FastQCFastQC Report
Thu 26 May 2016
SRR936705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030491
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119491.1595443337205276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99920.9696348633806603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66610.646390895214029No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30990.3007304285044702No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27360.2655045022227268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.25453885574934665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.19612010197080806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.18146689296655671No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17620.17098645208934382No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17580.17059828761241No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12560.1218836457572167No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.10761860122989915No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.10606594332216389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG2700.029.9080812
GTCGCAC350.00839216927.14346756-57
CGTAAGA700.00839025527.1434672
GGTATCA105100.026.4951821
GTATCAA143000.025.7535861
GTATAGG4450.025.6285041
GTACATG87600.025.1701531
TACATGG90950.023.8680462
GTGTACG1601.8526134E-623.7597581
TATAACG1000.001653313423.7505342
TAAGGTG4000.023.7470765
ACATGGG87900.023.181993
GAGTACT94750.022.8350512-13
AGTACTT97000.022.23191812-13
ATGGGAG19600.022.0508565
TACACCG1100.00287922221.5882515
CATGGGA59350.021.2863434
GTACTTT102650.020.98459614-15
CATATAG2509.489668E-920.8994543
TATCAAC177550.020.8410762
Produced by FastQC (version 0.11.5)