FastQCFastQC Report
Thu 26 May 2016
SRR936714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676971
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86950.5184943567897119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82640.49279325641290156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50900.3035234360045582No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.13428974025191848No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20760.1237946273370261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86100.034.534741
GTATCAA122100.027.8482721
TATAACG1056.9080925E-527.1426262
GTACATG90000.023.9260791
GAGTACT90100.023.21637712-13
TACATGG94600.022.243472
ATCAACG151150.022.2164823
TCAACGC151350.022.1537594
GTACTTT93800.022.12340514-15
TATCAAC152750.022.0161782
CAACGCA154350.021.7519955
ACATGGG94000.021.6261863
AACGCAG157850.021.2997746
GTATACC2257.168455E-821.1077844
TACCTGG12250.020.5508462
AGTACTT93250.019.6058312-13
ACTTTTT109350.019.26021816-17
ACGCAGA176150.019.0600097
CGCAGAG177300.018.936388
GTACCTG14000.018.840931
Produced by FastQC (version 0.11.5)