FastQCFastQC Report
Thu 26 May 2016
SRR936707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005282
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113251.1265495651966315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96570.9606259736074056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62740.6241034853901691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31670.3150359799538836No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27930.2778324887941891No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.23655054004746928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.19238382861724374No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.17527420166679597No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17280.17189206610682375No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16440.16353620178218647No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11680.11618630394257531No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.10802938876852465No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.10484620235913904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG500.001613509538.010592
TATCACG550.002575950234.555082
ATCACGC650.00584003429.2345523
GGTATCA103600.028.208061
GTATCAA144500.027.3598251
GTACATG90600.025.8569071
TACATGG91400.025.3161352
AGCCCGA950.001227657324.9957739
CCCTATA2101.2605597E-924.8878862
CATGGGG24100.024.6352144
GAGTACT95200.024.34764912-13
ACATGGG89200.024.2323953
AGTACTT97300.023.08992212-13
GTACTTT101700.022.60525114-15
TAAGGTG4650.022.4715525
AGGTAAG3600.022.4368082
ATCAACG176500.022.343
TATCAAC176450.022.3227392
TCAACGC177800.022.1722474
GTAAGGT4100.022.010664
Produced by FastQC (version 0.11.5)