##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936707_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1005282 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.958858310404445 31.0 31.0 34.0 30.0 34.0 2 32.04735487156837 33.0 31.0 34.0 30.0 34.0 3 32.13989010048922 33.0 31.0 34.0 30.0 34.0 4 35.8989149313327 37.0 35.0 37.0 35.0 37.0 5 35.78687273819684 37.0 35.0 37.0 35.0 37.0 6 35.865744139455394 37.0 35.0 37.0 35.0 37.0 7 35.79108847069777 37.0 35.0 37.0 33.0 37.0 8 35.76886187159424 37.0 35.0 37.0 33.0 37.0 9 37.35598070989036 39.0 37.0 39.0 34.0 39.0 10-11 37.26659534339618 39.0 37.0 39.0 34.0 39.0 12-13 37.13268913598374 39.0 37.0 39.0 33.0 39.0 14-15 38.151139680209134 40.0 37.5 41.0 33.0 41.0 16-17 38.15461631661563 40.0 37.5 41.0 33.0 41.0 18-19 38.37151565431391 40.0 38.0 41.0 33.5 41.0 20-21 38.425990418608905 40.0 38.0 41.0 34.0 41.0 22-23 38.31633312841571 40.0 38.0 41.0 34.0 41.0 24-25 38.18877887000861 40.0 37.5 41.0 33.0 41.0 26-27 37.95732739669068 40.0 37.0 41.0 33.0 41.0 28-29 37.908847965048615 40.0 37.0 41.0 33.0 41.0 30-31 37.671106216962 39.5 36.5 41.0 33.0 41.0 32-33 37.360162621035684 39.0 35.5 41.0 32.5 41.0 34-35 37.11842100027654 39.0 35.0 41.0 32.0 41.0 36-37 36.86687914435949 39.0 35.0 41.0 31.0 41.0 38-39 36.6128379897382 39.0 35.0 41.0 31.0 41.0 40-41 36.31336430971608 39.0 35.0 40.5 30.0 41.0 42-43 35.944520542494544 38.5 35.0 40.0 30.0 41.0 44-45 35.47126826104516 38.0 35.0 40.0 28.0 41.0 46-47 34.84124454630641 38.0 34.5 40.0 25.0 41.0 48-49 34.24124424788268 38.0 34.0 40.0 22.5 41.0 50-51 33.87190609202194 38.0 33.0 40.0 20.0 41.0 52-53 33.58083701886635 38.0 33.0 40.0 19.5 41.0 54-55 33.23692108284044 37.5 33.0 40.0 17.0 41.0 56-57 32.89866574752159 37.0 32.5 40.0 14.0 41.0 58-59 32.54496549226983 36.5 32.0 39.5 10.5 41.0 60-61 32.16542074761112 36.0 31.0 39.0 8.0 40.0 62-63 31.8394186904769 36.0 31.0 39.0 7.0 40.0 64-65 31.531976102228032 35.0 31.0 39.0 4.0 40.0 66-67 31.085621745937956 35.0 30.5 38.0 2.0 40.0 68-69 30.642995199356996 35.0 30.0 38.0 2.0 40.0 70-71 30.150107631490467 34.5 29.0 37.0 2.0 39.0 72-73 29.61805493383946 34.0 29.0 36.5 2.0 39.0 74-75 29.09560401956864 34.0 28.0 36.0 2.0 39.0 76-77 27.733095788047535 32.0 26.5 34.5 2.0 36.5 78-79 28.464529853314794 33.5 28.0 35.0 2.0 37.0 80-81 28.29353007414835 34.0 27.5 35.0 2.0 37.0 82-83 27.8986254603186 33.5 27.0 35.0 2.0 36.0 84-85 27.45528369154128 33.0 26.0 35.0 2.0 36.0 86-87 27.029528032930063 33.0 26.0 35.0 2.0 35.5 88-89 26.525294892378458 32.5 25.0 35.0 2.0 35.0 90-91 25.94637524595089 32.0 23.5 34.0 2.0 35.0 92-93 25.39705276728321 32.0 21.5 34.0 2.0 35.0 94-95 24.699216737194142 31.0 18.5 34.0 2.0 35.0 96-97 23.907600056501558 31.0 4.5 34.0 2.0 35.0 98-99 22.87297594107922 31.0 2.0 34.0 2.0 35.0 100-101 19.299629357732456 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.348646044211538 1101 2 0.44429545734999465 1101 3 0.30782372064888364 1101 4 0.286926745925534 1101 5 0.3397605949870055 1101 6 0.3068050730048597 1101 7 0.3410780957534456 1101 8 0.23264919600740086 1101 9 0.5142419608339779 1101 10-11 0.4111659307373543 1101 12-13 0.37828283499932525 1101 14-15 0.35490801513615367 1101 16-17 0.4387698697971487 1101 18-19 0.5388787521512626 1101 20-21 0.39277081267081115 1101 22-23 0.40596325930665955 1101 24-25 0.3552213726192903 1101 26-27 0.37379948965097753 1101 28-29 0.5627443744092133 1101 30-31 0.5224399539987203 1101 32-33 0.6090524996273743 1101 34-35 0.7243128829649592 1101 36-37 0.7326636862459566 1101 38-39 0.770698908827633 1101 40-41 0.8320294338760448 1101 42-43 0.7488622572399848 1101 44-45 0.7051625441050788 1101 46-47 0.6175207235269156 1101 48-49 0.6229769171202193 1101 50-51 0.580090791530921 1101 52-53 0.7024903113957706 1101 54-55 0.6073569120216504 1101 56-57 0.6269867094153057 1101 58-59 0.5213673909313528 1101 60-61 0.48826817671711353 1101 62-63 0.5016558818094694 1101 64-65 0.5339586981500304 1101 66-67 0.524738474991846 1101 68-69 0.5456956490627007 1101 70-71 0.4519460427775499 1101 72-73 0.41711601811478616 1101 74-75 0.6205097409192604 1101 76-77 0.6110189898192004 1101 78-79 0.6900612762098781 1101 80-81 0.8643424976698242 1101 82-83 0.845790588243073 1101 84-85 0.6786377163269179 1101 86-87 0.6277376341344798 1101 88-89 0.7123060221595097 1101 90-91 0.9164132731033057 1101 92-93 0.8405413498815264 1101 94-95 0.5556945084722216 1101 96-97 0.5988874497761003 1101 98-99 0.018638278223768623 1101 100-101 0.1434311351469617 1102 1 0.28683400302277917 1102 2 0.40126608517304874 1102 3 0.30253805001032674 1102 4 0.12308829702750046 1102 5 0.01636359578799329 1102 6 0.13452903895064594 1102 7 0.20208832688282996 1102 8 0.12806376731564484 1102 9 0.3485158314048249 1102 10-11 0.223648486620462 1102 12-13 0.27176224474821 1102 14-15 0.4869709213053923 1102 16-17 0.5193493034929944 1102 18-19 0.3966635763888817 1102 20-21 0.23938603467558295 1102 22-23 0.35427830212216094 1102 24-25 0.09843943027364332 1102 26-27 0.28419618331989227 1102 28-29 0.2939278493598749 1102 30-31 0.44906607599606474 1102 32-33 0.5156139648506226 1102 34-35 0.48436523595956515 1102 36-37 0.6009347987883871 1102 38-39 0.4499009798262179 1102 40-41 0.7052410506257019 1102 42-43 0.8257418866402375 1102 44-45 0.8365871401034539 1102 46-47 0.8560526101339647 1102 48-49 0.6979812523445403 1102 50-51 0.5007103570175815 1102 52-53 0.4317460566001827 1102 54-55 0.44313448611270445 1102 56-57 0.6383107282095324 1102 58-59 0.7878172963821655 1102 60-61 0.4016214933701576 1102 62-63 0.31697367439582536 1102 64-65 0.48174443072036155 1102 66-67 0.3710898676310457 1102 68-69 0.5248878109771375 1102 70-71 0.2916930308288457 1102 72-73 0.24825944385032983 1102 74-75 0.3288226367665281 1102 76-77 0.319072185085961 1102 78-79 0.2459241510003416 1102 80-81 0.35159446090712265 1102 82-83 0.3532257043857996 1102 84-85 0.3164648193235884 1102 86-87 0.4476301205714215 1102 88-89 0.3503114886709362 1102 90-91 0.2937218832716759 1102 92-93 0.5340038316116491 1102 94-95 0.509798107947038 1102 96-97 0.48378228962052816 1102 98-99 0.5844317068494647 1102 100-101 0.5120849860647887 1103 1 0.24740254719339205 1103 2 0.288845641336799 1103 3 0.24162653755029595 1103 4 0.16927670151492435 1103 5 0.12863256554911828 1103 6 0.15395644973370537 1103 7 0.10501668222723737 1103 8 0.10378230094332253 1103 9 0.06198970858172714 1103 10-11 0.19136514403516003 1103 12-13 0.2810933920352383 1103 14-15 0.30937445340541814 1103 16-17 0.3245394421864418 1103 18-19 0.3143640966506922 1103 20-21 0.30441273628456145 1103 22-23 0.20554453071891743 1103 24-25 0.2621050168268724 1103 26-27 0.1405613219057642 1103 28-29 0.2337823137570112 1103 30-31 0.41602579335920353 1103 32-33 0.44217012429430014 1103 34-35 0.391017615866879 1103 36-37 0.5078191233732241 1103 38-39 0.270794074415754 1103 40-41 0.4021220617151471 1103 42-43 0.5566658354660987 1103 44-45 0.70124806624694 1103 46-47 0.6364840530536284 1103 48-49 0.9099963309001922 1103 50-51 1.1241143925967734 1103 52-53 0.911055214326872 1103 54-55 0.9383428275146102 1103 56-57 1.0707946018147538 1103 58-59 1.0250915376132426 1103 60-61 0.7658852303905093 1103 62-63 1.0618005335034155 1103 64-65 1.0077437508083165 1103 66-67 1.2949199240465354 1103 68-69 1.4533279292301948 1103 70-71 1.0643937017040805 1103 72-73 1.1907995608124118 1103 74-75 1.423555039739206 1103 76-77 1.26641170647952 1103 78-79 1.1561886712105114 1103 80-81 1.3725774932287642 1103 82-83 1.3115690427539235 1103 84-85 1.4168942827524944 1103 86-87 1.0514402099497282 1103 88-89 1.1594954651236051 1103 90-91 0.8701056979224902 1103 92-93 1.0476597358731503 1103 94-95 0.9589174497233337 1103 96-97 0.9750135759022243 1103 98-99 1.2111328855071122 1103 100-101 0.9250007329137411 1104 1 0.22095644091217892 1104 2 0.33169636670212554 1104 3 0.2337774417245626 1104 4 0.20112142841289682 1104 5 0.12590903235495432 1104 6 0.1961632867533183 1104 7 0.1857969233991028 1104 8 0.23430225619704714 1104 9 0.3796257310957998 1104 10-11 0.30587377178666486 1104 12-13 0.29151407749886005 1104 14-15 0.31975682266789107 1104 16-17 0.2879915117017191 1104 18-19 0.23025342889178546 1104 20-21 0.4181937966303835 1104 22-23 0.3040532817869277 1104 24-25 0.21198742572681084 1104 26-27 0.35860164640434533 1104 28-29 0.246324483599075 1104 30-31 0.3526345012664365 1104 32-33 0.4423731561187694 1104 34-35 0.6218172353473577 1104 36-37 0.3705370192412403 1104 38-39 0.4191345865316052 1104 40-41 0.46996023522147823 1104 42-43 0.4436647520424799 1104 44-45 0.48065905374793516 1104 46-47 0.5065789186826706 1104 48-49 0.44128481225738625 1104 50-51 0.3835084681193237 1104 52-53 0.42233294953108924 1104 54-55 0.1872505955738788 1104 56-57 0.5417738229829041 1104 58-59 0.6742340474565438 1104 60-61 0.3846645731135112 1104 62-63 0.4141924333704097 1104 64-65 0.4266221871549831 1104 66-67 0.3949740952274645 1104 68-69 0.5311375637729121 1104 70-71 0.4802720235425575 1104 72-73 0.6614731332036108 1104 74-75 0.7655108395788979 1104 76-77 0.3893534218121708 1104 78-79 0.39536104126574045 1104 80-81 0.4878757870567725 1104 82-83 0.6416292035938831 1104 84-85 0.8138827174255745 1104 86-87 0.9360282887665967 1104 88-89 0.7123956051759244 1104 90-91 0.6818088750842719 1104 92-93 0.9708846387613974 1104 94-95 1.2187136251498742 1104 96-97 0.9400542263087281 1104 98-99 0.8748566917592555 1104 100-101 0.7968259934198691 1105 1 0.035157108427210204 1105 2 0.1304658233886613 1105 3 0.11625896680755687 1105 4 0.08282989272237273 1105 5 0.09552491998933732 1105 6 0.14080031001044802 1105 7 0.11045085515442565 1105 8 0.1298702175934281 1105 9 0.05803862673788984 1105 10-11 0.11692262816855958 1105 12-13 0.1477755167685828 1105 14-15 0.05430840329477604 1105 16-17 0.1508989791511013 1105 18-19 0.1575663014126576 1105 20-21 -0.028683426139970436 1105 22-23 0.09263704503971582 1105 24-25 0.09733615908967153 1105 26-27 0.11670245847115268 1105 28-29 0.11267233623745909 1105 30-31 -0.019392612339963478 1105 32-33 0.011847580691508597 1105 34-35 -0.13350241116265948 1105 36-37 0.0705523745579768 1105 38-39 0.05464922791435356 1105 40-41 0.17904493479559846 1105 42-43 0.05894082122259903 1105 44-45 -0.04212373558947746 1105 46-47 0.03907835830185746 1105 48-49 -0.11747972013792207 1105 50-51 0.20169842927214887 1105 52-53 0.18853016121736488 1105 54-55 0.23216092581908043 1105 56-57 0.34583909896384313 1105 58-59 0.1916279148607387 1105 60-61 0.15888846775132492 1105 62-63 0.07468902784355436 1105 64-65 0.047679003591873936 1105 66-67 0.3313117462876001 1105 68-69 0.2304773734022838 1105 70-71 0.23311187464893024 1105 72-73 0.28583619643044855 1105 74-75 0.2217208297126625 1105 76-77 -0.1302126934162473 1105 78-79 0.09323900709221888 1105 80-81 0.41461327644483603 1105 82-83 0.03716804283783759 1105 84-85 0.26037561603283166 1105 86-87 0.3915702343734786 1105 88-89 0.2500099537486733 1105 90-91 0.27551381296584054 1105 92-93 0.09345889583764233 1105 94-95 -0.06496349666367607 1105 96-97 -0.08845459593847949 1105 98-99 -0.07396509655269767 1105 100-101 -0.3804851141220631 1106 1 0.06339330796916798 1106 2 0.16564536522205486 1106 3 0.10446211210349077 1106 4 0.12706565957999771 1106 5 0.19612846143969875 1106 6 0.050308991626593524 1106 7 0.1913060805714366 1106 8 0.18527420555983554 1106 9 0.2944119517485504 1106 10-11 0.16331162025345236 1106 12-13 0.1539173290598299 1106 14-15 0.24550035111020918 1106 16-17 0.28668871229657356 1106 18-19 0.26172308564592583 1106 20-21 0.24723795653474667 1106 22-23 0.18524168948047048 1106 24-25 0.20150313977759282 1106 26-27 -0.026944751291196667 1106 28-29 0.03823112411839702 1106 30-31 0.1349706279337326 1106 32-33 0.06246632815901876 1106 34-35 0.1477286644391782 1106 36-37 0.1599123297999796 1106 38-39 0.19678470244405588 1106 40-41 0.21620125885842612 1106 42-43 0.30486246102530146 1106 44-45 0.1471401956491789 1106 46-47 0.10011788242001529 1106 48-49 0.28519512024323035 1106 50-51 0.05620078465316425 1106 52-53 -0.05132769133835069 1106 54-55 6.965287354461225E-4 1106 56-57 0.11673673276570895 1106 58-59 0.07462625771518816 1106 60-61 -0.03522232603823028 1106 62-63 -0.03489980677260718 1106 64-65 -0.160585799221991 1106 66-67 -0.24573133506326528 1106 68-69 -0.030442364539968736 1106 70-71 0.13149533759545662 1106 72-73 0.18471839900651688 1106 74-75 0.07708988373880032 1106 76-77 0.09607855456773606 1106 78-79 0.11063637534294202 1106 80-81 0.04163317194447913 1106 82-83 0.1260115254856906 1106 84-85 0.15039222295418142 1106 86-87 0.12742176690060703 1106 88-89 0.009103196336518238 1106 90-91 0.23523072391887112 1106 92-93 0.1408444805333815 1106 94-95 0.1528185882541706 1106 96-97 0.40297461894338227 1106 98-99 0.3080996057152561 1106 100-101 -0.13292849498269632 1107 1 0.1118304810202595 1107 2 0.1536771350428694 1107 3 0.1097845049626116 1107 4 0.05503451213576227 1107 5 -0.021333136315455192 1107 6 0.11987924774886949 1107 7 0.021307851125513366 1107 8 0.12704890881318676 1107 9 0.12783411085877106 1107 10-11 -0.005246022012357798 1107 12-13 0.08309248335736186 1107 14-15 0.0686309410981707 1107 16-17 0.17137154167776458 1107 18-19 0.1523922656647727 1107 20-21 0.12253729642211653 1107 22-23 0.08114831153155677 1107 24-25 0.16407738232918945 1107 26-27 -0.020008739830885247 1107 28-29 0.09614523257287999 1107 30-31 0.09992170519963395 1107 32-33 0.09361153684296397 1107 34-35 -0.07990870104789138 1107 36-37 0.06879630843171469 1107 38-39 0.03792799749884779 1107 40-41 0.18345942173867513 1107 42-43 0.19902893245409814 1107 44-45 0.3327725070091603 1107 46-47 0.6485975308845582 1107 48-49 0.4001398485526053 1107 50-51 0.22358789394642997 1107 52-53 0.41272246005296864 1107 54-55 0.5230748120849071 1107 56-57 0.48379047712989376 1107 58-59 0.4588248673831359 1107 60-61 0.3106607780470938 1107 62-63 0.14894719589395322 1107 64-65 0.2219102170313363 1107 66-67 0.2078846192385555 1107 68-69 0.4017190348773596 1107 70-71 0.2706658061842191 1107 72-73 0.10719585126862441 1107 74-75 -0.15829857403706526 1107 76-77 -0.3636780031889906 1107 78-79 -0.02268724080808937 1107 80-81 0.1372949567702939 1107 82-83 0.3957384488171236 1107 84-85 0.21235312053567768 1107 86-87 0.44216145074046054 1107 88-89 0.4613863168754797 1107 90-91 0.30271342524785894 1107 92-93 0.24857509520179732 1107 94-95 0.2911091451202559 1107 96-97 0.3122368523095602 1107 98-99 0.5044674660453694 1107 100-101 0.2689068005656985 1108 1 0.03331804015113704 1108 2 0.19213202631332393 1108 3 0.04350447182259387 1108 4 0.11293867334590857 1108 5 0.1530457110890282 1108 6 0.11264189574309569 1108 7 0.06745799678122921 1108 8 0.18735037606069227 1108 9 0.2572948729009781 1108 10-11 0.09887688112436877 1108 12-13 0.1848492605798313 1108 14-15 0.10561858486081377 1108 16-17 0.20106526378268796 1108 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-2.1448314762765897 2316 80-81 -2.024761721422305 2316 82-83 -1.8116513760912731 2316 84-85 -1.709032904717347 2316 86-87 -1.948042620122198 2316 88-89 -2.064432869089657 2316 90-91 -2.1759519458036323 2316 92-93 -2.3000351395462317 2316 94-95 -2.4768359663798982 2316 96-97 -2.5885091971731455 2316 98-99 -2.171018791179371 2316 100-101 -2.0351482301917763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 21.0 5 62.0 6 108.0 7 253.0 8 553.0 9 1025.0 10 1712.0 11 2926.0 12 4367.0 13 5996.0 14 7691.0 15 10766.0 16 14835.0 17 19547.0 18 16138.0 19 9058.0 20 7333.0 21 7327.0 22 8041.0 23 8801.0 24 10321.0 25 12036.0 26 14293.0 27 16999.0 28 20873.0 29 25345.0 30 30892.0 31 37749.0 32 47178.0 33 59148.0 34 79296.0 35 107679.0 36 145497.0 37 177099.0 38 91038.0 39 3272.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.10954453113031 19.758009156732946 15.171683768387584 19.960762543749166 2 15.770765497831752 22.078435000780946 39.378763595701905 22.7720359056854 3 19.448758034302905 27.95783215830151 30.53487740506814 22.05853240232745 4 12.53327444403328 17.85818024104823 37.057186285159496 32.55135902975899 5 12.353590720531619 39.67052316385305 32.86352377067935 15.112362344935985 6 29.481080930524968 37.52598773279537 17.22034215274918 15.77258918393048 7 27.120901639344265 35.7612804392806 20.308073372592713 16.80974454878243 8 27.646147142911143 32.979824004679415 19.357694672192302 20.01633418021714 9 28.155666237929967 16.489551656803734 20.454421110955266 34.90036099431103 10-11 24.951406669969224 27.740275862892204 25.823152110552066 21.48516535658651 12-13 27.297962163850542 25.744616933358007 27.198885486858416 19.758535415933043 14-15 23.707530920628226 26.011227767910388 27.660228464734292 22.62101284672709 16-17 20.516257649334555 29.697249746203497 28.062457068475066 21.72403553598688 18-19 20.396074863879107 26.55380748051881 32.9885318739496 20.06158578165249 20-21 21.82536662762272 25.062781172362854 33.22754164894768 19.884310551066743 22-23 21.39986938955783 25.32599142825453 33.155654720901 20.118484461286638 24-25 21.168224113130897 25.699358784336273 33.472283288801705 19.660133813731125 26-27 20.837737166628784 25.7978502496072 33.508591391747274 19.855821192016748 28-29 20.251034038210175 26.010363261254057 33.755304481727514 19.983298218808255 30-31 20.333022970806027 25.84285310700743 33.610272203852475 20.21385171833407 32-33 20.250745591608787 26.121968640387088 33.26989918845412 20.35738657955 34-35 20.913373780057757 25.703664576687128 33.190884466209674 20.19207717704544 36-37 21.1868739872134 25.491136220979214 32.96454388916024 20.357445902647147 38-39 20.793153788570248 25.78376536654638 33.093702669296256 20.329378175587117 40-41 20.567661611368752 26.163006997041627 33.0406293955328 20.228701996056827 42-43 20.721288449228343 26.518484328347448 32.08872636767915 20.671500854745066 44-45 20.69075287258272 26.49530732320205 31.83618979215339 20.97775001206184 46-47 21.434582535049866 26.914189252368985 30.800859858228836 20.850368354352312 48-49 21.546230641901033 27.23891435483614 30.036985309331676 21.177869693931147 50-51 21.51772311689707 27.67129992071774 29.733981650682555 21.07699531170264 52-53 21.73571151817435 27.830900254805517 29.35023349297432 21.08315473404581 54-55 21.002297368744347 27.824870769899547 29.488585524139783 21.68424633721633 56-57 21.698706373817476 28.12594823306561 28.74863578796986 21.426709605147046 58-59 21.91622544472436 27.16383613153844 28.992969463048023 21.926968960689177 60-61 21.471123223133056 27.718552333733403 29.641167257152606 21.169157185980936 62-63 21.81926157001795 27.691944992521993 29.582659185511716 20.906134251948334 64-65 21.577867242049933 27.779800505947318 29.50107585926985 21.141256392732902 66-67 21.646823827720688 28.010259884240575 29.04022732099541 21.30268896704333 68-69 21.648706156908503 27.90265413658741 29.2540942103593 21.19454549614478 70-71 21.584456461618657 28.160825205823954 29.037044332231094 21.217674000326294 72-73 21.68504927866382 28.11278758321914 28.771378335293868 21.430784802823172 74-75 21.398695105676346 28.853564235728555 28.4563857202759 21.291354938319195 76-77 21.52068598485979 28.28638077332458 28.858565360549903 21.334367881265727 78-79 21.468079835974734 28.233873286305066 28.678705266854422 21.619341610865778 80-81 21.248293339235666 28.770781470327105 28.427584114438908 21.55334107599832 82-83 21.43961704915163 28.522655885894654 28.360655819244336 21.677071245709385 84-85 21.160079720788236 28.480385890793265 28.173002267575953 22.18653212084255 86-87 21.163690369609938 28.51923252917607 28.119143399424633 22.19793370178936 88-89 21.33775607853904 28.315616488503448 27.862797411211847 22.483830021745664 90-91 21.048814554831317 28.234705409513804 28.353239978034043 22.363240057620835 92-93 21.097359534885804 28.440396176704795 28.297646824175075 22.164597464234323 94-95 20.850204744868446 28.0082922542129 28.25946718088723 22.882035820031422 96-97 21.038257481369627 27.953690537118746 28.22704707790512 22.781004903606505 98-99 20.85486970742363 28.253832778261888 27.992610984446063 22.898686529868414 100-101 20.555857025829674 28.580409914464788 28.08038927300789 22.78334378669765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1742.0 1 1232.5 2 1117.0 3 1395.5 4 1124.5 5 1017.0 6 1428.0 7 2604.5 8 3875.0 9 4428.5 10 3929.5 11 3120.5 12 2889.5 13 2980.0 14 3096.0 15 3140.5 16 3138.5 17 3147.0 18 3224.5 19 3347.0 20 3509.0 21 3815.5 22 4272.5 23 4776.5 24 5310.5 25 6035.5 26 7182.0 27 8372.5 28 9334.5 29 10803.0 30 13002.0 31 14874.5 32 17453.5 33 20092.5 34 22130.0 35 23976.5 36 26834.5 37 29344.5 38 29379.0 39 28983.0 40 28730.5 41 29418.5 42 29774.5 43 30233.0 44 31900.5 45 33631.5 46 33806.5 47 34812.5 48 42270.0 49 41445.0 50 32887.0 51 29183.5 52 27110.5 53 26936.0 54 26676.0 55 25293.5 56 23432.0 57 21341.0 58 20205.5 59 18821.0 60 16023.5 61 13446.5 62 11317.0 63 8540.5 64 5907.0 65 4546.5 66 3555.5 67 2840.0 68 2216.0 69 1613.0 70 1243.5 71 1030.5 72 929.5 73 863.0 74 714.5 75 601.5 76 472.0 77 301.0 78 203.0 79 149.5 80 96.5 81 68.0 82 49.0 83 28.5 84 13.5 85 7.0 86 4.0 87 1.0 88 0.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011936949035196095 2 0.009251135502276974 3 0.006067949092891348 4 5.968474517598047E-4 5 0.005172677915251641 6 0.0 7 1.9894915058660156E-4 8 0.002785288108212422 9 2.9842372587990233E-4 10-11 0.0 12-13 0.0 14-15 6.465847394064551E-4 16-17 0.0047250423264317875 18-19 0.0017408050676327635 20-21 4.4763558881985354E-4 22-23 2.4868643823325196E-4 24-25 3.978983011732031E-4 26-27 2.4868643823325196E-4 28-29 0.0 30-31 5.968474517598047E-4 32-33 0.003978983011732031 34-35 8.952711776397071E-4 36-37 0.0010942203282263087 38-39 0.005421364353484893 40-41 0.0 42-43 0.001143957615872959 44-45 0.004526093175845186 46-47 0.0 48-49 0.0017905423552794142 50-51 0.0012931694788129102 52-53 5.471101641131543E-4 54-55 1.4921186293995116E-4 56-57 0.012235372761075995 58-59 0.0024371270946858693 60-61 0.0036308219982054786 62-63 4.973728764665039E-5 64-65 0.0038795084364387305 66-67 7.460593146997559E-4 68-69 0.0020889660811593165 70-71 0.005371627065838242 72-73 0.002934499971152373 74-75 0.006615059257004501 76-77 0.0011936949035196093 78-79 0.0070626948458243554 80-81 0.002934499971152373 82-83 0.0035313474229121777 84-85 0.0023376525193925684 86-87 5.968474517598047E-4 88-89 0.0023873898070392186 90-91 0.00895271177639707 92-93 0.0023376525193925684 94-95 6.465847394064551E-4 96-97 1.4921186293995116E-4 98-99 5.968474517598047E-4 100-101 0.0023376525193925684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1005282.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.51157009094952 #Duplication Level Percentage of deduplicated Percentage of total 1 72.50626696834263 32.27367784194482 2 14.081283870313566 12.535601079280385 3 5.244791548129812 7.0036171982099935 4 2.4980263295234035 4.447642962224734 5 1.4224449571760607 3.165762920592996 6 0.8756734607470864 2.338656037489696 7 0.6026446562290517 1.877726189897293 8 0.4435502017599018 1.5794492715592534 9 0.29901876617547524 1.1978815292216023 >10 1.7683016980130501 14.918836766710713 >50 0.1568205222420545 4.846339765412579 >100 0.09310080681882023 7.648842019179404 >500 0.005159803726921804 1.575542821590458 >1k 0.0022433929247486105 1.868722801574587 >5k 4.4867858494972206E-4 1.5908209336264159 >10k+ 2.2433929247486103E-4 1.130879861485102 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11325 1.1265495651966315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9657 0.9606259736074056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6274 0.6241034853901691 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3167 0.3150359799538836 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2793 0.2778324887941891 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2378 0.23655054004746928 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1934 0.19238382861724374 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1762 0.17527420166679597 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1728 0.17189206610682375 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1644 0.16353620178218647 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1168 0.11618630394257531 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1086 0.10802938876852465 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1054 0.10484620235913904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.973728764665039E-5 0.0 18-19 0.0 0.0 0.0 9.947457529330078E-5 0.0 20-21 0.0 0.0 0.0 2.9842372587990233E-4 0.0 22-23 9.947457529330078E-5 0.0 0.0 6.465847394064551E-4 0.0 24-25 9.947457529330078E-5 0.0 0.0 0.0011936949035196093 0.0 26-27 9.947457529330078E-5 0.0 0.0 0.0020889660811593165 0.0 28-29 9.947457529330078E-5 0.0 0.0 0.003879508436438731 0.0 30-31 9.947457529330078E-5 0.0 0.0 0.009698771091096826 0.0 32-33 9.947457529330078E-5 0.0 0.0 0.01516987273222837 0.0 34-35 9.947457529330078E-5 0.0 0.0 0.01984517777101351 0.0 36-37 9.947457529330078E-5 0.0 0.0 0.023028364180399132 0.0 38-39 9.947457529330078E-5 0.0 0.0 0.026311025165078057 0.0 40-41 9.947457529330078E-5 0.0 0.0 0.0314837030803297 0.0 42-43 9.947457529330078E-5 0.0 0.0 0.03705427929675454 0.0 44-45 9.947457529330078E-5 0.0 0.0 0.04252538093788609 0.0 46-47 9.947457529330078E-5 0.0 0.0 0.05132888085134321 0.0 48-49 9.947457529330078E-5 0.0 0.0 0.06082870279185343 0.0 50-51 9.947457529330078E-5 0.0 0.0 0.07400908401821579 0.0 52-53 9.947457529330078E-5 0.0 0.0 0.1013645922238735 0.0 54-55 9.947457529330078E-5 0.0 0.0 0.13409172749536946 0.0 56-57 9.947457529330078E-5 0.0 0.0 0.17492604065326944 0.0 58-59 9.947457529330078E-5 0.0 0.0 0.23729659936216904 0.0 60-61 9.947457529330078E-5 0.0 0.0 0.3253315984967402 0.0 62-63 9.947457529330078E-5 0.0 0.0 0.3875032080550532 0.0 64-65 9.947457529330078E-5 0.0 0.0 0.4456460973139875 0.0 66-67 9.947457529330078E-5 0.0 0.0 0.5584502656965906 0.0 68-69 9.947457529330078E-5 0.0 0.0 0.7011962812424772 0.0 70-71 9.947457529330078E-5 0.0 0.0 0.8132046530227339 0.0 72-73 9.947457529330078E-5 0.0 0.0 0.9120326435766283 0.0 74-75 9.947457529330078E-5 0.0 0.0 1.1066546501379713 0.0 76-77 9.947457529330078E-5 0.0 0.0 1.2506440978750242 0.0 78-79 9.947457529330078E-5 0.0 0.0 1.366034605215253 0.0 80-81 9.947457529330078E-5 0.0 0.0 1.4853046209919207 0.0 82-83 9.947457529330078E-5 0.0 0.0 1.6896751359320072 0.0 84-85 9.947457529330078E-5 0.0 9.947457529330078E-5 1.8910614136132946 0.0 86-87 9.947457529330078E-5 0.0 9.947457529330078E-5 2.1855558937691115 0.0 88-89 9.947457529330078E-5 0.0 9.947457529330078E-5 2.5748496441794444 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 50 0.0016135095 38.01059 2 TATCACG 55 0.0025759502 34.55508 2 ATCACGC 65 0.005840034 29.234552 3 GGTATCA 10360 0.0 28.20806 1 GTATCAA 14450 0.0 27.359825 1 GTACATG 9060 0.0 25.856907 1 TACATGG 9140 0.0 25.316135 2 AGCCCGA 95 0.0012276573 24.995773 9 CCCTATA 210 1.2605597E-9 24.887886 2 CATGGGG 2410 0.0 24.635214 4 GAGTACT 9520 0.0 24.347649 12-13 ACATGGG 8920 0.0 24.232395 3 AGTACTT 9730 0.0 23.089922 12-13 GTACTTT 10170 0.0 22.605251 14-15 TAAGGTG 465 0.0 22.471552 5 AGGTAAG 360 0.0 22.436808 2 ATCAACG 17650 0.0 22.34 3 TATCAAC 17645 0.0 22.322739 2 TCAACGC 17780 0.0 22.172247 4 GTAAGGT 410 0.0 22.01066 4 >>END_MODULE