##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936714_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1676971 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.333045711583562 31.0 31.0 34.0 30.0 34.0 2 31.373259883444614 31.0 31.0 34.0 28.0 34.0 3 31.397936517685757 31.0 31.0 34.0 28.0 34.0 4 35.07511877068834 37.0 35.0 37.0 32.0 37.0 5 35.00091593712712 37.0 35.0 37.0 32.0 37.0 6 35.13510430412929 37.0 35.0 37.0 32.0 37.0 7 34.98312791336284 37.0 35.0 37.0 32.0 37.0 8 34.96410253963843 37.0 35.0 37.0 32.0 37.0 9 36.46577668904233 39.0 37.0 39.0 32.0 39.0 10-11 36.33580246766343 39.0 35.0 39.0 32.0 39.0 12-13 36.22789004699544 39.0 35.0 39.0 32.0 39.0 14-15 37.257797839080105 39.0 36.5 41.0 32.0 41.0 16-17 37.188503557902905 39.0 36.0 41.0 31.0 41.0 18-19 37.17157869754456 39.0 36.0 41.0 31.0 41.0 20-21 37.093327791595684 39.0 36.0 41.0 31.0 41.0 22-23 36.981463901283924 39.0 36.0 41.0 31.0 41.0 24-25 36.82790578966482 39.0 36.0 41.0 30.5 41.0 26-27 36.6229404086296 39.0 36.0 40.0 30.0 41.0 28-29 36.726753175815205 39.0 36.0 41.0 30.0 41.0 30-31 36.62784508497762 39.0 36.0 40.5 30.0 41.0 32-33 36.446964199142386 39.0 35.0 40.0 30.0 41.0 34-35 36.265694517078714 39.0 35.0 40.0 30.0 41.0 36-37 36.01663236871717 38.0 35.0 40.0 29.5 41.0 38-39 35.8231633105164 38.0 35.0 40.0 28.5 41.0 40-41 35.64949006273812 38.0 35.0 40.0 27.5 41.0 42-43 35.360747442859775 38.0 34.5 40.0 27.0 41.0 44-45 35.05506326585254 38.0 34.0 40.0 25.5 41.0 46-47 34.648138220637094 38.0 33.5 40.0 24.0 41.0 48-49 34.32980743256741 38.0 33.0 40.0 23.0 41.0 50-51 33.59346583810931 37.5 32.5 39.5 22.5 40.5 52-53 33.77146653102528 37.5 33.0 39.5 23.0 40.5 54-55 34.128421123561466 38.0 33.0 40.0 23.0 41.0 56-57 34.05617866975636 38.0 33.0 40.0 22.5 41.0 58-59 33.81885107136617 38.0 33.0 40.0 22.0 41.0 60-61 33.50602962126358 37.0 33.0 40.0 20.5 41.0 62-63 33.18042649515108 37.0 32.5 40.0 20.0 41.0 64-65 32.872109893373235 36.0 32.0 39.0 19.0 41.0 66-67 32.51022110698396 36.0 32.0 39.0 18.0 41.0 68-69 32.13350946438549 35.0 31.0 39.0 16.0 40.0 70-71 31.742373302817995 35.0 31.0 38.0 13.0 40.0 72-73 31.335622977380048 35.0 31.0 37.5 9.5 39.5 74-75 30.911166919404096 35.0 30.5 37.0 7.5 39.0 76-77 30.496623972626836 35.0 30.0 36.0 7.0 39.0 78-79 30.055441030286154 34.0 29.5 36.0 3.5 38.5 80-81 29.65235236626036 34.0 29.0 35.5 2.0 37.0 82-83 29.24416820565174 34.0 29.0 35.0 2.0 37.0 84-85 28.855633758723314 34.0 29.0 35.0 2.0 36.5 86-87 28.50718945050332 34.0 28.5 35.0 2.0 36.0 88-89 28.125950597833835 34.0 27.5 35.0 2.0 36.0 90-91 27.774642495308505 34.0 27.0 35.0 2.0 35.0 92-93 27.40059190051587 33.0 26.0 35.0 2.0 35.0 94-95 26.969996797797933 33.0 25.5 35.0 2.0 35.0 96-97 26.380299361169634 33.0 25.0 35.0 2.0 35.0 98-99 25.71216556517674 33.0 23.5 35.0 2.0 35.0 100-101 23.60042690064408 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3200255396625806 1101 2 0.46062656273935687 1101 3 0.40207144207066037 1101 4 0.41858244875167117 1101 5 0.4239896942648329 1101 6 0.3370997004565055 1101 7 0.33956216178956566 1101 8 0.3241087378539049 1101 9 0.5702754759863211 1101 10-11 0.48738981129033476 1101 12-13 0.561884126219006 1101 14-15 0.743292339957371 1101 16-17 0.6277485239049483 1101 18-19 0.8390199783401044 1101 20-21 0.8297096791283067 1101 22-23 0.7456078623064641 1101 24-25 0.7103435748963207 1101 26-27 0.6668670162076893 1101 28-29 0.7454470625705909 1101 30-31 0.49568437410083277 1101 32-33 0.4673830249928699 1101 34-35 0.5751992775408326 1101 36-37 0.6134973805397763 1101 38-39 0.6355489844301374 1101 40-41 0.5133438931069634 1101 42-43 0.39890062457558884 1101 44-45 0.5597242801778677 1101 46-47 0.49497732012139295 1101 48-49 0.5255442476388197 1101 50-51 0.5096345025463691 1101 52-53 0.46687707967229386 1101 54-55 0.5271617970921696 1101 56-57 0.5154941317159683 1101 58-59 0.5001465568875361 1101 60-61 0.442502324120305 1101 62-63 0.5895478905816987 1101 64-65 0.6008100330132962 1101 66-67 0.7183749658072358 1101 68-69 0.6336037859022738 1101 70-71 0.5208832643453825 1101 72-73 0.5247872245313339 1101 74-75 0.5374197052100165 1101 76-77 0.5752834684211372 1101 78-79 0.5524840155222108 1101 80-81 0.38966029234969923 1101 82-83 0.5243969910124768 1101 84-85 0.4725750141505216 1101 86-87 0.40592029734913027 1101 88-89 0.5526414446987395 1101 90-91 0.562120427998817 1101 92-93 0.21611038133620397 1101 94-95 0.15593720080937956 1101 96-97 0.024484815896613554 1101 98-99 -0.00784700795558635 1101 100-101 0.4175950572759852 1102 1 0.1747498228617772 1102 2 0.2829432755160184 1102 3 0.2351778932779034 1102 4 0.36729283607561314 1102 5 0.2519408386310289 1102 6 0.3242268382834652 1102 7 0.03630637708131701 1102 8 0.16065583292432706 1102 9 0.3290147868515092 1102 10-11 0.3100833160690115 1102 12-13 0.2616964375872186 1102 14-15 0.304525991868843 1102 16-17 0.2654318111282876 1102 18-19 0.36567255002018584 1102 20-21 0.15089224326312234 1102 22-23 -0.11742250741184534 1102 24-25 0.0499804586991317 1102 26-27 0.07373462812921616 1102 28-29 0.10674516367723186 1102 30-31 -0.0035126832432297306 1102 32-33 -0.018332675459845404 1102 34-35 -0.053561254400818825 1102 36-37 -0.12140830356484855 1102 38-39 -0.1393269901674401 1102 40-41 -0.3573637904946452 1102 42-43 -0.2335545753741073 1102 44-45 -0.12390892530502384 1102 46-47 0.09780192399463061 1102 48-49 0.15048152756840238 1102 50-51 -0.011734335481797586 1102 52-53 -0.04350034110235157 1102 54-55 -0.12332864556376677 1102 56-57 0.012550061041920912 1102 58-59 -0.09717691519496441 1102 60-61 -0.4896996376503182 1102 62-63 -0.213542212537007 1102 64-65 0.008158970387547981 1102 66-67 -0.02024975124508188 1102 68-69 0.09544436060046024 1102 70-71 0.16252357495906367 1102 72-73 0.1340968321772067 1102 74-75 0.05000537548164985 1102 76-77 0.0331165607249595 1102 78-79 0.004233260995047061 1102 80-81 0.027549659804428472 1102 82-83 -0.04677739907806355 1102 84-85 -0.10287864530621604 1102 86-87 -0.10074736487421276 1102 88-89 -0.45911078744411427 1102 90-91 -0.6105795216993748 1102 92-93 -0.4924077587543394 1102 94-95 -0.33544892846627405 1102 96-97 -0.5542721006023754 1102 98-99 -0.5931252398469375 1102 100-101 -0.2397763587199897 1103 1 0.37582536655691 1103 2 0.2685422130658992 1103 3 0.28054339108317095 1103 4 0.265520838868909 1103 5 0.4141746025975124 1103 6 0.1900401363680615 1103 7 0.3708958623167504 1103 8 0.13503170582935553 1103 9 0.5013274863726593 1103 10-11 0.4177053357218412 1103 12-13 0.41469237234379364 1103 14-15 0.6338431307355563 1103 16-17 0.6298328735784153 1103 18-19 0.49023329174005426 1103 20-21 0.4209481715352652 1103 22-23 0.538796940912178 1103 24-25 0.4165525606544449 1103 26-27 0.5230484624268996 1103 28-29 0.5380593845471395 1103 30-31 0.4457528295261213 1103 32-33 0.4119923569623438 1103 34-35 0.38742132490883563 1103 36-37 0.6275866086458421 1103 38-39 0.47703218531140834 1103 40-41 0.3987982954989704 1103 42-43 0.5246516671438499 1103 44-45 0.5994528190086186 1103 46-47 0.8080869274862508 1103 48-49 0.7169117824566698 1103 50-51 0.32519827625004183 1103 52-53 0.5899646554060283 1103 54-55 0.6749704366389508 1103 56-57 0.6486312786197388 1103 58-59 0.6893494839470975 1103 60-61 0.9270376927567057 1103 62-63 0.7436536424111182 1103 64-65 0.6602592422351066 1103 66-67 0.6311179038826182 1103 68-69 0.7040695975318201 1103 70-71 0.6970348104937791 1103 72-73 0.5792808904373814 1103 74-75 0.38682170379370007 1103 76-77 0.5085583130194529 1103 78-79 0.5559516368928925 1103 80-81 0.6037078177254145 1103 82-83 0.5380786339426855 1103 84-85 0.33512202807765945 1103 86-87 0.42484153426784843 1103 88-89 0.444429154195948 1103 90-91 0.7158445605033386 1103 92-93 0.6088981695178113 1103 94-95 0.5283834987085108 1103 96-97 0.6999864289267492 1103 98-99 0.7737573668968771 1103 100-101 0.7011098892847976 1104 1 0.1911669132906617 1104 2 0.27123191671753233 1104 3 0.19597126484838867 1104 4 0.3903164287908467 1104 5 0.3308677839952665 1104 6 0.29715286713872047 1104 7 0.41315417745974514 1104 8 0.3552871105181481 1104 9 0.49325467568784376 1104 10-11 0.5857819077987543 1104 12-13 0.5574319606871825 1104 14-15 0.44648140814130954 1104 16-17 0.33398643703494457 1104 18-19 0.490872728394514 1104 20-21 0.5300307001773419 1104 22-23 0.5573412205131021 1104 24-25 0.46353419766160897 1104 26-27 0.43150635333419984 1104 28-29 0.4800759840020419 1104 30-31 0.5305090173244125 1104 32-33 0.4094719159545406 1104 34-35 0.3997565327720025 1104 36-37 0.357339528349371 1104 38-39 0.43306305339137197 1104 40-41 0.1777899957715121 1104 42-43 0.3708098059330496 1104 44-45 0.2901775960998876 1104 46-47 0.4926134237186872 1104 48-49 0.20561311559268347 1104 50-51 0.20979229255352294 1104 52-53 0.3990915808071449 1104 54-55 0.5071605534270915 1104 56-57 0.5024210353008343 1104 58-59 0.41726980409706016 1104 60-61 0.31295035446573394 1104 62-63 0.2026797501719102 1104 64-65 0.33868056747837016 1104 66-67 0.207961602625943 1104 68-69 0.1614533335190984 1104 70-71 0.30670066321986766 1104 72-73 -0.11799734158501352 1104 74-75 -0.2033126580645508 1104 76-77 -0.19034225057274057 1104 78-79 -0.06213480113046188 1104 80-81 0.009210241048513979 1104 82-83 -0.2774192263801787 1104 84-85 -0.11982790224409356 1104 86-87 -0.21114543035040256 1104 88-89 -0.06444464801649374 1104 90-91 -0.12078430725597755 1104 92-93 -0.2277146660785263 1104 94-95 -0.35454409757696226 1104 96-97 -0.157874572220166 1104 98-99 -0.20636953383936785 1104 100-101 0.11432748273380966 1105 1 0.18892007317032267 1105 2 0.16703455714205973 1105 3 0.16275498175368242 1105 4 0.3285003144773384 1105 5 0.315141095110512 1105 6 0.29605507416745525 1105 7 0.3033391216046155 1105 8 -0.020221259408288006 1105 9 0.3618484994402209 1105 10-11 0.40927205507703235 1105 12-13 0.2633801868158514 1105 14-15 0.4468962093942963 1105 16-17 0.3883599679471743 1105 18-19 0.17295557898501102 1105 20-21 -0.19306204842926178 1105 22-23 -0.37767324985244954 1105 24-25 -0.15867066171123412 1105 26-27 0.0037276617222445907 1105 28-29 0.20740733391789945 1105 30-31 0.1182162391572632 1105 32-33 -0.11873993305780317 1105 34-35 -0.1209286998266137 1105 36-37 -0.18729749671511797 1105 38-39 -0.1377004908231001 1105 40-41 -0.3116856143915925 1105 42-43 -0.2220401170664914 1105 44-45 -0.07154697586812375 1105 46-47 -0.057330196685718704 1105 48-49 0.22672089716883193 1105 50-51 -0.034564384077910404 1105 52-53 0.10548977093868217 1105 54-55 0.20919796659297418 1105 56-57 0.19664218635046637 1105 58-59 0.139071662597388 1105 60-61 0.25906135432746424 1105 62-63 -0.14411316864622847 1105 64-65 0.015564694893875242 1105 66-67 -0.48998143610314315 1105 68-69 -0.03639971240941264 1105 70-71 0.17048612991894885 1105 72-73 -0.10666122860650162 1105 74-75 0.029467130148415066 1105 76-77 0.09482169127879914 1105 78-79 0.2027879123372287 1105 80-81 0.2906743464366386 1105 82-83 0.28056116830083155 1105 84-85 0.05795496121954713 1105 86-87 0.1292073414278576 1105 88-89 0.21335418767930392 1105 90-91 0.27928375352432155 1105 92-93 0.20163740959647924 1105 94-95 0.25105142829090354 1105 96-97 0.4197111414525452 1105 98-99 0.31638016936318536 1105 100-101 0.40677249772863533 1106 1 0.12056609254225847 1106 2 0.07870129965354877 1106 3 0.077681884846168 1106 4 0.2492865219210998 1106 5 0.29529367155917896 1106 6 0.2795098175610775 1106 7 0.3715317861266243 1106 8 0.26658885899131946 1106 9 0.3827845879242915 1106 10-11 0.4771655701112323 1106 12-13 0.49754174562534104 1106 14-15 0.48532473964996115 1106 16-17 0.48263975133435366 1106 18-19 0.39137218993489853 1106 20-21 0.3827651268878043 1106 22-23 0.42651700163732187 1106 24-25 0.25306536212971054 1106 26-27 0.17934401760015817 1106 28-29 0.1492641085525861 1106 30-31 0.3357820753814451 1106 32-33 0.25878059463547487 1106 34-35 0.19139547145200453 1106 36-37 0.18076547315816072 1106 38-39 0.19327902757392934 1106 40-41 0.26330668444168026 1106 42-43 0.16861687996856034 1106 44-45 0.13453682916166798 1106 46-47 0.274139250102138 1106 48-49 0.1057221491918483 1106 50-51 0.29473429633883796 1106 52-53 0.2126481512666416 1106 54-55 0.37089986026320076 1106 56-57 0.3909840458088425 1106 58-59 0.4415715955038948 1106 60-61 0.45259386200238083 1106 62-63 0.332391271776487 1106 64-65 -0.02096304739242072 1106 66-67 0.0837648197879517 1106 68-69 -0.025547339108193512 1106 70-71 0.18447614523960354 1106 72-73 0.3253749540418376 1106 74-75 0.2768631731489357 1106 76-77 0.03922043435504108 1106 78-79 0.18184598963593857 1106 80-81 0.12882922273373154 1106 82-83 0.21736478154823757 1106 84-85 0.29751273338869666 1106 86-87 0.510964240098005 1106 88-89 0.43612064513093785 1106 90-91 0.3571008344787536 1106 92-93 0.2311419211966559 1106 94-95 0.1868788311424332 1106 96-97 0.21808249054526385 1106 98-99 0.3968876995873458 1106 100-101 0.5693817580315716 1107 1 0.25948232657342274 1107 2 0.27004659331542413 1107 3 0.328207473037061 1107 4 0.39491624831579486 1107 5 0.2975257837811469 1107 6 0.39130195788095534 1107 7 0.6191327957550214 1107 8 0.5572980817695949 1107 9 0.6894027569933812 1107 10-11 0.6000962810558192 1107 12-13 0.49055422998246456 1107 14-15 0.6101439809813414 1107 16-17 0.5984786088023313 1107 18-19 0.8171227984942817 1107 20-21 0.8304553773360297 1107 22-23 0.779508425036191 1107 24-25 0.8796120262510954 1107 26-27 0.8910861880516876 1107 28-29 0.6145821502328204 1107 30-31 0.6391305244439991 1107 32-33 0.6822760087615336 1107 34-35 0.6382773766203016 1107 36-37 0.7492769075865837 1107 38-39 0.7936913257761447 1107 40-41 0.6567262890995451 1107 42-43 0.7116967082369072 1107 44-45 0.774777950932183 1107 46-47 0.8113150230131012 1107 48-49 0.6740408159633233 1107 50-51 0.35909779500318706 1107 52-53 0.5122561903641625 1107 54-55 0.7278034065642416 1107 56-57 0.6367194513009125 1107 58-59 0.6888898480432459 1107 60-61 0.6308515418283989 1107 62-63 0.6738234980370521 1107 64-65 0.43326555134267863 1107 66-67 0.43785826281793305 1107 68-69 0.5505108867057018 1107 70-71 0.607242598177475 1107 72-73 0.607759608488653 1107 74-75 0.4166548156416603 1107 76-77 0.6715242386777796 1107 78-79 0.5165177793058362 1107 80-81 0.5617258031976959 1107 82-83 0.48455572633433874 1107 84-85 0.6682738724272035 1107 86-87 0.6859869791954054 1107 88-89 0.8509901744908852 1107 90-91 0.922026260220786 1107 92-93 1.0322044515310473 1107 94-95 1.0154756323222465 1107 96-97 1.0991245712880868 1107 98-99 1.2273689761708795 1107 100-101 1.6401841019365584 1108 1 0.11170741334950662 1108 2 0.012766543171220945 1108 3 0.023472004844180105 1108 4 0.13309032530555243 1108 5 0.25142968152278655 1108 6 0.057027798934285556 1108 7 -0.020905956559730043 1108 8 0.0425532985947612 1108 9 0.2171185592570808 1108 10-11 0.1578695339992393 1108 12-13 0.2476746258132323 1108 14-15 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-2.1271248028030882 2316 62-63 -1.78136129284254 2316 64-65 -1.705760633761919 2316 66-67 -2.560052535617494 2316 68-69 -2.177972172739757 2316 70-71 -1.6979368528898213 2316 72-73 -1.6813578540325373 2316 74-75 -1.8225737817231398 2316 76-77 -2.383205500071906 2316 78-79 -2.0553894681544165 2316 80-81 -2.15423289335412 2316 82-83 -2.07674198155123 2316 84-85 -2.3782934626059706 2316 86-87 -2.5270172993292057 2316 88-89 -2.815219826977561 2316 90-91 -3.0065203763649393 2316 92-93 -3.12653148140091 2316 94-95 -3.13996546954802 2316 96-97 -3.4917398442027086 2316 98-99 -4.015919547237523 2316 100-101 -4.688820924161774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3668.0 3 1398.0 4 1447.0 5 1579.0 6 1824.0 7 2159.0 8 2728.0 9 3532.0 10 4414.0 11 5747.0 12 7515.0 13 9102.0 14 10524.0 15 12717.0 16 15674.0 17 19402.0 18 16700.0 19 11373.0 20 11050.0 21 11944.0 22 13115.0 23 14785.0 24 16856.0 25 19592.0 26 23016.0 27 27576.0 28 33035.0 29 39527.0 30 48662.0 31 59943.0 32 74400.0 33 95654.0 34 124624.0 35 167863.0 36 225842.0 37 283957.0 38 219757.0 39 34246.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.477464179749816 18.883451864442158 14.207332528216368 22.431751427591657 2 15.748146540208388 20.257695316972637 39.86596408534025 24.128194057478723 3 19.313631825842396 25.148166834245174 29.813230472332847 25.724970867579582 4 12.56974775679761 16.657440536197253 37.25899219636387 33.51381951064127 5 13.51153353752024 37.82013575605432 33.73838622346892 14.929944482956516 6 31.292905427577605 36.17683032331585 17.519852989433616 15.010411259672926 7 28.526898748333647 33.454593171156496 20.39568999710718 17.622818083402674 8 27.034925853314633 33.83054725341449 19.682764437027355 19.451762456243525 9 27.21123934845463 16.438030191703962 20.53082387854614 35.81990658129527 10-11 25.21134456964811 26.68895197790837 26.65151814332953 21.448185309113992 12-13 26.411526296511497 24.33818765786199 27.371106468628042 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21.760969908042412 26.530062139952054 29.961684338585215 21.747283613420322 40-41 21.889394944578207 26.430985516537085 30.014923259263675 21.66469627962103 42-43 21.809458243053427 26.616458765725255 29.569897442426342 22.00418554879498 44-45 21.899586989267444 26.78436617820167 29.38148571755695 21.934561114973935 46-47 22.234052671705424 27.044795130638676 28.784815387840133 21.936336809815767 48-49 22.320581217182127 27.275638378533586 28.33526191834229 22.068518485942 50-51 22.19364518349848 27.3729038190499 28.288018385776308 22.145432611675314 52-53 22.472049141274006 27.428992087472515 28.17912879309656 21.919829978156915 54-55 22.035512429230966 27.375376729197455 28.284267597889958 22.304843243681624 56-57 22.177130088965775 27.747703103932945 27.99058640843395 22.084580398667335 58-59 22.352049142079874 27.24991778147434 28.079527303855517 22.318505772590267 60-61 22.134109817070936 27.359840998138594 28.586017031398754 21.920032153391713 62-63 22.063405975889044 27.433077383389126 28.576160786615983 21.927355854105848 64-65 21.888546128621172 27.43067751440299 28.450649379304423 22.230126977671418 66-67 21.81898252284529 27.754017455686935 28.038525116104886 22.38847490536289 68-69 21.95201222281228 27.476936866395583 28.384016716914218 22.18703419387792 70-71 21.962806602803074 27.600529102106808 28.141469212543157 22.295195082546964 72-73 22.058820021563445 27.563849504327003 28.12297246367132 22.254358010438235 74-75 21.91178443749385 27.958632369559577 27.920079431575246 22.209503761371327 76-77 22.108554600065062 27.67493193608626 27.967701432957455 22.248812030891223 78-79 22.130809924424792 27.760311546790923 27.855097456796802 22.253781071987483 80-81 21.9969837649973 28.099297772536993 27.73042481674896 22.173293645716747 82-83 22.080685736026336 28.003948921634898 27.69373614083255 22.221629201506218 84-85 21.963600538632832 27.947780286467744 27.620646717214186 22.46797245768524 86-87 22.137698434351883 27.920610455763516 27.636714294787446 22.30497681509716 88-89 22.079977148023204 27.933858078012786 27.44670307159908 22.539461702364928 90-91 22.00383413332872 27.979571565866852 27.70147911429075 22.31511518651368 92-93 22.226095513575572 27.78198571620272 27.735585740058745 22.256333030162967 94-95 21.975266971083574 27.53116377148575 27.841236067022606 22.652333190408065 96-97 22.18446753920535 27.414928968950285 27.73325302677697 22.667350465067393 98-99 21.872260096265677 27.68685025140015 27.480183226868505 22.96070642546567 100-101 21.928362740989414 27.484761758463993 27.731616051494594 22.855259449052 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 630.0 1 443.0 2 366.5 3 497.5 4 458.5 5 480.0 6 806.0 7 1478.5 8 2415.5 9 2933.0 10 2600.0 11 2260.0 12 2299.5 13 2405.0 14 2596.0 15 2735.5 16 2858.0 17 2961.5 18 3124.0 19 3340.0 20 3608.0 21 4244.0 22 4994.5 23 5837.5 24 6916.5 25 8335.5 26 10481.0 27 13051.5 28 15122.0 29 17426.0 30 20936.5 31 24701.5 32 29086.0 33 33052.5 34 36419.5 35 39329.0 36 42850.5 37 46572.0 38 47972.5 39 48458.0 40 49026.0 41 50901.5 42 52707.5 43 54320.5 44 55886.5 45 57876.0 46 58306.5 47 58698.5 48 63956.0 49 63669.0 50 58443.0 51 55124.5 52 52352.5 53 52243.5 54 51761.5 55 48406.0 56 44543.0 57 40416.5 58 36904.0 59 33328.5 60 28145.5 61 23422.0 62 20034.5 63 15966.5 64 12148.5 65 9792.0 66 7577.0 67 6088.5 68 4561.0 69 3058.0 70 2284.5 71 1793.5 72 1484.0 73 1307.5 74 1074.5 75 884.5 76 647.5 77 362.0 78 233.0 79 160.0 80 90.5 81 63.0 82 45.0 83 27.5 84 21.5 85 13.0 86 9.5 87 7.5 88 4.0 89 5.5 90 5.0 91 2.0 92 4.0 93 5.0 94 3.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011687739382493794 2 0.00638055160166753 3 0.008646541890110205 4 0.03416874829678033 5 0.0434712347440713 6 0.05372782236544341 7 0.024210317292308572 8 0.021586539063585476 9 0.013059259820235413 10-11 0.040549299898447855 12-13 0.0396548300477468 14-15 0.017889397014021112 16-17 0.02069206921288442 18-19 0.02775838103342276 20-21 0.022779165531186883 22-23 0.012224421292914426 24-25 0.0023256216118227447 26-27 0.002236174626752639 28-29 0.0010733638208412668 30-31 0.005158109472376088 32-33 0.001103179482531302 34-35 0.0013715204377416188 36-37 0.005814054029556861 38-39 0.006619076895187811 40-41 0.0034884324177341173 42-43 6.26128895490739E-4 44-45 0.0010137324974611964 46-47 0.002176543303372569 48-49 4.4723492535052783E-4 50-51 0.0013417047760515837 52-53 5.963132338007037E-4 54-55 0.002206358965062604 56-57 0.002027464994922393 58-59 0.002236174626752639 60-61 5.664975721106685E-4 62-63 9.242855123910908E-4 64-65 0.003697142049564363 66-67 0.0023256216118227447 68-69 0.006350735939977496 70-71 0.010018062327851823 72-73 0.003518248079424152 74-75 0.003339354109283941 76-77 0.0032499071242138356 78-79 0.03348298807790952 80-81 0.022510824575976567 82-83 0.0034288010943540467 84-85 0.00793096600954936 86-87 0.007483731084198832 88-89 0.005486081750966475 90-91 0.011359767103903406 92-93 0.014669305551497314 94-95 0.006201657631527319 96-97 0.003786589034634469 98-99 0.021526907740205405 100-101 0.04045985291337775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1676971.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.17544828473868 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10989751103324 42.31638713135219 2 12.671700502383048 13.729901104931 3 3.929167888342927 6.385932952109344 4 1.7048841467075775 3.6945145168570876 5 0.8866518571160458 2.4017380915878923 6 0.5673636682517487 1.8442308640807341 7 0.3395889504959425 1.2878168537963133 8 0.2592040847932178 1.1233997992726392 9 0.1831810825633043 0.8931525538635663 >10 1.15268653359488 12.066882072575357 >50 0.12393520626835472 4.638299725001503 >100 0.06709625397083105 6.547474758036093 >500 0.0034264702106341196 1.2146226686650397 >1k 9.94781674055067E-4 0.880068495433351 >5k 2.2106259423445933E-4 0.9755784124379229 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8869 0.5288702070578442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7423 0.44264331345026237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 0.2571302664148635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1879 0.11204725663115224 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1791 0.10679970017370603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.1926264676014075E-4 0.0 0.0 0.0 0.0 8 1.1926264676014075E-4 0.0 0.0 0.0 0.0 9 1.1926264676014075E-4 0.0 0.0 0.0 0.0 10-11 1.7889397014021112E-4 0.0 0.0 0.0 0.0 12-13 1.7889397014021112E-4 0.0 0.0 0.0 0.0 14-15 1.7889397014021112E-4 0.0 0.0 0.0 0.0 16-17 1.7889397014021112E-4 5.9631323380070374E-5 0.0 0.0 0.0 18-19 2.385252935202815E-4 1.1926264676014075E-4 0.0 0.0 0.0 20-21 2.385252935202815E-4 1.1926264676014075E-4 0.0 0.0 0.0 22-23 2.385252935202815E-4 1.1926264676014075E-4 0.0 2.385252935202815E-4 0.0 24-25 2.9815661690035187E-4 1.4907830845017594E-4 0.0 4.174192636604926E-4 0.0 26-27 2.9815661690035187E-4 1.7889397014021112E-4 0.0 6.559445571807741E-4 0.0 28-29 2.9815661690035187E-4 1.7889397014021112E-4 0.0 0.001282073452671513 0.0 30-31 2.9815661690035187E-4 1.7889397014021112E-4 0.0 0.0029219348456234486 0.0 32-33 2.9815661690035187E-4 2.385252935202815E-4 0.0 0.006201657631527319 0.0 34-35 2.9815661690035187E-4 2.385252935202815E-4 0.0 0.010524928576582422 0.0 36-37 2.9815661690035187E-4 2.385252935202815E-4 0.0 0.015444512755438228 0.0 38-39 4.174192636604926E-4 2.9815661690035187E-4 0.0 0.02152690774020541 0.0 40-41 4.174192636604926E-4 2.9815661690035187E-4 0.0 0.030024371321865435 0.0 42-43 4.174192636604926E-4 2.9815661690035187E-4 0.0 0.04114561313224856 0.0 44-45 4.77050587040563E-4 2.9815661690035187E-4 0.0 0.054175057290793936 0.0 46-47 4.77050587040563E-4 2.9815661690035187E-4 0.0 0.07033514592679302 0.0 48-49 4.77050587040563E-4 2.9815661690035187E-4 0.0 0.0870319164732127 0.0 50-51 4.77050587040563E-4 2.9815661690035187E-4 0.0 0.10978126634270957 0.0 52-53 4.77050587040563E-4 2.9815661690035187E-4 0.0 0.14281701949526854 0.0 54-55 4.77050587040563E-4 2.9815661690035187E-4 0.0 0.18282963748329578 0.0 56-57 4.77050587040563E-4 3.8760360197045746E-4 0.0 0.22436285421751478 0.0 58-59 4.77050587040563E-4 5.068662487305982E-4 0.0 0.283845099289135 0.0 60-61 5.068662487305982E-4 5.366819104206334E-4 0.0 0.3517949922807252 0.0 62-63 5.366819104206334E-4 5.366819104206334E-4 0.0 0.40999516389967383 0.0 64-65 5.366819104206334E-4 5.963132338007037E-4 0.0 0.4680462572101724 0.0 66-67 5.366819104206334E-4 6.26128895490739E-4 0.0 0.5564496941211268 0.0 68-69 5.366819104206334E-4 6.857602188708094E-4 0.0 0.6543643271112023 0.0 70-71 5.664975721106685E-4 7.453915422508797E-4 0.0 0.7348367980126073 0.0 72-73 5.963132338007037E-4 7.752072039409149E-4 0.0 0.8408314753206824 0.0 74-75 5.963132338007037E-4 7.752072039409149E-4 0.0 0.9606308039912437 0.0 76-77 5.963132338007037E-4 7.752072039409149E-4 0.0 1.0926545539547194 0.0 78-79 5.963132338007037E-4 8.0502286563095E-4 0.0 1.2380953516787112 0.0 80-81 6.559445571807742E-4 8.944698507010556E-4 0.0 1.4045860065558677 0.0 82-83 6.559445571807742E-4 9.54101174081126E-4 0.0 1.5883101138898645 0.0 84-85 6.559445571807742E-4 9.54101174081126E-4 0.0 1.7749561560694849 0.0 86-87 6.559445571807742E-4 0.0010137324974611964 0.0 1.9915967539092803 0.0 88-89 6.559445571807742E-4 0.0010137324974611964 0.0 2.2409749482847348 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7460 0.0 35.229565 1 GTATCAA 11410 0.0 31.947042 1 ATCAACG 13525 0.0 26.773968 3 TCAACGC 13555 0.0 26.609535 4 GTACATG 9060 0.0 26.332766 1 TATCAAC 13825 0.0 26.26251 2 CAACGCA 13795 0.0 26.146593 5 AACGCAG 14105 0.0 25.739634 6 TACATGG 9350 0.0 24.803682 2 GAGTACT 7800 0.0 24.002573 12-13 ACATGGG 9370 0.0 23.938526 3 ACGCAGA 15505 0.0 23.32287 7 CGCAGAG 15585 0.0 23.081188 8 GTACCTG 1430 0.0 22.59924 1 GTACTTT 8315 0.0 22.370739 14-15 CATGGGA 5160 0.0 21.734886 4 GCAGAGT 16140 0.0 21.699316 9 AGTACTT 8085 0.0 21.099424 12-13 TACCTGG 1475 0.0 20.94254 2 GTGTAAG 775 0.0 20.84962 1 >>END_MODULE