##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936707_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1005282 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.657442389299717 31.0 31.0 34.0 30.0 34.0 2 31.69774550822555 33.0 31.0 34.0 30.0 34.0 3 31.68314065108099 33.0 31.0 34.0 30.0 34.0 4 35.350562329774135 37.0 35.0 37.0 33.0 37.0 5 35.376257607318145 37.0 35.0 37.0 33.0 37.0 6 35.40009072081267 37.0 35.0 37.0 33.0 37.0 7 35.337668435324616 37.0 35.0 37.0 33.0 37.0 8 35.30375755260713 37.0 35.0 37.0 33.0 37.0 9 36.82057870328923 39.0 37.0 39.0 33.0 39.0 10-11 36.69760475170151 39.0 37.0 39.0 32.0 39.0 12-13 36.60466565600498 39.0 37.0 39.0 32.0 39.0 14-15 37.67288581711401 40.0 37.0 41.0 32.0 41.0 16-17 37.60854864605156 40.0 37.0 41.0 32.0 41.0 18-19 37.66337803720747 40.0 37.0 41.0 32.0 41.0 20-21 37.58652447770874 40.0 37.0 41.0 32.0 41.0 22-23 37.66971307553503 40.0 37.0 41.0 32.0 41.0 24-25 37.6962966610364 40.0 37.0 41.0 32.0 41.0 26-27 37.599898336984054 40.0 37.0 41.0 32.0 41.0 28-29 37.44628173985011 40.0 37.0 41.0 32.0 41.0 30-31 37.150561732926676 39.0 36.0 41.0 31.5 41.0 32-33 36.88295274360826 39.0 35.0 41.0 31.0 41.0 34-35 36.65684056811919 39.0 35.0 41.0 30.5 41.0 36-37 36.423439393125506 39.0 35.0 40.5 30.0 41.0 38-39 36.19507411850605 39.0 35.0 40.0 30.0 41.0 40-41 35.92898808493537 38.0 35.0 40.0 30.0 41.0 42-43 35.604783533376704 38.0 35.0 40.0 28.5 41.0 44-45 35.195959939599035 38.0 35.0 40.0 26.5 41.0 46-47 34.73006827934849 38.0 34.0 40.0 24.0 41.0 48-49 34.41093146002813 38.0 34.0 40.0 23.0 41.0 50-51 33.40580105880738 37.5 33.0 39.5 21.0 40.5 52-53 33.48462371752404 37.5 33.0 39.5 20.0 40.5 54-55 33.902865564090476 38.0 34.0 40.0 20.0 41.0 56-57 33.790821878835985 38.0 34.0 40.0 19.5 41.0 58-59 33.55671045537471 38.0 33.5 40.0 18.5 41.0 60-61 33.271235832333616 37.0 33.0 40.0 16.5 41.0 62-63 32.90445069144777 36.5 33.0 40.0 13.0 41.0 64-65 32.53698862607706 36.0 32.5 39.0 9.5 41.0 66-67 32.08088625878112 35.5 32.0 39.0 7.5 41.0 68-69 31.692927457171223 35.0 31.0 39.0 4.5 40.0 70-71 31.306160858346217 35.0 31.0 38.0 2.0 40.0 72-73 30.92804805019885 35.0 31.0 37.0 2.0 39.5 74-75 30.501992973116003 35.0 30.5 37.0 2.0 39.0 76-77 30.045590192602674 35.0 30.0 36.0 2.0 39.0 78-79 29.572887010808905 34.0 29.0 36.0 2.0 38.5 80-81 29.1168980445288 34.0 29.0 35.0 2.0 37.0 82-83 28.724915496348288 34.0 29.0 35.0 2.0 37.0 84-85 28.366691634785063 34.0 28.5 35.0 2.0 36.0 86-87 28.002565449296817 34.0 27.5 35.0 2.0 36.0 88-89 27.58949727539138 34.0 26.5 35.0 2.0 36.0 90-91 27.174385893709427 34.0 26.0 35.0 2.0 35.0 92-93 26.73897423807449 33.0 25.0 35.0 2.0 35.0 94-95 26.275521694410124 33.0 24.5 35.0 2.0 35.0 96-97 25.715365937120133 33.0 21.5 35.0 2.0 35.0 98-99 25.004720068597667 33.0 10.5 35.0 2.0 35.0 100-101 22.188481938401363 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49492319154838427 1101 2 0.5702723803810521 1101 3 0.5770883603760417 1101 4 0.5655436229701039 1101 5 0.5206137815482492 1101 6 0.4503679473372557 1101 7 0.6350658491253256 1101 8 0.5621373355165957 1101 9 0.8001328695695307 1101 10-11 0.7328672695645153 1101 12-13 0.9225333375370113 1101 14-15 0.8708545850758824 1101 16-17 0.9728625549781 1101 18-19 0.9622069006536904 1101 20-21 0.9137241507758915 1101 22-23 0.7489946432949282 1101 24-25 0.7719811669158219 1101 26-27 0.7383552168884222 1101 28-29 0.6280836367785909 1101 30-31 0.7460249040634821 1101 32-33 0.7261119853298581 1101 34-35 0.561851939876199 1101 36-37 0.4475750754281833 1101 38-39 0.5653688466472033 1101 40-41 0.5874069441890342 1101 42-43 0.7227044260016839 1101 44-45 0.744091934353456 1101 46-47 0.8864076864646151 1101 48-49 1.14987802373345 1101 50-51 0.9387556969681938 1101 52-53 1.2333747475622516 1101 54-55 1.1434879925693835 1101 56-57 1.1961684110715325 1101 58-59 1.201180209084427 1101 60-61 1.1989752863115086 1101 62-63 1.1775474494883866 1101 64-65 1.128902263772538 1101 66-67 1.1039507057204858 1101 68-69 0.7035684348704621 1101 70-71 0.7965005890371089 1101 72-73 0.6844497081783238 1101 74-75 0.6713270163721212 1101 76-77 0.7877440466809382 1101 78-79 0.9177725880863292 1101 80-81 0.7553171827066052 1101 82-83 0.9045873758095304 1101 84-85 0.7119060082124626 1101 86-87 0.7554755087155556 1101 88-89 1.088144112871003 1101 90-91 0.932267308953751 1101 92-93 0.6977203550801931 1101 94-95 0.756801961970762 1101 96-97 0.7084778328650998 1101 98-99 0.6350957349123938 1101 100-101 0.8657858132723781 1102 1 0.21078268073509676 1102 2 0.390751154297039 1102 3 0.4370432740431447 1102 4 0.2827920354522533 1102 5 0.36955309666538483 1102 6 0.4818094713544028 1102 7 0.3424200410807998 1102 8 0.34578569515028335 1102 9 0.3284464260224098 1102 10-11 0.3934943494399974 1102 12-13 0.4079137225049223 1102 14-15 0.3090037333075273 1102 16-17 0.2516628951899875 1102 18-19 0.3628914532172516 1102 20-21 0.35924400610326046 1102 22-23 0.2894699490314636 1102 24-25 0.28187745183502955 1102 26-27 0.17741269783407887 1102 28-29 -0.1200489137370866 1102 30-31 0.2872599062517409 1102 32-33 0.20905886438122678 1102 34-35 0.1868773319043484 1102 36-37 -0.09472845633455762 1102 38-39 0.06499064993536763 1102 40-41 -0.048855762146992276 1102 42-43 0.05529622953757496 1102 44-45 0.09746639038602467 1102 46-47 0.25549340830011147 1102 48-49 0.47228700466672535 1102 50-51 0.45542195075636016 1102 52-53 0.34606085254899455 1102 54-55 0.31484000452633865 1102 56-57 0.2984689701090346 1102 58-59 0.1541219684423254 1102 60-61 0.0173636563497368 1102 62-63 0.2153832229336956 1102 64-65 0.18025402800240187 1102 66-67 0.1807440435129095 1102 68-69 0.2653449685076694 1102 70-71 0.4096660459697503 1102 72-73 0.06648206133808188 1102 74-75 0.10903727475148983 1102 76-77 0.2812961230469462 1102 78-79 0.14903450995320355 1102 80-81 0.16974109332666742 1102 82-83 0.3226446172856541 1102 84-85 0.06562026325577364 1102 86-87 0.2087500481495823 1102 88-89 0.3014309560845163 1102 90-91 0.26752094022021566 1102 92-93 0.21457157843921237 1102 94-95 0.15276772608501332 1102 96-97 0.17686744153885314 1102 98-99 -0.07751605194320277 1102 100-101 0.16689686933302283 1103 1 0.3322407908378153 1103 2 0.26680835297336003 1103 3 0.36110054157132154 1103 4 0.5788033615819614 1103 5 0.527516458873464 1103 6 0.48815503019476125 1103 7 0.42061336847565656 1103 8 0.4817922016503289 1103 9 0.31807475511705263 1103 10-11 0.5539686526377423 1103 12-13 0.10351925302996534 1103 14-15 0.3392722985640191 1103 16-17 0.49765636287050086 1103 18-19 0.5591235355984949 1103 20-21 0.5570054602543806 1103 22-23 0.29197142289142874 1103 24-25 0.4353297267432623 1103 26-27 0.2144623098969305 1103 28-29 0.3129332751493621 1103 30-31 0.3031750119178156 1103 32-33 0.13203128491112892 1103 34-35 0.4846155485553041 1103 36-37 0.41209734417580535 1103 38-39 0.19810580896162833 1103 40-41 0.08892324922646822 1103 42-43 0.38250648157838185 1103 44-45 0.55004295579743 1103 46-47 0.7406584160674541 1103 48-49 0.7743736461164019 1103 50-51 0.6030368795592338 1103 52-53 0.5213077509882069 1103 54-55 0.6463556489581705 1103 56-57 0.6387899058057016 1103 58-59 0.5437636375833534 1103 60-61 0.5856457596016966 1103 62-63 0.7628475383096003 1103 64-65 0.4941414466173555 1103 66-67 0.6918266097935728 1103 68-69 0.5832283765022339 1103 70-71 0.5391918718692352 1103 72-73 0.3945499492644764 1103 74-75 0.4680596645132198 1103 76-77 0.4399772658155676 1103 78-79 0.4303154126009865 1103 80-81 0.5065226134894978 1103 82-83 0.29828741387576585 1103 84-85 0.42312920323847436 1103 86-87 0.5719328110883524 1103 88-89 0.445782737411033 1103 90-91 0.6278579700373719 1103 92-93 0.5123885443962735 1103 94-95 0.3569225050490523 1103 96-97 0.6487836316216047 1103 98-99 0.6333573768360061 1103 100-101 0.7703347283846753 1104 1 0.3529324202893207 1104 2 0.4657628051397644 1104 3 0.2982434742140825 1104 4 0.44570521044876443 1104 5 0.3541026174761157 1104 6 0.42732651941778244 1104 7 0.47908829558679145 1104 8 0.527095163889058 1104 9 0.42777007525551625 1104 10-11 0.7189066860917421 1104 12-13 0.6639029435407551 1104 14-15 0.36793722118290617 1104 16-17 0.3747353470947914 1104 18-19 0.32492346337024713 1104 20-21 0.20000020168608756 1104 22-23 0.4224653935949476 1104 24-25 0.5084313131220668 1104 26-27 0.3999536821454228 1104 28-29 0.42904369627524375 1104 30-31 0.39520259958264603 1104 32-33 0.2816754286981862 1104 34-35 0.4174221442268973 1104 36-37 0.29969126291022263 1104 38-39 0.28499682359225176 1104 40-41 0.15459589674384233 1104 42-43 0.2080770406425927 1104 44-45 0.13381186065874573 1104 46-47 0.1647522599311415 1104 48-49 0.12649601132704902 1104 50-51 0.24237704815052297 1104 52-53 0.21411355391081344 1104 54-55 -0.10976657531933398 1104 56-57 0.01365315660505928 1104 58-59 0.013218138195526308 1104 60-61 -0.10884463492304519 1104 62-63 0.0633857267900737 1104 64-65 -0.024149474645049906 1104 66-67 -0.13390910531006028 1104 68-69 -0.062402695734817826 1104 70-71 -0.03099548114188977 1104 72-73 -0.27975089016861787 1104 74-75 -0.22558997566682137 1104 76-77 -0.2194656958946588 1104 78-79 -0.1723825764590785 1104 80-81 0.11855434106571394 1104 82-83 -0.14587575148286547 1104 84-85 0.07910256687243589 1104 86-87 0.01374503527776838 1104 88-89 -0.07438567770757132 1104 90-91 -0.049661430292058384 1104 92-93 -0.4469460749520877 1104 94-95 -0.3921838906736248 1104 96-97 -0.36883653591268484 1104 98-99 -0.1683118002024564 1104 100-101 -0.16107134407258528 1105 1 0.2644001944214338 1105 2 0.15281880262643455 1105 3 0.3310076606735173 1105 4 0.29730313412754583 1105 5 0.09545466193946339 1105 6 0.146373642590369 1105 7 0.38141436132519857 1105 8 0.37493809488367447 1105 9 0.59759458340001 1105 10-11 0.30641654791154593 1105 12-13 0.3535305153356916 1105 14-15 0.20887378960496505 1105 16-17 0.35802099658057074 1105 18-19 0.274847481027102 1105 20-21 0.24998308139041825 1105 22-23 -0.0520672841075438 1105 24-25 0.21909964004558447 1105 26-27 0.02258309481338472 1105 28-29 0.07958669204459312 1105 30-31 0.017001148416966316 1105 32-33 -0.1911496318847199 1105 34-35 0.07940043851595391 1105 36-37 0.2024546021797704 1105 38-39 0.12778441367237292 1105 40-41 -0.021155851909036016 1105 42-43 0.1890761599181019 1105 44-45 0.12191943259487203 1105 46-47 0.12808261633333728 1105 48-49 0.2337105392216614 1105 50-51 0.16223420840531588 1105 52-53 0.31052508698916625 1105 54-55 0.06973531232564056 1105 56-57 0.05157593524669579 1105 58-59 -0.09346777850805665 1105 60-61 -0.146100692771995 1105 62-63 0.024609139588648077 1105 64-65 -0.001158417793313049 1105 66-67 0.03986851440405559 1105 68-69 -0.04895840994237233 1105 70-71 -0.16904948596734215 1105 72-73 -0.27797179140216244 1105 74-75 -0.13319994709181415 1105 76-77 -0.18849649301209226 1105 78-79 -0.16991778337638408 1105 80-81 -0.022500569193930176 1105 82-83 -0.17541355644916123 1105 84-85 -0.11029997010828652 1105 86-87 -0.17401607129342622 1105 88-89 -0.319975436593797 1105 90-91 -0.36934676984753523 1105 92-93 -0.3076477599774776 1105 94-95 -0.011285551695301876 1105 96-97 -0.019075072815745386 1105 98-99 0.2247578335114646 1105 100-101 0.43990927822368775 1106 1 0.29338206512506915 1106 2 0.24103852824427463 1106 3 0.29514628635102724 1106 4 0.324826468492347 1106 5 0.2470608695410519 1106 6 0.4498635786896301 1106 7 0.47024853416662893 1106 8 0.5777659707493754 1106 9 0.5087054383683025 1106 10-11 0.5895051515558478 1106 12-13 0.5031648172731096 1106 14-15 0.4681816010645363 1106 16-17 0.4548997288154695 1106 18-19 0.3438823555619379 1106 20-21 0.130775886772021 1106 22-23 0.5639541834194048 1106 24-25 0.4129874418052282 1106 26-27 0.3813471249133187 1106 28-29 0.24545463643046617 1106 30-31 0.39091711012439845 1106 32-33 0.36946518773113013 1106 34-35 0.40215812730775014 1106 36-37 0.4005983154448245 1106 38-39 0.25606689625195145 1106 40-41 0.46684203432158 1106 42-43 0.4803077409094243 1106 44-45 0.31517904408335795 1106 46-47 0.3859589342251013 1106 48-49 0.3955331510568243 1106 50-51 0.22730975526777542 1106 52-53 0.32090818109002583 1106 54-55 0.4697346916718814 1106 56-57 0.2241651165423164 1106 58-59 0.3587928641523206 1106 60-61 0.5199596542798517 1106 62-63 0.5046054688649733 1106 64-65 0.34144822160596533 1106 66-67 0.3425684469301231 1106 68-69 0.32212648396573584 1106 70-71 0.21051974485645175 1106 72-73 0.09126887418693386 1106 74-75 0.1652529404111469 1106 76-77 0.155683946995957 1106 78-79 0.38215728380938785 1106 80-81 0.4219766319768006 1106 82-83 0.31846812743768993 1106 84-85 0.34294972865297524 1106 86-87 0.4825008375316493 1106 88-89 0.3496452464267996 1106 90-91 0.43270502282219425 1106 92-93 0.4959915763485867 1106 94-95 0.36087129992322886 1106 96-97 0.09565676486024444 1106 98-99 0.23968854638550852 1106 100-101 -0.20750175327626152 1107 1 0.23961266280332083 1107 2 0.22511963750534392 1107 3 0.28525801010166774 1107 4 0.2720755818739846 1107 5 0.2110560264496968 1107 6 0.34135722883887354 1107 7 0.43704885680092787 1107 8 0.4246550714688624 1107 9 0.5549938026088341 1107 10-11 0.4879692406057714 1107 12-13 0.4203511743491575 1107 14-15 0.21107032263507364 1107 16-17 0.13937536760096947 1107 18-19 0.23201731828194028 1107 20-21 0.45097477901323657 1107 22-23 0.33639998032075624 1107 24-25 0.2833475617736809 1107 26-27 0.36240174918922463 1107 28-29 0.28588318205442675 1107 30-31 0.3191944683146417 1107 32-33 0.26986123742825896 1107 34-35 0.3611903674878718 1107 36-37 0.3286758156370837 1107 38-39 0.2428078606137376 1107 40-41 0.41759738130547674 1107 42-43 0.3842708311164742 1107 44-45 0.6811869990576227 1107 46-47 0.5054360127035693 1107 48-49 0.5035132528905066 1107 50-51 0.37589948211957136 1107 52-53 0.39906786002955386 1107 54-55 0.5071613433395115 1107 56-57 0.6187268328709408 1107 58-59 0.6630013281161808 1107 60-61 0.6222628053295765 1107 62-63 0.6934824547023268 1107 64-65 0.6051275402824459 1107 66-67 0.6548977628345298 1107 68-69 0.735192278828265 1107 70-71 0.49509026030230174 1107 72-73 0.6031378460315437 1107 74-75 0.6822301396092101 1107 76-77 0.5297573123184911 1107 78-79 0.4979023383299328 1107 80-81 0.6031221983484336 1107 82-83 0.6770893844595456 1107 84-85 0.4141693721456541 1107 86-87 0.625357724897583 1107 88-89 0.39407005779125015 1107 90-91 0.3006212416340581 1107 92-93 0.44659893004985207 1107 94-95 0.4247798069884574 1107 96-97 0.47526758276680425 1107 98-99 0.640826798043836 1107 100-101 0.4630933675059268 1108 1 0.2787196640772507 1108 2 0.3541322966353313 1108 3 0.24842448523329352 1108 4 0.3825592301265601 1108 5 0.4401925152114927 1108 6 0.3444930583637813 1108 7 0.3623974060343471 1108 8 0.3493978354531464 1108 9 0.4645536283564837 1108 10-11 0.3962221737644924 1108 12-13 0.19272113096423737 1108 14-15 0.4946713595968859 1108 16-17 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-1.5459436084856861 2316 72-73 -1.4657676191853177 2316 74-75 -1.3729767190440718 2316 76-77 -1.6447205218779466 2316 78-79 -2.2452572494900593 2316 80-81 -2.3318637709193304 2316 82-83 -2.3507472211816562 2316 84-85 -2.3600511363565317 2316 86-87 -2.0124635306866026 2316 88-89 -2.400180668984614 2316 90-91 -2.6797695767832543 2316 92-93 -3.262257639843387 2316 94-95 -3.110084210779984 2316 96-97 -3.5333476080534467 2316 98-99 -4.2434460474987965 2316 100-101 -4.933205983845177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1677.0 3 607.0 4 643.0 5 735.0 6 815.0 7 934.0 8 1269.0 9 1572.0 10 2262.0 11 3170.0 12 4450.0 13 5901.0 14 7110.0 15 9461.0 16 12509.0 17 17359.0 18 12777.0 19 7284.0 20 6803.0 21 7181.0 22 7856.0 23 8671.0 24 9921.0 25 11224.0 26 12898.0 27 15227.0 28 18171.0 29 21267.0 30 26232.0 31 32391.0 32 40899.0 33 52802.0 34 70885.0 35 99897.0 36 136962.0 37 176546.0 38 138960.0 39 19952.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.302469928002296 18.782973245348302 14.441323914040497 20.4732329126089 2 15.493193150570454 21.022738577983294 40.41032110183015 23.0737471696161 3 19.0831743176586 28.02151337820925 30.148675510524882 22.746636793607273 4 12.060913463164786 17.035453259284168 37.86476542585897 33.038867851692075 5 12.379861630050936 39.25971181716778 33.102318736461264 15.258107816320013 6 29.323747508252744 36.9586700861948 18.068862290347397 15.648720115205055 7 27.364575830770026 35.2600247509978 20.531290911767865 16.84410850646431 8 27.271379489103172 32.70641193134046 19.837972890010818 20.18423568954555 9 28.403485605508028 16.40208849744212 20.583063746538325 34.611362150511525 10-11 24.673920506959448 27.42585529006571 26.32937039067841 21.570853812296434 12-13 27.002164690617402 25.48867231479607 27.289479981711324 20.21968301287521 14-15 23.76044776119403 25.686517412935324 27.560398009950248 22.9926368159204 16-17 20.710404779282083 29.601857504120627 27.70529883199528 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26.76466374993285 31.252574027539858 21.311107662494504 44-45 20.695170967611975 26.841022279233258 31.073594137258542 21.390212615896232 46-47 21.29192445255057 27.126792605609108 30.26911528232808 21.312167659512244 48-49 21.262060736523733 27.489949282610304 29.55231719830315 21.69567278256281 50-51 21.34752213995986 27.78878938376179 29.3244732044934 21.53921527178495 52-53 21.487174564299274 27.966318775904664 28.954532138073326 21.591974521722737 54-55 20.882109653893924 27.78209687070526 29.248880724891357 22.086912750509462 56-57 21.53569229453094 28.22616344017993 28.407656427656963 21.830487837632166 58-59 21.667697116415287 27.32066319513701 28.813613277432054 22.198026411015647 60-61 21.30864354730728 27.698764582237 29.432219146140206 21.560372724315517 62-63 21.733291686478765 27.73904332930791 29.31358290940416 21.214082074809156 64-65 21.373096230834236 27.928314178551055 29.196175575750132 21.50241401486458 66-67 21.372610763138038 28.090754561830018 28.77928856295328 21.75734611207867 68-69 21.33715809743694 27.98553025063318 29.141070104641965 21.53624154728792 70-71 21.312533497088584 28.036683697411746 28.897891667740726 21.75289113775894 72-73 21.308975140217136 28.14062752608474 28.686274265958637 21.864123067739488 74-75 20.986904951440856 28.829157379398186 28.50123387995542 21.68270378920554 76-77 21.16866033706993 28.30030228434629 28.881488218074576 21.649549160509206 78-79 21.093784991335834 28.274591848617465 28.798622094070648 21.833001065976056 80-81 20.95482001926365 28.709957614990806 28.44917656604271 21.88604579970283 82-83 20.97363870470124 28.48413221701106 28.51020738215792 22.032021696129778 84-85 20.88112713275011 28.30333131363179 28.364867540411442 22.45067401320666 86-87 21.063547180323955 28.381770605324952 28.28909141560871 22.265590798742387 88-89 21.145033003201817 28.028046254231114 28.096550036117463 22.730370706449605 90-91 20.708971004873156 28.003528733905004 28.674097640246277 22.613402620975567 92-93 20.945634334094347 28.18186928897329 28.570668102129464 22.3018282748029 94-95 20.568042309934597 27.797432035290115 28.70371107312909 22.930814581646196 96-97 20.667672216092306 27.508160968829625 28.739643951928635 23.08452286314943 98-99 20.548882499475013 27.887502525410856 28.523109873037768 23.040505102076363 100-101 20.459991966988003 27.815921589043253 28.7497716763164 22.974314767652345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 710.0 1 576.0 2 769.0 3 1026.5 4 846.0 5 831.5 6 1276.0 7 2387.0 8 3854.0 9 4450.0 10 3665.5 11 2814.0 12 2699.0 13 2783.5 14 2843.5 15 2831.5 16 2763.0 17 2770.0 18 2888.5 19 3041.5 20 3194.0 21 3492.0 22 3896.5 23 4375.5 24 4943.5 25 5722.0 26 6848.5 27 8180.0 28 9263.5 29 10470.5 30 12530.0 31 14835.5 32 17826.0 33 20584.5 34 22558.0 35 24053.0 36 26823.0 37 29844.5 38 30003.5 39 29367.5 40 29031.0 41 29473.5 42 29963.5 43 30731.5 44 32346.5 45 34509.5 46 34687.0 47 35280.5 48 42540.0 49 41640.0 50 33392.5 51 29831.5 52 27648.0 53 27463.5 54 27274.5 55 25501.0 56 23324.0 57 21116.0 58 19760.5 59 18423.0 60 16078.0 61 13850.5 62 11723.5 63 8783.0 64 6014.5 65 4568.0 66 3459.0 67 2689.0 68 2110.5 69 1542.0 70 1211.0 71 1029.0 72 869.5 73 809.5 74 661.5 75 512.5 76 406.5 77 254.5 78 155.5 79 99.0 80 69.5 81 50.5 82 35.0 83 19.5 84 11.5 85 10.5 86 8.0 87 7.0 88 5.5 89 2.5 90 1.0 91 1.0 92 3.0 93 3.0 94 2.0 95 2.5 96 2.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011041677857556388 2 0.011737999884609492 3 0.013926440541062108 4 0.018303321853967346 5 0.029344999711523732 6 0.04625567751138486 7 0.006963220270531054 8 0.026360762452724708 9 0.034716626777361974 10-11 0.038695609789094004 12-13 0.028698414972117274 14-15 0.028051830232710823 16-17 0.02760419464389097 18-19 0.022431516728639328 20-21 0.053964957096615675 22-23 0.012235372761075995 24-25 0.002785288108212422 26-27 0.001143957615872959 28-29 0.0 30-31 0.002735550820565772 32-33 0.002685813532919121 34-35 0.0 36-37 2.4868643823325196E-4 38-39 9.947457529330078E-5 40-41 0.0010942203282263087 42-43 0.004774779614078437 44-45 0.0018402796429260646 46-47 6.465847394064551E-4 48-49 5.471101641131543E-4 50-51 0.002934499971152373 52-53 0.0033323982723255765 54-55 0.005520838928778194 56-57 8.952711776397071E-4 58-59 0.005719788079364795 60-61 0.003481610135265527 62-63 3.978983011732031E-4 64-65 6.465847394064551E-4 66-67 0.017457787963974288 68-69 0.04247564365023943 70-71 0.052771262193096066 72-73 0.014374076129881964 74-75 0.03203081324444285 76-77 0.02665918617860461 78-79 0.05660103334188815 80-81 0.07952992294699397 82-83 0.049190177482537234 84-85 0.018203847278674044 86-87 0.02009386420924676 88-89 0.022680203166872578 90-91 0.038695609789094004 92-93 0.02621155058978476 94-95 0.0127327456375425 96-97 0.01919859303160705 98-99 0.049239914770183885 100-101 0.06739402476121129 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1005282.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.09478952915957 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0041434633765 34.85209560693446 2 13.563513573947445 12.775416340819076 3 4.984765129286366 7.04269393848106 4 2.309169054128476 4.349993223657163 5 1.303259528563689 3.068836659978933 6 0.7853087496880203 2.2190370169178864 7 0.5440013198061632 1.7933739361899397 8 0.3531207296581259 1.3304117153306156 9 0.28614268583468233 1.2128246608223514 >10 1.6314877258293052 14.48605335092694 >50 0.14661561361921693 4.791247608075519 >100 0.08147101130780163 6.9396209730647165 >500 0.004243281790406002 1.2385863966332693 >1k 0.002333804984723301 2.076800439982454 >5k 2.1216408952030006E-4 0.7835587707646094 >10k+ 2.1216408952030006E-4 1.0394493614210956 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10403 1.034834006776208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7842 0.7800796194500648 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4974 0.49478653750887813 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2299 0.2286920485992985 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2151 0.21396981145589 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1888 0.18780799815375188 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1736 0.17268786270917016 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1561 0.15527981203284252 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1426 0.14185074436824693 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.12872010042953122 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.12275162591193318 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1137 0.11310259210848299 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1085 0.10792991419323135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.947457529330078E-5 0.0 9 0.0 0.0 0.0 9.947457529330078E-5 0.0 10-11 0.0 0.0 0.0 1.4921186293995116E-4 0.0 12-13 0.0 0.0 0.0 1.9894915058660156E-4 0.0 14-15 0.0 0.0 0.0 1.9894915058660156E-4 0.0 16-17 0.0 0.0 0.0 2.4868643823325196E-4 0.0 18-19 0.0 0.0 0.0 2.984237258799024E-4 0.0 20-21 0.0 0.0 0.0 4.476355888198535E-4 0.0 22-23 0.0 0.0 0.0 7.460593146997559E-4 0.0 24-25 0.0 0.0 0.0 0.001143957615872959 0.0 26-27 0.0 0.0 0.0 0.0019894915058660157 0.0 28-29 0.0 0.0 0.0 0.00378003386114543 0.0 30-31 0.0 0.0 0.0 0.009251135502276974 0.0 32-33 0.0 0.0 0.0 0.014573025280468565 0.0 34-35 0.0 0.0 0.0 0.0191488557439604 0.0 36-37 0.0 0.0 0.0 0.022332042153346027 0.0 38-39 0.0 0.0 0.0 0.02561470313802495 0.0 40-41 0.0 0.0 0.0 0.030886855628569895 0.0 42-43 0.0 0.0 0.0 0.03670611828322799 0.0 44-45 0.0 0.0 0.0 0.04252538093788608 0.0 46-47 0.0 0.0 0.0 0.05127914356369656 0.0 48-49 0.0 0.0 0.0 0.06087844007950008 0.0 50-51 0.0 0.0 0.0 0.0742080331688024 0.0 52-53 0.0 0.0 0.0 0.10221012611386655 0.0 54-55 0.0 0.0 0.0 0.13409172749536946 0.0 56-57 0.0 0.0 0.0 0.17442866777680294 0.0 58-59 0.0 0.0 0.0 0.23655054004746928 0.0 60-61 0.0 0.0 0.0 0.32438659003145387 0.0 62-63 0.0 0.0 0.0 0.3867571487403535 0.0 64-65 0.0 0.0 0.0 0.44519846172516764 0.0 66-67 0.0 0.0 0.0 0.5578534182448308 0.0 68-69 0.0 0.0 0.0 0.7002015354895443 0.0 70-71 0.0 0.0 0.0 0.812757017433914 0.0 72-73 0.0 0.0 0.0 0.9113860588372218 0.0 74-75 0.0 0.0 0.0 1.1050133196456318 0.0 76-77 0.0 0.0 0.0 1.2473116996026987 0.0 78-79 0.0 0.0 0.0 1.361757198477641 0.0 80-81 0.0 0.0 0.0 1.4823203837331216 0.0 82-83 0.0 0.0 0.0 1.686840110536148 0.0 84-85 0.0 0.0 0.0 1.889171396682722 0.0 86-87 0.0 0.0 0.0 2.1837156141261853 0.0 88-89 0.0 0.0 0.0 2.57803283058883 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 25 0.0050213938 56.999138 2 GTATCAA 12880 0.0 27.769377 1 GTACATG 9255 0.0 27.714327 1 TACATGG 9385 0.0 26.824314 2 ACATGGG 9355 0.0 25.691753 3 GTCTTAG 260 0.0 25.576534 1 GAGTACT 7160 0.0 24.3829 12-13 GGTATCA 9225 0.0 24.200176 1 CATGGGA 6300 0.0 23.825033 4 CATGGGG 2470 0.0 23.268877 4 ATCAACG 15360 0.0 23.193007 3 GTCCTAT 185 2.5413465E-7 23.107758 1 GTATAGT 185 2.5413465E-7 23.107758 1 TCAACGC 15440 0.0 23.042072 4 TATCAAC 15525 0.0 23.038298 2 CAACGCA 15580 0.0 22.926481 5 AGTACTT 7295 0.0 22.759512 12-13 GTACTTT 7720 0.0 22.674599 14-15 AGGGTCG 105 0.0021962249 22.618704 5 AACGCAG 15825 0.0 22.543764 6 >>END_MODULE