##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936705_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1030491 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.570682325221668 31.0 31.0 34.0 30.0 34.0 2 31.569059797708082 33.0 31.0 34.0 30.0 34.0 3 31.596592304056998 33.0 31.0 34.0 28.0 34.0 4 35.26311049781124 37.0 35.0 37.0 33.0 37.0 5 35.2566970502411 37.0 35.0 37.0 33.0 37.0 6 35.411033187092364 37.0 35.0 37.0 33.0 37.0 7 35.291574598904795 37.0 35.0 37.0 33.0 37.0 8 35.2423660177527 37.0 35.0 37.0 32.0 37.0 9 36.73340572600828 39.0 37.0 39.0 32.0 39.0 10-11 36.64779750623732 39.0 37.0 39.0 32.0 39.0 12-13 36.54354040937767 39.0 37.0 39.0 32.0 39.0 14-15 37.557388662297875 40.0 37.0 41.0 32.0 41.0 16-17 37.467605248371896 40.0 37.0 41.0 32.0 41.0 18-19 37.51515588200188 40.0 37.0 41.0 32.0 41.0 20-21 37.48452242668786 40.0 37.0 41.0 32.0 41.0 22-23 37.35491236701728 39.5 37.0 41.0 32.0 41.0 24-25 37.450805004604604 40.0 37.0 41.0 32.0 41.0 26-27 37.403795375214344 40.0 37.0 41.0 32.0 41.0 28-29 37.29949655067341 39.5 36.5 41.0 31.5 41.0 30-31 37.047523947322205 39.0 36.0 41.0 31.0 41.0 32-33 36.78781134430092 39.0 35.0 41.0 31.0 41.0 34-35 36.52780519189396 39.0 35.0 41.0 30.0 41.0 36-37 36.304145305490295 39.0 35.0 40.0 30.0 41.0 38-39 36.06801369444275 38.0 35.0 40.0 30.0 41.0 40-41 35.838807422869294 38.0 35.0 40.0 29.5 41.0 42-43 35.535777605044586 38.0 35.0 40.0 28.5 41.0 44-45 35.13765573886623 38.0 34.5 40.0 26.5 41.0 46-47 34.63274497302742 38.0 34.0 40.0 24.0 41.0 48-49 34.34744990494822 38.0 34.0 40.0 23.0 41.0 50-51 33.38661618587644 37.5 33.0 39.5 21.0 40.5 52-53 33.5055949057294 37.5 33.0 39.5 21.0 40.5 54-55 33.90313112875319 38.0 34.0 40.0 20.5 41.0 56-57 33.806562114564805 38.0 34.0 40.0 20.0 41.0 58-59 33.5458029230726 37.5 33.0 40.0 18.5 41.0 60-61 33.22676714304152 37.0 33.0 40.0 17.5 41.0 62-63 32.90099088686849 36.5 33.0 40.0 13.5 41.0 64-65 32.546054259571406 36.0 32.0 39.0 11.5 41.0 66-67 32.1590280749662 35.5 32.0 39.0 8.0 41.0 68-69 31.75588433086752 35.0 31.0 39.0 7.0 40.0 70-71 31.31918910499946 35.0 31.0 38.0 3.5 40.0 72-73 30.93650599568555 35.0 31.0 37.0 2.0 39.5 74-75 30.52284396467315 35.0 30.5 37.0 2.0 39.0 76-77 30.089350610534204 35.0 30.0 36.0 2.0 39.0 78-79 29.64658934430286 34.0 29.5 36.0 2.0 38.5 80-81 29.179089870750932 34.0 29.0 35.0 2.0 37.0 82-83 28.723781187802707 34.0 29.0 35.0 2.0 37.0 84-85 28.395363472364146 34.0 28.5 35.0 2.0 36.0 86-87 28.06013541117778 34.0 27.5 35.0 2.0 36.0 88-89 27.651921268599146 34.0 26.5 35.0 2.0 36.0 90-91 27.23922382631192 33.5 26.0 35.0 2.0 35.0 92-93 26.886994646241455 33.0 25.5 35.0 2.0 35.0 94-95 26.457234949165013 33.0 25.0 35.0 2.0 35.0 96-97 25.895958819630643 33.0 23.5 35.0 2.0 35.0 98-99 25.225328508448882 33.0 19.0 35.0 2.0 35.0 100-101 22.830594347742966 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2818781078045589 1101 2 0.4539876130124476 1101 3 0.34440631441822234 1101 4 0.4147035338956897 1101 5 0.3779593776182111 1101 6 0.2741840761229497 1101 7 0.28496474769941216 1101 8 0.4084166041430919 1101 9 0.5782551260951294 1101 10-11 0.5526430550233314 1101 12-13 0.5722895086580664 1101 14-15 0.7811158938540004 1101 16-17 0.7418367162336565 1101 18-19 0.6657147653098221 1101 20-21 0.6955968280128673 1101 22-23 0.7011339429719428 1101 24-25 0.5354771412448045 1101 26-27 0.5842509108949869 1101 28-29 0.5818583788299705 1101 30-31 0.6243344554624812 1101 32-33 0.5920751364716779 1101 34-35 0.4681969998363513 1101 36-37 0.5079269854591928 1101 38-39 0.5454451047895716 1101 40-41 0.4530400704262334 1101 42-43 0.5874933260448714 1101 44-45 0.47452536576373205 1101 46-47 0.37480775236717534 1101 48-49 0.519557214245971 1101 50-51 0.5788634756565543 1101 52-53 0.5110577908688683 1101 54-55 0.5773536000107171 1101 56-57 0.225060608619863 1101 58-59 0.2523370316609217 1101 60-61 0.2856054420032308 1101 62-63 0.34310847602204575 1101 64-65 0.5111234735973369 1101 66-67 0.4386469351439075 1101 68-69 0.3327315936311308 1101 70-71 0.276849323978805 1101 72-73 0.11650812835604896 1101 74-75 0.2499697458089294 1101 76-77 0.14987815350772848 1101 78-79 2.50899390724868E-4 1101 80-81 0.2656536840973587 1101 82-83 0.2516742311138245 1101 84-85 0.4489865366179373 1101 86-87 0.3491888096003386 1101 88-89 0.2421876053850056 1101 90-91 0.220597690621247 1101 92-93 0.05652436701788943 1101 94-95 0.3094144988895593 1101 96-97 0.38893839279949916 1101 98-99 0.1080380520651616 1101 100-101 0.8475598737941858 1102 1 0.2691240407355693 1102 2 0.3539959594399953 1102 3 0.2878131809878788 1102 4 0.3565594164941146 1102 5 0.32973807390622767 1102 6 0.34128862782906566 1102 7 0.23630580087341002 1102 8 0.20579364856423155 1102 9 0.36144324368540737 1102 10-11 0.31875837362098736 1102 12-13 0.4668625163113731 1102 14-15 0.35862771493562917 1102 16-17 0.3423614525046972 1102 18-19 0.27588652027322524 1102 20-21 0.4428394223728631 1102 22-23 0.29857775881346527 1102 24-25 0.2333520682135557 1102 26-27 0.2769441579093339 1102 28-29 0.4801796582284439 1102 30-31 0.25569960440991935 1102 32-33 0.24906526954276131 1102 34-35 0.12438043254055486 1102 36-37 0.2822813167107583 1102 38-39 0.20213948401494974 1102 40-41 0.14922938191853774 1102 42-43 0.2884782044954832 1102 44-45 0.09639482037912472 1102 46-47 0.25548069216040403 1102 48-49 0.3606796998846775 1102 50-51 0.29432686568511457 1102 52-53 0.13198954249370587 1102 54-55 0.2012987748683699 1102 56-57 0.2624224843161542 1102 58-59 0.49096030658136414 1102 60-61 0.26556204057997945 1102 62-63 0.41267631251916015 1102 64-65 0.45482246513115854 1102 66-67 0.5089078880156208 1102 68-69 0.5149740246914547 1102 70-71 0.42357117383745546 1102 72-73 0.10871075058585333 1102 74-75 0.23130967402965652 1102 76-77 0.32736239508963294 1102 78-79 0.2417645784596978 1102 80-81 0.35185740224938655 1102 82-83 0.5224936688549136 1102 84-85 0.34917959311599134 1102 86-87 0.21691752893175575 1102 88-89 0.4184757676105306 1102 90-91 0.5777496718091264 1102 92-93 0.4231168835383734 1102 94-95 0.37121108556357996 1102 96-97 0.2126208366334872 1102 98-99 0.5296103012822293 1102 100-101 0.5566342549791621 1103 1 0.4735640583298739 1103 2 0.41487007654391306 1103 3 0.6048683885752411 1103 4 0.565234427127038 1103 5 0.6804551145730642 1103 6 0.49423331118608615 1103 7 0.4622494045798504 1103 8 0.4685355248297256 1103 9 0.6785856957645535 1103 10-11 0.565488704532072 1103 12-13 0.693136195011725 1103 14-15 0.8028515843015427 1103 16-17 0.9136489267403007 1103 18-19 0.6133522131944247 1103 20-21 0.814398081570431 1103 22-23 0.7845419376792151 1103 24-25 0.7929360573952451 1103 26-27 0.8847445425121307 1103 28-29 0.4781806688104737 1103 30-31 0.6393325377514927 1103 32-33 0.8463425143617087 1103 34-35 0.6349406512334141 1103 36-37 0.6513073410156167 1103 38-39 0.7101983304246602 1103 40-41 0.39133611828385995 1103 42-43 0.49725390981266315 1103 44-45 0.5844863107695133 1103 46-47 0.7126752728233896 1103 48-49 0.7494549689793999 1103 50-51 0.7768081717351549 1103 52-53 0.9414490265152367 1103 54-55 1.0694054603410805 1103 56-57 1.0164988014353398 1103 58-59 0.8105408786871138 1103 60-61 0.7607803153928643 1103 62-63 0.9654603206038175 1103 64-65 0.7712815020246779 1103 66-67 0.8956838041736646 1103 68-69 0.870575302823255 1103 70-71 0.7947495924583414 1103 72-73 0.91363352103469 1103 74-75 0.8433377812967571 1103 76-77 0.9126271639462544 1103 78-79 1.0604218130274212 1103 80-81 0.6973481343397268 1103 82-83 0.6738690419159994 1103 84-85 0.8353077189225502 1103 86-87 0.80684733346774 1103 88-89 1.1125006104795645 1103 90-91 1.3321033537084404 1103 92-93 1.1828088377786337 1103 94-95 1.2468251595541844 1103 96-97 1.3081266547505308 1103 98-99 1.3292213362563636 1103 100-101 1.6206208193274279 1104 1 0.28689827799341927 1104 2 0.3575889253864801 1104 3 0.3755289004000204 1104 4 0.4781246571915645 1104 5 0.4073211230880176 1104 6 0.4543546353634227 1104 7 0.41867674147705003 1104 8 0.5786610230039884 1104 9 0.6049161959330576 1104 10-11 0.5999183819594052 1104 12-13 0.3533133270738915 1104 14-15 0.6543434790178821 1104 16-17 0.5743188223285429 1104 18-19 0.6019649312084638 1104 20-21 0.7400661529674224 1104 22-23 0.5597084337788445 1104 24-25 0.7633335301174 1104 26-27 0.3961623565126402 1104 28-29 0.5290744452264633 1104 30-31 0.5703402333650871 1104 32-33 0.5525692675177112 1104 34-35 0.3937914571240029 1104 36-37 0.4827458858675371 1104 38-39 0.6187537873845699 1104 40-41 0.7367962009599225 1104 42-43 0.6483912466789548 1104 44-45 0.8352399491034959 1104 46-47 0.9640781995681991 1104 48-49 0.681815735263406 1104 50-51 0.8102673948770374 1104 52-53 0.9347806799436356 1104 54-55 0.9193015741953445 1104 56-57 0.828448258970333 1104 58-59 0.9748842780520519 1104 60-61 1.0551170518219593 1104 62-63 1.059070317790237 1104 64-65 0.6451603200724207 1104 66-67 0.7813665309376461 1104 68-69 1.0123068468001861 1104 70-71 0.9979316467450339 1104 72-73 0.9282289834362523 1104 74-75 0.7488717282591715 1104 76-77 0.8768795856374396 1104 78-79 0.7295413470238508 1104 80-81 1.0287893845600244 1104 82-83 0.914236771045239 1104 84-85 1.0673204975368442 1104 86-87 1.19402149117062 1104 88-89 1.1221599350341052 1104 90-91 1.1340529967544057 1104 92-93 1.143156639937363 1104 94-95 1.1801130942295828 1104 96-97 1.1305242449629738 1104 98-99 1.24188006408394 1104 100-101 1.4736725521545218 1105 1 0.1718374610815374 1105 2 0.24504776110114435 1105 3 0.18355726392049476 1105 4 0.228204330001887 1105 5 0.30752096905391824 1105 6 0.21140899877767083 1105 7 0.27257008614162714 1105 8 0.382738943012491 1105 9 0.3240197440288739 1105 10-11 0.2569866881154539 1105 12-13 0.23770807929145832 1105 14-15 0.15748723532531272 1105 16-17 0.3447864353311516 1105 18-19 0.3059605226462949 1105 20-21 0.23638958474094096 1105 22-23 0.0883591083419688 1105 24-25 0.18596791159081505 1105 26-27 0.2900912883658364 1105 28-29 0.32720512191419715 1105 30-31 0.15805003843227894 1105 32-33 0.17071408612927996 1105 34-35 0.27774800888477813 1105 36-37 0.06762564631795698 1105 38-39 -0.07740565118769638 1105 40-41 -0.11609293432452006 1105 42-43 0.23343035275002677 1105 44-45 -0.09018132088110065 1105 46-47 0.1601280880262621 1105 48-49 0.32300757411186254 1105 50-51 0.39769834510808977 1105 52-53 0.10495018384759902 1105 54-55 0.22939977093407293 1105 56-57 0.426820941819436 1105 58-59 0.44585515763987615 1105 60-61 0.3210665525390155 1105 62-63 0.38531221772118585 1105 64-65 0.30603632262194935 1105 66-67 0.22409671896547678 1105 68-69 0.2780239684871191 1105 70-71 0.3717000378854145 1105 72-73 0.5289603134410328 1105 74-75 0.4196503971920862 1105 76-77 0.12858757150879185 1105 78-79 0.3051923402782215 1105 80-81 0.13893040543430146 1105 82-83 0.24031434580489375 1105 84-85 0.27413119493951044 1105 86-87 0.31045832391083295 1105 88-89 0.20028078540856598 1105 90-91 0.17222291230122622 1105 92-93 0.20303905614812479 1105 94-95 0.1966501819227915 1105 96-97 0.47911602804046893 1105 98-99 0.47004307465386574 1105 100-101 0.9500528835934112 1106 1 0.11035761104803754 1106 2 0.26078123556265 1106 3 0.1552639169020047 1106 4 0.1872308307360413 1106 5 0.27972388712830565 1106 6 0.11817976528895713 1106 7 0.32784528607320595 1106 8 0.29039799731119587 1106 9 0.4211068372162359 1106 10-11 0.3970777799342997 1106 12-13 0.32062800687416626 1106 14-15 0.38755555418944 1106 16-17 0.3928016422478535 1106 18-19 0.41712568537135297 1106 20-21 0.23211341900799454 1106 22-23 0.38464674252065123 1106 24-25 0.3805261020140591 1106 26-27 0.1401372619363741 1106 28-29 0.35439768702560315 1106 30-31 0.2560708584011948 1106 32-33 0.008095053783847561 1106 34-35 0.24552552531408622 1106 36-37 0.1546622576737846 1106 38-39 0.4770984222844845 1106 40-41 0.01882212148526463 1106 42-43 -0.06186207836089608 1106 44-45 0.2834532818929958 1106 46-47 -0.0073007954931085806 1106 48-49 -0.018214770468951258 1106 50-51 -0.06849058844763789 1106 52-53 0.025533959097423065 1106 54-55 0.15723863891508216 1106 56-57 0.04798713805887189 1106 58-59 -0.03982220411867132 1106 60-61 0.1443378215521136 1106 62-63 0.33960281701697426 1106 64-65 0.23024497135536137 1106 66-67 0.22529461558553265 1106 68-69 0.2091210796362475 1106 70-71 0.3866583765645153 1106 72-73 0.5229458834420306 1106 74-75 0.3328066788211892 1106 76-77 0.4621779469952578 1106 78-79 0.6784803593519051 1106 80-81 0.5857650003679424 1106 82-83 0.9628968131244591 1106 84-85 0.805004764056676 1106 86-87 0.9170426123540025 1106 88-89 0.8589060865532758 1106 90-91 0.8124601216698579 1106 92-93 0.837070278973826 1106 94-95 0.7935238900298174 1106 96-97 1.0510602111364484 1106 98-99 1.413524093627764 1106 100-101 1.9426285083350372 1107 1 0.18198359945985487 1107 2 0.19762890188678028 1107 3 0.22362495204533417 1107 4 0.3467930750810453 1107 5 0.28881646028845154 1107 6 0.2961908532623667 1107 7 0.25954224621413147 1107 8 0.26159065220844724 1107 9 0.34275611435938913 1107 10-11 0.126290548321073 1107 12-13 0.11942378916712215 1107 14-15 0.3168180716559945 1107 16-17 0.3885775013483084 1107 18-19 0.6082382705292773 1107 20-21 0.5095361266990821 1107 22-23 0.6106489282179197 1107 24-25 0.48551795548897303 1107 26-27 0.4153461808861536 1107 28-29 0.5591015576671268 1107 30-31 0.5617236768813996 1107 32-33 0.3328310393140228 1107 34-35 0.45788975744746807 1107 36-37 0.38010352023112404 1107 38-39 0.4496032204880791 1107 40-41 0.31470817162581 1107 42-43 0.6379244264989268 1107 44-45 0.530236809057449 1107 46-47 0.5525430884510811 1107 48-49 0.868522704815085 1107 50-51 0.5867383207516852 1107 52-53 0.6871255853033205 1107 54-55 0.6823428945003016 1107 56-57 0.664145349194321 1107 58-59 0.7337270529073692 1107 60-61 0.7489363945056482 1107 62-63 0.6283885226292796 1107 64-65 0.7702540149950039 1107 66-67 0.5799629641154453 1107 68-69 0.5645877695010313 1107 70-71 0.8419431062249352 1107 72-73 0.8167649454045538 1107 74-75 0.8321302182008949 1107 76-77 0.7637239744089044 1107 78-79 0.6313777675064038 1107 80-81 0.7310952925735563 1107 82-83 1.0854866106665426 1107 84-85 0.959855797731997 1107 86-87 0.7907121189772468 1107 88-89 0.9890076326713064 1107 90-91 0.8460525308120879 1107 92-93 0.8527715960104842 1107 94-95 1.0881181858311422 1107 96-97 0.9862264673052437 1107 98-99 1.095046614827762 1107 100-101 1.7953879964541564 1108 1 0.12706500344821237 1108 2 0.11186095958370856 1108 3 0.17566615030310473 1108 4 0.24246692282774518 1108 5 0.2538806207463864 1108 6 0.2963007202488228 1108 7 -0.05589797590339174 1108 8 0.08966371561659514 1108 9 0.027904332372727936 1108 10-11 0.062081674420440436 1108 12-13 0.15701366129312788 1108 14-15 0.1826774642591502 1108 16-17 0.338639697235557 1108 18-19 0.12690914069442982 1108 20-21 0.25849797678464626 1108 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70-71 -3.3524538811315985 2316 72-73 -2.8655136025661214 2316 74-75 -2.537308580163131 2316 76-77 -2.4886304973993347 2316 78-79 -3.41695924609596 2316 80-81 -2.802616309390153 2316 82-83 -2.654416846985601 2316 84-85 -2.358094686330297 2316 86-87 -3.1708954720559497 2316 88-89 -3.3115589261758593 2316 90-91 -3.167696706685689 2316 92-93 -3.1661553410828027 2316 94-95 -3.6618204763516715 2316 96-97 -5.098864841632519 2316 98-99 -6.17107960376897 2316 100-101 -6.389168491736346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1928.0 3 625.0 4 652.0 5 758.0 6 836.0 7 1043.0 8 1374.0 9 1819.0 10 2261.0 11 3213.0 12 4562.0 13 6036.0 14 7120.0 15 9536.0 16 12711.0 17 16776.0 18 12795.0 19 7436.0 20 7073.0 21 7467.0 22 7945.0 23 8909.0 24 10412.0 25 11763.0 26 13597.0 27 16068.0 28 18863.0 29 22364.0 30 27358.0 31 33722.0 32 42532.0 33 54831.0 34 73598.0 35 102840.0 36 139893.0 37 178165.0 38 140418.0 39 21190.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.15395069578259 18.89519334844462 14.407723259572775 20.54313269620001 2 15.501338020855071 21.068805429345996 40.32824946881979 23.10160708097914 3 19.13506252238489 27.93138892582631 30.04986066772723 22.883687884061573 4 12.057498495057963 17.04497349360157 37.75948113482339 33.13804687651708 5 12.416326990335003 39.373163660231754 33.06848612780634 15.142023221626912 6 29.34071098044775 36.92024334972883 18.077144893286913 15.66190077653652 7 27.324451279780803 35.30451229292523 20.56669210499232 16.804344322301645 8 27.320899282635985 32.71108064787029 19.778625872676383 20.189394196817346 9 28.324349828549682 16.384184630440103 20.60219254472659 34.68927299628363 10-11 24.72517709838281 27.388255192555654 26.281244659862363 21.605323049199175 12-13 27.0189202781343 25.490385492900042 27.24047238559775 20.250221843367903 14-15 23.77809954803845 25.734722694818924 27.446409961308287 23.040767795834345 16-17 20.776487127805414 29.588328614758456 27.68968476812962 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20.693866500011644 26.850511222041938 31.080174910138265 21.37544736780815 44-45 20.717714121572183 26.97454355602223 30.778793283815283 21.52894903859031 46-47 21.392894815752165 27.207281605687406 30.055595073009165 21.344228505551257 48-49 21.373999132443974 27.542138088911877 29.329070599069755 21.75479217957439 50-51 21.42939281591753 27.899298049998716 29.139683052616412 21.531626081467344 52-53 21.59985210366524 28.045715882795065 28.8097984233125 21.544633590227196 54-55 20.919083323466786 27.869903281969066 29.09222276784761 22.118790626716535 56-57 21.571811325570977 28.380848760257184 28.1741642964357 21.873175617736138 58-59 21.75516084623272 27.466314563046488 28.611233638900657 22.16729095182013 60-61 21.374271020663272 27.78962886471871 29.25651629157815 21.579583823039872 62-63 21.747872998065876 27.814401794959632 29.17362566124601 21.26409954572848 64-65 21.455531570190672 27.9918912322736 29.015239844753392 21.537337352782345 66-67 21.35226740617274 28.19988014566911 28.662750778203982 21.78510166995417 68-69 21.42930630682221 28.084620259983282 28.902685342187738 21.583388091006768 70-71 21.338617332091754 28.135643554267176 28.72827374439117 21.797465369249903 72-73 21.32615139615442 28.23746391765583 28.51476846889555 21.921616217294197 74-75 21.06014501649547 28.939005827153498 28.30917443078624 21.6916747255648 76-77 21.224730239958696 28.41509637215992 28.66965610174237 21.690517286139013 78-79 21.101748127214517 28.36036284339253 28.639036428940905 21.898852600452052 80-81 21.061711224912177 28.759054520502875 28.253889070325727 21.92534518425922 82-83 21.136394294736984 28.574208305957683 28.24356546912561 22.045831930179723 84-85 20.94492682727977 28.520812344341756 28.11971635501047 22.414544473368004 86-87 21.154268861641746 28.50158353991894 28.04711947125731 22.29702812718201 88-89 21.215194394127828 28.170516959354902 27.880877642552377 22.73341100396489 90-91 20.761619079828755 28.081385841809812 28.49266266808805 22.66433241027338 92-93 20.996224364014015 28.25558823243941 28.424958021527917 22.323229382018656 94-95 20.65967394811995 27.899927937633294 28.500205025925467 22.940193088321287 96-97 20.728504040478054 27.7221001266031 28.520248004266364 23.02914782865248 98-99 20.569116817744597 28.035414258301035 28.31927381649152 23.07619510746285 100-101 20.536635256073275 27.895868858952312 28.615385511517943 22.95211037345647 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 797.0 1 631.5 2 789.5 3 1063.5 4 896.5 5 894.5 6 1355.0 7 2451.0 8 3881.5 9 4367.5 10 3588.0 11 2805.5 12 2722.5 13 2818.5 14 2842.0 15 2870.5 16 2868.5 17 2885.5 18 2928.5 19 3014.5 20 3208.5 21 3567.0 22 4020.5 23 4547.0 24 4979.0 25 5704.0 26 6917.5 27 8229.0 28 9301.5 29 10622.5 30 12698.0 31 15085.5 32 18173.5 33 20798.0 34 23050.0 35 24769.0 36 27480.5 37 30418.5 38 30486.5 39 29691.5 40 29340.0 41 29847.0 42 30428.5 43 31683.0 44 33404.5 45 35157.5 46 35358.5 47 36204.5 48 43730.5 49 42997.0 50 34345.5 51 30605.5 52 28484.0 53 28195.5 54 28012.5 55 26394.0 56 24325.5 57 21958.0 58 20585.5 59 19272.0 60 16732.0 61 14355.5 62 12172.0 63 9083.5 64 6175.5 65 4866.0 66 3726.0 67 2879.5 68 2264.5 69 1591.0 70 1245.0 71 1052.0 72 884.5 73 823.0 74 672.0 75 541.5 76 427.0 77 251.5 78 177.0 79 135.5 80 82.0 81 57.5 82 42.0 83 24.0 84 18.5 85 14.5 86 5.5 87 7.5 88 8.5 89 3.5 90 4.0 91 6.0 92 5.0 93 2.5 94 1.0 95 1.5 96 1.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034546638447109194 2 0.023095786377561763 3 0.021349046231359615 4 0.05346965669763249 5 0.0571572192285037 6 0.05172291655143033 7 0.05327557445916558 8 0.045609326039722814 9 0.04415370925122102 10-11 0.05385782117456629 12-13 0.047064942828224605 14-15 0.041290996233834164 16-17 0.050364340882162004 18-19 0.05385782117456629 20-21 0.012081619344564873 22-23 0.020572717277491992 24-25 0.006210631630940978 26-27 0.010723043675296534 28-29 0.0061621110713242526 30-31 0.010334879198362722 32-33 0.0037360830904879325 34-35 0.00834553625407694 36-37 0.009461509125261646 38-39 0.011547893188780882 40-41 0.0015526579077352444 42-43 0.001843781265435603 44-45 0.0024260279808363196 46-47 3.881644769338111E-4 48-49 9.704111923345279E-4 50-51 4.366850365505375E-4 52-53 0.0038331242097213855 54-55 0.007084001704042054 56-57 0.017127757544704417 58-59 0.01091712591376344 60-61 0.010868605354146712 62-63 0.004852055961672639 64-65 1.455616788501792E-4 66-67 0.007132522263658781 68-69 0.018825977131289844 70-71 0.00819997457522676 72-73 0.003444959732787574 74-75 0.005482823236690082 76-77 0.00819997457522676 78-79 0.007181042823275506 80-81 0.013149071656132853 82-83 0.007181042823275506 84-85 0.018340771535122577 86-87 0.004512412044355555 88-89 0.006938440025191875 90-91 0.004027206448188291 92-93 0.019505264965924012 94-95 0.012954989417665947 96-97 0.01023783807912927 98-99 0.022125375185227236 100-101 0.04119395511460071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1030491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.893288178863024 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74539013756393 34.58163831583468 2 13.613947698012389 12.768055453097277 3 5.107415851147159 7.185105700714099 4 2.3313757499538514 4.373034995831953 5 1.3331878487936524 3.12587809950196 6 0.8098539261747459 2.278602812573761 7 0.5344257046613822 1.7542685005214695 8 0.3848429948478248 1.4437242768812597 9 0.28514988010112485 1.203445395157526 >10 1.6165951900001203 14.405464155672604 >50 0.1475210015757972 4.811311038013906 >100 0.08342834317902846 7.030207770991432 >500 0.00416101453879165 1.2000104713865734 >1k 0.002080507269395825 1.5883065426157246 >5k 4.1610145387916504E-4 1.2469377101456742 >10k+ 2.0805072693958252E-4 1.0040087610600996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10291 0.9986501580314626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7757 0.7527479618938934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5024 0.4875345830288668 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2549 0.24735781292607117 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2207 0.21416975014823034 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1768 0.17156869880474454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1766 0.17137461656627762 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1549 0.15031669369261838 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1543 0.14973444697721766 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1379 0.1338197034229314 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1292 0.125377126049621 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1149 0.11150024599923727 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1078 0.1046103265336621 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.704111923345279E-5 0.0 14-15 0.0 0.0 0.0 9.704111923345279E-5 0.0 16-17 0.0 0.0 0.0 9.704111923345279E-5 0.0 18-19 0.0 0.0 0.0 9.704111923345279E-5 0.0 20-21 0.0 0.0 0.0 1.9408223846690558E-4 0.0 22-23 0.0 0.0 0.0 4.366850365505375E-4 0.0 24-25 0.0 0.0 0.0 0.0014070962288850655 0.0 26-27 0.0 0.0 0.0 0.0019408223846690558 0.0 28-29 0.0 0.0 0.0 0.0037360830904879325 0.0 30-31 0.0 0.0 0.0 0.00834553625407694 0.0 32-33 0.0 0.0 0.0 0.01494433236195173 0.0 34-35 0.0 0.0 0.0 0.01887449769090657 0.0 36-37 0.0 0.0 0.0 0.02178573126791015 0.0 38-39 4.8520559616726395E-5 0.0 0.0 0.024891047083380642 0.0 40-41 9.704111923345279E-5 0.0 0.0 0.029112335770035837 0.0 42-43 9.704111923345279E-5 0.0 0.0 0.03444959732787574 0.0 44-45 9.704111923345279E-5 0.0 0.0 0.04090283175690035 0.0 46-47 9.704111923345279E-5 0.0 0.0 0.04866612129557657 0.0 48-49 9.704111923345279E-5 0.0 0.0 0.05904952105355603 0.0 50-51 9.704111923345279E-5 0.0 0.0 0.07292640110393978 0.0 52-53 9.704111923345279E-5 0.0 0.0 0.09941862665467238 0.0 54-55 9.704111923345279E-5 0.0 0.0 0.13056882592861072 0.0 56-57 9.704111923345279E-5 0.0 0.0 0.172199466079762 0.0 58-59 9.704111923345279E-5 0.0 0.0 0.2332383300776038 0.0 60-61 9.704111923345279E-5 0.0 0.0 0.31387950016060306 0.0 62-63 9.704111923345279E-5 0.0 0.0 0.37486984359882813 0.0 64-65 9.704111923345279E-5 0.0 0.0 0.4344045702485514 0.0 66-67 9.704111923345279E-5 0.0 0.0 0.5523095301171965 0.0 68-69 9.704111923345279E-5 0.0 0.0 0.6939895641980376 0.0 70-71 9.704111923345279E-5 0.0 0.0 0.8067513447473098 0.0 72-73 9.704111923345279E-5 0.0 0.0 0.9064125742000657 0.0 74-75 9.704111923345279E-5 0.0 0.0 1.1039397723997588 0.0 76-77 9.704111923345279E-5 0.0 0.0 1.2408647916381608 0.0 78-79 9.704111923345279E-5 0.0 0.0 1.3500360507757954 0.0 80-81 9.704111923345279E-5 0.0 0.0 1.459158789353813 0.0 82-83 9.704111923345279E-5 0.0 0.0 1.6564433847554225 0.0 84-85 9.704111923345279E-5 0.0 0.0 1.8517386372127462 0.0 86-87 9.704111923345279E-5 0.0 0.0 2.1526146273960665 0.0 88-89 9.704111923345279E-5 0.0 0.0 2.5483968321897037 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 55 0.0025735095 34.56184 2 CCATGCG 75 3.070553E-4 31.68015 9 GTATCAA 13195 0.0 28.63098 1 GTACATG 9390 0.0 26.11335 1 TACATGG 9660 0.0 25.187925 2 ACATGGG 9550 0.0 24.532572 3 GAGTACT 7235 0.0 24.433342 12-13 GGTATCA 9280 0.0 24.169767 1 AGTACTT 7450 0.0 23.887682 12-13 ATCAACG 15760 0.0 23.881882 3 TAACCCG 50 0.0016560673 23.745125 84-85 TCAACGC 15875 0.0 23.678946 4 TATCAAC 15970 0.0 23.56784 2 CAACGCA 16055 0.0 23.443066 5 AACGCAG 16275 0.0 23.154247 6 CATGGGG 2715 0.0 23.104876 4 GTACTTT 7765 0.0 22.672747 14-15 CATGGGA 6340 0.0 22.487003 4 ACGCAGA 17645 0.0 21.331636 7 CGCAGAG 17780 0.0 21.195372 8 >>END_MODULE