##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936712_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1657955 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36891109831087 31.0 31.0 34.0 30.0 34.0 2 31.416219981845103 31.0 31.0 34.0 28.0 34.0 3 31.360508578338976 31.0 31.0 34.0 28.0 34.0 4 35.05511488550654 37.0 35.0 37.0 32.0 37.0 5 35.09996230295756 37.0 35.0 37.0 32.0 37.0 6 35.175084969133664 37.0 35.0 37.0 32.0 37.0 7 35.04706882876797 37.0 35.0 37.0 32.0 37.0 8 35.031921855538904 37.0 35.0 37.0 32.0 37.0 9 36.52095322249398 39.0 37.0 39.0 32.0 39.0 10-11 36.38750418437171 39.0 37.0 39.0 32.0 39.0 12-13 36.27794873805381 39.0 35.0 39.0 32.0 39.0 14-15 37.237532080183115 39.0 36.5 41.0 32.0 41.0 16-17 37.1463121737321 39.0 36.0 41.0 31.0 41.0 18-19 37.046346855011144 39.0 36.0 41.0 31.0 41.0 20-21 37.04713547713901 39.0 36.0 41.0 31.0 41.0 22-23 37.04191488912546 39.0 36.0 41.0 31.0 41.0 24-25 37.149263399790705 39.0 36.0 41.0 31.0 41.0 26-27 37.06909807564138 39.0 36.0 41.0 31.0 41.0 28-29 36.947388198111526 39.0 36.0 41.0 30.5 41.0 30-31 36.731498140781866 39.0 36.0 41.0 30.0 41.0 32-33 36.52926044434258 39.0 35.0 40.5 30.0 41.0 34-35 36.319818993881015 39.0 35.0 40.0 30.0 41.0 36-37 36.09604663576515 38.5 35.0 40.0 29.5 41.0 38-39 35.89263128372001 38.0 35.0 40.0 29.0 41.0 40-41 35.641624169534154 38.0 35.0 40.0 28.0 41.0 42-43 35.3349424441556 38.0 34.5 40.0 27.0 41.0 44-45 35.13067453579862 38.0 34.0 40.0 26.0 41.0 46-47 34.891754601301 38.0 34.0 40.0 24.5 41.0 48-49 34.60530050574352 38.0 34.0 40.0 24.0 41.0 50-51 33.601820917938056 37.5 32.5 39.5 22.5 40.5 52-53 33.708542451393434 37.5 33.0 39.5 23.0 40.5 54-55 34.139392504621654 38.0 33.5 40.0 23.0 41.0 56-57 34.00770587862759 38.0 33.0 40.0 22.0 41.0 58-59 33.78130075906765 38.0 33.0 40.0 22.0 41.0 60-61 33.49374771932893 37.0 33.0 40.0 20.5 41.0 62-63 33.15779197867252 37.0 32.5 40.0 19.5 41.0 64-65 32.79772340021292 36.0 32.0 39.0 18.5 41.0 66-67 32.43851461589729 36.0 32.0 39.0 17.5 41.0 68-69 32.00861151237518 35.0 31.0 39.0 14.0 40.0 70-71 31.618276129328 35.0 31.0 38.0 12.0 40.0 72-73 31.206478161349374 35.0 31.0 37.0 8.0 39.5 74-75 30.80155372130124 35.0 30.0 37.0 7.0 39.0 76-77 30.335863156720176 34.5 30.0 36.0 4.5 39.0 78-79 29.875711041614522 34.0 29.0 36.0 2.0 38.5 80-81 29.436507625357745 34.0 29.0 35.0 2.0 37.0 82-83 29.038406651567747 34.0 29.0 35.0 2.0 37.0 84-85 28.666917678706596 34.0 29.0 35.0 2.0 36.0 86-87 28.306403671993507 34.0 28.0 35.0 2.0 36.0 88-89 27.946985292121923 34.0 27.0 35.0 2.0 36.0 90-91 27.58073892234711 33.5 26.5 35.0 2.0 35.0 92-93 27.15086808749333 33.0 26.0 35.0 2.0 35.0 94-95 26.64868769055855 33.0 25.0 35.0 2.0 35.0 96-97 26.028308066262355 33.0 24.0 35.0 2.0 35.0 98-99 25.26608050278807 32.5 19.5 35.0 2.0 35.0 100-101 22.639700715640654 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48448268431413766 1101 2 0.34891770035788383 1101 3 0.5028012757540274 1101 4 0.5493831471557016 1101 5 0.3861608866145616 1101 6 0.47249046411963747 1101 7 0.44516664991596144 1101 8 0.3981360087131378 1101 9 0.47025452506420606 1101 10-11 0.612683237396098 1101 12-13 0.6918234383215349 1101 14-15 0.9258629901146875 1101 16-17 0.9807278267772759 1101 18-19 1.0303873899781948 1101 20-21 0.9337513366776378 1101 22-23 0.9199385429866851 1101 24-25 0.6886580069656318 1101 26-27 0.6384063832455666 1101 28-29 0.5497274090354196 1101 30-31 0.5400971093055134 1101 32-33 0.5647297392654096 1101 34-35 0.5196087278930079 1101 36-37 0.4750721861474432 1101 38-39 0.47064372484405936 1101 40-41 0.5539944825881307 1101 42-43 0.5229910807114351 1101 44-45 0.5068678182226662 1101 46-47 0.6531044700381088 1101 48-49 0.5722803848681224 1101 50-51 0.5381599153341909 1101 52-53 0.5970174977542015 1101 54-55 0.4337424230138964 1101 56-57 0.5488539700139583 1101 58-59 0.533165261345772 1101 60-61 0.39605606191305043 1101 62-63 0.4826392752023878 1101 64-65 0.14975914390748102 1101 66-67 0.3673286131216571 1101 68-69 0.47532037914537284 1101 70-71 0.48919575592025666 1101 72-73 0.550657792376331 1101 74-75 0.23873608035180993 1101 76-77 0.34306556926831533 1101 78-79 0.3795415787792109 1101 80-81 0.43795498687178736 1101 82-83 0.3229264993409622 1101 84-85 0.4254647545125749 1101 86-87 0.33574007433861297 1101 88-89 0.27344740459449923 1101 90-91 0.26325292580131787 1101 92-93 0.389051494329415 1101 94-95 0.5632741821414413 1101 96-97 0.6259450413455383 1101 98-99 0.7977548762498436 1101 100-101 1.101715125647221 1102 1 0.18475598982000463 1102 2 0.3918912642616803 1102 3 0.44436855860038094 1102 4 0.28124725634539516 1102 5 0.2419549633388769 1102 6 0.2532315361397792 1102 7 0.38240742378464176 1102 8 0.2456828847483834 1102 9 0.36501037214564036 1102 10-11 0.3685874645212479 1102 12-13 0.4253878390370929 1102 14-15 0.5968760425230926 1102 16-17 0.3161664407042579 1102 18-19 0.47042830298422444 1102 20-21 0.6208314400368167 1102 22-23 0.4642372244946742 1102 24-25 0.5260420591752606 1102 26-27 0.39397601817566397 1102 28-29 0.1621089766495487 1102 30-31 0.25901813229629767 1102 32-33 0.29840263398481426 1102 34-35 0.1749373570588446 1102 36-37 0.21368611313346264 1102 38-39 0.18691865361898863 1102 40-41 0.3945397684822609 1102 42-43 0.24996302134616855 1102 44-45 0.29108649575530166 1102 46-47 0.4891865942016338 1102 48-49 0.6093124527141427 1102 50-51 0.5653066058529674 1102 52-53 0.2193068040551296 1102 54-55 0.5206494328860245 1102 56-57 0.255266711019722 1102 58-59 0.2586522420653097 1102 60-61 0.2929229869776506 1102 62-63 0.3327684741687946 1102 64-65 0.4361352785394246 1102 66-67 0.29532751989963657 1102 68-69 0.49188683379323095 1102 70-71 0.2730293211491741 1102 72-73 0.46741390448127973 1102 74-75 0.3157444617206515 1102 76-77 0.5461829084321685 1102 78-79 0.483046514842286 1102 80-81 0.672032870658974 1102 82-83 0.4140134270557958 1102 84-85 0.5872375962272756 1102 86-87 0.3691073638105564 1102 88-89 0.5453623651722488 1102 90-91 0.4650020810388469 1102 92-93 0.44825692068600276 1102 94-95 0.5779705645703785 1102 96-97 0.550352051217665 1102 98-99 0.556403753212674 1102 100-101 1.3954117068438023 1103 1 0.28855245175600075 1103 2 0.5120406239459214 1103 3 0.49164402215491165 1103 4 0.4067198027481709 1103 5 0.4018862465259687 1103 6 0.40125569114067616 1103 7 0.37830618479968336 1103 8 0.31627000649829995 1103 9 0.3878071275005297 1103 10-11 0.27429730162644006 1103 12-13 0.37583506622851104 1103 14-15 0.6711010853527881 1103 16-17 0.6206779928902364 1103 18-19 0.6730368916393346 1103 20-21 0.7769960764340098 1103 22-23 0.5928067600409506 1103 24-25 0.4776530235769272 1103 26-27 0.5909396058369296 1103 28-29 0.4103309527785015 1103 30-31 0.5580096231482585 1103 32-33 0.5140929729309747 1103 34-35 0.4830361919018671 1103 36-37 0.5881397387498737 1103 38-39 0.3428059618318784 1103 40-41 0.44746070628691825 1103 42-43 0.6290403608886166 1103 44-45 0.8509292800166932 1103 46-47 0.7419887446782027 1103 48-49 0.7927399640486286 1103 50-51 0.8029716539831355 1103 52-53 0.9251874866800023 1103 54-55 0.9131582591157823 1103 56-57 0.9702963841889343 1103 58-59 0.8277112192638256 1103 60-61 0.6117122080924489 1103 62-63 0.6062826860550956 1103 64-65 0.7967967961776878 1103 66-67 0.6575971624018351 1103 68-69 0.7706276881154821 1103 70-71 0.8443452575813879 1103 72-73 0.8426289994638161 1103 74-75 0.681607065955351 1103 76-77 0.7708618040412958 1103 78-79 0.853738699487959 1103 80-81 0.776950003483087 1103 82-83 0.7918356909245752 1103 84-85 0.7855561166388192 1103 86-87 0.5230462758890049 1103 88-89 0.4734889328337033 1103 90-91 0.8117579091900282 1103 92-93 1.0253887036317444 1103 94-95 1.0040133847992436 1103 96-97 1.038984908455177 1103 98-99 1.392065507479078 1103 100-101 1.7242926450270666 1104 1 0.2790589466229605 1104 2 0.3031236504940651 1104 3 0.4371036013354228 1104 4 0.42735297745111467 1104 5 0.3824839538678617 1104 6 0.3364099068181474 1104 7 0.3136194049966221 1104 8 0.30968534875085396 1104 9 0.42029642743169404 1104 10-11 0.5951038847876688 1104 12-13 0.5080108466601061 1104 14-15 0.6004151585622068 1104 16-17 0.5916577974456203 1104 18-19 0.5554633380192655 1104 20-21 0.5869725811779531 1104 22-23 0.36982069257813777 1104 24-25 0.5763125219457308 1104 26-27 0.14771918441883258 1104 28-29 0.09970907674964735 1104 30-31 0.2523889069170693 1104 32-33 0.1377891358713228 1104 34-35 0.3427296686011587 1104 36-37 0.5228356472829958 1104 38-39 0.44220904015937634 1104 40-41 0.5566696868621719 1104 42-43 0.39756158144472664 1104 44-45 0.5252721236909323 1104 46-47 0.5530586393236803 1104 48-49 0.5768333389850326 1104 50-51 0.4979729260192869 1104 52-53 0.5445334730317981 1104 54-55 0.6745841368207266 1104 56-57 0.6349502407032546 1104 58-59 0.7015027849158528 1104 60-61 0.5432420560467222 1104 62-63 0.4831813870817072 1104 64-65 0.4127344738886265 1104 66-67 0.5412142720363846 1104 68-69 0.3771326565390467 1104 70-71 0.3872839604038134 1104 72-73 0.628332996650375 1104 74-75 0.6313822148584087 1104 76-77 0.5021610019102596 1104 78-79 0.538517851563622 1104 80-81 0.513906956283062 1104 82-83 0.4985864616288289 1104 84-85 0.6218375808272611 1104 86-87 0.4555572752604675 1104 88-89 0.6415426801025639 1104 90-91 0.8018855991723655 1104 92-93 0.7310834972625813 1104 94-95 0.76928062463044 1104 96-97 0.6246696380352503 1104 98-99 0.6155398123487323 1104 100-101 1.0614561166382117 1105 1 0.29689407075319707 1105 2 0.3317670217942954 1105 3 0.4339109305969444 1105 4 0.4960816867267468 1105 5 0.46412403370339206 1105 6 0.4120676370080787 1105 7 0.4292145948561128 1105 8 0.33480652767547525 1105 9 0.48170875591787166 1105 10-11 0.47851278299737743 1105 12-13 0.405146947224857 1105 14-15 0.8887544070277755 1105 16-17 0.7843333818306704 1105 18-19 0.6164192133838 1105 20-21 0.5624873102323775 1105 22-23 0.5544000178779029 1105 24-25 0.37425854542618 1105 26-27 0.3010858700939565 1105 28-29 0.41508160502473146 1105 30-31 0.21109287393239384 1105 32-33 0.3447134046709337 1105 34-35 0.21529749253280528 1105 36-37 0.37640736169591094 1105 38-39 0.12566177037431459 1105 40-41 0.23377896924284158 1105 42-43 0.2975480129067236 1105 44-45 0.20412499446842247 1105 46-47 0.46260323041671825 1105 48-49 0.7037199597812887 1105 50-51 0.5960331253079687 1105 52-53 0.6414061126064112 1105 54-55 0.6155266062196105 1105 56-57 0.48282879666064815 1105 58-59 0.5896355039758348 1105 60-61 0.4613232584317828 1105 62-63 0.5835057189093646 1105 64-65 0.7335824074690649 1105 66-67 0.6105218326508606 1105 68-69 0.4811267464090392 1105 70-71 0.7957851184191327 1105 72-73 0.6740569375540737 1105 74-75 0.5275538237243502 1105 76-77 0.6205089792601832 1105 78-79 0.6004227429903075 1105 80-81 0.7107215700361813 1105 82-83 0.5538372982511284 1105 84-85 0.7438881433275739 1105 86-87 0.7536718112289726 1105 88-89 0.5322492007542543 1105 90-91 0.5805869650301716 1105 92-93 0.5984327489295715 1105 94-95 0.6517197162762187 1105 96-97 0.6594435499260456 1105 98-99 0.9994956727065905 1105 100-101 1.431427785280757 1106 1 0.15432222184357158 1106 2 0.02372522476458272 1106 3 0.12168835673929124 1106 4 0.2762483745547044 1106 5 0.23582620396779674 1106 6 0.2020146327044401 1106 7 0.35452122832165145 1106 8 0.5200749192086818 1106 9 0.4358853952390902 1106 10-11 0.5031391598238031 1106 12-13 0.479242744086541 1106 14-15 0.42783536887490925 1106 16-17 0.2680819818689528 1106 18-19 0.357964644786783 1106 20-21 0.22422544837962732 1106 22-23 0.0633202928610146 1106 24-25 -0.23124866552160483 1106 26-27 -0.18975160548369274 1106 28-29 0.11279789081969227 1106 30-31 0.07453304337435185 1106 32-33 0.11676363084411179 1106 34-35 0.21057031214572675 1106 36-37 -0.02253608964164755 1106 38-39 0.2064941230238091 1106 40-41 0.009479044854977303 1106 42-43 -0.07515195974151112 1106 44-45 0.019992206401624912 1106 46-47 0.25372720464667964 1106 48-49 0.15598213368416936 1106 50-51 0.0676563039364595 1106 52-53 0.19704957701974735 1106 54-55 0.3566552864686727 1106 56-57 0.5310929704637246 1106 58-59 0.3352004757807734 1106 60-61 0.43676068132729284 1106 62-63 0.31640518254953065 1106 64-65 0.07065828934229756 1106 66-67 0.09393753711033526 1106 68-69 0.33982252217259656 1106 70-71 0.19879043274646335 1106 72-73 0.07986249116195054 1106 74-75 0.20702291528579764 1106 76-77 0.17049764059672512 1106 78-79 0.3608310991115111 1106 80-81 0.39949657747421696 1106 82-83 0.2279011004997855 1106 84-85 0.5962941192769726 1106 86-87 0.5560515408571227 1106 88-89 0.6521246144259649 1106 90-91 0.7027970695164321 1106 92-93 0.8057085702878233 1106 94-95 0.89194477769135 1106 96-97 0.7556980640722131 1106 98-99 0.9347984908224269 1106 100-101 1.5009614282638317 1107 1 0.2849170903197056 1107 2 0.33507970361264583 1107 3 0.27062504603068405 1107 4 0.3622486519066115 1107 5 0.3732258244065747 1107 6 0.4203368077597176 1107 7 0.40114202920096176 1107 8 0.368986184575391 1107 9 0.4837407973094727 1107 10-11 0.5959599185867077 1107 12-13 0.4968716035795566 1107 14-15 0.39494798145281607 1107 16-17 0.4959910951903197 1107 18-19 0.6550428837049012 1107 20-21 0.5083471159836392 1107 22-23 0.48995140455422614 1107 24-25 0.5515517284208258 1107 26-27 0.6277450624413206 1107 28-29 0.5147780678626575 1107 30-31 0.3597477471342714 1107 32-33 0.4879317446200204 1107 34-35 0.5072222509125979 1107 36-37 0.5529004186005864 1107 38-39 0.6556042214580557 1107 40-41 0.598184466314315 1107 42-43 0.6067376028385851 1107 44-45 0.5819209706191231 1107 46-47 0.7418315685734669 1107 48-49 0.7009734921178463 1107 50-51 0.5847240394351871 1107 52-53 0.775518337693299 1107 54-55 0.8548990454087786 1107 56-57 0.9305570777886416 1107 58-59 1.1536335273964866 1107 60-61 0.9652768871532231 1107 62-63 0.912803588289691 1107 64-65 0.9226579837415869 1107 66-67 0.9874379364457226 1107 68-69 0.8902890126694416 1107 70-71 0.9828292799897653 1107 72-73 1.0804008235065758 1107 74-75 0.9722056850554672 1107 76-77 0.9751276197696548 1107 78-79 1.0539588710255252 1107 80-81 1.0807951622090783 1107 82-83 1.0419944294160324 1107 84-85 1.064788997254972 1107 86-87 1.1241889430456347 1107 88-89 1.176607019272435 1107 90-91 1.0850624696093973 1107 92-93 1.0139642194068479 1107 94-95 1.011648768733913 1107 96-97 1.2662043725440277 1107 98-99 1.3383272160052115 1107 100-101 1.776799775760086 1108 1 0.05301724763349824 1108 2 0.1569250695939921 1108 3 0.16586364725964842 1108 4 0.190785037524968 1108 5 0.1462922512582523 1108 6 0.3155183924564824 1108 7 0.2890876976290926 1108 8 0.34997765023717875 1108 9 -0.17551350419913803 1108 10-11 0.09733900595936262 1108 12-13 0.08118577244697178 1108 14-15 0.5787466042444009 1108 16-17 0.6356119482094726 1108 18-19 0.5584008334940762 1108 20-21 0.34730165307419014 1108 22-23 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-6.329124323107543 2316 90-91 -5.271809000599816 2316 92-93 -4.8065323513446465 2316 94-95 -4.105171197881731 2316 96-97 -5.4931296267467715 2316 98-99 -7.200065448084114 2316 100-101 -7.819501349448565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3766.0 3 1477.0 4 1445.0 5 1590.0 6 1780.0 7 2207.0 8 2581.0 9 3497.0 10 4386.0 11 5637.0 12 7427.0 13 8948.0 14 10448.0 15 12925.0 16 16146.0 17 20537.0 18 15726.0 19 11500.0 20 10946.0 21 12043.0 22 13182.0 23 14829.0 24 16964.0 25 19685.0 26 22607.0 27 27091.0 28 32655.0 29 39917.0 30 48298.0 31 59204.0 32 73846.0 33 94529.0 34 124013.0 35 166759.0 36 224900.0 37 281801.0 38 211948.0 39 30707.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4825912807921 18.910750889086867 14.194908604525953 22.41174922559508 2 15.771605981041374 20.173224318010696 39.94228671544684 24.11288298550109 3 19.379089264966876 25.091482505215073 29.799227119118488 25.730201110699564 4 12.565703071884423 16.71519931565647 37.1825847119441 33.53651290051501 5 13.399888752544392 37.799942807707204 33.819226504404654 14.98094193534375 6 31.299140390082393 36.14669356668131 17.48812666817899 15.066039375057317 7 28.393017639400032 33.50496079831519 20.453869132951326 17.648152429333447 8 27.00158956415527 33.807989822927134 19.755856463684434 19.434564149233157 9 27.20773518343599 16.407388741855755 20.562695967335507 35.822180107372745 10-11 25.24090295987616 26.66516796972833 26.689157179110378 21.404771891285126 12-13 26.37258803216852 24.339775706111226 27.433478274904672 21.854157986815576 14-15 23.094691357242485 25.319365459804498 26.3607709604844 25.22517222246862 16-17 21.843061596914122 28.8823339142693 26.98208190714358 22.292522581672998 18-19 21.75840661184155 26.749029054737417 29.9936095025912 21.498954830829835 20-21 22.57724741342308 25.855091366092065 30.198272307482092 21.36938891300276 22-23 22.233268013696915 26.003116883743616 30.102177927794106 21.661437174765368 24-25 22.030327682675104 26.33080647890663 30.237974365865718 21.400891472552544 26-27 21.68098988576617 26.461104089432393 30.15429259184406 21.70361343295738 28-29 21.407724664330903 26.663522023011193 30.347912395937982 21.58084091671992 30-31 21.489604698370535 26.34349988675682 30.33846591832679 21.828429496545855 32-33 21.275425909047314 26.595405858462414 30.158999207989833 21.97016902450044 34-35 22.161310647122818 26.580754402056005 29.766859404750885 21.49107554607029 36-37 22.157186096477314 26.171585191225915 29.817170341435638 21.854058370861136 38-39 21.728436541836043 26.523184740193738 29.96339108682447 21.784987631145746 40-41 21.950615601016228 26.42177536293076 30.01555589869545 21.61205313735756 42-43 21.843565714451156 26.53485716767305 29.56657215081762 22.05500496705818 44-45 21.833839231457063 26.809976823683574 29.41187646736844 21.944307477490927 46-47 22.164319180864975 27.108907176599452 28.756339176715258 21.97043446582032 48-49 22.328398287511355 27.217513562027047 28.34934116957329 22.104746980888308 50-51 22.1387881692195 27.42556302906728 28.308682606829784 22.126966194883437 52-53 22.464780364702737 27.393026219681737 28.21057050471274 21.93162291090278 54-55 22.006839166790567 27.373495167678406 28.285434335946704 22.334231329584327 56-57 22.121116801390677 27.75998245969061 28.017990277433046 22.10091046148566 58-59 22.314163243382463 27.251895711799328 28.105090426877837 22.328850617940372 60-61 22.118792895817734 27.385254017432626 28.551540728350894 21.94441235839875 62-63 22.01051929995989 27.421203265567083 28.579623078523795 21.988654355949226 64-65 21.86635318612897 27.48086561401784 28.38653525494973 22.266245944903464 66-67 21.790962494869408 27.753338241010795 28.04059117709769 22.41510808702211 68-69 21.90483572247819 27.471370825581214 28.424907585317516 22.198885866623076 70-71 21.917480193376583 27.61828934709769 28.153724127739526 22.3105063317862 72-73 22.089250594239797 27.553300594843083 28.084737991529817 22.2727108193873 74-75 21.887077348417918 28.02471172718166 27.887169646393183 22.20104127800723 76-77 22.08463798543616 27.64176214005176 27.980971686786965 22.292628187725107 78-79 22.143521180205948 27.71102835469643 27.881816520059914 22.263633945037704 80-81 21.999285161675616 28.074234602699192 27.725321111709324 22.20115912391587 82-83 22.029678559181743 28.042537880832768 27.68011359198149 22.247669968004 84-85 21.945766656020616 27.977072082220094 27.619449161781695 22.45771209997759 86-87 22.100088333281562 27.93602607596059 27.616439942566778 22.347445648191066 88-89 22.105841561256067 27.89773219057718 27.438482461322806 22.55794378684395 90-91 22.028272779969708 27.97365743200979 27.646305544111677 22.35176424390882 92-93 22.192721591526016 27.777962093884597 27.74418197830834 22.28513433628105 94-95 21.917847470657982 27.573913418531994 27.769994392946757 22.738244717863275 96-97 22.101042540703606 27.437797725508318 27.70321339963095 22.75794633415713 98-99 21.87146134712688 27.647900230877774 27.512070256342504 22.968568165652844 100-101 22.00361418636014 27.40082328161003 27.654890146169137 22.940672385860694 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 634.0 1 442.5 2 371.0 3 522.5 4 493.0 5 474.5 6 794.5 7 1518.0 8 2373.5 9 2833.5 10 2580.5 11 2196.0 12 2166.5 13 2267.5 14 2425.5 15 2630.0 16 2755.5 17 2907.5 18 3130.5 19 3338.0 20 3648.5 21 4189.5 22 4980.5 23 5901.5 24 6963.5 25 8352.5 26 10406.5 27 12913.0 28 15015.5 29 17242.0 30 20548.0 31 24405.0 32 28970.5 33 32834.5 34 35930.5 35 38596.5 36 42256.5 37 46249.0 38 47761.5 39 48287.5 40 48593.5 41 50215.5 42 52039.0 43 53660.5 44 55278.0 45 57387.5 46 57501.0 47 57875.5 48 63296.5 49 62778.5 50 57452.5 51 53923.0 52 51493.0 53 51709.0 54 51196.0 55 48221.5 56 44169.5 57 39707.0 58 36371.5 59 32844.5 60 28024.5 61 23464.5 62 19868.0 63 15825.0 64 11940.0 65 9623.5 66 7486.5 67 6010.5 68 4488.5 69 3001.5 70 2289.0 71 1788.5 72 1468.5 73 1275.0 74 1028.5 75 891.0 76 673.5 77 388.5 78 244.5 79 158.5 80 101.0 81 76.0 82 51.5 83 25.0 84 11.0 85 7.5 86 9.0 87 9.0 88 5.0 89 2.5 90 2.0 91 2.0 92 3.0 93 2.0 94 1.5 95 2.0 96 1.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07231800621850412 2 0.027202185825308887 3 0.015742284923294058 4 0.018577102514845095 5 0.023462639215177734 6 0.02768470796855162 7 0.07635912916816198 8 0.05410279531109107 9 0.06694994737492875 10-11 0.05790265718912757 12-13 0.03742562373526423 14-15 0.043638096329514375 16-17 0.05181081513068811 18-19 0.04867442119961036 20-21 0.018727890684608447 22-23 0.012636048626168985 24-25 0.02174365407987551 26-27 0.023522954483083076 28-29 0.006604521835634864 30-31 0.0011158324562488126 32-33 0.0089266596499905 34-35 0.010766275321103407 36-37 0.004403014557089909 38-39 0.009771073400665279 40-41 0.003799861878036497 42-43 0.001930088572970919 44-45 7.539408488167652E-4 46-47 6.634679469587534E-4 48-49 0.0010253595543908006 50-51 0.0015380393315862011 52-53 1.2063053581068243E-4 54-55 2.714187055740355E-4 56-57 0.003588758440367803 58-59 0.0041315958515158735 60-61 0.005217270673812015 62-63 0.0030157633952670607 64-65 6.333103130060827E-4 66-67 5.126797771954003E-4 68-69 0.0044934874589479205 70-71 0.0030157633952670607 72-73 0.022618225464502956 74-75 0.01691843264744821 76-77 0.025633988859770016 78-79 0.02086908269524806 80-81 0.01432487612751854 82-83 0.005518847013338722 84-85 0.0038300195119891677 86-87 0.0017793004032075658 88-89 0.002020561474828931 90-91 0.006393418397966169 92-93 0.010555171883434713 94-95 0.01335983184103308 96-97 0.010736117687150737 98-99 0.02219601858916557 100-101 0.01933104336366186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1657955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.368180261554464 #Duplication Level Percentage of deduplicated Percentage of total 1 78.17848235086866 42.504218210267815 2 12.599825814847845 13.700592023316702 3 3.9810915882277484 6.493341153195733 4 1.6644263730083124 3.6196733231920493 5 0.902277908295204 2.4527603982105983 6 0.5354988467894211 1.7468458699261071 7 0.3403508859935255 1.2952980825313036 8 0.2533994141609256 1.1021492021818802 9 0.18779984588448073 0.9189302286725636 >10 1.164559616436648 12.160167074812346 >50 0.12299800526025308 4.642734591184173 >100 0.06493101977310049 6.4376309107320315 >500 0.0030173056987850113 1.0495261434907783 >1k 0.0011175206291796338 0.9370638943532636 >5k 2.2350412583592674E-4 0.9390688939327007 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8290 0.5000135709352787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7166 0.4322192098096752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4070 0.24548314037473878 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.1170719350042673 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1735 0.10464698981576702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 6.0315267905341216E-5 0.0 0.0 0.0 18-19 6.0315267905341216E-5 6.0315267905341216E-5 0.0 0.0 0.0 20-21 1.2063053581068243E-4 6.0315267905341216E-5 0.0 3.0157633952670608E-5 0.0 22-23 1.2063053581068243E-4 6.0315267905341216E-5 0.0 1.2063053581068243E-4 0.0 24-25 3.618916074320473E-4 9.047290185801182E-5 0.0 1.2063053581068243E-4 0.0 26-27 3.618916074320473E-4 1.2063053581068243E-4 0.0 4.5236450929005915E-4 0.0 28-29 4.5236450929005915E-4 1.2063053581068243E-4 0.0 0.0013570935278701774 0.0 30-31 4.8252214324272973E-4 1.2063053581068243E-4 0.0 0.003528443172462461 0.0 32-33 4.8252214324272973E-4 1.2063053581068243E-4 0.0 0.00796161536350504 0.0 34-35 4.8252214324272973E-4 1.2063053581068243E-4 0.0 0.013209043671269727 0.0 36-37 4.8252214324272973E-4 1.2063053581068243E-4 0.0 0.020326245284099993 0.0 38-39 5.42837411148071E-4 1.2063053581068243E-4 0.0 0.029162432032232483 0.0 40-41 5.42837411148071E-4 1.8094580371602365E-4 0.0 0.04216037226583351 0.0 42-43 5.729950451007416E-4 2.4126107162136486E-4 0.0 0.05708840107240547 0.0 44-45 6.031526790534122E-4 3.015763395267061E-4 0.0 0.07457982876495442 0.0 46-47 6.93625580911424E-4 3.015763395267061E-4 0.0 0.0961425370411139 0.0 48-49 7.237832148640946E-4 3.618916074320473E-4 0.0 0.11927344228281225 0.0 50-51 7.237832148640946E-4 3.618916074320473E-4 0.0 0.14789303690389666 0.0 52-53 7.237832148640946E-4 3.618916074320473E-4 0.0 0.18260447358342052 0.0 54-55 7.237832148640946E-4 3.618916074320473E-4 0.0 0.2272679294673257 0.0 56-57 7.840984827694359E-4 3.920492413847179E-4 0.0 0.27196154298518355 0.0 58-59 7.840984827694359E-4 4.8252214324272973E-4 0.0 0.3321260227207614 0.0 60-61 7.840984827694359E-4 4.8252214324272973E-4 0.0 0.4077312110401066 0.0 62-63 7.840984827694359E-4 4.8252214324272973E-4 0.0 0.47133366104628893 0.0 64-65 7.840984827694359E-4 5.42837411148071E-4 0.0 0.5385248694928391 0.0 66-67 8.44413750674777E-4 5.42837411148071E-4 0.0 0.6311389633614906 0.0 68-69 8.44413750674777E-4 5.42837411148071E-4 0.0 0.7322273523708425 0.0 70-71 9.047290185801183E-4 5.42837411148071E-4 0.0 0.8294254066002997 0.0 72-73 9.9520192043813E-4 5.42837411148071E-4 0.0 0.9458941889255137 0.0 74-75 0.0010253595543908006 5.42837411148071E-4 0.0 1.0730086160360202 0.0 76-77 0.0010253595543908006 5.42837411148071E-4 0.0 1.2130003528443174 0.0 78-79 0.0010253595543908006 5.42837411148071E-4 0.0 1.3658693993504047 0.0 80-81 0.001085674822296142 6.031526790534123E-4 0.0 1.538310750291775 0.0 82-83 0.001085674822296142 6.634679469587534E-4 0.0 1.7343655286180866 0.0 84-85 0.001085674822296142 6.634679469587534E-4 0.0 1.9301488882388242 0.0 86-87 0.0011158324562488126 6.634679469587534E-4 0.0 2.146982276358526 0.0 88-89 0.0011459900902014832 6.634679469587534E-4 0.0 2.3981048942824144 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7480 0.0 33.35523 1 GTATCAA 11090 0.0 29.996645 1 GTACATG 8955 0.0 27.542793 1 CATTCCG 225 3.6379788E-12 27.465342 9 TACATGG 9400 0.0 25.783117 2 ACATGGG 9265 0.0 25.186535 3 TACCTGG 1280 0.0 24.874702 2 TCAACGC 13530 0.0 24.379248 4 ATCAACG 13530 0.0 24.377775 3 CAACGCA 13610 0.0 24.236677 5 TATCAAC 13875 0.0 23.769474 2 AACGCAG 14005 0.0 23.586327 6 GAGTACT 7735 0.0 23.471916 12-13 GTACCTG 1465 0.0 22.382967 1 GTACTTT 8170 0.0 21.900251 14-15 AGTACTT 8060 0.0 21.257673 12-13 ACGCAGA 15615 0.0 21.09356 7 CGCAGAG 15665 0.0 20.998426 8 CATGGGG 3710 0.0 20.882011 4 CATGGGA 5105 0.0 20.855034 4 >>END_MODULE