FastQCFastQC Report
Thu 26 May 2016
SRR936712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657955
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82900.5000135709352787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71660.4322192098096752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.24548314037473878No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.1170719350042673No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17350.10464698981576702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.033.355231
GTATCAA110900.029.9966451
GTACATG89550.027.5427931
CATTCCG2253.6379788E-1227.4653429
TACATGG94000.025.7831172
ACATGGG92650.025.1865353
TACCTGG12800.024.8747022
TCAACGC135300.024.3792484
ATCAACG135300.024.3777753
CAACGCA136100.024.2366775
TATCAAC138750.023.7694742
AACGCAG140050.023.5863276
GAGTACT77350.023.47191612-13
GTACCTG14650.022.3829671
GTACTTT81700.021.90025114-15
AGTACTT80600.021.25767312-13
ACGCAGA156150.021.093567
CGCAGAG156650.020.9984268
CATGGGG37100.020.8820114
CATGGGA51050.020.8550344
Produced by FastQC (version 0.11.5)