FastQCFastQC Report
Thu 26 May 2016
SRR936707_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936707_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005282
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104031.034834006776208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78420.7800796194500648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49740.49478653750887813No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22990.2286920485992985No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21510.21396981145589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.18780799815375188No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17360.17268786270917016No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15610.15527981203284252No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14260.14185074436824693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.12872010042953122No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.12275162591193318No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11370.11310259210848299No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10850.10792991419323135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA250.005021393856.9991382
GTATCAA128800.027.7693771
GTACATG92550.027.7143271
TACATGG93850.026.8243142
ACATGGG93550.025.6917533
GTCTTAG2600.025.5765341
GAGTACT71600.024.382912-13
GGTATCA92250.024.2001761
CATGGGA63000.023.8250334
CATGGGG24700.023.2688774
ATCAACG153600.023.1930073
GTCCTAT1852.5413465E-723.1077581
GTATAGT1852.5413465E-723.1077581
TCAACGC154400.023.0420724
TATCAAC155250.023.0382982
CAACGCA155800.022.9264815
AGTACTT72950.022.75951212-13
GTACTTT77200.022.67459914-15
AGGGTCG1050.002196224922.6187045
AACGCAG158250.022.5437646
Produced by FastQC (version 0.11.5)