27.265716789650174 28.556518963302985 22.189938949144977 66-67 21.871932568149635 27.60504674836337 28.185303992194665 22.33771669129233 68-69 22.005014635132536 27.330943751021564 28.48608916095749 22.177952452888412 70-71 21.98165695147389 27.39645937605023 28.358421595719307 22.263462076756575 72-73 22.139911759689838 27.34116581605735 28.26503778455165 22.253884639701162 74-75 21.991738898590754 27.80941886160675 27.982840508837498 22.216001730965 76-77 22.108865051600386 27.63263054974599 28.02963844852434 22.228865950129283 78-79 22.116188668325304 27.548335092567438 28.04302916170697 22.29244707740029 80-81 21.991255187669417 27.90728950128914 27.85588941799677 22.24556589304467 82-83 22.10633126599605 27.820144907702844 27.826411058929313 22.247112767371796 84-85 21.92997882830652 27.84959969449864 27.65934115628836 22.56108032090648 86-87 22.08177557455917 27.718051991926306 27.683441151077947 22.51673128243658 88-89 22.011739491931056 27.798459542674696 27.53953928154357 22.650261683850676 90-91 21.964956380217185 27.78471093654396 27.7449544766365 22.50537820660235 92-93 22.03859688739737 27.64384126873405 27.774602027974055 22.542959815894527 94-95 21.822246117077764 27.44476539609812 27.80071532842832 22.932273158395798 96-97 21.96838461727168 27.27137842252937 27.707927609090742 23.052309351108207 98-99 21.717529680764784 27.5189044185975 27.43020498111923 23.333360919518487 100-101 21.596702612992992 27.52916174664564 27.580206810836216 23.29392882952515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 726.0 1 528.5 2 443.5 3 539.5 4 437.0 5 413.0 6 757.0 7 1526.5 8 2379.0 9 2860.0 10 2626.5 11 2156.0 12 2129.0 13 2257.5 14 2440.0 15 2695.5 16 2902.0 17 3098.0 18 3323.5 19 3632.0 20 4124.5 21 4747.5 22 5404.0 23 6327.0 24 7477.0 25 8936.5 26 11014.5 27 13487.0 28 15592.0 29 18181.0 30 21803.0 31 25760.5 32 30579.5 33 34262.5 34 37362.0 35 39915.5 36 43316.0 37 47641.0 38 49346.5 39 49700.0 40 49955.5 41 51426.0 42 53037.0 43 54768.5 44 56325.0 45 58046.0 46 58469.0 47 59252.5 48 64446.0 49 63913.5 50 58434.0 51 54693.0 52 52009.0 53 51759.0 54 51424.5 55 48435.0 56 44180.0 57 39882.5 58 36474.0 59 32859.0 60 27926.5 61 23512.5 62 19861.0 63 15586.5 64 11888.5 65 9705.5 66 7468.5 67 5923.0 68 4425.0 69 2935.0 70 2270.0 71 1794.5 72 1419.0 73 1199.5 74 1006.5 75 872.5 76 627.5 77 367.0 78 213.0 79 143.0 80 101.5 81 70.0 82 40.0 83 19.0 84 13.0 85 8.5 86 4.5 87 2.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01111321947012406 2 0.0087487046892466 3 0.005024593909364602 4 1.7733860856580948E-4 5 0.0061477384302813945 6 0.0 7 4.1379008665355546E-4 8 0.0016551603466142218 9 5.911286952193649E-5 10-11 0.0 12-13 0.0 14-15 5.024593909364602E-4 16-17 0.004226570170818459 18-19 0.001921168259462936 20-21 6.797979995022697E-4 22-23 5.320158256974284E-4 24-25 4.729029561754919E-4 26-27 8.866930428290474E-5 28-29 1.4778217380484124E-4 30-31 5.320158256974284E-4 32-33 0.005083706778886538 34-35 0.0011527009556777615 36-37 0.001921168259462936 38-39 0.006118181995520426 40-41 2.9556434760968244E-5 42-43 0.0015369346075703486 44-45 0.005852174082671713 46-47 0.0 48-49 0.002423627650399396 50-51 0.0011527009556777615 52-53 7.389108690242061E-4 54-55 1.4778217380484124E-4 56-57 0.012502371903889569 58-59 0.0022462890418335863 60-61 0.003162538519423602 62-63 1.7733860856580948E-4 64-65 0.003339877127989412 66-67 9.162494775900157E-4 68-69 0.001802942520419063 70-71 0.004995037474603633 72-73 0.00286697417181392 74-75 0.0080984631245053 76-77 0.0014778217380484121 78-79 0.007596003733568838 80-81 0.002807861302291983 82-83 0.002985199910857793 84-85 0.0019802811289848727 86-87 7.389108690242061E-4 88-89 0.002275845476594555 90-91 0.007684673037851744 92-93 0.0023645147808774598 94-95 5.024593909364602E-4 96-97 1.4778217380484124E-4 98-99 6.502415647413013E-4 100-101 0.002157619737550682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1691679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.59325180269568 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10100693381688 40.549926719116165 2 13.11933382214471 13.799768543833574 3 4.144030766781988 6.538441607864494 4 1.806797124698252 3.801013445425669 5 0.9773348746923944 2.5700609580126565 6 0.581402508537885 1.8346709118151145 7 0.3679145015917281 1.3544874016853927 8 0.26234286064470896 1.1037971302821346 9 0.20115815108007265 0.9521605162727065 >10 1.2283920105921964 12.527746366128426 >50 0.1314921509996891 4.745534116700662 >100 0.07360925614665437 6.866843103842638 >500 0.003945137816261745 1.38530595931927 >1k 0.0010144640098958776 0.9587706812765557 >5k 2.2543644664352834E-4 1.0114725384245715 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8751 0.5172967211864663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8311 0.4912870585968142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4839 0.28604717561665066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.1420482254612134 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2095 0.12384146164845695 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 1.7733860856580948E-4 0.0 0.0 0.0 12-13 0.0 1.7733860856580948E-4 0.0 0.0 0.0 14-15 0.0 1.7733860856580948E-4 0.0 0.0 0.0 16-17 0.0 1.7733860856580948E-4 0.0 0.0 0.0 18-19 2.9556434760968244E-5 1.7733860856580948E-4 0.0 5.911286952193649E-5 0.0 20-21 5.911286952193649E-5 1.7733860856580948E-4 0.0 5.911286952193649E-5 0.0 22-23 5.911286952193649E-5 1.7733860856580948E-4 0.0 1.7733860856580946E-4 0.0 24-25 1.7733860856580948E-4 2.0689504332677773E-4 0.0 3.5467721713161897E-4 0.0 26-27 1.7733860856580948E-4 2.3645147808774595E-4 0.0 5.320158256974284E-4 0.0 28-29 1.7733860856580948E-4 2.3645147808774595E-4 0.0 0.0012413702599606664 0.0 30-31 1.7733860856580948E-4 2.3645147808774595E-4 0.0 0.002985199910857793 0.0 32-33 1.7733860856580948E-4 2.3645147808774595E-4 0.0 0.0065319720821739825 0.0 34-35 1.7733860856580948E-4 2.3645147808774595E-4 0.0 0.010906324426797284 0.0 36-37 2.3645147808774595E-4 2.3645147808774595E-4 0.0 0.015487571814747362 0.0 38-39 2.9556434760968247E-4 2.9556434760968247E-4 0.0 0.022492446853096836 0.0 40-41 2.9556434760968247E-4 2.9556434760968247E-4 0.0 0.033635222757981864 0.0 42-43 3.251207823706507E-4 2.9556434760968247E-4 0.0 0.046521828313764016 0.0 44-45 4.137900866535554E-4 2.9556434760968247E-4 0.0 0.060620247694745874 0.0 46-47 5.320158256974284E-4 3.251207823706507E-4 0.0 0.07761519768230261 0.0 48-49 5.911286952193649E-4 3.5467721713161897E-4 0.0 0.09461014766985935 0.0 50-51 5.911286952193649E-4 3.5467721713161897E-4 0.0 0.11692525591439037 0.0 52-53 5.911286952193649E-4 3.5467721713161897E-4 0.0 0.1546688231041468 0.0 54-55 5.911286952193649E-4 3.842336518925872E-4 0.0 0.20074730489649634 0.0 56-57 5.911286952193649E-4 5.024593909364602E-4 0.0 0.2456139728636461 0.0 58-59 5.911286952193649E-4 6.502415647413014E-4 0.0 0.306263776993153 0.0 60-61 5.911286952193649E-4 6.502415647413014E-4 0.0 0.3797115173741591 0.0 62-63 5.911286952193649E-4 6.502415647413014E-4 0.0 0.4398588621127294 0.0 64-65 5.911286952193649E-4 6.797979995022697E-4 0.0 0.49619342676713485 0.0 66-67 5.911286952193649E-4 7.093544342632379E-4 0.0 0.5867543428747417 0.0 68-69 5.911286952193649E-4 7.093544342632379E-4 0.0 0.6927732743623347 0.0 70-71 5.911286952193649E-4 7.093544342632379E-4 0.0 0.7784278222996207 0.0 72-73 5.911286952193649E-4 7.093544342632379E-4 0.0 0.8871068329157009 0.0 74-75 5.911286952193649E-4 7.684673037851744E-4 0.0 1.0086428926528024 0.0 76-77 6.206851299803332E-4 7.684673037851744E-4 0.0 1.1405532609910036 0.0 78-79 6.502415647413014E-4 7.684673037851744E-4 0.0 1.2877738625353863 0.0 80-81 7.093544342632379E-4 7.684673037851744E-4 0.0 1.4620090454512942 0.0 82-83 8.275801733071108E-4 7.684673037851744E-4 0.0 1.6517613566167104 0.0 84-85 8.571366080680791E-4 7.684673037851744E-4 0.0 1.8413067727387997 0.0 86-87 9.458059123509839E-4 7.684673037851744E-4 0.0 2.0571869722329117 0.0 88-89 0.0010049187818729204 7.684673037851744E-4 0.0 2.3000226402290265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8555 0.0 35.206444 1 TCGCATA 65 0.0058485405 29.226986 9 GTATCAA 12205 0.0 28.21975 1 GTACATG 9125 0.0 23.844355 1 ATCAACG 15030 0.0 22.942497 3 TCAACGC 15160 0.0 22.77709 4 TATCAAC 15135 0.0 22.753296 2 GTACCTG 1430 0.0 22.590498 1 GAGTACT 9230 0.0 22.437454 12-13 CAACGCA 15475 0.0 22.313456 5 TACATGG 9560 0.0 22.308851 2 ACATGGG 9495 0.0 21.859966 3 AACGCAG 15870 0.0 21.816 6 GTACTTT 9560 0.0 21.688421 14-15 TACCTGG 1425 0.0 21.333094 2 GGACCGT 165 6.3327156E-5 20.14831 6 AGTACTT 9365 0.0 19.856958 12-13 GTATAGG 650 0.0 19.733465 1 TGGACCG 170 7.960983E-5 19.557447 5 GTAAGGT 805 0.0 19.47068 4 >>END_MODULE