FastQCFastQC Report
Thu 26 May 2016
SRR936713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690354
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88140.5214292390824644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85160.503799795782422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51770.3062672079339594No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23440.1386691781721462No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20830.12322862548318281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79950.038.455371
GTATCAA116450.029.380841
GAGTACT93800.023.52164612-13
TCAACGC145600.023.4194814
GTACATG88750.023.39831
ATCAACG145900.023.3394683
TATCAAC147450.023.0317462
CAACGCA148250.023.0015345
TACACCG1452.337952E-522.9275235
GTACTTT97400.022.53001614-15
TACATGG92050.022.4455952
AACGCAG152950.022.3264336
AGGTAAG6500.021.9216632
GTAAGGT7000.021.7103184
ACATGGG93850.021.456493
GGTAAGG7200.020.4482563
AGTACTT96600.020.3813212-13
ACGCAGA170850.019.903897
CGCAGAG171800.019.7385378
ACTTTTT115150.019.26190616-17
Produced by FastQC (version 0.11.5)