##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936713_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1690354 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.457674546278472 31.0 31.0 34.0 30.0 34.0 2 31.600152985705954 31.0 31.0 34.0 30.0 34.0 3 31.68343672390517 31.0 31.0 34.0 30.0 34.0 4 35.51331259605976 37.0 35.0 37.0 33.0 37.0 5 35.24477535474818 37.0 35.0 37.0 33.0 37.0 6 35.35700924185112 37.0 35.0 37.0 33.0 37.0 7 35.2848214042739 37.0 35.0 37.0 32.0 37.0 8 35.25639895548506 37.0 35.0 37.0 32.0 37.0 9 36.79783879589719 39.0 37.0 39.0 32.0 39.0 10-11 36.64270176542902 38.5 35.0 39.0 32.0 39.0 12-13 36.517400497173966 38.0 35.0 39.0 32.0 39.0 14-15 37.475518145903166 39.0 36.0 41.0 32.0 41.0 16-17 37.39082937656846 39.0 36.0 40.0 32.0 41.0 18-19 37.576593719422085 39.0 36.5 41.0 32.0 41.0 20-21 37.62827401834172 39.0 37.0 41.0 32.0 41.0 22-23 37.527262040968935 39.0 36.0 41.0 32.0 41.0 24-25 37.3782323111017 39.0 36.0 41.0 32.0 41.0 26-27 37.10657678805741 39.0 36.0 40.5 31.0 41.0 28-29 36.98515015198 39.0 36.0 40.0 31.0 41.0 30-31 36.89630130730013 39.0 36.0 40.0 31.0 41.0 32-33 36.70001816187615 39.0 35.0 40.0 30.0 41.0 34-35 36.491358614822694 38.5 35.0 40.0 30.0 41.0 36-37 36.18111147132494 38.0 35.0 40.0 30.0 41.0 38-39 35.98442988865054 38.0 35.0 40.0 30.0 41.0 40-41 35.71868969458468 38.0 35.0 40.0 28.5 41.0 42-43 35.40543667184507 38.0 34.0 40.0 27.0 41.0 44-45 35.09666170518128 38.0 34.0 40.0 26.5 41.0 46-47 34.66605752404526 38.0 33.5 40.0 24.5 41.0 48-49 34.22928096718202 38.0 33.0 40.0 24.0 41.0 50-51 33.86118085324139 38.0 33.0 40.0 23.0 41.0 52-53 33.53724634011574 37.0 32.5 40.0 22.0 41.0 54-55 33.23680453916754 37.0 32.0 40.0 20.5 41.0 56-57 32.88501935097618 36.5 31.5 39.5 19.5 41.0 58-59 32.5086765849047 36.0 31.0 39.0 18.0 40.0 60-61 32.11576717066366 36.0 31.0 39.0 16.0 40.0 62-63 31.67487431626748 35.0 30.0 39.0 13.0 40.0 64-65 31.219485681697442 35.0 29.5 38.0 10.0 40.0 66-67 30.719611099213537 34.5 29.0 38.0 8.0 40.0 68-69 30.39629213762324 34.0 29.0 37.5 7.5 39.5 70-71 29.928579457320772 34.0 28.5 37.0 6.5 39.0 72-73 29.44766540026527 34.0 28.0 36.0 2.0 39.0 74-75 28.864176675418285 33.5 27.0 36.0 2.0 38.5 76-77 27.559667383281845 31.5 26.0 34.5 2.0 36.5 78-79 28.332023351321673 33.0 27.0 35.0 2.0 37.0 80-81 28.10833115430259 33.0 26.0 35.0 2.0 37.0 82-83 27.718516653907997 33.0 26.0 35.0 2.0 36.0 84-85 27.24007988859138 32.5 26.0 35.0 2.0 36.0 86-87 26.77188151121008 32.0 25.0 35.0 2.0 35.5 88-89 26.273009973058898 32.0 24.5 34.0 2.0 35.0 90-91 25.68202932640145 31.5 23.0 34.0 2.0 35.0 92-93 25.052315372992876 31.0 20.0 34.0 2.0 35.0 94-95 24.29404698660754 31.0 16.0 34.0 2.0 35.0 96-97 23.336294941769594 31.0 3.5 34.0 2.0 35.0 98-99 22.142710047717813 30.0 2.0 34.0 2.0 35.0 100-101 18.885205998270184 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23911726131415278 1101 2 0.40144747583869034 1101 3 0.3040537918792978 1101 4 0.3216319389750524 1101 5 0.07052226007444773 1101 6 0.2190565720060036 1101 7 0.14946479239107902 1101 8 0.22545760865601494 1101 9 0.2833978403232038 1101 10-11 0.2854102508941523 1101 12-13 0.37585779774489936 1101 14-15 0.5544852481577394 1101 16-17 0.6812448939863174 1101 18-19 0.47788707066369085 1101 20-21 0.5585081843664454 1101 22-23 0.45466068263390724 1101 24-25 0.35479174671971947 1101 26-27 0.3732783083699829 1101 28-29 0.3404558341266295 1101 30-31 0.35789148884867217 1101 32-33 0.47672511345102464 1101 34-35 0.36490644239583503 1101 36-37 0.5299488516767212 1101 38-39 0.6522145430919863 1101 40-41 0.6625880211185233 1101 42-43 0.7717084376308421 1101 44-45 0.4515079276761469 1101 46-47 0.6707074728499833 1101 48-49 0.4101454749643665 1101 50-51 0.606937817651712 1101 52-53 0.5278906645343469 1101 54-55 0.5599532166722483 1101 56-57 0.5503153762606274 1101 58-59 0.39449191087347657 1101 60-61 0.6692477052758292 1101 62-63 0.5600554725995437 1101 64-65 0.558351929166939 1101 66-67 0.5805716033009674 1101 68-69 0.4377375978703384 1101 70-71 0.4535114476329838 1101 72-73 0.6011076511253393 1101 74-75 0.5444261038470977 1101 76-77 0.3846774880394399 1101 78-79 0.35041000395349897 1101 80-81 0.5953463738341824 1101 82-83 0.7549883709860765 1101 84-85 0.6279937379615284 1101 86-87 0.5633929829333439 1101 88-89 0.48815986172487413 1101 90-91 0.5072511959757691 1101 92-93 0.48624822190527084 1101 94-95 0.3316040181356392 1101 96-97 0.5393970419294867 1101 98-99 0.9456514429681384 1101 100-101 1.187113087774275 1102 1 0.30664354837506735 1102 2 0.35611246902383087 1102 3 0.28859631367214433 1102 4 0.22079879358153676 1102 5 0.09001309024548476 1102 6 0.20742260686802183 1102 7 0.3140333969624294 1102 8 0.21947262423050518 1102 9 0.2670019704233155 1102 10-11 0.33500528143846253 1102 12-13 0.26846424522470613 1102 14-15 0.41708113832243043 1102 16-17 0.4158393852250555 1102 18-19 0.3002445550810293 1102 20-21 0.08471712768706396 1102 22-23 0.16786419040807488 1102 24-25 0.3034874443686775 1102 26-27 0.04325173531368165 1102 28-29 0.17055774653686484 1102 30-31 0.33761490680394957 1102 32-33 0.292785469915664 1102 34-35 0.22278079400345518 1102 36-37 0.3171591383584911 1102 38-39 0.20737961962435492 1102 40-41 0.15612272788337833 1102 42-43 0.24135305160245224 1102 44-45 0.39369535107024234 1102 46-47 0.44383224201085625 1102 48-49 0.4590469258597736 1102 50-51 0.22257784262319547 1102 52-53 0.22713302192071438 1102 54-55 0.2279714157838697 1102 56-57 0.2908987741472018 1102 58-59 0.12211563247413437 1102 60-61 0.3084542396982428 1102 62-63 0.11525666626253894 1102 64-65 0.28896781612849054 1102 66-67 0.16159932360729456 1102 68-69 0.2917302567775444 1102 70-71 0.18939275464820327 1102 72-73 0.19337874859583692 1102 74-75 0.20948204454309405 1102 76-77 0.04185041555350111 1102 78-79 0.0038826415499571 1102 80-81 0.5175010466718142 1102 82-83 0.334187191704558 1102 84-85 0.4150012130635865 1102 86-87 0.1440806138783728 1102 88-89 0.25123720468073074 1102 90-91 0.3793454012191262 1102 92-93 0.45410642703096116 1102 94-95 0.42426766469111143 1102 96-97 0.7946557615200085 1102 98-99 1.0089680414861952 1102 100-101 1.4521750752297002 1103 1 0.19251022258260875 1103 2 0.1844237374459503 1103 3 0.22910635953008196 1103 4 0.15711956978881858 1103 5 -0.14574887578601903 1103 6 0.12222738464003413 1103 7 0.1918533303199581 1103 8 0.18072258691888976 1103 9 0.22610875391068674 1103 10-11 0.13221751462113218 1103 12-13 0.14734100063803623 1103 14-15 0.16119038044890033 1103 16-17 0.4209290037982214 1103 18-19 0.20697682735056588 1103 20-21 0.28461092704640123 1103 22-23 0.2025551952452318 1103 24-25 0.2789906013241179 1103 26-27 0.3473864907301518 1103 28-29 0.4390789968765958 1103 30-31 0.29186599591037066 1103 32-33 0.3681849398550696 1103 34-35 0.438363067294155 1103 36-37 0.4405422885899739 1103 38-39 0.4737086750827686 1103 40-41 0.5631291423392355 1103 42-43 0.3861956437150198 1103 44-45 0.39114135134359884 1103 46-47 0.3598049126270553 1103 48-49 0.45253286762851275 1103 50-51 0.43269282523991137 1103 52-53 0.45904945646985595 1103 54-55 0.40823836271967906 1103 56-57 0.3272418587730854 1103 58-59 0.5333524771839748 1103 60-61 0.3705966962321696 1103 62-63 0.3483325101727317 1103 64-65 0.21548388525945228 1103 66-67 0.5119681194711241 1103 68-69 0.6197263795115475 1103 70-71 0.6059394742895101 1103 72-73 0.4262802097972518 1103 74-75 0.5965428475519055 1103 76-77 0.28563728532104093 1103 78-79 0.32365858333441366 1103 80-81 0.6076668045060707 1103 82-83 0.8883845520844957 1103 84-85 0.74385935194449 1103 86-87 0.5831681052966999 1103 88-89 0.5347889877046477 1103 90-91 0.42457666254026094 1103 92-93 0.5348943226715512 1103 94-95 0.7282090281125342 1103 96-97 0.6935717608978855 1103 98-99 0.8521106378404966 1103 100-101 1.1440882734871316 1104 1 0.1549548197862869 1104 2 0.22192640521777562 1104 3 0.23428866140769955 1104 4 0.2120657428391155 1104 5 0.1794385506951528 1104 6 0.24026853284902927 1104 7 0.18169997686872819 1104 8 0.07044062204909807 1104 9 0.10549475691844634 1104 10-11 0.23202506280232882 1104 12-13 0.06510228096998105 1104 14-15 0.1529285082438605 1104 16-17 0.3369621263569087 1104 18-19 0.2576052427189168 1104 20-21 0.11999234396826353 1104 22-23 0.19713868781766308 1104 24-25 0.23119514249217588 1104 26-27 0.2588871930365926 1104 28-29 0.2360201483117308 1104 30-31 0.11613180028700754 1104 32-33 0.27216686849279625 1104 34-35 0.03256689871082585 1104 36-37 0.3042112379593931 1104 38-39 0.2576041120762014 1104 40-41 0.2891630900459461 1104 42-43 0.2920029797152921 1104 44-45 0.22838477993913386 1104 46-47 0.17222736112044856 1104 48-49 0.14298913106043898 1104 50-51 0.17138721150348601 1104 52-53 0.0712533027764195 1104 54-55 0.046260346400359253 1104 56-57 0.2959060868067809 1104 58-59 0.020508396659458583 1104 60-61 0.2599476025300973 1104 62-63 0.2695135017400929 1104 64-65 0.42157360180559067 1104 66-67 0.17175586409959465 1104 68-69 0.5596248925079372 1104 70-71 0.33688599973384825 1104 72-73 0.34906015832786963 1104 74-75 0.3668447462609521 1104 76-77 0.41437371690944147 1104 78-79 0.47562169115210295 1104 80-81 0.6256018312334213 1104 82-83 0.5291320059134286 1104 84-85 0.39477349808379003 1104 86-87 0.38784750116425926 1104 88-89 0.5300881255800611 1104 90-91 0.5606538095382092 1104 92-93 0.49170852539242915 1104 94-95 0.6339381921941509 1104 96-97 0.7088852364958385 1104 98-99 1.0326310022457186 1104 100-101 1.5507181207665006 1105 1 0.12476098402857261 1105 2 0.22571766959462636 1105 3 0.16795258448235373 1105 4 0.0995948403021103 1105 5 0.09810991388059875 1105 6 0.02855256828654973 1105 7 0.21203561671825355 1105 8 0.23793887865679864 1105 9 -0.002555663951191889 1105 10-11 0.24238596956276837 1105 12-13 0.01870471877262503 1105 14-15 0.19075060610954608 1105 16-17 0.22333898884008363 1105 18-19 0.22755812201723558 1105 20-21 0.1932129788577157 1105 22-23 0.0038744435659410215 1105 24-25 0.00923766898682743 1105 26-27 0.19984758119920798 1105 28-29 0.1828641785436318 1105 30-31 0.23625398507677176 1105 32-33 0.09274847398521757 1105 34-35 -0.04733970702714174 1105 36-37 0.024465834621899774 1105 38-39 0.1967802855510996 1105 40-41 0.08265673771372661 1105 42-43 0.02400460278897043 1105 44-45 0.18196235598541222 1105 46-47 0.038991060255135324 1105 48-49 -0.010307230897268482 1105 50-51 0.20002089531597278 1105 52-53 0.17313217629944688 1105 54-55 0.2891336165132117 1105 56-57 0.06723194892655471 1105 58-59 0.12932983891142413 1105 60-61 0.0630047391432953 1105 62-63 0.25905192021317447 1105 64-65 0.07403203049347695 1105 66-67 0.40382225051390463 1105 68-69 0.42314244429405434 1105 70-71 0.2924871999735039 1105 72-73 0.2684408489076553 1105 74-75 0.02102477484276477 1105 76-77 0.02633326529717195 1105 78-79 0.1442187760037399 1105 80-81 0.24155368659570442 1105 82-83 0.35547570711072396 1105 84-85 0.13530669374642557 1105 86-87 0.2031314040057417 1105 88-89 0.08801908724507612 1105 90-91 0.339527209791612 1105 92-93 0.14978847343443036 1105 94-95 0.13396059846839847 1105 96-97 0.1466139032672693 1105 98-99 0.5795853450113775 1105 100-101 0.820770815412569 1106 1 0.04283924249208937 1106 2 0.24037573372736887 1106 3 0.1889211061658287 1106 4 0.12495954321323666 1106 5 0.0350517638298129 1106 6 0.08841107587525698 1106 7 0.17203018184481067 1106 8 -0.05862817548704413 1106 9 0.1022782783809646 1106 10-11 0.10928434052444658 1106 12-13 0.006808350295628429 1106 14-15 0.20347984727618496 1106 16-17 0.20317950811191565 1106 18-19 0.11797206995759524 1106 20-21 0.0679058744629657 1106 22-23 0.10488803508258826 1106 24-25 0.02864722384098428 1106 26-27 0.09534929491756117 1106 28-29 0.10162659348446113 1106 30-31 0.24518949268961876 1106 32-33 0.021592486308385617 1106 34-35 0.1232086952252942 1106 36-37 0.09771205110194359 1106 38-39 0.42435740906694974 1106 40-41 0.3573108216373342 1106 42-43 0.21317827918372956 1106 44-45 0.21506463498012351 1106 46-47 0.14951150200263896 1106 48-49 0.15032314633122468 1106 50-51 0.015639718795860347 1106 52-53 -0.0676399904632703 1106 54-55 -0.03982772841368387 1106 56-57 0.07215985638266176 1106 58-59 0.12884113360598803 1106 60-61 0.07270410026711716 1106 62-63 0.21994327040782125 1106 64-65 0.25471650266389645 1106 66-67 0.1324134607512768 1106 68-69 0.2899074194440594 1106 70-71 0.45413831823895023 1106 72-73 0.30935575847395924 1106 74-75 0.32854637970270417 1106 76-77 0.006330083605888603 1106 78-79 -0.04165111015484868 1106 80-81 0.019418400339436204 1106 82-83 0.3377925602323373 1106 84-85 0.060056290309209004 1106 86-87 0.2533544628490816 1106 88-89 0.4381912947383917 1106 90-91 0.4760607633260463 1106 92-93 0.36058759172215815 1106 94-95 0.5245410590460686 1106 96-97 0.6094528013621563 1106 98-99 1.0224688796351735 1106 100-101 0.9609732180227901 1107 1 0.0625013113275763 1107 2 0.16307250494659797 1107 3 0.10197112924722873 1107 4 0.07479627043487369 1107 5 0.020442666656201425 1107 6 0.11370695266740682 1107 7 0.06417131472495186 1107 8 0.05193568579100827 1107 9 0.12134156885810654 1107 10-11 -0.00729056819910312 1107 12-13 0.0805347222704853 1107 14-15 0.13120541398690477 1107 16-17 0.06246841851775997 1107 18-19 0.06183713104271504 1107 20-21 0.1075847480176364 1107 22-23 0.046722145803975934 1107 24-25 0.0971667481512597 1107 26-27 0.11704094565430978 1107 28-29 0.13937015699723077 1107 30-31 0.015080855015298766 1107 32-33 0.19520491097453174 1107 34-35 0.24410672895758978 1107 36-37 0.2930000090579128 1107 38-39 0.38107902304984265 1107 40-41 0.21175811180793858 1107 42-43 -0.035218034847396495 1107 44-45 0.15395579112412605 1107 46-47 0.34983433750554127 1107 48-49 0.11692962092252657 1107 50-51 0.14143943306089568 1107 52-53 0.09958581983377002 1107 54-55 0.3187886587115685 1107 56-57 0.19365606026776305 1107 58-59 0.13151328466478418 1107 60-61 0.35610963773531523 1107 62-63 0.35158783995338894 1107 64-65 0.17993580637802253 1107 66-67 0.3552551065311498 1107 68-69 0.36846492010852216 1107 70-71 0.10544039507842129 1107 72-73 0.34525934816390347 1107 74-75 0.3536093137714005 1107 76-77 0.20067608322515085 1107 78-79 0.3628344665547729 1107 80-81 0.7631357891184081 1107 82-83 0.779236050942913 1107 84-85 0.6856257987757353 1107 86-87 0.72866162185408 1107 88-89 0.6545718209699913 1107 90-91 0.6759049410656033 1107 92-93 0.47531796508129887 1107 94-95 0.5565072780937292 1107 96-97 0.8311622725213539 1107 98-99 1.2685538605466427 1107 100-101 1.4809699519590218 1108 1 -0.04049484005423665 1108 2 0.05141378020978493 1108 3 -0.10134581103766038 1108 4 -0.0811576310116493 1108 5 -0.19817751673660666 1108 6 0.012492961858264096 1108 7 -0.13458838638126025 1108 8 -0.08033881785605956 1108 9 -0.04906904942431112 1108 10-11 -0.18389737690113606 1108 12-13 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-1.014855457149828 2316 76-77 -1.0587257166645436 2316 78-79 -1.3616726058751816 2316 80-81 -1.8907900978750334 2316 82-83 -1.3340642685111455 2316 84-85 -1.3284148640349436 2316 86-87 -1.4785499886312437 2316 88-89 -1.3339110526267994 2316 90-91 -1.361630282991623 2316 92-93 -1.5771283232006716 2316 94-95 -1.62253533893551 2316 96-97 -1.4153199248404924 2316 98-99 -1.615434812518032 2316 100-101 -1.5001065588360518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 48.0 5 97.0 6 228.0 7 575.0 8 1345.0 9 2369.0 10 3460.0 11 5326.0 12 7212.0 13 9661.0 14 12381.0 15 16193.0 16 19750.0 17 23918.0 18 20811.0 19 15420.0 20 14924.0 21 15460.0 22 16888.0 23 19343.0 24 22402.0 25 26910.0 26 31751.0 27 38387.0 28 46628.0 29 55671.0 30 66111.0 31 78781.0 32 93997.0 33 113374.0 34 141102.0 35 178790.0 36 227540.0 37 247474.0 38 111933.0 39 4072.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.90575104451467 20.45035448401567 15.455976517653186 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30.469120669398244 21.233895385227 20-21 22.310060496203754 25.760787385364246 30.767460543767754 21.161691574664243 22-23 22.01272298200289 25.918121168270993 30.6895352201083 21.37962062961782 24-25 21.868440668741197 26.245991930617507 30.75467065797414 21.130896742667154 26-27 21.523876799183096 26.412117275174957 30.697085277536694 21.366920648105257 28-29 21.22188568712175 26.59687285628965 30.88149813239289 21.29974332419571 30-31 21.313543579501353 26.253241958995133 30.83718266664143 21.596031794862085 32-33 21.190245680106795 26.43928160376396 30.697013071564825 21.673459644564417 34-35 21.98566097882338 26.351649170844148 30.35315667315303 21.309533177179443 36-37 22.090772202713936 26.03201462579097 30.317099218123673 21.560113953371417 38-39 21.664574217076755 26.35959919631003 30.503388940033272 21.47243764657994 40-41 21.763531547456886 26.32237652253111 30.49419384571606 21.419898084295948 42-43 21.751105100500254 26.414120439389855 30.047522871084233 21.787251589025665 44-45 21.82137430276333 26.626727802531274 29.818760617357466 21.73313727734793 46-47 22.156331935596846 26.95645640671292 29.12278034397569 21.764431313714542 48-49 22.281891225067568 27.146819566555557 28.605605901995663 21.965683306381216 50-51 22.210659767845108 27.252879159599537 28.565812616064136 21.970648456491222 52-53 22.460380095508995 27.363823956760648 28.379938141597528 21.795857806132833 54-55 22.12528722576004 27.254086237537773 28.469486034497855 22.15114050220433 56-57 22.1881690627457 27.580426555508954 28.199447327180298 22.03195705456505 58-59 22.386344073362626 27.11499973673982 28.29629844357033 22.202357746327227 60-61 22.16671246739102 27.29259740985811 28.7101322935928 21.830557829158074 62-63 22.128825688464186 27.269381950015497 28.721978081789807 21.879814279730507 64-65 21.997695535580903 27.276486919428056 28.54956299485711 22.176254550133933 66-67 21.912795721221524 27.60239259940052 28.12798117252279 22.356830506855164 68-69 21.980135520041223 27.339439337373435 28.490504811709847 22.18992033087549 70-71 22.08305010846847 27.342572823546323 28.345558590424602 22.228818477560605 72-73 22.1570512441284 27.35313605546813 28.286528154099177 22.203284546304296 74-75 21.981504467397052 27.8448817509404 27.939956669029243 22.23365711263331 76-77 22.104291988697945 27.573844355361615 28.072775913559155 22.24908774238129 78-79 22.10200991364533 27.53410422372643 28.05016752977954 22.313718332848705 80-81 22.036029640147316 27.88064072414252 27.854875685909096 22.228453949801068 82-83 22.130051104140513 27.85140160707672 27.815697336644828 22.20284995213794 84-85 21.974925691729535 27.85873855794625 27.63856480911767 22.52777094120654 86-87 22.14113829411182 27.74374849323416 27.669560150092366 22.445553062561657 88-89 22.097368083969034 27.813041635149087 27.54972874423624 22.539861536645635 90-91 21.974329246772516 27.845818096617812 27.73206968561951 22.44778297099016 92-93 22.078844819334414 27.65795260460513 27.762636119628496 22.500566456431958 94-95 21.840012068756415 27.441068676548454 27.807686732946223 22.911232521748907 96-97 22.132401291220663 27.254333969202026 27.71728857858944 22.895976160987875 98-99 21.723595484346546 27.441788371044634 27.511539385362816 23.323076759246003 100-101 21.599049617323658 27.491079069484016 27.656881326110412 23.252989987081918 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 751.0 1 543.5 2 431.0 3 525.0 4 454.0 5 411.5 6 774.0 7 1605.0 8 2518.0 9 3032.0 10 2706.0 11 2152.5 12 2129.5 13 2262.0 14 2401.5 15 2639.0 16 2855.5 17 3139.5 18 3359.5 19 3613.0 20 4063.5 21 4603.0 22 5320.5 23 6260.0 24 7394.5 25 8879.0 26 11060.0 27 13611.5 28 15845.0 29 18432.5 30 21896.5 31 25782.0 32 30499.5 33 34183.5 34 37359.0 35 40032.0 36 43365.5 37 47472.0 38 48927.5 39 49244.5 40 49772.5 41 51421.0 42 53111.0 43 54759.0 44 56025.0 45 57856.0 46 58409.5 47 59112.5 48 64458.0 49 63917.0 50 58369.5 51 54618.0 52 51750.0 53 51897.5 54 51909.5 55 48592.5 56 44383.5 57 40078.5 58 36580.5 59 32850.0 60 27699.0 61 23221.0 62 19655.0 63 15494.0 64 11820.0 65 9606.5 66 7395.0 67 5925.0 68 4413.5 69 2907.5 70 2186.5 71 1773.5 72 1447.5 73 1203.0 74 1017.5 75 859.0 76 603.0 77 365.0 78 234.0 79 163.0 80 116.0 81 84.5 82 56.0 83 33.0 84 18.0 85 11.5 86 7.5 87 2.5 88 2.5 89 2.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03395738407457846 2 0.005324328513435647 3 0.0034903931365855905 4 8.873880855726078E-4 5 7.690696741629268E-4 6 1.1831841140968105E-4 7 0.0 8 0.0 9 0.0014198209369161727 10-11 3.253756313766229E-4 12-13 9.465472912774484E-4 14-15 0.0022184702139315194 16-17 0.0028100622709799246 18-19 0.0 20-21 0.0 22-23 0.0015972985540306942 24-25 8.282288798677674E-4 26-27 0.004318622016453358 28-29 0.005294748910583227 30-31 0.0010648657026871295 32-33 3.253756313766229E-4 34-35 0.008341448004382513 36-37 0.011181089878214858 38-39 0.008873880855726077 40-41 0.0021001518025218387 42-43 5.915920570484052E-4 44-45 0.0012127637169492308 46-47 1.1831841140968105E-4 48-49 6.211716599008255E-4 50-51 0.001005706496982289 52-53 0.002484686639603302 54-55 0.0027213234624226643 56-57 0.0013606617312113321 58-59 6.507512627532458E-4 60-61 0.0035791319451428517 62-63 0.0041411443993388366 64-65 0.009258415692807543 66-67 0.0034608135337331706 68-69 0.0029875398880944465 70-71 5.9159205704840525E-5 72-73 8.282288798677674E-4 74-75 0.006389194216122777 76-77 0.002484686639603302 78-79 0.0034016543280283302 80-81 0.004673577250682401 82-83 0.004732736456387242 84-85 0.0057976021590743715 86-87 0.0036382911508476925 88-89 0.009435893309922063 90-91 0.013044604857917336 92-93 0.001242343319801651 94-95 0.0023072090224887805 96-97 9.169676884250281E-4 98-99 0.0033129155194710695 100-101 0.005176430499173546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1690354.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.631373354677855 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12850000494804 40.59378880046693 2 13.068022662599702 13.755759595253528 3 4.187606908880879 6.61198508051814 4 1.8002258497833852 3.7899343529076623 5 0.9266133432095197 2.4384466410943246 6 0.5825066931271959 1.8394876348545728 7 0.3891366499960627 1.4336557418351963 8 0.27751830923222737 1.168493579676824 9 0.19269025994598246 0.9127397711724239 >10 1.2377542170909626 12.548173031634436 >50 0.13240700691558605 4.799990799200274 >100 0.07182358691550196 6.739330201583737 >500 0.004291114771455748 1.5474697903962145 >1k 5.6462036466523E-4 0.483075410236987 >5k 3.3877221879913803E-4 1.3376695691687828 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8814 0.5214292390824644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8516 0.503799795782422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5177 0.3062672079339594 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2344 0.1386691781721462 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2083 0.12322862548318281 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.9159205704840525E-5 0.0 0.0 0.0 0.0 6 5.9159205704840525E-5 0.0 0.0 0.0 0.0 7 5.9159205704840525E-5 0.0 0.0 0.0 0.0 8 5.9159205704840525E-5 0.0 0.0 0.0 0.0 9 5.9159205704840525E-5 0.0 0.0 0.0 0.0 10-11 8.87388085572608E-5 0.0 0.0 0.0 0.0 12-13 1.1831841140968105E-4 0.0 0.0 0.0 0.0 14-15 1.1831841140968105E-4 0.0 0.0 0.0 0.0 16-17 1.1831841140968105E-4 0.0 0.0 5.9159205704840525E-5 0.0 18-19 1.1831841140968105E-4 0.0 0.0 1.1831841140968105E-4 0.0 20-21 1.1831841140968105E-4 1.1831841140968105E-4 0.0 2.0705721996694182E-4 0.0 22-23 1.1831841140968105E-4 1.1831841140968105E-4 0.0 2.957960285242026E-4 0.0 24-25 1.1831841140968105E-4 1.7747761711452156E-4 0.0 4.732736456387242E-4 0.0 26-27 1.1831841140968105E-4 1.7747761711452156E-4 0.0 5.915920570484052E-4 0.0 28-29 1.478980142621013E-4 1.7747761711452156E-4 0.0 0.0010648657026871293 0.0 30-31 1.7747761711452156E-4 1.7747761711452156E-4 0.0 0.003165017505208968 0.0 32-33 2.0705721996694182E-4 1.7747761711452156E-4 0.0 0.006980786273171181 0.0 34-35 3.253756313766229E-4 1.7747761711452156E-4 0.0 0.011861420743820524 0.0 36-37 4.141144399338837E-4 1.7747761711452156E-4 0.0 0.01816187615138604 0.0 38-39 5.324328513435648E-4 1.7747761711452156E-4 0.0 0.026355426141506454 0.0 40-41 5.324328513435648E-4 2.0705721996694182E-4 0.0 0.037625254828278575 0.0 42-43 6.507512627532458E-4 2.366368228193621E-4 0.0 0.051764304991735455 0.0 44-45 8.282288798677674E-4 2.366368228193621E-4 0.0 0.0669978004607319 0.0 46-47 0.001005706496982289 2.366368228193621E-4 0.0 0.08616538310910021 0.0 48-49 0.0010648657026871295 2.366368228193621E-4 0.0 0.10488927171468225 0.0 50-51 0.0010648657026871295 2.366368228193621E-4 0.0 0.13115595904763144 0.0 52-53 0.0010648657026871295 2.366368228193621E-4 0.0 0.16600073120778253 0.0 54-55 0.0010648657026871295 2.6621642567178233E-4 0.0 0.2100743394578887 0.0 56-57 0.0010648657026871295 2.957960285242026E-4 0.0 0.25234359193399725 0.0 58-59 0.0010648657026871295 2.957960285242026E-4 0.0 0.31173943446165714 0.0 60-61 0.0010648657026871295 2.957960285242026E-4 0.0 0.3794471453908471 0.0 62-63 0.0010648657026871295 2.957960285242026E-4 0.0 0.4393754207698506 0.0 64-65 0.0010648657026871295 4.141144399338837E-4 0.0 0.499303696148854 0.0 66-67 0.0010648657026871295 4.141144399338837E-4 0.0 0.5903497137286036 0.0 68-69 0.0010648657026871295 4.141144399338837E-4 0.0 0.6915415350867333 0.0 70-71 0.0010944453055395497 4.732736456387242E-4 0.0 0.7770265873302278 0.0 72-73 0.0011240249083919699 4.732736456387242E-4 0.0 0.8828328267333352 0.0 74-75 0.0011240249083919699 4.732736456387242E-4 0.0 1.0050557457195357 0.0 76-77 0.0011240249083919699 4.732736456387242E-4 0.0 1.134377769390317 0.0 78-79 0.0011240249083919699 4.732736456387242E-4 0.0 1.276892295933278 0.0 80-81 0.0011240249083919699 5.028532484911445E-4 0.0 1.4461467834548265 0.0 82-83 0.0011831841140968104 5.915920570484052E-4 0.0 1.6328532366593034 0.0 84-85 0.0012127637169492308 6.507512627532458E-4 0.0 1.8229613441918084 0.0 86-87 0.0012719229226540712 6.507512627532458E-4 0.0 2.037738840503232 0.0 88-89 0.0013015025255064916 6.507512627532458E-4 0.0 2.2844623078952693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7995 0.0 38.45537 1 GTATCAA 11645 0.0 29.38084 1 GAGTACT 9380 0.0 23.521646 12-13 TCAACGC 14560 0.0 23.419481 4 GTACATG 8875 0.0 23.3983 1 ATCAACG 14590 0.0 23.339468 3 TATCAAC 14745 0.0 23.031746 2 CAACGCA 14825 0.0 23.001534 5 TACACCG 145 2.337952E-5 22.927523 5 GTACTTT 9740 0.0 22.530016 14-15 TACATGG 9205 0.0 22.445595 2 AACGCAG 15295 0.0 22.326433 6 AGGTAAG 650 0.0 21.921663 2 GTAAGGT 700 0.0 21.710318 4 ACATGGG 9385 0.0 21.45649 3 GGTAAGG 720 0.0 20.448256 3 AGTACTT 9660 0.0 20.38132 12-13 ACGCAGA 17085 0.0 19.90389 7 CGCAGAG 17180 0.0 19.738537 8 ACTTTTT 11515 0.0 19.261906 16-17 >>END_MODULE