FastQCFastQC Report
Thu 26 May 2016
SRR936705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030491
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102910.9986501580314626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77570.7527479618938934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.4875345830288668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25490.24735781292607117No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22070.21416975014823034No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17680.17156869880474454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.17137461656627762No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15490.15031669369261838No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15430.14973444697721766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13790.1338197034229314No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.125377126049621No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11490.11150024599923727No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10780.1046103265336621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG550.002573509534.561842
CCATGCG753.070553E-431.680159
GTATCAA131950.028.630981
GTACATG93900.026.113351
TACATGG96600.025.1879252
ACATGGG95500.024.5325723
GAGTACT72350.024.43334212-13
GGTATCA92800.024.1697671
AGTACTT74500.023.88768212-13
ATCAACG157600.023.8818823
TAACCCG500.001656067323.74512584-85
TCAACGC158750.023.6789464
TATCAAC159700.023.567842
CAACGCA160550.023.4430665
AACGCAG162750.023.1542476
CATGGGG27150.023.1048764
GTACTTT77650.022.67274714-15
CATGGGA63400.022.4870034
ACGCAGA176450.021.3316367
CGCAGAG177800.021.1953728
Produced by FastQC (version 0.11.5)