##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936706_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1002254 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.552661301426586 31.0 31.0 34.0 30.0 34.0 2 31.737737140485347 31.0 31.0 34.0 30.0 34.0 3 31.982570286574063 33.0 31.0 34.0 30.0 34.0 4 35.81379869773531 37.0 35.0 37.0 35.0 37.0 5 35.65062948114949 37.0 35.0 37.0 33.0 37.0 6 35.77641496067863 37.0 35.0 37.0 33.0 37.0 7 35.71273050544074 37.0 35.0 37.0 33.0 37.0 8 35.70302438304063 37.0 35.0 37.0 33.0 37.0 9 37.31548190378886 39.0 37.0 39.0 34.0 39.0 10-11 37.21865116028472 39.0 37.0 39.0 33.0 39.0 12-13 37.0838185729366 39.0 37.0 39.0 33.0 39.0 14-15 38.121001762028385 40.0 37.0 41.0 33.0 41.0 16-17 38.066337475330606 40.0 37.0 41.0 33.0 41.0 18-19 38.21934459727774 40.0 37.5 41.0 33.5 41.0 20-21 38.32058989038707 40.0 38.0 41.0 34.0 41.0 22-23 38.22385343435896 40.0 37.5 41.0 33.5 41.0 24-25 38.098250543275455 40.0 37.0 41.0 33.0 41.0 26-27 37.867820432744594 40.0 37.0 41.0 33.0 41.0 28-29 37.74184637826339 40.0 37.0 41.0 33.0 41.0 30-31 37.59978558329526 39.0 36.5 41.0 33.0 41.0 32-33 37.297874091797084 39.0 35.5 41.0 32.5 41.0 34-35 37.073156106136764 39.0 35.0 41.0 32.0 41.0 36-37 36.780011354407165 39.0 35.0 41.0 31.0 41.0 38-39 36.57158863920722 39.0 35.0 41.0 31.0 41.0 40-41 36.26359286168975 39.0 35.0 40.5 30.0 41.0 42-43 35.906150536690305 38.5 35.0 40.0 30.0 41.0 44-45 35.43645473103625 38.0 35.0 40.0 28.0 41.0 46-47 34.78658354069926 38.0 34.5 40.0 24.5 41.0 48-49 34.14506352681057 38.0 34.0 40.0 21.5 41.0 50-51 33.88248986783789 38.0 33.0 40.0 20.0 41.0 52-53 33.58644215937277 38.0 33.0 40.0 18.5 41.0 54-55 33.302340524457875 38.0 33.0 40.0 16.5 41.0 56-57 33.02453020890912 37.0 33.0 40.0 13.5 41.0 58-59 32.659925028984674 37.0 32.0 40.0 10.0 41.0 60-61 32.28880752783226 36.0 31.5 39.0 8.0 41.0 62-63 31.986811726368764 36.0 31.0 39.0 7.0 40.0 64-65 31.54622331265328 35.0 31.0 39.0 3.5 40.0 66-67 31.14321070307527 35.0 30.5 38.0 2.0 40.0 68-69 30.70123142436947 35.0 30.0 38.0 2.0 40.0 70-71 30.231549587230383 34.5 29.0 37.0 2.0 39.0 72-73 29.832764448932107 34.0 29.0 36.5 2.0 39.0 74-75 29.48433830146849 34.0 29.0 36.0 2.0 39.0 76-77 28.072478633160856 32.5 27.0 35.0 2.0 37.0 78-79 28.82929077858507 34.0 29.0 35.0 2.0 37.0 80-81 28.65639548457776 34.0 29.0 35.0 2.0 37.0 82-83 28.32050009279085 34.0 28.5 35.0 2.0 36.5 84-85 27.870878539771354 34.0 27.0 35.0 2.0 36.0 86-87 27.47334807344246 33.0 26.5 35.0 2.0 36.0 88-89 27.050656819528783 33.0 26.0 35.0 2.0 35.0 90-91 26.59167935473443 33.0 25.0 35.0 2.0 35.0 92-93 26.153616747850343 33.0 24.5 35.0 2.0 35.0 94-95 25.643726041502454 32.0 23.0 34.0 2.0 35.0 96-97 24.99302222789832 32.0 19.0 34.0 2.0 35.0 98-99 24.181345247811432 32.0 4.5 34.0 2.0 35.0 100-101 21.537039014062305 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2694791920041517 1101 2 0.33427715635943756 1101 3 0.006865940453945285 1101 4 0.01127378912672583 1101 5 0.006980442189480129 1101 6 0.0597951135885495 1101 7 0.1832218784418842 1101 8 0.08973664738854126 1101 9 0.1939325896870585 1101 10-11 0.11985567351847948 1101 12-13 0.10334654176122626 1101 14-15 0.09731005567323336 1101 16-17 0.08947613310904501 1101 18-19 0.11646985764073037 1101 20-21 -0.015696228430769565 1101 22-23 -0.006869308163274468 1101 24-25 -0.05382950799791075 1101 26-27 -0.006133530411048582 1101 28-29 0.02271092299947952 1101 30-31 -0.03669039416378439 1101 32-33 -0.056485514234559275 1101 34-35 0.020465802478994988 1101 36-37 -0.0273121873232256 1101 38-39 -0.048409355011905575 1101 40-41 0.011671041274375682 1101 42-43 -0.059390583234772976 1101 44-45 -0.2291059866473617 1101 46-47 -0.3067488387222568 1101 48-49 -0.3223945008955269 1101 50-51 -0.4240386265095353 1101 52-53 -0.2888203004233034 1101 54-55 -0.2334368683618635 1101 56-57 -0.3643312693194716 1101 58-59 -0.6191871701587317 1101 60-61 -0.2690327437091611 1101 62-63 -0.5252604353395292 1101 64-65 -0.5841290753403996 1101 66-67 -0.4751971577778207 1101 68-69 -0.6408881103112734 1101 70-71 -0.7618877640591855 1101 72-73 -0.6722160825821284 1101 74-75 -0.41668725880914437 1101 76-77 -0.22455677003155472 1101 78-79 -0.2532567417151981 1101 80-81 -0.555930116620214 1101 82-83 -0.4842629264581326 1101 84-85 -0.5661977124465523 1101 86-87 -0.5659034057372843 1101 88-89 -0.48020269227669843 1101 90-91 -0.6729811172849374 1101 92-93 -0.5492042817873681 1101 94-95 -0.7035770968791262 1101 96-97 -0.7783915649415825 1101 98-99 -1.0163856031363885 1101 100-101 -0.9947674515260445 1102 1 0.40025675398625893 1102 2 0.4966917508369413 1102 3 0.1301832543240593 1102 4 0.13681008624432422 1102 5 0.046696717792777065 1102 6 0.049308912477584954 1102 7 0.12548425742396319 1102 8 -0.06261322062528052 1102 9 -0.04983468875422403 1102 10-11 0.14063239108273962 1102 12-13 0.11218502665532526 1102 14-15 0.2228489227581889 1102 16-17 0.17543713569002506 1102 18-19 0.07866416920155217 1102 20-21 0.15747661655981915 1102 22-23 -0.013097807632760805 1102 24-25 0.007654391156570739 1102 26-27 -0.15874480081934195 1102 28-29 -0.06415580950729804 1102 30-31 -0.09337065718159465 1102 32-33 0.15571815935021505 1102 34-35 0.13692681056704714 1102 36-37 0.39958288825216925 1102 38-39 0.32704598813381125 1102 40-41 0.4234917897222985 1102 42-43 0.2568439005872847 1102 44-45 0.35535892779108025 1102 46-47 0.4222422288054659 1102 48-49 0.25435573866214156 1102 50-51 0.23528466437038986 1102 52-53 -0.07422215153408729 1102 54-55 0.24966926761877772 1102 56-57 0.1817831235991605 1102 58-59 0.23358894529888374 1102 60-61 0.23865586435980646 1102 62-63 0.32565906226856 1102 64-65 0.09871859535132543 1102 66-67 0.24673788079004666 1102 68-69 0.17127848231585574 1102 70-71 -0.14877982910138954 1102 72-73 -0.07517117790956362 1102 74-75 -0.41172795607799983 1102 76-77 -0.12855490496952626 1102 78-79 0.059672764080083596 1102 80-81 -0.038208355972290775 1102 82-83 0.11773283676710378 1102 84-85 0.32765015282841503 1102 86-87 0.2180467809524913 1102 88-89 0.02221234276664319 1102 90-91 0.2747989816220908 1102 92-93 0.1570991138737945 1102 94-95 0.03580691865816377 1102 96-97 -0.18280897169765709 1102 98-99 -0.5489709535881495 1102 100-101 -0.33862908448330487 1103 1 0.37538630474213974 1103 2 0.3831297829538478 1103 3 0.30729662827668136 1103 4 0.049519318767515585 1103 5 0.055830813873818386 1103 6 -0.027126408024763293 1103 7 -0.1205453246428334 1103 8 0.05804463803818294 1103 9 -0.12805278346297655 1103 10-11 0.012122835263177478 1103 12-13 0.15924839304238958 1103 14-15 0.18512452167294668 1103 16-17 -0.005145906381947896 1103 18-19 0.10541065121875448 1103 20-21 0.06013602129300466 1103 22-23 0.1441739635609096 1103 24-25 0.123684152998905 1103 26-27 0.049527421311324815 1103 28-29 0.3804354932511984 1103 30-31 0.369606066379049 1103 32-33 -0.048280648138636195 1103 34-35 0.09800802566999778 1103 36-37 0.14520797533095475 1103 38-39 0.23839716618801532 1103 40-41 0.36431454750920267 1103 42-43 0.4012931806611135 1103 44-45 0.46517856797031243 1103 46-47 0.6647085096538277 1103 48-49 0.5001572304995818 1103 50-51 0.2515906813082367 1103 52-53 0.17529957059583978 1103 54-55 0.2866431057818346 1103 56-57 0.09486801211584606 1103 58-59 0.19964132889796105 1103 60-61 0.10537102048737523 1103 62-63 0.08043734658059876 1103 64-65 -0.043788734247669936 1103 66-67 2.8896956365542792E-5 1103 68-69 0.017556903706751115 1103 70-71 -0.009946662310127863 1103 72-73 0.276285153860222 1103 74-75 0.08477054054896271 1103 76-77 0.18903733112371057 1103 78-79 0.3111619048877827 1103 80-81 0.22877107021411902 1103 82-83 0.2712405573779222 1103 84-85 0.3920142941979172 1103 86-87 0.4489677261524072 1103 88-89 0.5268172185791897 1103 90-91 0.3037125080843026 1103 92-93 0.6039525923059692 1103 94-95 0.5031177311088193 1103 96-97 0.23070887626915493 1103 98-99 0.264617531188609 1103 100-101 0.2717403692537026 1104 1 0.20765946510741884 1104 2 0.3971267702492689 1104 3 0.047259184208158445 1104 4 0.13840285244411632 1104 5 -0.10732765852271342 1104 6 0.08625642655803034 1104 7 -0.04936262335710495 1104 8 0.044824686744100006 1104 9 -0.056364253214248095 1104 10-11 0.05935819366208506 1104 12-13 0.10442281654848529 1104 14-15 0.2843409639774279 1104 16-17 -0.044324586486482076 1104 18-19 0.11067294262395677 1104 20-21 -0.043875059224461666 1104 22-23 0.1098408226970875 1104 24-25 0.0987637256211471 1104 26-27 0.0701073430828103 1104 28-29 0.043378829272747055 1104 30-31 0.05881154905598862 1104 32-33 0.014761070025549827 1104 34-35 0.07606371775695209 1104 36-37 -0.08693570554505925 1104 38-39 -0.039796038206652895 1104 40-41 0.17134835374489654 1104 42-43 0.19733077425140522 1104 44-45 0.0589668928652074 1104 46-47 0.11237111855419357 1104 48-49 0.180751293212829 1104 50-51 0.21312501325611066 1104 52-53 -0.02301083341515664 1104 54-55 -0.051345285602536705 1104 56-57 -0.06190039297997885 1104 58-59 -0.061807028032767164 1104 60-61 -0.48139216725310874 1104 62-63 -0.42316986609503005 1104 64-65 -0.3684004946119934 1104 66-67 -0.2604587030760861 1104 68-69 -0.09480631966445685 1104 70-71 -0.3482372785466019 1104 72-73 -0.17732770564817102 1104 74-75 -0.14217208922082492 1104 76-77 -0.09205802717975686 1104 78-79 0.07550486010601176 1104 80-81 -0.15853484973447252 1104 82-83 0.02915328120325711 1104 84-85 0.1449462729276476 1104 86-87 -0.022764706232734966 1104 88-89 0.13726250713234123 1104 90-91 -0.061517874739145384 1104 92-93 0.03690042988715092 1104 94-95 0.08439871909776642 1104 96-97 -0.16647224072208644 1104 98-99 -0.0799856078141481 1104 100-101 -0.054633043866989084 1105 1 0.315667962266275 1105 2 0.36422154759918257 1105 3 0.16297167197910767 1105 4 0.04406837564741295 1105 5 -0.009675386483522175 1105 6 0.04501918418132789 1105 7 0.03942968416198056 1105 8 0.07949139344449918 1105 9 0.01332195741820641 1105 10-11 -0.0013304365088302461 1105 12-13 0.01628355922419189 1105 14-15 0.07082357557769825 1105 16-17 0.08692349001796629 1105 18-19 0.04548927538430547 1105 20-21 -0.048609047609680545 1105 22-23 0.02466531232959568 1105 24-25 -0.09893408478411203 1105 26-27 -0.013802100623514946 1105 28-29 0.21729034972872796 1105 30-31 -0.06660286258581749 1105 32-33 -0.10033840127902494 1105 34-35 -0.18037483172098234 1105 36-37 -0.04270844407727026 1105 38-39 -0.21896824078732635 1105 40-41 -0.19902743785953447 1105 42-43 -0.06800777347505971 1105 44-45 -0.20527698259986238 1105 46-47 0.011781371692457299 1105 48-49 -0.02413882671320522 1105 50-51 -0.22268840955261027 1105 52-53 -0.19969218745593764 1105 54-55 -0.19511766383119067 1105 56-57 -0.43342840533038185 1105 58-59 -0.2899955268430219 1105 60-61 -0.29361646622499293 1105 62-63 -0.5172466257650505 1105 64-65 -0.4208872146736482 1105 66-67 -0.5655544962818624 1105 68-69 -0.49537129565673155 1105 70-71 -0.5614656734693249 1105 72-73 -0.8732852999213456 1105 74-75 -0.7657699704357483 1105 76-77 -0.654122816764982 1105 78-79 -0.6063120706581557 1105 80-81 -0.7452028675394722 1105 82-83 -0.3905328043237972 1105 84-85 -0.3455870013559341 1105 86-87 -0.5147717221436388 1105 88-89 -0.33019382482733306 1105 90-91 -0.7952435386109578 1105 92-93 -0.7945713350748278 1105 94-95 -0.630536113498696 1105 96-97 -0.41841450207727604 1105 98-99 -0.4909005536560187 1105 100-101 -0.2537107668486023 1106 1 0.3085215866671618 1106 2 0.34062356554244033 1106 3 0.018914778975798185 1106 4 0.014772231962346893 1106 5 -0.1203277951491728 1106 6 -0.0925455054604214 1106 7 0.030310366794623178 1106 8 -0.010907738495873787 1106 9 0.08894357307595158 1106 10-11 -0.028465553047922754 1106 12-13 -0.040900476888204196 1106 14-15 0.04822466134723413 1106 16-17 0.05476740608584407 1106 18-19 -0.01809878549455135 1106 20-21 -0.037318278040764596 1106 22-23 -0.07963172182896727 1106 24-25 -0.015150519362983061 1106 26-27 -0.1623591426182429 1106 28-29 -0.1974644051825578 1106 30-31 0.04041380794680549 1106 32-33 0.14901633121839808 1106 34-35 -0.0924564286309959 1106 36-37 0.15330345091114594 1106 38-39 -0.10942827242737252 1106 40-41 0.15140776477314688 1106 42-43 -0.05400648945570907 1106 44-45 0.10252495524421334 1106 46-47 0.2654885648939356 1106 48-49 0.045185620997749254 1106 50-51 -0.15840100119535805 1106 52-53 -0.07393039823840297 1106 54-55 0.0605281553305872 1106 56-57 -0.054027185772632436 1106 58-59 -0.19456326494476883 1106 60-61 0.05388538639425633 1106 62-63 -0.42835608624236343 1106 64-65 -0.22672229203190497 1106 66-67 -0.2069243312097946 1106 68-69 0.010564904740640202 1106 70-71 -0.1360843119049413 1106 72-73 -0.10696378946979124 1106 74-75 -0.06477663639003595 1106 76-77 -0.1792463319547224 1106 78-79 -0.2868366768299637 1106 80-81 -0.3618449050123367 1106 82-83 -0.0569140850940002 1106 84-85 0.12962047896894546 1106 86-87 -0.1537177316495537 1106 88-89 -0.001702929810249998 1106 90-91 0.03062979870127336 1106 92-93 0.25490728183304867 1106 94-95 0.11697381853862865 1106 96-97 0.27234316489953514 1106 98-99 0.3710234946015625 1106 100-101 0.21271099788743086 1107 1 0.6241005010848646 1107 2 0.4896157327692059 1107 3 0.3856554500800726 1107 4 0.26985725398922256 1107 5 0.040563567865021355 1107 6 0.1735484658861992 1107 7 0.2156192065532494 1107 8 0.22908097640892322 1107 9 0.24351448524461006 1107 10-11 0.3484636050660299 1107 12-13 0.42322199231455215 1107 14-15 0.5585676704201745 1107 16-17 0.4810733418266011 1107 18-19 0.3851968211939649 1107 20-21 0.34604348402915264 1107 22-23 0.37793602114202685 1107 24-25 0.4253774276967661 1107 26-27 0.4435388149988526 1107 28-29 0.4837745228131496 1107 30-31 0.4970275017917487 1107 32-33 0.4360654881759274 1107 34-35 0.3010584299115351 1107 36-37 0.36133002471836306 1107 38-39 0.3161912862775509 1107 40-41 0.494182562324319 1107 42-43 0.3261541736134106 1107 44-45 0.40900551088561343 1107 46-47 0.44531635753131127 1107 48-49 0.6112876673683303 1107 50-51 0.5528401299858956 1107 52-53 0.4106288599759438 1107 54-55 0.5662913298318486 1107 56-57 0.41647255851827225 1107 58-59 0.10278852596594135 1107 60-61 0.14524431323354037 1107 62-63 0.2448721189190728 1107 64-65 0.5471227053910717 1107 66-67 0.5091324053041681 1107 68-69 0.4404308294355559 1107 70-71 0.18273657509157815 1107 72-73 0.4894734302097383 1107 74-75 0.7802980476123444 1107 76-77 0.4311716452418075 1107 78-79 0.3595061846993275 1107 80-81 0.47441354759375187 1107 82-83 0.5377765368124443 1107 84-85 0.6987755822345463 1107 86-87 1.035108728110412 1107 88-89 0.9956123739552041 1107 90-91 0.9939666976727537 1107 92-93 1.193042130745365 1107 94-95 1.1643308432611619 1107 96-97 1.0416855887824674 1107 98-99 0.9177375081576322 1107 100-101 1.0511855959556051 1108 1 0.30587182993658146 1108 2 0.3385945232335352 1108 3 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-1.277515649789855 2316 80-81 -1.0970944044732924 2316 82-83 -1.091698798301632 2316 84-85 -1.2048567602557156 2316 86-87 -1.7407636712328411 2316 88-89 -1.6383621892324847 2316 90-91 -1.666223955891585 2316 92-93 -1.7320368998131208 2316 94-95 -1.7261566956414747 2316 96-97 -1.477518224288822 2316 98-99 -1.7865472959200979 2316 100-101 -2.4987947995647737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 15.0 5 60.0 6 106.0 7 270.0 8 572.0 9 1106.0 10 1869.0 11 2972.0 12 4418.0 13 6082.0 14 7817.0 15 11020.0 16 14815.0 17 19479.0 18 15629.0 19 8735.0 20 7035.0 21 7024.0 22 7393.0 23 8175.0 24 9480.0 25 11061.0 26 13366.0 27 16155.0 28 19983.0 29 24078.0 30 28946.0 31 35979.0 32 44440.0 33 56390.0 34 75098.0 35 101927.0 36 139102.0 37 180293.0 38 114476.0 39 6882.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.09236682233335 19.760159745664904 15.233696577987393 19.913776854014355 2 15.768970172800381 22.129165714536605 39.47546198681021 22.626402125852803 3 19.490983399637003 27.975258604260393 30.622116987807907 21.911641008294694 4 12.536523155281287 17.813420563135164 37.177977535265796 32.47207874631775 5 12.279122857080141 39.75120079341165 32.910320138408025 15.05935621110018 6 29.345754668976127 37.64225435867555 17.212103917769348 15.79988705457898 7 27.069984255488126 35.76678167410656 20.249956597828493 16.913277472576812 8 27.67073017418738 32.9354634653491 19.31207059288364 20.081735767579875 9 28.162819355128388 16.433599576937084 20.50298588626193 34.90059518167259 10-11 24.91704909805393 27.660527643066924 25.9193943224716 21.50302893640755 12-13 27.32539953754169 25.720314990483935 27.183618898431284 19.770666573543096 14-15 23.731808503632813 25.912493240236508 27.76072732061932 22.59497093551136 16-17 20.4705094716509 29.567405068974534 28.152544165451072 21.809541293923495 18-19 20.313413565822636 26.43601322618817 33.19906929780275 20.051503910186437 20-21 21.839024838015114 24.940085048301132 33.39043795285427 19.83045216082949 22-23 21.29013558082442 25.219869223397307 33.43354184886208 20.056453346916197 24-25 21.058534064219252 25.57874550762581 33.726081412496235 19.636639015658705 26-27 20.762700872233985 25.63087800098578 33.764694378870026 19.84172674791021 28-29 20.2107948683667 25.84689110744382 33.996022964238605 19.946291059950873 30-31 20.30075225479382 25.706071368171823 33.77862985761586 20.21454651941849 32-33 20.23042054018565 25.978910628600165 33.48710999979046 20.30355883142372 34-35 20.93009913713971 25.482914408409897 33.43748253925176 20.149503915198633 36-37 21.145498402308856 25.35806491041206 33.21401577985038 20.2824209074287 38-39 20.76929639058382 25.65932670726518 33.30379988695475 20.267577015196256 40-41 20.647530899305234 25.998072254534243 33.205116308307566 20.14928053785296 42-43 20.690597976741913 26.31303223282835 32.393683155947336 20.6026866344824 44-45 20.655755043233416 26.364102594803207 32.00619815332547 20.973944208637903 46-47 21.504020769611074 26.78298021369272 30.9807045962842 20.73229442041201 48-49 21.554311236441087 27.15284918642784 30.158074428643488 21.134765148487585 50-51 21.521138199619955 27.50218383745764 29.969583422092004 21.0070945408304 52-53 21.669772233488736 27.744260937457128 29.5165542925419 21.069412536512235 54-55 20.99761012627913 27.655878141386726 29.660378488142936 21.68613324419121 56-57 21.703633045538044 27.98450834499914 28.841209039559295 21.470649569903525 58-59 21.961457508621898 27.08815064587533 29.155310904674458 21.79508094082831 60-61 21.485297199133896 27.519831568864188 29.78232670451711 21.21254452748481 62-63 21.90360541760542 27.511240114386748 29.772134494229775 20.813019973778054 64-65 21.5982507998539 27.646403701182965 29.61081095106271 21.14453454790042 66-67 21.675693258193775 27.86921885726145 29.18449368604224 21.27059419850253 68-69 21.68449483314093 27.891909527833793 29.25244424312025 21.171151395905024 70-71 21.630205080210168 28.082788388433134 29.067530596402865 21.21947593495383 72-73 21.701135640266838 27.977139527505003 28.90479858399168 21.416926248236475 74-75 21.450949559692454 28.790955187008482 28.58102836207189 21.177066891227174 76-77 21.511303923559268 28.20824941718081 29.041831775906086 21.238614883353847 78-79 21.459188067130373 28.155012351074877 28.836953338332638 21.548846243462112 80-81 21.331633531338646 28.69952673321175 28.536365700257715 21.43247403519189 82-83 21.413742263334758 28.445881387348415 28.50375825431395 21.636618095002884 84-85 21.145522806930014 28.370199443702994 28.418644680877602 22.06563306848939 86-87 21.268816635624717 28.37901148394159 28.224990857052195 22.1271810233815 88-89 21.353655937964255 28.244148717710715 27.99323511373203 22.408960230592996 90-91 21.059394453523623 28.155191556780895 28.507940468319216 22.277473521376265 92-93 21.15601728353549 28.31897611864795 28.44744022178641 22.077566376030145 94-95 20.816276434762695 27.91029979728796 28.463504598315343 22.809919169634004 96-97 21.094938474073192 27.83452586643463 28.42885489380959 22.641680765682587 98-99 20.834867539392924 28.134445345842472 28.135393310562222 22.895293804202378 100-101 20.487643270407112 28.42017870008362 28.33815308653802 22.754024942971242 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1820.0 1 1314.0 2 1212.0 3 1454.5 4 1069.5 5 932.0 6 1488.0 7 2773.0 8 4126.0 9 4700.0 10 4085.0 11 3216.0 12 2960.0 13 2955.5 14 3058.5 15 3176.0 16 3171.0 17 3227.0 18 3331.5 19 3352.5 20 3523.0 21 3910.5 22 4333.0 23 4742.5 24 5245.5 25 5999.5 26 7060.0 27 8295.5 28 9379.0 29 10676.5 30 12667.0 31 14847.5 32 17545.5 33 19967.0 34 21956.5 35 23791.0 36 26631.0 37 29184.0 38 29194.0 39 28666.5 40 28445.0 41 29020.5 42 29560.0 43 30376.0 44 31944.5 45 33579.0 46 33898.0 47 34965.5 48 42315.5 49 41378.5 50 32709.5 51 29029.5 52 26876.0 53 26671.0 54 26460.5 55 25083.0 56 23234.0 57 21119.5 58 20101.5 59 18696.0 60 16004.5 61 13489.0 62 11337.0 63 8635.0 64 5946.0 65 4612.5 66 3559.0 67 2750.5 68 2135.5 69 1521.0 70 1213.5 71 1024.5 72 891.5 73 821.0 74 689.5 75 582.0 76 442.0 77 291.5 78 214.0 79 159.5 80 108.5 81 82.5 82 60.5 83 38.0 84 24.0 85 13.0 86 6.0 87 4.0 88 2.5 89 2.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0154112630131682 2 0.011474137294538111 3 0.004689430024724271 4 0.014966266036354058 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003891229169452055 10-11 4.489879810906217E-4 12-13 1.4966266036354057E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010476386225447842 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.988755345451353E-5 32-33 0.0042903295970881635 34-35 5.986506414541623E-4 36-37 0.002644040333089217 38-39 4.988755345451353E-5 40-41 0.004689430024724271 42-43 0.010775711546174922 44-45 0.0019955021381805414 46-47 0.0020453896916350547 48-49 0.009927623137448192 50-51 4.489879810906217E-4 52-53 0.0021451647985440817 54-55 0.010875486653083948 56-57 0.003691678955634001 58-59 9.478635156357569E-4 60-61 0.0063856068421777315 62-63 0.003991004276361083 64-65 0.0195559209541693 66-67 0.0029932532072708116 68-69 9.977510690902705E-5 70-71 0.0011973012829083246 72-73 0.0 74-75 0.0 76-77 0.0013469639432718652 78-79 0.011424249741083596 80-81 0.017610306369443274 82-83 0.012621551023991921 84-85 0.009129422282175975 86-87 0.011524024847992625 88-89 0.03147904622979803 90-91 0.026590065991255712 92-93 0.002843590546907271 94-95 0.00932897249599403 96-97 0.011524024847992625 98-99 0.010675936439265895 100-101 0.012771213684355463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1002254.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.15794335291771 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97127389751832 32.95232653054374 2 13.916761306556408 12.569046374751029 3 5.18702511048307 7.027061583280686 4 2.4286665478217984 4.386943455586519 5 1.4000894638267485 3.1612580348252615 6 0.852165544184918 2.3089226022966494 7 0.5509982526571559 1.741736351673394 8 0.4183310968199473 1.5112777578367291 9 0.29703525132644376 1.20721509478973 >10 1.7237463861307751 14.629758495322594 >50 0.15710963576766826 4.881858577897804 >100 0.088786678793863 7.3698032829805316 >500 0.005340552075241292 1.6700908105028445 >1k 0.002002707028215484 1.7855501571495211 >5k 4.450460062701076E-4 1.6168328003002868 >10k+ 2.225230031350538E-4 1.1803180902627204 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11746 1.1719584057534318 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9666 0.9644261833826554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6424 0.6409552867835897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3087 0.3080057550281665 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2664 0.26580088480564806 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2439 0.24335148575111695 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.20563649533950476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1844 0.1839852971402459 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1637 0.16333185001007727 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1629 0.1625336491548051 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1280 0.12771213684355465 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1128 0.1125463205933825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.988755345451353E-5 0.0 20-21 0.0 0.0 0.0 9.977510690902705E-5 0.0 22-23 0.0 0.0 0.0 3.492128741815947E-4 0.0 24-25 0.0 0.0 0.0 9.478635156357569E-4 0.0 26-27 0.0 0.0 0.0 0.001995502138180541 0.0 28-29 0.0 0.0 0.0 0.0032426909745433795 0.0 30-31 0.0 0.0 0.0 0.0060363939679961365 0.0 32-33 0.0 0.0 0.0 0.012022900382537761 0.0 34-35 0.0 0.0 0.0 0.01656266774689849 0.0 36-37 0.0 0.0 0.0 0.019156820526533194 0.0 38-39 0.0 0.0 0.0 0.021401760431986303 0.0 40-41 0.0 0.0 0.0 0.025991415349801546 0.0 42-43 0.0 0.0 0.0 0.03068084537452582 0.0 44-45 0.0 0.0 0.0 0.03741566509088515 0.0 46-47 0.0 0.0 0.0 0.045397673643607306 0.0 48-49 0.0 0.0 0.0 0.05323001953596593 0.0 50-51 0.0 0.0 0.0 0.06360663065450475 0.0 52-53 0.0 0.0 0.0 0.08690411811776257 0.0 54-55 0.0 0.0 0.0 0.11284564591410959 0.0 56-57 0.0 0.0 0.0 0.14926355993590448 0.0 58-59 0.0 0.0 0.0 0.20678390906895858 0.0 60-61 0.0 0.0 0.0 0.28825028386017915 0.0 62-63 0.0 0.0 0.0 0.3405823274339639 0.0 64-65 0.0 0.0 0.0 0.3958577366615649 0.0 66-67 0.0 0.0 0.0 0.5081047319342202 0.0 68-69 0.0 0.0 0.0 0.6409552867835898 0.0 70-71 0.0 0.0 0.0 0.7528530691820636 0.0 72-73 0.0 0.0 0.0 0.846442119462731 0.0 74-75 0.0 0.0 0.0 1.038209874941881 0.0 76-77 0.0 0.0 0.0 1.1674685259425255 0.0 78-79 0.0 0.0 0.0 1.2711348620210048 0.0 80-81 0.0 0.0 0.0 1.371708169785304 0.0 82-83 0.0 0.0 0.0 1.558137957044821 0.0 84-85 0.0 0.0 0.0 1.7369349486257974 0.0 86-87 0.0 0.0 0.0 2.007574926116533 0.0 88-89 0.0 0.0 0.0 2.3991922207344647 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 350 0.0 35.284397 4 AGGTAAG 345 0.0 30.29175 2 GGTAAGG 380 0.0 30.001875 3 TAAGGTG 430 0.0 29.818518 5 CCGTAGA 65 0.005841935 29.232597 2 GGTATCA 9535 0.0 28.286577 1 GTATCAA 13510 0.0 27.95659 1 GTACATG 8660 0.0 27.154575 1 CGTACAC 70 0.008388506 27.144554 3 TACATGG 9030 0.0 25.566381 2 ACATGGG 8910 0.0 24.684484 3 ATATAGG 525 0.0 24.430098 2 GAGTACT 9365 0.0 23.350218 12-13 CATGGGG 2505 0.0 23.13287 4 ATCAACG 16595 0.0 22.92852 3 TATCAAC 16615 0.0 22.843739 2 TCAACGC 16785 0.0 22.723312 4 TGCACCG 230 3.6343408E-9 22.712011 5 CATGGGA 5975 0.0 22.576565 4 CAACGCA 16915 0.0 22.516098 5 >>END_MODULE