FastQCFastQC Report
Thu 26 May 2016
SRR936714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676971
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88690.5288702070578442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74230.44264331345026237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.2571302664148635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.11204725663115224No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17910.10679970017370603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74600.035.2295651
GTATCAA114100.031.9470421
ATCAACG135250.026.7739683
TCAACGC135550.026.6095354
GTACATG90600.026.3327661
TATCAAC138250.026.262512
CAACGCA137950.026.1465935
AACGCAG141050.025.7396346
TACATGG93500.024.8036822
GAGTACT78000.024.00257312-13
ACATGGG93700.023.9385263
ACGCAGA155050.023.322877
CGCAGAG155850.023.0811888
GTACCTG14300.022.599241
GTACTTT83150.022.37073914-15
CATGGGA51600.021.7348864
GCAGAGT161400.021.6993169
AGTACTT80850.021.09942412-13
TACCTGG14750.020.942542
GTGTAAG7750.020.849621
Produced by FastQC (version 0.11.5)