FastQCFastQC Report
Thu 26 May 2016
SRR936712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657955
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87700.5289648995298425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84640.510508427550808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.3155694816807453No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.14300750020356404No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21270.12829057483466078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.036.6739541
GTATCAA119700.028.8184261
GTACATG89400.023.7573411
GAGTACT91850.023.24104312-13
ATCAACG148900.023.1246193
TCAACGC149150.023.0851614
CAACGCA150500.022.8780865
TATCAAC152150.022.5736832
TACATGG92250.022.5035552
AACGCAG154700.022.3163436
GTACTTT97350.021.87854614-15
ACATGGG92400.021.587813
CCTATAC5400.021.108083
AGTACTT95100.020.44930312-13
ACGCAGA173700.019.8485497
CGCAGAG175600.019.5256128
ACTTTTT110950.019.36793116-17
AGAGTAC150050.019.24241610-11
GTATAGG5750.019.005871
CATGGGG37750.018.99674
Produced by FastQC (version 0.11.5)