FastQCFastQC Report
Thu 26 May 2016
SRR936713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690354
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84150.49782471600623307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72980.43174388323392615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.2546803805593385No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.11624783921001162No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18120.10719648073717104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1308.260031E-932.8979761
GGTATCA76850.032.2154271
GTATCAA114050.030.8323381
GTACATG94250.026.1167091
TCAACGC138300.025.1556824
ATCAACG138850.025.0195833
CAACGCA140500.024.6933985
TACATGG97450.024.5786082
TATCAAC141600.024.5001182
AACGCAG143200.024.292756
ACATGGG96900.023.7862823
GAGTACT79050.023.26509512-13
GTACTTT83200.021.90472214-15
ACGCAGA160200.021.5968467
CATGGGA52400.021.5870274
CGCAGAG161650.021.3730898
TACCTGG14100.021.2339132
AGTACTT82300.020.6140912-13
GCAGAGT167800.020.3619739
ATGGGAG23500.020.0217325
Produced by FastQC (version 0.11.5)