FastQCFastQC Report
Thu 26 May 2016
SRR936706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002254
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117461.1719584057534318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96660.9644261833826554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64240.6409552867835897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30870.3080057550281665No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26640.26580088480564806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.24335148575111695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.20563649533950476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.1839852971402459No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16370.16333185001007727No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16290.1625336491548051No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12800.12771213684355465No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.1125463205933825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT3500.035.2843974
AGGTAAG3450.030.291752
GGTAAGG3800.030.0018753
TAAGGTG4300.029.8185185
CCGTAGA650.00584193529.2325972
GGTATCA95350.028.2865771
GTATCAA135100.027.956591
GTACATG86600.027.1545751
CGTACAC700.00838850627.1445543
TACATGG90300.025.5663812
ACATGGG89100.024.6844843
ATATAGG5250.024.4300982
GAGTACT93650.023.35021812-13
CATGGGG25050.023.132874
ATCAACG165950.022.928523
TATCAAC166150.022.8437392
TCAACGC167850.022.7233124
TGCACCG2303.6343408E-922.7120115
CATGGGA59750.022.5765654
CAACGCA169150.022.5160985
Produced by FastQC (version 0.11.5)