FastQCFastQC Report
Thu 26 May 2016
SRR936706_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936706_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002254
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103101.0286813522320688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76530.763578893174784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48560.48450791915023533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25290.25233124537292945No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21720.21671153220640677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.18887427737878823No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18260.1821893452158834No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14800.14766715822536003No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14540.14507300544572532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.13519526986173167No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.11973012829083246No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11310.11284564591410959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG753.3376637E-828.5014618-19
GTATCAA126550.028.4257681
TACCGGC856.3906744E-427.9530727
GTACATG91500.026.4866811
TCGCGTA1109.4480594E-525.920129
TACATGG94150.025.3853242
ATATAGG4550.025.0631082
GGTATCA88800.025.044441
GAGTACT70550.024.71562612-13
ACATGGG91700.024.4572583
GTAGCAC1951.5637852E-824.366913
TATCAAC152200.023.6016432
ATCAACG152950.023.5480443
TCAACGC153100.023.4640734
CAACGCA153350.023.3661795
GTACTTT76350.023.02131814-15
AACGCAG156000.022.938796
AGTACTT72800.022.874912-13
CATGGGA64200.022.6486954
CGACCGT953.2878415E-722.50115418-19
Produced by FastQC (version 0.11.5)