##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936706_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1002254 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.581067274363583 31.0 31.0 34.0 30.0 34.0 2 31.59013483607948 31.0 31.0 34.0 30.0 34.0 3 31.612225044749135 33.0 31.0 34.0 28.0 34.0 4 35.29019889169811 37.0 35.0 37.0 33.0 37.0 5 35.23720533916552 37.0 35.0 37.0 33.0 37.0 6 35.38387574407286 37.0 35.0 37.0 33.0 37.0 7 35.243070119949635 37.0 35.0 37.0 32.0 37.0 8 35.23779101904308 37.0 35.0 37.0 32.0 37.0 9 36.74763183783752 39.0 37.0 39.0 32.0 39.0 10-11 36.62773708062028 39.0 37.0 39.0 32.0 39.0 12-13 36.54664935235978 39.0 37.0 39.0 32.0 39.0 14-15 37.57829901402239 40.0 37.0 41.0 32.0 41.0 16-17 37.548137996954864 40.0 37.0 41.0 32.0 41.0 18-19 37.60528019843273 40.0 37.0 41.0 32.0 41.0 20-21 37.56512820103487 40.0 37.0 41.0 32.0 41.0 22-23 37.45832792884838 40.0 37.0 41.0 32.0 41.0 24-25 37.31030806562009 39.0 36.5 41.0 31.5 41.0 26-27 37.10037824743029 39.0 36.0 41.0 31.0 41.0 28-29 37.16680003272623 39.0 36.0 41.0 31.0 41.0 30-31 37.02379586412227 39.0 36.0 41.0 31.0 41.0 32-33 36.78936676730649 39.0 35.0 41.0 31.0 41.0 34-35 36.589583578613805 39.0 35.0 41.0 30.0 41.0 36-37 36.31186605391448 39.0 35.0 40.0 30.0 41.0 38-39 36.08993678249226 38.0 35.0 40.0 30.0 41.0 40-41 35.89035114851126 38.0 35.0 40.0 29.5 41.0 42-43 35.55728986863609 38.0 35.0 40.0 28.5 41.0 44-45 35.152407473554604 38.0 34.5 40.0 26.5 41.0 46-47 34.61794814488144 38.0 34.0 40.0 24.0 41.0 48-49 34.200213718279 38.0 34.0 40.0 22.5 41.0 50-51 33.419476499969065 37.5 33.0 39.5 21.0 40.5 52-53 33.575067298309605 37.5 33.0 39.5 21.0 40.5 54-55 33.90731990094328 38.0 34.0 40.0 20.5 41.0 56-57 33.83040526652924 38.0 34.0 40.0 20.0 41.0 58-59 33.588529953484844 38.0 33.0 40.0 19.0 41.0 60-61 33.26578442191301 37.0 33.0 40.0 17.5 41.0 62-63 32.95458436683715 36.5 33.0 40.0 14.0 41.0 64-65 32.62886553707942 36.0 32.5 39.0 12.5 41.0 66-67 32.255247172872345 36.0 32.0 39.0 8.0 41.0 68-69 31.875526064251176 35.0 31.5 39.0 7.0 40.5 70-71 31.476396701834066 35.0 31.0 38.0 4.5 40.0 72-73 31.08380859542591 35.0 31.0 37.0 2.0 40.0 74-75 30.65935231987101 35.0 31.0 37.0 2.0 39.0 76-77 30.248926918725193 35.0 30.0 36.0 2.0 39.0 78-79 29.801003039149755 34.5 30.0 36.0 2.0 38.5 80-81 29.398191476412165 34.0 29.0 35.5 2.0 37.0 82-83 29.000855571541745 34.0 29.0 35.0 2.0 37.0 84-85 28.619758065320767 34.0 29.0 35.0 2.0 36.5 86-87 28.280549142233404 34.0 29.0 35.0 2.0 36.0 88-89 27.898230887579395 34.0 27.0 35.0 2.0 36.0 90-91 27.54396440423286 34.0 26.5 35.0 2.0 35.0 92-93 27.198217717265283 34.0 26.0 35.0 2.0 35.0 94-95 26.796092108387697 33.5 25.0 35.0 2.0 35.0 96-97 26.248341737723173 33.0 24.5 35.0 2.0 35.0 98-99 25.630768248368177 33.0 21.5 35.0 2.0 35.0 100-101 23.647629243684733 31.0 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12354494062516963 1101 2 0.20483776073991677 1101 3 0.3593142075061522 1101 4 0.35372946430501884 1101 5 0.38912226163874664 1101 6 0.29716944752428986 1101 7 0.1554158842236788 1101 8 0.14048742545440263 1101 9 0.40772232392508556 1101 10-11 0.3175301951371381 1101 12-13 0.43011463915599535 1101 14-15 0.3058102730296568 1101 16-17 0.35048217631301526 1101 18-19 0.5044063044735978 1101 20-21 0.35699381673655495 1101 22-23 0.39312118886743264 1101 24-25 0.373467332077702 1101 26-27 0.4615794963958706 1101 28-29 0.4592970826963736 1101 30-31 0.23223803368458817 1101 32-33 0.25479701024300283 1101 34-35 0.2794025765970716 1101 36-37 0.47454204160107594 1101 38-39 0.35393227418619233 1101 40-41 0.16525929300424735 1101 42-43 0.160883776755135 1101 44-45 0.26382247789863555 1101 46-47 0.3506741560727704 1101 48-49 0.4640283715455453 1101 50-51 0.42008021385059635 1101 52-53 0.19214992492986482 1101 54-55 0.3767822410675805 1101 56-57 0.509642288742306 1101 58-59 0.5194352821999644 1101 60-61 0.36687951184621426 1101 62-63 0.4357864166040386 1101 64-65 0.44932153708907663 1101 66-67 0.5133231149374922 1101 68-69 0.533927306919491 1101 70-71 0.5744947186087863 1101 72-73 0.5479848693888165 1101 74-75 0.6376764178826697 1101 76-77 0.3191053125530168 1101 78-79 0.43386557392376446 1101 80-81 0.29379971639868074 1101 82-83 0.13703961476172566 1101 84-85 0.2806977005987399 1101 86-87 0.10143962652813343 1101 88-89 0.3226103394884241 1101 90-91 0.27556173841231413 1101 92-93 0.16756769935065563 1101 94-95 0.05023386950658448 1101 96-97 0.042107584210576476 1101 98-99 -0.08807567786103121 1101 100-101 -0.0028463870616626252 1102 1 0.24534156809371765 1102 2 0.1971410756306753 1102 3 0.23806902160572818 1102 4 0.39956005939932027 1102 5 0.35638234600710206 1102 6 0.3078098466769319 1102 7 0.36042952340760337 1102 8 0.3163928800265623 1102 9 0.5246081109041612 1102 10-11 0.596363943948532 1102 12-13 0.6275736757119432 1102 14-15 0.5117630517150786 1102 16-17 0.252261328003506 1102 18-19 0.18472182303793971 1102 20-21 0.1572735760407582 1102 22-23 -0.18836146201718407 1102 24-25 -0.03288158871958302 1102 26-27 -0.021278087027106096 1102 28-29 0.2010495067262994 1102 30-31 0.21652929175703406 1102 32-33 0.14744616931297116 1102 34-35 -0.01942588542431878 1102 36-37 -0.07162999201409548 1102 38-39 -0.20395220210952658 1102 40-41 -0.3040347369615759 1102 42-43 -0.09202230562333824 1102 44-45 0.019999027313595263 1102 46-47 0.09929757141254925 1102 48-49 0.07382398571267146 1102 50-51 -0.0044982197175826855 1102 52-53 0.008635531276944164 1102 54-55 0.04647461597441804 1102 56-57 0.2223680328396469 1102 58-59 -0.04759534079684613 1102 60-61 -0.35555887318628976 1102 62-63 -0.26536970157039974 1102 64-65 -0.17845210018573 1102 66-67 -0.04158093349537495 1102 68-69 0.008474350172040346 1102 70-71 0.15467035752103087 1102 72-73 -0.07022885643972288 1102 74-75 -0.009329019433987895 1102 76-77 -0.01315034780009583 1102 78-79 0.25196952543214834 1102 80-81 0.33488762029658403 1102 82-83 0.19545056722834175 1102 84-85 0.3197740543290486 1102 86-87 0.23477650886401236 1102 88-89 -0.21969315897143105 1102 90-91 -0.506159552298719 1102 92-93 -0.36622370006999816 1102 94-95 -0.22948982228824022 1102 96-97 -0.19936287768598504 1102 98-99 -0.34320588342170666 1102 100-101 -0.22566402584908118 1103 1 0.28997534282596504 1103 2 0.37754808747638435 1103 3 0.3719935668110388 1103 4 0.3291569576718274 1103 5 0.4741218482385676 1103 6 0.16383135382525893 1103 7 0.3136466817853716 1103 8 0.059802233448813524 1103 9 0.22477395384924392 1103 10-11 0.40586141136046194 1103 12-13 0.32556535809648324 1103 14-15 0.4482243797470318 1103 16-17 0.5537142140752422 1103 18-19 0.35529436322151753 1103 20-21 0.2580179217251697 1103 22-23 0.2571369582051659 1103 24-25 0.2710162448352307 1103 26-27 0.38957683560199285 1103 28-29 0.3925288198039212 1103 30-31 0.3569886782130496 1103 32-33 0.4285802983528626 1103 34-35 0.4301470727374195 1103 36-37 0.7828667937488802 1103 38-39 0.5584725356054321 1103 40-41 0.5607077570346704 1103 42-43 0.57964162196307 1103 44-45 0.5666204454730277 1103 46-47 0.8293874292250649 1103 48-49 0.6818316381585987 1103 50-51 0.19079832754315618 1103 52-53 0.6482907966780971 1103 54-55 0.6005084047890819 1103 56-57 0.714205099262216 1103 58-59 0.7729931544668176 1103 60-61 0.9417921340811333 1103 62-63 0.8004940675138954 1103 64-65 0.7344462711975979 1103 66-67 0.6388058536009353 1103 68-69 0.6762989461639428 1103 70-71 0.6774539122529468 1103 72-73 0.5470900459106964 1103 74-75 0.6576053130518993 1103 76-77 0.4004008335499911 1103 78-79 0.4330203584671075 1103 80-81 0.5075189650626442 1103 82-83 0.44144082339352053 1103 84-85 0.42690043204646955 1103 86-87 0.3486969042503709 1103 88-89 0.5690889216009758 1103 90-91 0.6872605876565459 1103 92-93 0.8121713237427706 1103 94-95 0.578349215672862 1103 96-97 0.7608939916126936 1103 98-99 0.9639571235640751 1103 100-101 0.9785235459675086 1104 1 0.23518515845289656 1104 2 0.238406151551235 1104 3 0.1874431421851206 1104 4 0.29903786517352415 1104 5 0.1287838256480569 1104 6 0.1600050281318488 1104 7 0.2611605642453583 1104 8 0.3501735087263427 1104 9 0.42658539417335817 1104 10-11 0.48164787200522596 1104 12-13 0.5597478637313884 1104 14-15 0.5263908611021506 1104 16-17 0.41553462570549726 1104 18-19 0.5483468501343935 1104 20-21 0.4020141455051771 1104 22-23 0.3241398102533708 1104 24-25 0.4064829674930266 1104 26-27 0.45510196718910123 1104 28-29 0.5612613524004715 1104 30-31 0.4023709210713946 1104 32-33 0.30465180475287923 1104 34-35 0.31281543186324967 1104 36-37 0.3343074518991642 1104 38-39 0.18742114306466107 1104 40-41 0.16587285929006867 1104 42-43 0.13486685105745977 1104 44-45 0.11711386731052187 1104 46-47 0.028464927630430736 1104 48-49 -0.1986575207178234 1104 50-51 -0.2413048711092287 1104 52-53 -0.10898248298646962 1104 54-55 0.10618625536593385 1104 56-57 -0.06376581080044019 1104 58-59 -0.06617709515499826 1104 60-61 -0.21033433588487327 1104 62-63 -0.12286568663413533 1104 64-65 0.012789395025478711 1104 66-67 -0.07075924483688567 1104 68-69 -0.03866842150531369 1104 70-71 -0.025509959151964523 1104 72-73 -0.19037159936958759 1104 74-75 -0.20193330094492623 1104 76-77 -0.30259326034013867 1104 78-79 0.04801969834440456 1104 80-81 0.06263881312307973 1104 82-83 0.07662321609176814 1104 84-85 0.11322801833564 1104 86-87 0.1423723720228658 1104 88-89 -0.012771463242607695 1104 90-91 -0.15557811493988538 1104 92-93 -0.12804144255311556 1104 94-95 0.0029604193071968155 1104 96-97 0.19875019939069105 1104 98-99 0.11170212850306527 1104 100-101 0.06262238303809298 1105 1 0.10221813373457067 1105 2 0.07256142737111304 1105 3 -0.0833149715256809 1105 4 0.11686688612367391 1105 5 0.2726725518999231 1105 6 0.24635778032557454 1105 7 0.350495829206082 1105 8 0.0541365976287409 1105 9 0.37911790281188473 1105 10-11 0.40552248636782195 1105 12-13 0.19800934903275902 1105 14-15 0.08045015605814143 1105 16-17 0.1779231880692791 1105 18-19 0.20092789966862057 1105 20-21 -0.1292446900637927 1105 22-23 0.07522509960965351 1105 24-25 0.013426817686813308 1105 26-27 0.0830209889649467 1105 28-29 0.22829332683893 1105 30-31 0.09441146522984667 1105 32-33 -0.009785608768019927 1105 34-35 -0.09361948955804422 1105 36-37 -0.046094286565491416 1105 38-39 -0.04673613444163749 1105 40-41 -0.1083957119812311 1105 42-43 0.05050452579843068 1105 44-45 0.19133639979413886 1105 46-47 -0.05444492391949751 1105 48-49 0.018968329060612632 1105 50-51 -0.2752259496178766 1105 52-53 -0.280725926837917 1105 54-55 -0.1117736258339761 1105 56-57 -0.03292107410977252 1105 58-59 -0.10561782396902686 1105 60-61 -0.005407935392462093 1105 62-63 -0.2066575312632395 1105 64-65 -0.10992524205626353 1105 66-67 -0.42012608640089866 1105 68-69 -0.219667162944976 1105 70-71 -0.03825194427443179 1105 72-73 -0.16859450216519534 1105 74-75 -0.2336387627965344 1105 76-77 -0.05396128790335908 1105 78-79 0.035879470202193176 1105 80-81 0.04668912551126425 1105 82-83 0.020542217850749722 1105 84-85 -0.2587190971091218 1105 86-87 0.02058977018081265 1105 88-89 -0.04965503765405188 1105 90-91 -0.1098270696842576 1105 92-93 0.02022221958223369 1105 94-95 0.10481873493811733 1105 96-97 0.25909604076204573 1105 98-99 -0.1861500619674068 1105 100-101 -0.15757313168306197 1106 1 -0.012942242714185426 1106 2 0.032586551755059645 1106 3 -0.08547122052118183 1106 4 0.11952788156010286 1106 5 0.24850727306878184 1106 6 0.2301468189008844 1106 7 0.16975316562175635 1106 8 0.3040599300325084 1106 9 0.46139678521117844 1106 10-11 0.4283820269877481 1106 12-13 0.2911368048935046 1106 14-15 0.4198239403446351 1106 16-17 0.28533992010973463 1106 18-19 0.16826750367287957 1106 20-21 0.24986984046554994 1106 22-23 0.12995837890645134 1106 24-25 -0.06116749561911661 1106 26-27 0.062273825231628166 1106 28-29 -0.09834885832221829 1106 30-31 0.13603654026852752 1106 32-33 0.12453645421148707 1106 34-35 -0.010642128434987796 1106 36-37 -0.06961321195076664 1106 38-39 -0.2340452799085213 1106 40-41 -0.42430790868812807 1106 42-43 -0.32967944168235164 1106 44-45 -0.34563866607918214 1106 46-47 -0.2087400902556027 1106 48-49 0.01087892030895432 1106 50-51 0.15609639049434776 1106 52-53 0.18293971382953345 1106 54-55 0.4406418161058525 1106 56-57 0.28943163505810077 1106 58-59 0.22443769813948222 1106 60-61 0.21250178883875748 1106 62-63 0.2835542103031088 1106 64-65 0.16400395270376578 1106 66-67 0.21193851766852134 1106 68-69 0.005896723967495632 1106 70-71 0.14773625977517924 1106 72-73 0.2425731648724323 1106 74-75 0.11017652954373958 1106 76-77 0.10385971910488223 1106 78-79 0.3290263861666922 1106 80-81 0.2314936513753949 1106 82-83 0.27031786196715046 1106 84-85 0.21176018613355652 1106 86-87 0.3015228647803738 1106 88-89 0.479871760385695 1106 90-91 0.43352682707120493 1106 92-93 0.45002554222119784 1106 94-95 0.6045563710393864 1106 96-97 0.7488551611653556 1106 98-99 0.48682941022940796 1106 100-101 0.671035479586596 1107 1 0.5097052087185325 1107 2 0.43862168508766075 1107 3 0.47704192631565334 1107 4 0.4031476456298364 1107 5 0.48473860954506165 1107 6 0.32279027269829186 1107 7 0.5782915563791491 1107 8 0.5965382944513635 1107 9 0.6410919879491104 1107 10-11 0.6872254243596814 1107 12-13 0.6932346163626306 1107 14-15 0.7995140588082208 1107 16-17 0.7685952114934835 1107 18-19 0.9884975321946712 1107 20-21 0.9260558969927146 1107 22-23 0.872756404612943 1107 24-25 0.7977897496213515 1107 26-27 0.9819881488287479 1107 28-29 0.5663955871080688 1107 30-31 0.39359740451541825 1107 32-33 0.7148909876395919 1107 34-35 0.6164368163886778 1107 36-37 0.5900908234676905 1107 38-39 0.8244438133261056 1107 40-41 0.8925036930277628 1107 42-43 0.9158356800461505 1107 44-45 0.639585535465443 1107 46-47 0.7683751921758386 1107 48-49 0.9651264327529603 1107 50-51 0.8235978086081204 1107 52-53 0.8750051554186982 1107 54-55 0.737160683249769 1107 56-57 0.946391159249778 1107 58-59 1.0361635301410956 1107 60-61 0.9908473659686976 1107 62-63 1.1836063490380013 1107 64-65 0.9686456957923895 1107 66-67 1.0374252870906986 1107 68-69 0.8813913191844911 1107 70-71 1.05324352580136 1107 72-73 0.9846125370941472 1107 74-75 0.9125607551339918 1107 76-77 1.0909396807834106 1107 78-79 0.9516421356535254 1107 80-81 0.9553223492129845 1107 82-83 1.031402327714595 1107 84-85 1.0465207716425766 1107 86-87 1.0618990213281698 1107 88-89 0.943211650106786 1107 90-91 0.9177516197873317 1107 92-93 0.9648447065459429 1107 94-95 1.1954791468936712 1107 96-97 1.329846654476544 1107 98-99 1.6493841284858384 1107 100-101 1.5314910903824064 1108 1 0.04442695953651565 1108 2 0.073915630725498 1108 3 -0.079051233141751 1108 4 0.08766141579738473 1108 5 0.40066388063005576 1108 6 0.17813432585110434 1108 7 0.24780753532939315 1108 8 0.0922162863075826 1108 9 0.28092359829268787 1108 10-11 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-3.076559527111261 2316 54-55 -2.862597936594863 2316 56-57 -2.441026440671802 2316 58-59 -2.622008474662003 2316 60-61 -2.9145757232071823 2316 62-63 -2.611972840806878 2316 64-65 -2.815198375077806 2316 66-67 -3.347501318638713 2316 68-69 -3.0099318606621033 2316 70-71 -2.6121980651021595 2316 72-73 -2.6255578993505786 2316 74-75 -2.5246626379113195 2316 76-77 -2.9204021233188264 2316 78-79 -3.1700220396105188 2316 80-81 -3.3697719973452074 2316 82-83 -3.0568944105901075 2316 84-85 -3.0707495496258304 2316 86-87 -3.122716975927201 2316 88-89 -3.540005690055441 2316 90-91 -3.4185319835160186 2316 92-93 -3.974247851103481 2316 94-95 -3.8763406389273882 2316 96-97 -4.284611649245857 2316 98-99 -4.773715462270648 2316 100-101 -5.209092020358401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1710.0 3 631.0 4 608.0 5 691.0 6 824.0 7 1012.0 8 1151.0 9 1594.0 10 2162.0 11 3215.0 12 4300.0 13 5849.0 14 6853.0 15 9184.0 16 12106.0 17 15873.0 18 12929.0 19 7139.0 20 6937.0 21 6932.0 22 7525.0 23 8541.0 24 9514.0 25 10943.0 26 12913.0 27 15159.0 28 17956.0 29 21649.0 30 26268.0 31 32266.0 32 40425.0 33 52548.0 34 70497.0 35 98952.0 36 135360.0 37 174854.0 38 142556.0 39 22622.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.19649552085693 18.856109587072606 14.42476666244216 20.522628229628307 2 15.418426595860387 21.176026845717946 40.35399148448718 23.051555073934487 3 19.167175569472217 27.901444485688852 30.050810452664056 22.88056949217487 4 12.035445574992464 17.076598454496274 37.82431722951189 33.063638740999366 5 12.472760196895237 39.34480724251835 33.001115047551735 15.181317513034676 6 29.27168477246202 36.93262767156577 18.109689071527626 15.68599848444459 7 27.429444558662965 35.24827890432724 20.473004397302137 16.849272139707658 8 27.29334999361267 32.73685008942259 19.82017676928973 20.149623147675015 9 28.352659415228022 16.417922363037622 20.562019758507137 34.66739846322722 10-11 24.69287880111304 27.474675883754884 26.286869392695372 21.545575922436704 12-13 27.033615332784304 25.50822290434479 27.23715405155848 20.22100771131242 14-15 23.795065144897563 25.76186173044767 27.44972608255895 22.993347042095813 16-17 20.763023952095807 29.564071856287427 27.742714570858283 21.930189620758483 18-19 20.675210557282508 26.971293745187385 32.011244360228744 20.342251337301363 20-21 22.247588194992307 25.5348186648982 31.80269231939968 20.41490082070981 22-23 21.656886454909824 25.921444633989232 31.805937181452908 20.615731729648036 24-25 21.28580294585431 26.21075969196445 32.14277874562722 20.360658616554016 26-27 20.956637654667745 26.213470174979967 32.252110046705894 20.577782123646394 28-29 20.484774347847996 26.475108993409258 32.48978911727187 20.550327541470875 30-31 20.499320482706736 26.476827562259153 32.27871783767819 20.745134117355928 32-33 20.354526215802817 26.6443932036171 31.96273543536023 21.038345145219854 34-35 21.07419916486652 26.405583520830945 31.86358488777582 20.656632426526713 36-37 21.236201989158662 26.08137739383795 31.80777952119499 20.8746410958084 38-39 21.03931585041986 26.327894681436494 31.80291734456486 20.82987212357878 40-41 20.676572069438265 26.672147864627714 31.942828577529347 20.708451488404673 42-43 20.649252752197693 26.918681020449604 31.132822380167713 21.299243847184993 44-45 20.708433816684444 27.05522335490836 30.80597542103231 21.430367407374888 46-47 21.289013877643058 27.301844349089798 30.090194452634567 21.31894732063257 48-49 21.33219190212427 27.55867919247733 29.447478449748843 21.66165045564956 50-51 21.35462631436897 27.970909148960903 29.117491378058837 21.55697315861129 52-53 21.48104653158313 28.137259988585612 28.808753157913642 21.572940321917617 54-55 20.82505384267313 28.062347246203572 29.104677540134432 22.007921370988864 56-57 21.606621251163034 28.354889483675116 28.21954097248379 21.81894829267806 58-59 21.653609891187163 27.52130134385924 28.643104770333622 22.181983994619973 60-61 21.300509056658747 27.887863857775436 29.31795188007735 21.493675205488472 62-63 21.65261566199364 27.952494483614977 29.16562525099988 21.2292646033915 64-65 21.31921803791074 28.09815185749671 29.072685955463985 21.50994414912856 66-67 21.29713517137885 28.22762176918533 28.743723957917595 21.731519101518224 68-69 21.354761201535847 28.147126675341056 28.93181292058145 21.56629920254165 70-71 21.32071706190222 28.23428745341243 28.757066093230023 21.68792939145533 72-73 21.28138941079156 28.374271234842237 28.537410859411562 21.806928494954644 74-75 20.970196164514775 28.973329209155672 28.38568378200395 21.670790844325598 76-77 21.168826374275483 28.434803171761857 28.76616787348873 21.630202580473927 78-79 21.028216720624073 28.449085984665683 28.668126269812316 21.85457102489793 80-81 20.96717854444674 28.8841683158732 28.334266469859692 21.81438666982037 82-83 21.0392532503168 28.645144231248942 28.409914089861406 21.90568842857285 84-85 20.871037704454277 28.51006760967301 28.29098852017588 22.327906165696835 86-87 21.137615118310116 28.546746552874225 28.124215768595423 22.191422560220243 88-89 21.11916734476395 28.21920760653029 28.00817009523629 22.65345495346947 90-91 20.73889575768099 28.23263151907412 28.48794102884194 22.540531694402947 92-93 21.016562335032777 28.300616809018724 28.46028262845864 22.222538227489864 94-95 20.57891000237982 27.96124235601214 28.55435145406024 22.905496187547804 96-97 20.744119588367443 27.711609756292255 28.576353428866064 22.96791722647424 98-99 20.510617190802044 28.086240941093344 28.354708435817557 23.04843343228706 100-101 20.437566755507643 27.95605865500754 28.773595264476587 22.832779325008236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 800.0 1 639.5 2 752.5 3 1021.5 4 926.5 5 899.5 6 1315.5 7 2422.5 8 3811.0 9 4385.5 10 3639.0 11 2844.5 12 2818.5 13 2888.0 14 2859.5 15 2826.5 16 2807.0 17 2859.5 18 2913.0 19 2988.5 20 3202.0 21 3558.5 22 3964.0 23 4449.0 24 4939.5 25 5679.5 26 6799.5 27 7989.0 28 9084.5 29 10358.0 30 12393.0 31 14795.5 32 17804.5 33 20195.0 34 22256.5 35 24204.5 36 26986.0 37 29741.5 38 29809.5 39 29158.5 40 28486.0 41 29120.0 42 30014.5 43 30656.0 44 32226.5 45 34263.5 46 34484.0 47 35334.5 48 42480.5 49 41497.0 50 33272.5 51 29576.5 52 27344.5 53 27177.0 54 26999.0 55 25564.0 56 23546.0 57 21292.5 58 20053.5 59 18571.0 60 16067.0 61 13746.5 62 11691.0 63 8849.0 64 6015.0 65 4635.0 66 3505.0 67 2686.0 68 2078.5 69 1494.5 70 1181.5 71 992.5 72 824.0 73 782.0 74 653.5 75 492.0 76 398.5 77 258.5 78 169.5 79 133.0 80 81.5 81 51.0 82 37.5 83 20.5 84 11.5 85 8.0 86 10.0 87 13.5 88 7.5 89 3.0 90 3.5 91 2.5 92 1.0 93 1.5 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016462892639989464 2 0.008281333873449245 3 0.00977796047708465 4 0.03791454062543028 5 0.05018687877524061 6 0.06395584352868634 7 0.02753792950689147 8 0.026939278865437306 9 0.015365366463990168 10-11 0.04903946504578679 12-13 0.04779227620942396 14-15 0.021800860859622413 16-17 0.025342877154892873 18-19 0.03287589772652442 20-21 0.025492539815256413 22-23 0.014517278055263436 24-25 0.0031928034210888657 26-27 0.0024943776727256763 28-29 0.0014467390501808923 30-31 0.007183807697449947 32-33 0.0014966266036354058 34-35 0.0018957270312715139 36-37 0.007133920143995435 38-39 0.007533020571631543 40-41 0.003991004276361083 42-43 9.478635156357569E-4 44-45 0.0014966266036354058 46-47 0.0030431407607253253 48-49 3.492128741815947E-4 50-51 0.0014467390501808923 52-53 9.977510690902707E-4 54-55 0.002843590546907271 56-57 0.002644040333089217 58-59 0.002843590546907271 60-61 7.982008552722164E-4 62-63 0.0012471888363628381 64-65 0.003142915867634352 66-67 0.0029932532072708116 68-69 0.006984257483631894 70-71 0.010875486653083948 72-73 0.004489879810906218 74-75 0.0033923536349069194 76-77 0.003492128741815947 78-79 0.03826375349961188 80-81 0.025642202475619956 82-83 0.004390104703997191 84-85 0.010426498671993328 86-87 0.007832345892358624 88-89 0.006235944181814191 90-91 0.013669189646536706 92-93 0.016063792212353354 94-95 0.007732570785449596 96-97 0.004938867791996839 98-99 0.02698916641889182 100-101 0.04629564960578855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1002254.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.49789769878719 #Duplication Level Percentage of deduplicated Percentage of total 1 74.5379309269421 35.40395017847162 2 13.311865648815205 12.64571265534848 3 4.77784929471933 6.808133910624038 4 2.3143040417606637 4.396983064777509 5 1.2766282971499914 3.0318580128703596 6 0.7796108400455034 2.221792555520812 7 0.541386986906977 1.800032060369356 8 0.36948198504214164 1.4039694021661169 9 0.27493238434999095 1.175283923934556 >10 1.5835929446613835 14.31434306510073 >50 0.14452473382848954 4.756093333657787 >100 0.08091972061265443 6.912107986230008 >500 0.004436851469040033 1.330478919803832 >1k 0.0021127864138285875 1.9966215559222387 >5k 2.112786413828587E-4 0.7680008427559757 >10k+ 2.112786413828587E-4 1.0346385324466365 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10310 1.0286813522320688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7653 0.763578893174784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4856 0.48450791915023533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2529 0.25233124537292945 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2172 0.21671153220640677 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.18887427737878823 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1826 0.1821893452158834 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1480 0.14766715822536003 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1454 0.14507300544572532 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1355 0.13519526986173167 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1200 0.11973012829083246 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1131 0.11284564591410959 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.988755345451353E-5 0.0 20-21 0.0 0.0 0.0 1.995502138180541E-4 0.0 22-23 0.0 0.0 0.0 4.489879810906217E-4 0.0 24-25 0.0 0.0 0.0 0.001047638622544784 0.0 26-27 0.0 0.0 0.0 0.002095277245089568 0.0 28-29 0.0 0.0 0.0 0.003342466081452406 0.0 30-31 0.0 0.0 0.0 0.006235944181814191 0.0 32-33 0.0 0.0 0.0 0.012072787935992275 0.0 34-35 0.0 0.0 0.0 0.016462892639989464 0.0 36-37 4.988755345451353E-5 0.0 0.0 0.019057045419624167 0.0 38-39 9.977510690902705E-5 0.0 0.0 0.021401760431986303 0.0 40-41 9.977510690902705E-5 0.0 0.0 0.02574197758252898 0.0 42-43 9.977510690902705E-5 0.0 0.0 0.030431407607253252 0.0 44-45 9.977510690902705E-5 0.0 0.0 0.03686690200288549 0.0 46-47 9.977510690902705E-5 0.0 0.0 0.04469924789524412 0.0 48-49 9.977510690902705E-5 0.0 0.0 0.052431818680693715 0.0 50-51 9.977510690902705E-5 0.0 0.0 0.06265876713886899 0.0 52-53 9.977510690902705E-5 0.0 0.0 0.08575670438830875 0.0 54-55 9.977510690902705E-5 0.0 0.0 0.11194766995192834 0.0 56-57 9.977510690902705E-5 0.0 0.0 0.14836558397372324 0.0 58-59 9.977510690902705E-5 0.0 0.0 0.20593582066023183 0.0 60-61 9.977510690902705E-5 0.0 0.0 0.2865042194892712 0.0 62-63 9.977510690902705E-5 0.0 0.0 0.33898592572341946 0.0 64-65 9.977510690902705E-5 0.0 0.0 0.39476021048556553 0.0 66-67 9.977510690902705E-5 0.0 0.0 0.5068575430978575 0.0 68-69 9.977510690902705E-5 0.0 0.0 0.6394586601799543 0.0 70-71 9.977510690902705E-5 0.0 0.0 0.7509074545973377 0.0 72-73 9.977510690902705E-5 0.0 0.0 0.8448956053056411 0.0 74-75 9.977510690902705E-5 0.0 0.0 1.035565834608792 0.0 76-77 9.977510690902705E-5 0.0 0.0 1.1646748229490727 0.0 78-79 9.977510690902705E-5 0.0 0.0 1.2679919461533704 0.0 80-81 9.977510690902705E-5 0.0 0.0 1.3684654788107604 0.0 82-83 9.977510690902705E-5 0.0 0.0 1.5552444789444593 0.0 84-85 9.977510690902705E-5 0.0 0.0 1.7342410207392538 0.0 86-87 9.977510690902705E-5 0.0 0.0 2.007325488349261 0.0 88-89 9.977510690902705E-5 0.0 0.0 2.3979949194515564 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 75 3.3376637E-8 28.50146 18-19 GTATCAA 12655 0.0 28.425768 1 TACCGGC 85 6.3906744E-4 27.953072 7 GTACATG 9150 0.0 26.486681 1 TCGCGTA 110 9.4480594E-5 25.92012 9 TACATGG 9415 0.0 25.385324 2 ATATAGG 455 0.0 25.063108 2 GGTATCA 8880 0.0 25.04444 1 GAGTACT 7055 0.0 24.715626 12-13 ACATGGG 9170 0.0 24.457258 3 GTAGCAC 195 1.5637852E-8 24.36691 3 TATCAAC 15220 0.0 23.601643 2 ATCAACG 15295 0.0 23.548044 3 TCAACGC 15310 0.0 23.464073 4 CAACGCA 15335 0.0 23.366179 5 GTACTTT 7635 0.0 23.021318 14-15 AACGCAG 15600 0.0 22.93879 6 AGTACTT 7280 0.0 22.8749 12-13 CATGGGA 6420 0.0 22.648695 4 CGACCGT 95 3.2878415E-7 22.501154 18-19 >>END_MODULE